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No products found
because this supplier's products are not listed.
Arun Upadhyay, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 10 nM recombinant MT3 protein (Boster Bio Cat no. PROTP25713) was added at a in additional wells ...
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No products found
Lukas Kunz, et al.,
bioRxiv - Plant Biology 2022
Quote:
... for FLAG tagged effectors or WesternBright ECL HRP substrate (Advansta) for Pm8-HA.
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No products found
because this supplier's products are not listed.
Kathleen M. Mulvaney, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... the N-terminal tag was removed by incubation with HRV 3C protease (AG Scientific) and further purified by Superose 6 column ...
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No products found
because this supplier's products are not listed.
Katherine P. Mueller, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Recombinant sNLS-SpCas9-sNLS Cas9 (Aldevron, 10 mg/ml, total 0.8 µl) was added to the complexed gRNA at a 1:1 molar ratio and incubated for 15 minutes at 37°C to form an RNP ...
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No products found
because this supplier's products are not listed.
Li Jiang, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Myc (db2603) and Flag (db7002) were obtained from Diagnostic Biosystems (Hangzhou, China). MGO (W296902) ...
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No products found
because this supplier's products are not listed.
Jay Lubow, et al.,
bioRxiv - Immunology 2019
Quote:
... CAp24 concentrations were measured by comparison to recombinant CAp24 standards (ViroGen, Watertown, Massachusetts).
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No products found
because this supplier's products are not listed.
Maria Carmen Rodenas, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Thrombin generation assay-calibrated automated thrombogram (TAG-CAT) was conducted as previously described [24] (Diagnostica Stago). Samples were processed in duplicate ...
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No products found
because this supplier's products are not listed.
Mitali Adlakha, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Polyclonal anti-chicken-FLAG (ET-DY100) was purchased from Aves Labs (Tigard, Oregon). Monoclonal mouse-anti-FLAG (clone M2 ...
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No products found
because this supplier's products are not listed.
Mohd Farid Abdul Halim, et al.,
bioRxiv - Microbiology 2023
Quote:
... maripaludis MM1265 or Δfdh1 Δfdh2 expressing fdh2-His were grown in 1-liter anaerobic Schott bottles (Chemglass) containing McCas-formate medium with a N2:CO2 (80:20 ...
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No products found
because this supplier's products are not listed.
Robert LeDesma, et al.,
bioRxiv - Microbiology 2022
Quote:
... or H249A-HA-tag were seeded onto separate glass coverslips (#1.5; 10 mm; Thomas Scientific, Swedesboro, NJ) in a 24-well plate at 100,000 cells per well ...
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No products found
because this supplier's products are not listed.
Keiko Masuda, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
Non-labeled quantification tags were synthesized by Fluorenylmethyloxycarbonyl (Fmoc) chemistry using a peptide synthesizer SyroWave (Biotage, Uppsala, Sweden). Fmoc amino acids were purchased from Watanabe Chemical Industries and contained the following side chain protecting groups ...
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No products found
Larissa Krüger, et al.,
bioRxiv - Microbiology 2023
Quote:
... and the tag was removed with the TEV protease (ratio 10:1 w/w) during overnight dialysis (Biodesign™ Cellulose Dialysis Tubing Roll ...
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FLAG-tag Antibody is a Rabbit Polyclonal against FLAG-tag.
Cat# abx330268-100UG,
100 µg USD $290.0
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Isabel Brandão, et al.,
bioRxiv - Physiology 2023
Quote:
... or after the primary antibody was incubated with recombinant Human Peroxidasin Homolog Protein (Abbexa; catalog # abx068474), in equimolar (1:1 ...
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No products found
because this supplier's products are not listed.
Raveen Rathnasinghe, et al.,
bioRxiv - Microbiology 2021
Quote:
... were maintained in Dulbecco’s Modified Eagle Medium (DMEM) complemented with 10% heat-inactivated Fetal Bovine Serum (HI-FBS; PEAK serum), penicillin-streptomycin (Gibco ...
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TLR3 suppressor
Sold for research purposes only.
