-
No products found
because this supplier's products are not listed.
Sofia Rossini, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... while recombinant human IDO1 protein was obtained by Giotto Biotech. Construct expressing murine Src was obtained from Origene.
-
No products found
because this supplier's products are not listed.
Nirupa Nagaratnam, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The supernatant containing detergent solubilized His-tagged FopA was filtered through a 0.22 μm cellulose acetate membrane filter (Foxx Life Sciences) and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP ...
-
No products found
because this supplier's products are not listed.
Koichi Sato, Aiko G.M. Hendrikx, Puck Knipscheer,
bioRxiv - Biochemistry 2023
Quote:
... The wild-type protein was overexpressed as a N-terminal His6-tagged protein in the E.coli JM109(DE3) cells (Intact Genomics) cultured in 4.4 L LB medium and purified by the previously described method53 ...
-
No products found
because this supplier's products are not listed.
Sunada Khadka, et al.,
bioRxiv - Biochemistry 2022
Quote:
... hygromycin resistance marker and red fluorescent protein (RFP) were purchased from Cellecta. For transfection ...
-
No products found
because this supplier's products are not listed.
Sandro Roier, et al.,
bioRxiv - Immunology 2023
Quote:
... The protein vaccines were alum-adjuvanted by adsorption of the recombinant fusion proteins to aluminum hydroxide (Alhydrogel®; Croda, Frederikssund, Denmark) as previously described22 ...
-
Green Fluorescent Protein (GFP) is a recombinant non-glycosylated jellyfish protein expressed in E. coli.
Cat# abx260632-10UG,
10 µg USD $261.0
Ask
Isabel Brandão, et al.,
bioRxiv - Physiology 2023
Quote:
... or after the primary antibody was incubated with recombinant Human Peroxidasin Homolog Protein (Abbexa; catalog # abx068474), in equimolar (1:1 ...
-
No products found
because this supplier's products are not listed.
Eli Fritz McDonald, et al.,
bioRxiv - Cell Biology 2021
Quote:
... purified from B lymphocyte hybridoma cells using a Recombinant Protein G - Sepharose 4B Affinity Column on an ÄKTA start protein purification system (GE Life Science Product # 29022094). Antibodies were used in immunoblotting buffer (5% bovine serum albumin (BSA ...
-
No products found
because this supplier's products are not listed.
Md. Abu Sayeed, et al.,
bioRxiv - Immunology 2022
Quote:
The purified recombinant ICP1 bacteriophage proteins were used to raise mAbs via a commercial vendor (ProMab Biotechnologies, Inc.) using a conventional hybridoma technique (28) ...
-
No products found
because this supplier's products are not listed.
Takao Fujisawa, et al.,
bioRxiv - Cell Biology 2023
Quote:
Recombinant proteins or ZnCl2 solution for normalization was incubated with 10 µM ZnAF-2 (Goryo Chemical, SK2001-01) in TBS for 30 min at room temperature ...
-
No products found
because this supplier's products are not listed.
Sihan Chen, et al.,
bioRxiv - Bioengineering 2024
Quote:
Green fluorescent protein (GFP) labeled endothelial cells were cultured in endothelial cell culture medium (ECM) (ScienCell, CA, USA) with 5% (v/v ...
-
No products found
because this supplier's products are not listed.
Sahiti Kuppa, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 180 μl of either fluorescent protein was added to a 3 mm path length quartz cuvette (Starna Cells Inc.) and maintained at 23 °C in a PC1 spectrofluorometer (ISS Inc.) ...
-
No products found
because this supplier's products are not listed.
Livia Mazzini, et al.,
bioRxiv - Immunology 2020
Quote:
ELISA plates were coated with 1µg/mL of purified recombinant Spike S1 Protein (aa 18-676) (eEnzyme, Gaithersburg, MD, USA) or with 1µg/mL Spike-RBD (Arg319-Phe541 ...
-
No products found
because this supplier's products are not listed.
Ália dos Santos, et al.,
bioRxiv - Biophysics 2021
Quote:
... expression of recombinant proteins was set up by infecting sf21 cells with the P3 viral stock in Spodopan media (PAN Biotech). To ensure correct folding of the MVI constructs ...
-
No products found
because this supplier's products are not listed.
Trung The Tran, et al.,
bioRxiv - Immunology 2022
Quote:
... Purified recombinant viral proteins were solubilized in PBS and biotinylated chemically with sulfo-NHS-LC-biotin (sulfo-NHS-LC-biotin, Proteochem, USA) at a molar ratio of 1:1 ...
-
No products found
because this supplier's products are not listed.
Stacia M. Nicholson, Francis A.X. Schanne,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
Recombinant mouse TNFSF11 (IBI Scientific, Indiana), also known as RANKL ...
-
No products found
because this supplier's products are not listed.
