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No products found
because this supplier's products are not listed.
Oluwaseun M. Ajayi, et al.,
bioRxiv - Physiology 2021
Quote:
RNA was extracted from the accessory glands by homogenization (BeadBlaster 24, Benchmark Scientific) in Trizol reagent (Invitrogen) ...
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No products found
because this supplier's products are not listed.
Chathura Wijesinghege, et al.,
bioRxiv - Plant Biology 2022
Quote:
... run on a Bio-Rad T100 Thermal Cycler (Hercules, CA, USA) with a PCR Master mix Solution i-MAX II (iNtRON Biotechnology, S Korea). PCR products were separated on a 1% agarose gel.
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No products found
because this supplier's products are not listed.
Sadis Matalon, et al.,
bioRxiv - Physiology 2023
Quote:
... The 8.1kb mtDNA was amplified by PCR and quantitatively analyzed by real-time PCR using specific primers provided in the Mouse Real-Time PCR Mitochondrial DNA Damage Analysis Kit from Detroit R&D (cat. #DD2M; Detroit, MI). Mitochondrial damage in the 8.1kb mtDNA fragment was quantified by a standard curve prepared ...
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No products found
because this supplier's products are not listed.
Mahanish J. Thapa, et al.,
bioRxiv - Genomics 2022
Quote:
... Gas-tight glass tubes with septa (2048-18150) and accessories (2048-11020 and 2048-10020) were purchased from Bellco Glass Inc ...
-
No products found
because this supplier's products are not listed.
Debatosh Das, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Real-time RT-PCR was performed with the qPCR GreenMaster high ROX mix (Jena Bioscience, Germany) and primers shown in Table S1 ...
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No products found
because this supplier's products are not listed.
Satoshi Imanishi, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... we performed a fluorescence based thermal shift assay using ProteoStat Thermal Shift Stability assay (ENZO Life Sciences, Farmingdale, NY, USA). 1 µg of KRAS G12D was mixed with DMSO (2.5 %) ...
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No products found
because this supplier's products are not listed.
Jennifer D. Noonan, Robin N. Beech,
bioRxiv - Molecular Biology 2022
Quote:
... Oocytes were injected with 50 nL (12.5 ng of each injected gene) of the desired cRNA subunit and accessory protein combination mix with the NanojectII (Drummond Scientific, USA). Following injection ...
-
No products found
because this supplier's products are not listed.
Jinze Li, et al.,
bioRxiv - Neuroscience 2023
Quote:
Blood flow in the mouse brain was recorded in real-time using an RFLSI III device (RWD Life Sciences, Shenzhen, China) throughout the whole process of ischemic stroke ...
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No products found
because this supplier's products are not listed.
Yanqing Huang, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Anirban Maitra that were authenticated by short tandem repeat profiling and were confirmed to be Mycoplasma free by real-time PCR (CellCheck Mouse 19 Plus, IDEXX Laboratories, Inc.). For the flank model ...
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No products found
because this supplier's products are not listed.
Ellen M. Rodberg, et al.,
bioRxiv - Neuroscience 2022
Quote:
... body temperature was maintained with deltaphase thermal pads (Braintree Scientific, Braintree, MA). Animals were placed into a stereotaxic frame (David Kopf Instruments ...
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No products found
because this supplier's products are not listed.
Samir Malhotra, et al.,
bioRxiv - Bioengineering 2022
Quote:
... were from Real Biotech Corporation (RBC ...
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No products found
because this supplier's products are not listed.
Kelsilandia A. Martins, et al.,
bioRxiv - Microbiology 2020
Quote:
... Mice were kept at a constant temperature on a thermal blanket (Fine Science Tools, Vancouver, Canada) during procedures ...
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No products found
because this supplier's products are not listed.
Jimin Yoon, et al.,
bioRxiv - Biophysics 2023
Quote:
The following cell lines were only used for cellular thermal shift assays: MDCK (American Type Culture Collection) and HEK 293A (Thermo Fisher ...
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No products found
because this supplier's products are not listed.
Numan Ibne Asad, et al.,
bioRxiv - Microbiology 2022
Quote:
... flour peak maximum time (PMT; time for the dough to reach its maximum consistency following hydration) ...
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No products found
because this supplier's products are not listed.
Junhwan Kwon, et al.,
bioRxiv - Neuroscience 2022
Quote:
The tapered glass fibers were obtained by the thermal drawing of a glass rod (1.5 mm in diameter) using a micropipette puller (P-1000, Sutter Instrument). To mimic the typical axon diameters (1–5 μm) ...
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No products found
because this supplier's products are not listed.
