-
No products found
because this supplier's products are not listed.
Cristiana Cruceanu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Real-time PCR reactions were run in quadruplicate using PrimeTime qPCR Primer Assays (IDT DNA) and PrimeTime® Gene Expression Master Mix (IDT DNA ...
-
No products found
because this supplier's products are not listed.
Veronica Ferrucci, et al.,
bioRxiv - Genetics 2020
Quote:
... Real-time RT-PCR was performed using ‘RealQquality RQ-2019-nCoV’ kits (Ab Analitica; IVD approved) for detection of the RdRp and E genes (from the SarsCov2 N gene) ...
-
No products found
because this supplier's products are not listed.
Romel Menacho-Melgar, Tian Yang, Michael D. Lynch,
bioRxiv - Synthetic Biology 2021
Quote:
... All experiments were performed using a thermocycler (Bulldog Bio, Portsmouth, NH). The experimental design was a definitive screening design and was tabulated using JMP Pro 15 software (SAS Institute ...
-
No products found
because this supplier's products are not listed.
Haley M. Geertsma, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Real-time quantitative PCR was performed using Green-2-Go qPCR Master Mix (Bio Basic cat# QPCR004-S) with 25 ng cDNA per reaction and primers targeting mouse Gapdh (Forward ...
-
No products found
because this supplier's products are not listed.
Yvonne Giesecke, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... Quantitative real-time human-specific primer sets for CyberGreen PCR amplifications were obtained from TIB Molbiol (Berlin, Germany). Primer sequences were:
-
No products found
because this supplier's products are not listed.
Ross D Overacker, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... All cell lines were tested mycoplasma-negative by real-time PCR (MycoSolutions mycoplasma detection kit, Akron Biotech, Boca Raton, FL).
-
No products found
because this supplier's products are not listed.
Paul J. Baker, et al.,
bioRxiv - Immunology 2024
Quote:
... Genotyping was performed by Transnetyx using real-time PCR and genetic background analysis was submitted through Transnetyx and performed by Neogen using the MiniMUGA platform to confirm that all mice were on a C57BL/6 background ...
-
No products found
because this supplier's products are not listed.
Jiling Feng, Yuexun Tang, Wenwei Fu, Hongxi Xu,
bioRxiv - Cell Biology 2023
Quote:
... RT-PCR was performed with a one-step real time PCR using KAPA SYBR FAST One-Step qRT-PCR Universal (D-MARK Biosciences). Primers were used as previously described [18] ...
-
No products found
because this supplier's products are not listed.
Mandeep K. Mann, et al.,
bioRxiv - Biochemistry 2023
Quote:
Substructure search was run against the Enamine REAL database (Enamine Ltd., 2022). Compounds were clustered in ICM (Molsoft ...
-
No products found
because this supplier's products are not listed.
Evan H. Lyall, et al.,
bioRxiv - Neuroscience 2020
Quote:
... but this time over a feedback controlled heater (FHC). Up to 1% isoflurane was provided to maintain anesthetic depth ...
-
No products found
because this supplier's products are not listed.
Zhexin Wang, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and 3 times at 10,000 rpm for 5 s (IKA TC10 basic ULTRA-TURRAX® homogenizer with S10N-5G dispersing element ...
-
No products found
because this supplier's products are not listed.
Michelle E. Maxson, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Time series images were analyzed using Volocity v6.3 software (Quorum Technologies), and SidK-AL568 molecule fluorescence over time plotted to generate traces where the number of steps required for SidK-AL568 spot disappearance were counted ...
-
No products found
because this supplier's products are not listed.
Francesca Billwiller, et al.,
bioRxiv - Neuroscience 2019
Quote:
... amplified 5000 times with a 16 channels amplifier (A-M System) and collected on a computer via a CED interface using Spike 2 software (Cambridge Electronic Design) ...
-
No products found
because this supplier's products are not listed.
Adam S Hassan, et al.,
bioRxiv - Immunology 2019
Quote:
... plates were washed three times with phosphate buffered saline (PBS: Wisent Bioproducts) and replaced in the incubator with DMEM-FBS containing 50 μg/mL gentamicin (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Horacio G. Rotstein, Farzan Nadim,
bioRxiv - Neuroscience 2019
Quote:
... and 3 times following bath application of 1 μM proctolin (Bachem, USA). Impedance was measured using the fast Fourier transform function in MATLAB (MathWorks ...
-
No products found
because this supplier's products are not listed.
Isabelle Matthiesen, et al.,
bioRxiv - Bioengineering 2022
Quote:
... time dialysis (MWCO 12-14 kDa, Spectra/Por RC, Spectrum Laboratories Inc.) was performed in acetonitrile:MilliQ water (1:10 v/v ...
-
No products found
because this supplier's products are not listed.
Lindsay Broadbent, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were passaged three times in Promocell Airway Epithelial Cell Growth Medium (Promocell) then seeded onto collagen-coated Transwell supports (Corning ...
