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No products found
because this supplier's products are not listed.
James Brett Case, et al.,
bioRxiv - Microbiology 2020
Quote:
... Secreted hACE2-Fc and mACE2-Fc proteins were purified by protein A chromatography (Goldbio).
-
No products found
because this supplier's products are not listed.
Andrea Di Francesco, et al.,
bioRxiv - Genetics 2023
Quote:
... Antibodies including Fc block (clone 2.42, Leinco Technologies) were added and incubated for 30 minutes at 4°C ...
-
No products found
because this supplier's products are not listed.
Ge Song, et al.,
bioRxiv - Immunology 2020
Quote:
... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
-
No products found
because this supplier's products are not listed.
Gunsagar S. Gulati, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Cells were Fc-blocked with of rat IgG (LifeSpan BioSciences) and stained with 5 μg/ml of rat anti-mouse antibodies (catalog no. ...
-
Cat# HY-P70434-50 μg,
50 μg, USD $180.0
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Yang Meng, et al.,
bioRxiv - Microbiology 2022
Quote:
... 100 μg/ml or 200 μg/ml 5-FC (MedChemExpress), 0.5 μg/ml or 1.0 μg/ml Amp B (MedChemExpress ...
-
No products found
because this supplier's products are not listed.
Kaitavjeet Chowdhary, et al.,
bioRxiv - Immunology 2023
Quote:
... Cells were sorted into DMEM + 5% FCS in DNA Lo-Bind tubes (Eppendorf, cat # 022431021). After spinning down for 5 min at 500g in a refrigerated centrifuge at 4°C ...
-
No products found
because this supplier's products are not listed.
Darya V. Voronina, et al.,
bioRxiv - Immunology 2022
Quote:
... [48] fused with human IgG1 Fc-fragment and Llama IgG2b hinge were purchased from Evrogen (Russia). CHO-S cells were transfected with VHH-Fc containing construct using the CHO Gro System (Mirus Bio ...
-
No products found
because this supplier's products are not listed.
Nathalie Lecat-Guillet, et al.,
bioRxiv - Biophysics 2022
Quote:
The pcDNA plasmid encoding human mGlu2 with N-terminal FLAG- and SNAP-tags was a gift from Cisbio Bioassays (Perking Elmer ...
-
No products found
because this supplier's products are not listed.
Sarah N. Zvornicanin, et al.,
bioRxiv - Biochemistry 2023
Quote:
HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
-
No products found
because this supplier's products are not listed.
Yann Aquino, et al.,
bioRxiv - Genomics 2022
Quote:
... Recombinant IFNα17/αI (PBL Assay Science) was used as calibrator ...
-
No products found
because this supplier's products are not listed.
Cathal Meehan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Recombinant IL-6 with an N-terminal his tag (Fitzgerald, Biosynth Ltd.) was immobilized on His-Pur™ Ni-NTA Resin (ThermoScientific ...
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This membrane protein is Human CD3 epsilon & CD3 delta Heterodimer (23-126(CD3E)&22-105(CD3D))....
Cat# MP1256F,
1.0 case, Inquiry
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Sumit Sen Santara, et al.,
bioRxiv - Immunology 2021
Quote:
... Siglec6-His tag (Creative Biolabs), 4μ8C ...
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No products found
because this supplier's products are not listed.
Jing Zhao, et al.,
bioRxiv - Plant Biology 2023
Quote:
... His-tag antibody (Tiangen, Beijing, China) and GST antibody (Tiangen).
-
No products found
because this supplier's products are not listed.
Sharon R. Stevens, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Flag-tag or DDDDK-tag (MBL International Cat# M185-3L, RRID:AB_11123930); rabbit polyclonal antibodies against AnkR(Ho et al. ...
-
No products found
because this supplier's products are not listed.
Erik Procko,
bioRxiv - Biochemistry 2020
Quote:
Hydrated anti-human IgG Fc biosensors (Molecular Devices) were dipped in expression medium containing RBD-IgG1 for 60 s ...
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No products found
because this supplier's products are not listed.
Kate E. Cavanaugh, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and CO2 control (Chamlide TC and FC-5N; Quorum Technologies). All pieces of the stage incubator (stage ...
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No products found
because this supplier's products are not listed.
Adam K. Wade-Vallance, et al.,
bioRxiv - Immunology 2022
Quote:
... αIgE with a mutated Fc-receptor binding domain (clone R1E4; Cedarlane), or control rat gamma globulin were diluted in D-PBS to a concentration of 0.3mg/mL and injected intravenously to achieve a final dose of 3.25mg/kg ...
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No products found
because this supplier's products are not listed.
Jun-Gu Kang, et al.,
bioRxiv - Microbiology 2019
Quote:
... His-tagged Gn and Gc recombinant proteins were purchased from Immune Technology Co ...