Cat# 2318.0, SKU# 2318-5 mg,
5mg, US $104.50 / EA, EURO, €95 / EA
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Daniel J. Steinberg, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 2.5µg/ml human recombinant Insulin (Biological Industries; 41-975-100) and 3µM CHIR-99021 (Axon Medchem ; 1386) sterilized through 0.22μm filter ...
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No products found
because this supplier's products are not listed.
Amanda L. Photenhauer, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Custom α-Sas6T antiserum was generated by rabbit immunization with purified recombinant Sas6T protein (Lampire Biological Laboratories). The resulting antiserum was used for western blotting and cell staining ...
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No products found
because this supplier's products are not listed.
Patricia Aguilar-Calvo, et al.,
bioRxiv - Pathology 2023
Quote:
... full length recombinant mouse PrP (23-230) generated in E.coli was first conjugated to deferoxamine-maleimide (Macrocyclics) to produce deferoxamine-conjugated PrPC (DFO-PrPC) ...
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No products found
because this supplier's products are not listed.
Nur F. Isa, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... and mutated derivatives of pcDNA3.1-Flag-ICP22 in medium supplemented with p-benzoylphenylalanine (Bpa) (IRIS Biotech) at a final concentration of 2 mM ...
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No products found
because this supplier's products are not listed.
Nozomi Ugajin, et al.,
bioRxiv - Molecular Biology 2023
Quote:
RNA samples were extracted from zebrafish embryos or FLAG-immunoprecipitants using TRI Reagent (Molecular Research Center). Ethachinmate (Nippon gene ...
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No products found
because this supplier's products are not listed.
Benjamin N. Bell, et al.,
bioRxiv - Immunology 2021
Quote:
... by incubating the yeast with a 1:1000 dilution of Chicken anti C-MYC Epitope Tag Primary Antibody from Exalpha Biologicals Inc ...
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No products found
because this supplier's products are not listed.
Nirupa Nagaratnam, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The supernatant containing detergent solubilized His-tagged FopA was filtered through a 0.22 μm cellulose acetate membrane filter (Foxx Life Sciences) and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP ...
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No products found
because this supplier's products are not listed.
Takao Fujisawa, et al.,
bioRxiv - Cell Biology 2023
Quote:
Recombinant proteins or ZnCl2 solution for normalization was incubated with 10 µM ZnAF-2 (Goryo Chemical, SK2001-01) in TBS for 30 min at room temperature ...
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No products found
because this supplier's products are not listed.
Siyi Gu, et al.,
bioRxiv - Cell Biology 2023
Quote:
HEK293 cells expressing Flag-SNAP-CCR5 were seeded onto 10-mm glass-bottom dishes (FluoroDish, FD3510, WPI) previously coated with 5 μg/ml fibronectin ...
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No products found
because this supplier's products are not listed.
Sabina Marciano, et al.,
bioRxiv - Neuroscience 2021
Quote:
... primary antibody incubation was performed for 24 h at room temperature (VMAT2: 1:5000, 20042, Immunostar, Hudson, USA, HA-tag: 1:5000, MMS- 101R, Nordic BioSite, Taby ...
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No products found
because this supplier's products are not listed.
Mu-Sen Liu, et al.,
bioRxiv - Biochemistry 2019
Quote:
... The His6-MBP-Cas9 fusion protein was cleaved to remove the His6-MBP tag by adding the TEV protease to the dialysis tubing (Spectrum labs) during dialysis against buffer containing 20 mM HEPES pH 7.5 ...
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No products found
because this supplier's products are not listed.
Arjen J. Jakobi, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... For the gold labeling experiments the His6-tag was not removed to allow binding of 5 nm Ni-NTA-Nanogold® (Nanoprobes). For consistency the His6-tag was also kept on the proteins for the co-pelletation assay.
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No products found
because this supplier's products are not listed.
Gerda E. Breitwieser, et al.,
bioRxiv - Neuroscience 2023
Quote:
WT GPR37L1 and variants (without or with the Glu-Glu tag) were transfected into GPR37L1-KO SK cells (SK-N-MC Transfection Kit, Altogen Biosystems). Cells were lysed after 48 hours ...