-
No products found
because this supplier's products are not listed.
Germán Michelis, et al.,
bioRxiv - Cell Biology 2021
Quote:
... then washed three times with PBS and reincubated with anti-GAPDH (Genetex GT239) (2017 ...
-
No products found
because this supplier's products are not listed.
Clement Gallay, et al.,
bioRxiv - Microbiology 2021
Quote:
... The resin was then washed 3 times in GFP-Trap_A Wash buffer (Chromotek) and GFP-proteins were eluted using SDS sample buffer at 95°C for 10 min and analyzed by immunoblotting.
-
No products found
because this supplier's products are not listed.
Gillian Dekkers, et al.,
bioRxiv - Immunology 2020
Quote:
... plates were washed 5 times with PBS containing 0.02% Tween-20 (PBS; Sanquin Diagnostics) using an ELISA washer (Biotek ...
-
No products found
because this supplier's products are not listed.
Carolina García-Poyatos, et al.,
bioRxiv - Physiology 2019
Quote:
... washed three times in PBS for 10 min and decalcified in Immunocal (American MasterTech) at room temperature (RT ...
-
No products found
because this supplier's products are not listed.
A. Rossi, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... we performed a likelihood ratio test in DESeq2 to identify the genes that changed subcellular distribution across time (reduced design formula in DESeq2: ∼ days_in_BMP + subcellular_compartment + days_in_BMP:pair_within_day). Z-scores of protein-coding genes (from fracRNA-seq data ...
-
No products found
because this supplier's products are not listed.
Wenyan Fu, et al.,
bioRxiv - Bioengineering 2020
Quote:
... the cells were washed three times with PBS and incubated with Accutase (Innovative Cell Technologies) for 10 min at 37 °C ...
-
No products found
because this supplier's products are not listed.
Jenna E Gallegos, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
Plates were imaged over two-hour time courses using a Phenobooth (Singer Instruments, Somerset, UK) imaging platform ...
-
No products found
because this supplier's products are not listed.
Anthony C. Fredericks, et al.,
bioRxiv - Microbiology 2019
Quote:
... at the time of cell seeding using TransIT-insect transfection reagent (Cambridge Biosciences, Cambridge, UK) as per manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Ahmed Ali, et al.,
bioRxiv - Biophysics 2023
Quote:
Mouse sera were diluted ten times in PEG dilution buffer (PEGD50-1, Life Diagnostics, Inc.), and sequentially in 1:3 dilution steps added to the wells of PEG-BSA coated ELISA plates (PBSA20PL ...
-
No products found
because this supplier's products are not listed.
Anindya Ganguly, et al.,
bioRxiv - Plant Biology 2019
Quote:
... rinsed with sterile water four times and planted on 0.5X Murashige and Skoog medium (Caisson Laboratories). Seeds were stratified at 4 °C for 2 d and then germinated at 20 °C with 16 h of 120-140 µmol light ...
-
No products found
because this supplier's products are not listed.
Christine Müller, et al.,
bioRxiv - Physiology 2020
Quote:
... After different time points the blood glucose concentration was measured using a glucometer (AccuCheck Aviva, Roche). For the determination of insulin sensitivity ...
-
No products found
because this supplier's products are not listed.
Catherine A. Cotter, et al.,
bioRxiv - Microbiology 2022
Quote:
... wells were washed three times with 250 μl PBS + 0.05% Tween-20 (PBS-T, Accurate Chemical). Plates were blocked for 2 h at room temperature with 200 μl PBS-T + 5% nonfat milk and subsequently washed three times with PBS-T prior to incubation with a series of eight 4-fold dilutions of mouse sera for 1 h at room temperature ...
-
No products found
because this supplier's products are not listed.
Anthony J. Snyder, Andrew T. Abad, Pranav Danthi,
bioRxiv - Microbiology 2021
Quote:
... The cells were then washed three times with PBS and lysed with Tri Reagent (Molecular Research Center). Total RNA was extracted from the cell lysates by following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Benoit Besson, et al.,
bioRxiv - Microbiology 2022
Quote:
... Cells were harvested at the indicated time points for total RNA isolation using RNA-Solv (Omega Bio-tek). For replicon assays ...
-
No products found
because this supplier's products are not listed.
M. Koltun, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Each time tissues were homogenized using Bullet Blender with 0.5 mm RNase Free Zirconium Oxide Beads (Next Advance) in RLT buffer from RNeasy Plus Micro Kit (Qiagen) ...
-
No products found
because this supplier's products are not listed.
Tamadher A. Alghamdi, et al.,
bioRxiv - Physiology 2022
Quote:
... after which time some mice were switched to high fat diet (HFD; 60 % fat; Bio-Serv, CA89067-471) for an additional 8 weeks.