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No products found
because this supplier's products are not listed.
Marcel Lagedroste, et al.,
bioRxiv - Biochemistry 2019
Quote:
Fos-choline 16 (FC-16) and CYMAL5 (C5) were obtained from Anatrace. Lyophilized nisin powder (2.5% nisin content ...
-
No products found
because this supplier's products are not listed.
Julius Brandenburg, et al.,
bioRxiv - Immunology 2020
Quote:
... 10% of heat-inactivated fetal calf serum (FCS; Pan-Biotek, Aidenbach, Germany), supplemented with 50 ng/ml macrophage colony stimulating factor (M-CSF ...
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No products found
because this supplier's products are not listed.
Jiachen Huang, et al.,
bioRxiv - Immunology 2021
Quote:
... 25 µl of secondary antibody (goat anti-human IgG Fc; Meridian Life Science) at a 1:4,000 dilution in blocking buffer was applied to each well for 1 hr at room temperature ...
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No products found
because this supplier's products are not listed.
Yizhi Yuan, et al.,
bioRxiv - Biochemistry 2022
Quote:
... supplemented with 10% (v/v) FCS on 6 cm cell culture dishes (Sarstedt). After 48 h ...
-
No products found
because this supplier's products are not listed.
Shan Jiang, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The protein standard with the C-terminal FLAG tag (E-PKSH032870.10) was from Biomol. Linear DNA templates were produced by PCR and were PCR purified ...
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No products found
because this supplier's products are not listed.
Hertzog Maud, et al.,
bioRxiv - Microbiology 2022
Quote:
... The short fluorescent ssDNA substrate used in FCS experiments was prepared with synthetic oligonucleotides (Eurogentec) labeled either with Biotin or Alexa-488 in 5’ in order to generate a Biotin-labeled DNA strand and a fluorescently-labeled DNA strand (Sequence ...
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No products found
because this supplier's products are not listed.
Yang Liu, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Lin- c-Kit+ cells were sorted into 2%FCS-PBS with 100µM IWR-1 (Tocris, 3532) or control vehicle ...
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No products found
because this supplier's products are not listed.
Lianghui Zhang, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... and levels of the IgG1 Fc moiety were measured by Human IgG ELISA Kit (Immunology Consultants Laboratory). To characterize different routes side-by-side ...
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Recombinant protein produced in Pichia pastoris. Free of animal derived components, RNases, and...
Cat# LS006361,
10 ku, $200.00
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Kyle T. Mincham, et al.,
bioRxiv - Immunology 2023
Quote:
... minced tissues were resuspended in GKN + 10% fetal calf serum (FCS) with collagenase IV (Worthington Biochemical Corporation) and DNase (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Herman KH Fung, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Confocal volume estimation was carried out by ten 1-min FCS measurements of 10 nM Atto488 (ATTO-TEC) in water ...
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No products found
because this supplier's products are not listed.
Darya V. Voronina, et al.,
bioRxiv - Immunology 2022
Quote:
... CHO-S cells were transfected with VHH-Fc containing construct using the CHO Gro System (Mirus Bio, WI, USA), according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Aleksandra Luginina, et al.,
bioRxiv - Biophysics 2023
Quote:
... The final gene construct was completed from the 3’-terminus by KpnI restriction site followed by 9×His-tag nucleotide sequence and inserted into the integrative inducible expression vector pLEXSY_I-blecherry3 (Jena Bioscience, Germany) via the BglII(5’ ...
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No products found
because this supplier's products are not listed.
Jia Chen, et al.,
bioRxiv - Microbiology 2019
Quote:
... The blots were washed with PBS and incubated with primary antibodies (anti-EBV gH/gL (a rabbit polyclonal antiserum;1:200) (45) and anti-His tag antibody (OB05, Calbiochem, 1:1000) for His-tagged KSHV gL ...
-
No products found
because this supplier's products are not listed.
Jason S Presnell, William E Browne,
bioRxiv - Developmental Biology 2021
Quote:
... Recombinant Cas9 protein (PNA Bio) and sgRNAs were injected at concentrations of 400 ng/µl of Cas9 protein and 100 ng/µl for each sgRNA ...
-
No products found
because this supplier's products are not listed.
Evangelos Stefanidis, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Binding of T cell-secreted A4-Fc on OT-I CD47 was assessed by staining with labeled goat anti-mouse IgG Fc (polyclonal, antibodies-online) at 4°C for 30min ...
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No products found
because this supplier's products are not listed.
Djem U. Kissiov, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... In all cases medium contained 5% FCS (Omega Scientific), 0.2 mg/mL glutamine (Sigma) ...
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No products found
because this supplier's products are not listed.