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No products found
because this supplier's products are not listed.
Cassidy M.R. Blackburn, et al.,
bioRxiv - Immunology 2020
Quote:
... media was removed and replaced with either D-10 (untreated) or D-10 + 50μg/ml oxidized LDL (hi oxLDL Kalen biomedical 770252-60) for 2 hours ...
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No products found
because this supplier's products are not listed.
Jasmine Alexander-Floyd, et al.,
bioRxiv - Immunology 2021
Quote:
... supernatants and recombinant cytokine standards were applied to anti-IL-1β antibody-coated (eBioscience) Immulon ELISA plates (ImmunoChemistry Technologies). IL-1β was detected using biotinylated anti IL-1β (eBioscience ...
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No products found
because this supplier's products are not listed.
Glory Nasseri, et al.,
bioRxiv - Neuroscience 2021
Quote:
... resuspended proteins were PEGylated for 1 hr at RT to label newly exposed cysteine thiols with the 5 kDa mass tag reagent (mPEG-5k, Badrilla SiteCounter™). Approximately 20 μl of each supernatant was saved as the “total input.” For immunoblot analysis ...
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No products found
because this supplier's products are not listed.
Vadim Malis, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and their subtraction provides flow-out signals from the tag pulse (Miyazaki et al., 2008; 2011; 2012; 2015; Wheaton et al., 2012). Both tag-on and tag-off acquisitions have the non-sel IR pulse and thus the background signals with exponential T1 relaxation magnetization returns are the same in both images ...
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No products found
because this supplier's products are not listed.
June-Hyung Kim, et al.,
bioRxiv - Immunology 2019
Quote:
... In vitro ubiquitination of Asc was performed by incubating 300 ng of mouse recombinant Asc (CSB-EP861664MO, CUSABIO, Tx, USA), 1 µg of recombinant Mul1 ...
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No products found
because this supplier's products are not listed.
Jialiang S. Wang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Samples (60 µl well−1) were then added along with a standard curve of murine recombinant Sclerostin (Alpco, Salem, NH) and plates were incubated overnight at 4 °C ...
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No products found
because this supplier's products are not listed.
Livia Mazzini, et al.,
bioRxiv - Immunology 2020
Quote:
ELISA plates were coated with 1µg/mL of purified recombinant Spike S1 Protein (aa 18-676) (eEnzyme, Gaithersburg, MD, USA) or with 1µg/mL Spike-RBD (Arg319-Phe541 ...
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No products found
because this supplier's products are not listed.
Maria N Barrachina, et al.,
bioRxiv - Cell Biology 2023
Quote:
... HSPCs were incubated in complete medium containing TPO (50 ng/mL) and recombinant hirudin (100 U/mL, Aniara Diagnostics, RE120A)) for 4 days.42 To assess proplatelet production ...
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No products found
because this supplier's products are not listed.
Haiyan Jia, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... Small RNA-seq library construction was performed using the Perkin Elmer’s Bio Scientific NEXTflex Small RNA-Seq Kit v3 and bar-coded with individual tags following the manufacturer’s instructions (Bioo Scientific Corp, Austin, TX). Libraries were prepared on a Perkin Elmer’s Sciclone G3 NGS Workstation Liquid Handling System ...
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No products found
because this supplier's products are not listed.
Hana Saoud, et al.,
bioRxiv - Neuroscience 2021
Quote:
... It was possible to identify neonates by means of their three-digit metal ear tags (ref. 52-4716, Harvard Apparatus, Les Ulis, France). After surgery ...
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No products found
because this supplier's products are not listed.
Megan N. Thomas, et al.,
bioRxiv - Microbiology 2023
Quote:
Serum samples collected from the indirect contact pigs at 11 DPC were prepared for the hemagglutination inhibition (HI) assay by receptor-destroying enzyme (RDE II; Hardy Diagnostics, Santa Maria, CA) treatment ...
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No products found
because this supplier's products are not listed.