-
No products found
Janine L. Brown, et al.,
bioRxiv - Zoology 2019
Quote:
... All plates were then washed four times (1:20 dilution, 20X Wash Buffer Cat. No. X007; Arbor Assays), blotted dry ...
-
No products found
because this supplier's products are not listed.
Tomokazu S. Sumida, et al.,
bioRxiv - Immunology 2022
Quote:
... The lentivirus containing media was harvested 72 h after transfection and concentrated 80 times using Lenti Concentrator (Origene). LV particles were then resuspended in RPMI 1640 media without serum and stored at −80°C before use ...
-
No products found
because this supplier's products are not listed.
Yukiko Harima, et al.,
bioRxiv - Neuroscience 2024
Quote:
... The sections were washed three times with PBS and mounted with cover glass using Fluoromount (#K024; Diagnostic BioSystems).
-
No products found
Khyati Dalal, et al.,
bioRxiv - Genomics 2024
Quote:
... for 20 min and washed three times with PBS before mounting them on a glass bottom plate (Cellvis) coated with poly-L-lysine (Sigma) ...
-
No products found
because this supplier's products are not listed.
Shuangshuang Gu, et al.,
bioRxiv - Immunology 2021
Quote:
Serum samples were collected at indicated time points to detect anti-dsDNA by ELISA (Alpha Diagnostic International, Cat#5110) and Hep-2 cells slides (Inova diagnostics Cat#708750 ...
-
No products found
because this supplier's products are not listed.
Romain Gasser, et al.,
bioRxiv - Microbiology 2020
Quote:
... Wells were washed four times and bound antibodies were detected by the addition of 100 μL of 3,3′,5,5′-tetramethylbenzimidine (ScyTek Laboratories). The enzymatic reaction was stopped by the addition of 100 μL 1 N H2SO4 and the absorbance was measured at 450/630 nm within 5 minutes.
-
No products found
because this supplier's products are not listed.
Pamela Duran, et al.,
bioRxiv - Physiology 2022
Quote:
... rinsed three times in 1xPBS and incubated in blocking buffer (10% normal goat serum (Gemini Bio-products, Sacramento, CA) + 0.3% Triton X-100 (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Ashley Priddey, et al.,
bioRxiv - Immunology 2023
Quote:
... The liquid phase was aspirated and beads were washed with 10mM PBS/0.05% Tween-20 three times before incubation with PE-conjugated anti-human IgG-Fc antibody (Leinco/Biotrend) for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Yun-Wei Lien, et al.,
bioRxiv - Microbiology 2024
Quote:
... 4 μL of the cell mixture was applied three times onto glow-discharged copper EM grids (R2/2, Quantifoil), followed by plunge freezing into liquid ethane/propane ...
-
No products found
because this supplier's products are not listed.
Eliana Battistella, et al.,
bioRxiv - Biophysics 2022
Quote:
... Specimens were then washed three times with 1X PBS to remove excess antibody and incubated in CUBIC-R+ (TCI chemicals) for 3 days for refractive index matching.
-
No products found
because this supplier's products are not listed.
Kristian Spilling, et al.,
bioRxiv - Plant Biology 2019
Quote:
... Nanophytoplankton were distinguished from picoplankton based on light scatter (FSC and SSC) and pulse width (transit time) compared to 3 µm UltraRainbow fluorescent particles (Spherotech).
-
No products found
Maximilian W. G. Schneider, et al.,
bioRxiv - Cell Biology 2021
Quote:
... RO3306 was removed and cells were washed three times with imaging medium containing 1.5 μM okadaic acid (LC laboratories, O-5857).
-
No products found
because this supplier's products are not listed.
Krithika Rao, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cells were washed in PBS (3 times for 5 mins) prior to nuclear counterstaining with DAPI in mounting media (Vector BioLabs).
-
No products found
because this supplier's products are not listed.
Bo-wen Dong, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... The cells were washed three times with PBS and lysed in RIPA buffer containing protease phosphatase inhibitors (Boster biological, Wuhan, China) for 30 min on ice under shaking ...
-
No products found
Johan Panek, et al.,
bioRxiv - Microbiology 2023
Quote:
... After 30 min the cells were washed 2 times with medium and fresh medium with or without treatment was added (torin-1 31-250 nM Selleck Chemicals, rapamycin 12.5-250 nM Selleck Chemicals ...
-
No products found
because this supplier's products are not listed.
Angelo Ascani, et al.,
bioRxiv - Immunology 2023
Quote:
... a 4.5 mm continuously releasing pellet containing 25mg of flutamide (releasing time 90 days, Innovative Research of America, cat. number NA-152), an androgen receptor antagonist ...
-
No products found
because this supplier's products are not listed.
Wenjuan Wang, et al.,
bioRxiv - Pathology 2024
Quote:
... cells were treated with or without CCCP (10 µM) for different times and subsequently cultured in fresh growth medium containing 500 µM biotin-phenol (AdipoGen Life Sciences) at 37°C under 5% CO2 for 30 minutes ...