Philippe C Després, et al.,
bioRxiv - Evolutionary Biology 2024
Quote:
... Cytosine and 5-FC were purchased from Alfa Aesar (now Thermo Scientific Chemicals ...
-
No products found
because this supplier's products are not listed.
Cassian Afting, et al.,
bioRxiv - Bioengineering 2024
Quote:
WNT surrogate-Fc Fusion Protein (Wnt-surrogate; ImmunoPrecise Antibodies Ltd. ...
-
No products found
because this supplier's products are not listed.
Joanna M. Cooper, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and IV Fc chimera proteins were produced by Molecular Innovations. His-tagged recombinant human tau variants 2N4R ...
-
No products found
because this supplier's products are not listed.
Aleksei Kuznetsov, et al.,
bioRxiv - Biochemistry 2020
Quote:
Human recombinant ACE2-His protein (Icosagen OÜ, Estonia, cat# P-302-100) and SARS-CoV-2 Spike protein S1 (Icosagen OÜ ...
-
No products found
because this supplier's products are not listed.
Madeleine F. Jennewein, et al.,
bioRxiv - Immunology 2021
Quote:
... pαH-MERS RBD-Fc using 2 mg of polyethyleneimine (Polyscience, Cat# 24765). After 6 days of growth ...
-
No products found
because this supplier's products are not listed.
Mirjam C van der Net, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... was recloned in a pDONR221 backbone and the Myc-tag was replaced with a Flag-tag (Epoch Life Science). The sequence 5’-CAATGCGGAATATCAATCCCAGCACAGCAAATTCTCCA AAATGTCAGG-3’ was added between base pairs 982 and 983 of the YAP1 gene ...
-
No products found
because this supplier's products are not listed.
Uri Elia, et al.,
bioRxiv - Immunology 2020
Quote:
... pseudouridine-substituted Fc-conjugated RBD mRNA (331-524 aa) was purchased from TriLink Bio Technologies (San Diego ...
-
No products found
because this supplier's products are not listed.
Mohammad Saeed, et al.,
bioRxiv - Immunology 2022
Quote:
... Fetal calf serum (FCS) and antibiotics were obtained from American Type Culture Collection (ATCC). Trypan blue (Life technologies ...
-
No products found
because this supplier's products are not listed.
Albert S. W. Kang, et al.,
bioRxiv - Biophysics 2020
Quote:
... Purification from excess OsBp was done with spin columns (TC-100 FC from TrimGen Corporation) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Max A. B. Haase, et al.,
bioRxiv - Genetics 2022
Quote:
Recombinant His6-tag DASH complex was linked to 0.56 μm-diameter streptavidin-coated polystyrene microbeads (Spherotech) using biotinylated His5-antibody (Qiagen) ...
-
No products found
because this supplier's products are not listed.
Liu He, et al.,
bioRxiv - Biochemistry 2022
Quote:
... coli strain BL21 (DE3) with an N-terminal (His)6-SUMO tag from a pE-SUMO-pro expression vector (LifeSensors). Cells were grown in 2YT media at 24°C to an OD600 of 0.8 and then induced with 0.1 mM isopropyl β-D-thiogalactopyranoside (IPTG ...
-
No products found
because this supplier's products are not listed.
Karen. M. Marshall, et al.,
bioRxiv - Bioengineering 2023
Quote:
... the scaffolds seeded with C2C12 cells were moved to a 24 well plate and 1.5 mL of media was added (basal with 2% FCS ± 5 µg of BMP-2 (3.33 µg/mL BMP-2). Culture was continued for 4 days and ALP staining performed on the 5th day from cell seeding ...
-
No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The levels of S protein in different brain regions upon its intranasal infusion were measured using His-Tag protein ELISA kit that allows detection and quantification of poly-histidine-tagged proteins in a biological sample (Cell Biolabs Inc., CA, USA). The experimental procedure was performed as recommended by the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Roberto Espinoza-Corral, et al.,
bioRxiv - Plant Biology 2023
Quote:
... 150 mM NaCl and 0.01% tween-20) overnight at 4°C (anti-PsbA; AS05 084A; anti-PsaB; AS10 695; anti-APC; AS08 277; Agrisera, anti-His tag; TA150087; OriGene). The membrane was washed 3 times in TBST-T at room temperature for 15 minutes each wash followed by incubation with secondary polyclonal anti-rabbit antisera HRP for one hour at room temperature in TBS-T (Jackson ImmunoResearch ...
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No products found
because this supplier's products are not listed.
Tian Yuan, et al.,
bioRxiv - Neuroscience 2023
Quote:
... anti-Flag (Abbkine), anti-β-Actin (Biodragon) ...
-
No products found
because this supplier's products are not listed.
Satyaveni Malasala, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and His-DBS (DH/PH domain) were purchased from Cytoskeleton, Inc ...