Trung The Tran, et al.,
bioRxiv - Immunology 2022
Quote:
... Purified recombinant viral proteins were solubilized in PBS and biotinylated chemically with sulfo-NHS-LC-biotin (sulfo-NHS-LC-biotin, Proteochem, USA) at a molar ratio of 1:1 ...
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No products found
because this supplier's products are not listed.
David A. Lomas, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
Crystallisation of recombinant Cys232Ser α1-antitrypsin was carried out in 2-well MRC crystallisation plates with a Mosquito robot (TTP Labtech) using 100nl protein solution and 100nl well solution ...
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No products found
because this supplier's products are not listed.
Sandro Roier, et al.,
bioRxiv - Immunology 2023
Quote:
... The protein vaccines were alum-adjuvanted by adsorption of the recombinant fusion proteins to aluminum hydroxide (Alhydrogel®; Croda, Frederikssund, Denmark) as previously described22 ...
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No products found
because this supplier's products are not listed.
Delphyne Descamps, et al.,
bioRxiv - Microbiology 2021
Quote:
... Cell lysates were incubated for 4 h at 4°C with an anti-Flag antibody coupled to agarose beads (Euromedex). The beads were then washed 3 times with lysis buffer and 1 time with PBS ...
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No products found
because this supplier's products are not listed.
Lorraine De Jesús-Kim, et al.,
bioRxiv - Cell Biology 2020
Quote:
For Mcm2-72SORT549/Cdt1 (LPETGG at the C-terminus of Mcm2) the FLAG eluate was attached with Sortase to the peptide NH2-GGGHHHHHHHHHHC-COOH coupled to maleimide-Dy649P1 (Dyomics) as described in Ticau et al ...
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No products found
because this supplier's products are not listed.
Erica Misner, Min Zhang, Eva Sapi,
bioRxiv - Microbiology 2022
Quote:
... PCR products were analyzed by standard agarose gel electrophoresis in a 1% gel referenced against a Hi-Lo DNA ladder (Bionexus, Inc., Oakland, CA, USA; BN2050). An infected zebrafish sample which was positive for B ...
-
No products found
because this supplier's products are not listed.
Aloysius Wong, et al.,
bioRxiv - Plant Biology 2019
Quote:
The UV-visible spectra of affinity purified recombinant DGK4 (200 µg/mL) was recorded on a PHERAstar FS micro-plate reader (BMG Labtech, USA). The heme environment of DGK4 was characterized by the addition of a reducing agent ...
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No products found
because this supplier's products are not listed.
Jack M. Botting, et al.,
bioRxiv - Microbiology 2022
Quote:
... and introduction of the flaG-2313 allele in the chromosome of a kanamycin-sensitive isolate was confirmed by PCR and DNA sequencing (Eton Bioscience). The resulting strain was designated JMB73.
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No products found
because this supplier's products are not listed.
Sandrine Valade, et al.,
bioRxiv - Immunology 2021
Quote:
... ADAMTS13 activity (N: 50-150 IU/dL) was measured with in-house FRETS-VWF73 assay using the recombinant VWF73 peptide (Peptide Institute, Osaka, Japan), as previously described [29] ...
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No products found
because this supplier's products are not listed.
Lennard L. Bohlender, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 10 μg of total protein were mixed with one unit of α-L-arabinofuranosidase from either Aspergillus niger or a corresponding recombinant version (E-AFASE or E-ABFCJ, Megazyme, Bray, Ireland) and incubated over night at 40°C ...
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No products found
because this supplier's products are not listed.
Thamiya Vasanthakumar, et al.,
bioRxiv - Biochemistry 2021
Quote:
Yeast strain SABY31 (VMA1-3×FLAG, stv1Δ)25 in YPD media (11 L) was grown in a Microferm fermenter (New Brunswick Scientific) for 36 hours (OD660=6 to 9 ...
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No products found
because this supplier's products are not listed.
Matthew L. Fabian, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Aliquots of 1 μL of TRV1 and recombinant TRV2 were electroporated into 50 μL tubes of Agrobacterium tumifasciens strain GV3101 cells (Intact Genomics, St. Louis MO, USA) using a Bio-Rad Gene Pulser II and Pulse Controller electroporation system set to 2.2 kV ...