Kristina Bartmann, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... washed three times with PBS (PAN Biotech), and then incubated overnight at 4°C with primary antibodies diluted in blocking solution [5% Goat Serum (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Aditya Singh, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and b) relative partner-interaction time (RPI), defined as the ratio of time spent interacting with (pointed towards ...
-
No products found
because this supplier's products are not listed.
Sergio Gutiérrez, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... analysis of agarwood and distillate extracts in acetone was performed using an Agilent 7890B gas-chromatography system equipped with a Zoex ZX1 cryogenic thermal modulator and a JEOL TOF MS (AccuTOF GCx-plus, JEOL, Japan). The GCxGC system featured a normal (non-polar x mid-polar ...
-
No products found
because this supplier's products are not listed.
Qi Wang, et al.,
bioRxiv - Genomics 2023
Quote:
... and RNasin Plus (Promega)] 10-15 times using pestle A “loose” followed by pestle B “tight” 10-15 times (DWK Life Sciences, Millville, NJ, USA). Homogenate was passed through a 70 µm 1.5 ml mini strainer (PluriSelect ...
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No products found
because this supplier's products are not listed.
Ruobing Wang, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and Histoclear (Biocare Medical, 3 times 15 minutes each), and incubated in paraffin (3 changes 30 minutes each ...
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No products found
because this supplier's products are not listed.
Thomas HB FitzGerald, et al.,
bioRxiv - Neuroscience 2019
Quote:
... agents update beliefs about all states within a fixed-length time window that includes the present time but stretches a set distance into the past (Figure 1) (FitzGerald et al., 2017). This window moves forward in time at the same rate that observations are gathered ...
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No products found
because this supplier's products are not listed.
Arnaud Taton, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... at which time cryptomaldamide eluted as a single peak (Phenomenex Aqua Kinetex 5μ C18 125Å ...
-
No products found
because this supplier's products are not listed.
Lizhi He, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... lysed by grinding 50 times in Wheaton A (Wheaton, 357538), and then incubated on ice for 20 minutes ...
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No products found
because this supplier's products are not listed.
Nicole A. Jandick, Cathy L. Miller,
bioRxiv - Microbiology 2023
Quote:
... at which time Cell Counting Kit (CCK)-8 reagent (APExBIO, K1018) was added to the cells according to manufactures instructions ...
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No products found
because this supplier's products are not listed.
Thibault Courtellemont, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Cells were passed one time through a French press (Constant Systems LTD) at 4°C with 2.2 kpsi of pressure ...
-
No products found
because this supplier's products are not listed.
Shiro Jimi, et al.,
bioRxiv - Pathology 2021
Quote:
... rabbit anti-mouse CD31 antibody (30-time dilution: Dianova GmbH, Hamburg, Germany) and rabbit anti-mouse α-smooth muscle actin (SMA ...
-
No products found
because this supplier's products are not listed.
Chao Hu, et al.,
bioRxiv - Immunology 2020
Quote:
... the plate was washed 6 times and the pNPP solution (Mabtech, Sweden) was added at 50 μl per well ...
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No products found
because this supplier's products are not listed.
Christopher M. Bourne, et al.,
bioRxiv - Immunology 2023
Quote:
... at which time D-luciferin substrate (Gold Biotech, St. Louis, Missouri, USA) was added at a final concentration of 0.5 µg/µL to each well ...
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No products found
because this supplier's products are not listed.
Liheng Wang, et al.,
bioRxiv - Cell Biology 2020
Quote:
... for 20-25 times followed by low retention and regular orifice tips (Rainin) for 10 times ...
-
No products found
because this supplier's products are not listed.
Andrés R. Muñoz-Rojas, et al.,
bioRxiv - Immunology 2020
Quote:
... Cells were then stimulated at the indicated doses and times using LPS (Invivogen), IFN-γ (Peprotech ...
-
No products found
because this supplier's products are not listed.
Nico Wahl, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Reaction was washed two additional times before pAG-MNAse (15-1016-EPC Epicypher) was added 1:20 in washing buffer and incubated for 1h at 4°C ...
-
No products found
because this supplier's products are not listed.
Colin P Sharp, et al.,
bioRxiv - Microbiology 2022
Quote:
... washed six times with TBS-T and incubated with HRP-conjugated Streptavidin (Proteintech) in 5%BSA/TBS-T for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Noah J Harris, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Vesicles were extruded 11 times through a 150nm NanoSizer Liposome Extruder (T&T Scientific) and stored at −80°C.
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Sample-loading buffer and protein stain in one
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Yashwanth Ashok, et al.,
bioRxiv - Microbiology 2019
Quote:
... Plates were washed four times with TBST after which chemiluminescent substrate (Advansta, WesternBright Quantum) was added and subsequent chemiluminescence was detected using Hidex Sense microplate reader (Hidex).