Rachel E. Hardy, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... far-red fluorescent dye DRAQ7 (BioStatus) added to detect late-stage apoptotic or necrotic cells ...
-
No products found
because this supplier's products are not listed.
Farwa Sajadi, Jean-Paul Paluzzi,
bioRxiv - Physiology 2024
Quote:
... AedaeItp-l or control Egfp (enhanced green fluorescent protein) dsRNA using a Nanoject III Programmable Nanoliter Injector (Drummond Scientific, Broomall, PA, USA).
-
No products found
because this supplier's products are not listed.
Alberto Domingo López-Muñoz, et al.,
bioRxiv - Microbiology 2023
Quote:
... alone or in combination with purified recombinant proteins were placed in the lower chamber of a 96-well ChemoTx System plate (Neuro Probe # 101-5) in RPMI 1640 1 % FBS ...
-
No products found
because this supplier's products are not listed.
Shiri Levy, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 25 ng/ml human recombinant FGF4 (Reliatech), 2 ng/ml human recombinant TGF-ß1 (PeproTech) ...
-
No products found
because this supplier's products are not listed.
Ye Qiu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Mouse anti-His tag monoclonal antibody (Bioworld, USA, 1:5000) was used as the first antibody ...
-
No products found
because this supplier's products are not listed.
Ruilian Li, et al.,
bioRxiv - Microbiology 2021
Quote:
... Fluorescent labeling of the laminaripentaose (Megazyme, Bray, Ireland) was accomplished by reacting the respective oligosaccharide glycamines with Lissamine rhodamine B sulfonyl chloride (LRSC ...
-
No products found
because this supplier's products are not listed.
Mathilde Ambrosino, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The concentrations of released fluorescent molecules and cytokines in the supernatants were quantified using either a microplate reader for fluorescent measurements (Tristar 2S, Berthold Technologies) or ELISA kits (R&D Systems ...
-
No products found
because this supplier's products are not listed.
Philip Pickford, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... Exendin-4 fluorescent EIA kits were obtained from Phoenix Pharmaceuticals. All other standard laboratory chemicals or culture reagents were obtained from Sigma or Thermo Fisher unless otherwise specified ...
-
No products found
because this supplier's products are not listed.
Michael S. Balzer, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... and 10 U/ml recombinant mouse interferon-□ (Cell Sciences, Canton, MA) at 33 °C (permissive conditions ...
-
No products found
because this supplier's products are not listed.
Liangxi Wang, et al.,
bioRxiv - Genomics 2022
Quote:
... 10 ng/ml recombinant human TNFα (Cell Applications, cat# RP1111-50) was added for 3 hours ...
-
No products found
because this supplier's products are not listed.
Melisa Lázaro, et al.,
bioRxiv - Microbiology 2020
Quote:
... This was confirmed by labeling N-terminally His6-tagged mL-GDH180 with Ni-NTA-Nanogold (Nanoprobes) and visualizing particles by negative staining electron microscopy ...
-
No products found
because this supplier's products are not listed.
Temitayo T. Bamgbose, et al.,
bioRxiv - Immunology 2023
Quote:
... and treated with recombinant mouse IL-4 (Leinco technologies, Inc. I-207) at a 40ng/ml concentration for 6 hours ...
-
No products found
because this supplier's products are not listed.
Ghulam Destgeer, et al.,
bioRxiv - Bioengineering 2020
Quote:
... particles were incubated with varying concentrations of human recombinant NT-proBNP (HyTest, Finland) for 1 hr with subsequent washing ...
-
No products found
because this supplier's products are not listed.
Nicolas Lamassiaude, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... Recombinant constructs were purified using EZNA Plasmid DNA Mini kit (Omega Bio-Tek) and sequence-checked (Eurofins Genomics) ...
-
No products found
because this supplier's products are not listed.
Xinyu Xie, et al.,
bioRxiv - Immunology 2024
Quote:
... media containing PBS or recombinant IL-1β (10 ng/ml) (Abbkine, PRP100051, USA) was added to the basal chambers for 3 days.
-
No products found
because this supplier's products are not listed.
Aditi Deshpande, et al.,
bioRxiv - Microbiology 2022
Quote:
... library preparation and Hi-Seq 2 x150 paired-end sequencing were performed by GENEWIZ (New Jersey, USA).
-
No products found
because this supplier's products are not listed.
Anna K. Hundsdoerfer, et al.,
bioRxiv - Genomics 2023
Quote:
... euphorbiae (from one head each; the same individuals used for the PacBio genome and Hi-C sequencing) and neural tissue from the internal reference standard Acheta domesticus (female ...
-
No products found
because this supplier's products are not listed.
Colin Lock, Melissa M. Gabriel, Bastian Bentlage,
bioRxiv - Molecular Biology 2023
Quote:
DNA was extracted from each tagged colony using the GenCatch genomic DNA extraction kit (Epoch Life Science, Sugar Land, TX) following the manufacturer’s protocol for tissue samples ...
-
No products found
because this supplier's products are not listed.
Erin J. Vanzyl, et al.,
bioRxiv - Cell Biology 2020
Quote:
... ATF3 mRNA expression was determined using Bioline SensiFAST™ Probe HI-ROX Master Mix (FroggaBio Inc, Toronto, ON) with SYBR green ...
-
No products found
because this supplier's products are not listed.
Neetu Singh Kushwah, et al.,
bioRxiv - Molecular Biology 2021
Quote:
AAE3 protein sequences from Medicago truncatula (GenBank ...
-
No products found
because this supplier's products are not listed.
Celia Fernandez-Sanz, et al.,
bioRxiv - Physiology 2021
Quote:
Equal amounts of protein (70 μg) supplemented with 5x Protein Loading Buffer (National Diagnostics, USA) were preheated (95°C ...
-
No products found
because this supplier's products are not listed.
Helene Jahn, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Protein concentration was determined by SDS-PAGE (Coomassie or Fluorescence Protein gel stain (Lamda Biotech)) in comparison with standards ...
-
No products found
because this supplier's products are not listed.
Xavier Martiáñez-Vendrell, et al.,
bioRxiv - Microbiology 2024
Quote:
... and Cy3-conjugated mouse-α-biotin #200-162-211 (200-162-211, Jackson Immuno Research) were used for fluorescent detection with an Alliance Q9 Advanced (UVITEC, Cambridge, UK) scanner ...
-
No products found
because this supplier's products are not listed.
Raeann Goering, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... with protein ladder (Gel Company FPL-008). Gels were imaged with a Sapphire molecular imager (Azure Biosystems ...
-
No products found
because this supplier's products are not listed.
Guojun Wu, et al.,
bioRxiv - Microbiology 2022
Quote:
... lipopolysaccharide-binding protein (Hycult Biotech, PA, USA), leptin (P&C ...
-
No products found
because this supplier's products are not listed.
Yu-Yi Kuo, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The supernatants were collected and the protein concentration was quantified by the Bradford protein assay (BioShop Canada Inc., Burlington, Ontario, Canada). Protein samples were stored at −80°C for subsequent western blotting assessments.
-
No products found
because this supplier's products are not listed.
Mackenzie E. Ryan, et al.,
bioRxiv - Microbiology 2023
Quote:
... Protein sequence alignments were performed using MegAlign Pro (DNAstar). Superimposed images of protein structures were generated using ChimeraX Matchmaker84 ...
-
No products found
because this supplier's products are not listed.
Shivani Ahuja, et al.,
bioRxiv - Biochemistry 2020
Quote:
... the protein was mixed 2:3 with monoolein (Nu-Chek Prep) and then 70 nl of this material was deposited on a glass slide (Molecular Dimensions) ...
-
No products found
because this supplier's products are not listed.
Gab-Chol Choi, et al.,
bioRxiv - Bioengineering 2020
Quote:
... and bone morphogenetic protein 2 (BMP-2) (1:200, orb251474, Biorbyt) primary antibodies at 4°C ...
-
No products found
because this supplier's products are not listed.
Adam Beck, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The antibodies and protein standards are commercially available from EnCor Biotechnology Inc.
-
No products found
because this supplier's products are not listed.
Grant A. King, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Protein extracts were generated by pulverization using a Mini-Beadbeater-96 (BioSpec). The samples were then treated with 50 µl of 3X SDS sample buffer (187.5 mM Tris pH 6.8 ...
-
No products found
because this supplier's products are not listed.
Arthur Forer, Shotaro Otsuka,
bioRxiv - Cell Biology 2023
Quote:
Gold beads (15 nm) conjugated with Protein A (Cytodiagnostics, Burlington, Ontario, Canada) were absorbed on both sides of the sections as fiducial markers for tomography reconstruction ...
-
No products found
because this supplier's products are not listed.
Lennart Schada von Borzyskowski, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... all protein digests were desalted using C18 microspin columns (Harvard Apparatus, Holliston, USA) according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Bryan D. Ryder, et al.,
bioRxiv - Biochemistry 2020
Quote:
... the protein solution was loaded into 3.5kDa cutoff Biotech CE Dialysis Tubing (Spectrum Labs) and dialyzed overnight at 4°C in 1xPBS to restore native folding ...
-
No products found
because this supplier's products are not listed.
Domagoj Cikes, et al.,
bioRxiv - Genetics 2023
Quote:
... Fifty ug of protein was assayed with 0.2 µCi of [14C]-phosphoethanolamine (P-Etn) (American Radiolabeled Chemical) in 50µl of reaction mixture of 50 mM MgCl2 ...