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No products found
because this supplier's products are not listed.
Yoshiaki Kinosita, et al.,
bioRxiv - Microbiology 2020
Quote:
... archaellar filaments were sheared by 30 times pipetting with 200 μl pipette (F123601, Gilson), infused into a flow chamber and kept for 10 min ...
-
No products found
because this supplier's products are not listed.
Hilal Yeter-Alat, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Blots were washed two times at 42°C with 2X SSC buffer (Euromedex, Souffelweyersheim, France) with 0.1% SDS added ...
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No products found
because this supplier's products are not listed.
Akansha Mishra, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... and obtaining blood at time 0 and 15 min to measure serum insulin using ELISA (Alpco). ITT was performed after overnight fasting by 0.75 mU/g BW regular insulin i.p ...
-
No products found
because this supplier's products are not listed.
Marco Pitino, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The assay plate was washed four times with a wash solution provided by ELISA kit (RayBiotech). HRP-conjugated Anti-IgG was added to plate post wash and incubated at room temperature for one hour with gentle shaking ...
-
No products found
because this supplier's products are not listed.
Clara Lambert, et al.,
bioRxiv - Microbiology 2023
Quote:
... and identified by comparing to retention times of purified esterified FA standards (Mixture ME100, Larodan, Sweden). Results are shown as percent of the specific FA compared to total peak areas (TotalChrom Workstation ...
-
No products found
because this supplier's products are not listed.
Johanna Luige, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 3 times 30 seconds ON/OFF sonication was carried out at high intensity (Covaris S2 Focus Ultrasonicator) to disrupt the chromatin and release nuclear proteins ...
-
No products found
because this supplier's products are not listed.
Jennifer N. Chousal, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... The embryos were then washed three times with PBST and incubated with DAPI (Novus Biologicals, NBP2-31156) at a dilution of 1:500 for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Karolina Honzejkova, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 3.5 μL of protein solution was applied to a freshly glow-discharged (45 s total time, Gatan Solarus II 955 (Gatan ...
-
No products found
because this supplier's products are not listed.
Peter Farkas, Teresa B. Fitzpatrick,
bioRxiv - Plant Biology 2024
Quote:
... The membrane was washed in TBST four times and incubated with goat anti-rabbit secondary antibody (Agrisera) conjugated to horseradish peroxidase in a 1:3000 dilution in blocking solution for 1 hour at room temperature (ca ...
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No products found
because this supplier's products are not listed.
Michelle S Glossop, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Coverslips were then washed 3 times in cold PBS and fixed in cold 4% paraformaldehyde (Alfa Aesar) in PBS for 15 min ...
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No products found
because this supplier's products are not listed.
Ranjie Xu, et al.,
bioRxiv - Neuroscience 2019
Quote:
... PSD95 antibody (2.5 μg/ml) was incubated with five times PSD95 blocking peptide (12.5 μg/ml, Synaptic System) or equivalent amount of PBS at RT for 30 minutes before applying secondary antibodies to the brain tissues as suggested by the manufacturer instructions.
-
No products found
because this supplier's products are not listed.
L Llaó Cid, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... incubation times and appropriate antibody concentrations were optimized using the Opal™ 5-color kit (Akoya Biosciences, NEL840001KT). Epitope stability was assessed to determine the order of each antibody in sequence ...
-
No products found
because this supplier's products are not listed.
Margot Brandt, et al.,
bioRxiv - Genomics 2020
Quote:
... RNA was extracted at the designated time point by adding 500 uL of IBI Isolate DNA/RNA Reagent (IBI Scientific) directly to cells on the plate and stored at −80C until extraction and RNA-sequencing ...
-
No products found
because this supplier's products are not listed.
Jan Schlegel, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... After bilayer formation beads were washed two more times with PBS and 5pmol of His-tagged proteins added (Sino Biological: ACE2-His 10108-H08H ...
-
No products found
because this supplier's products are not listed.
Katarzyna M. Luda, et al.,
bioRxiv - Immunology 2022
Quote:
... and re-cultured (2.5 × 106 cells/well in 24-well plates) for indicated times in medium containing 13C-labeled metabolites (Cambridge Isotope Laboratories) at the following concentrations ...
-
No products found
because this supplier's products are not listed.
Xiaoyu Zhao, et al.,
bioRxiv - Immunology 2023
Quote:
... The ELISA plates were washed three times with PBST and then 100 μl of 5,000-fold diluted anti-Strep-Tactin HRP conjugate antibody (IBA Life Science) was added into each well for another 1 h at 37 °C ...