-
No products found
because this supplier's products are not listed.
Tracy M. Smith, et al.,
bioRxiv - Microbiology 2022
Quote:
... Real-time quantitative PCR was performed in EU Fast Cycler PCR 96-well plates (BIOplastics) using the StepOnePlus Real-Time PCR System (v ...
-
No products found
because this supplier's products are not listed.
Madeleine A. G. Gilbert, et al.,
bioRxiv - Pathology 2023
Quote:
... ZEISS Cryo-accessories tool kit and Omniprobe 350 cryo-micromanipulator (Oxford Instruments) and operated at 30 keV ...
-
No products found
because this supplier's products are not listed.
Tongtong Ma, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Real-time quantitative PCR was performed using SYBR Green Mix (Abclonal, #RK21203) following manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Luke E. Holman, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... a Primer Design Real-Time PCR Internal Control Kit (PrimerDesign, Southampton, United Kingdom) was used ...
-
No products found
because this supplier's products are not listed.
Helen M. Wise, et al.,
bioRxiv - Microbiology 2019
Quote:
... the accessory lobe was cut into small pieces and homogenised with a gentleMACS Octo Dissociator (Miltenyi Biotec) using C tubes (Miltenyi Biotec ...
-
No products found
because this supplier's products are not listed.
Scott H. Saunders, et al.,
bioRxiv - Microbiology 2019
Quote:
... and then annealed on a thermocycler (Beckman Instruments) by initial heating to 90 °C followed by slow cooling over a span of 90 min.
-
No products found
Youkun Bi, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Real-time PCR was performed using the SYBR Green PCR kit(Applied Biological Materials, ABM) and a Rotor-Gene-Q instrument (QIAGEN) ...
-
No products found
because this supplier's products are not listed.
Chunhui Zhai, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The real-time images of sEV were recorded by a sCMOS camera (Prime TM; Photometrics). A motorized XY stage (Ludl Electronic Products ...
-
No products found
because this supplier's products are not listed.
Fuqiang Cui, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Primers were designed with Real-time PCR (TaqMan) Primer and Probes Design Tool from GenScript wesite (www.genscript.com/tools/real-time-pcr-taqman-primer-design-tool ...
-
No products found
because this supplier's products are not listed.
Jackson M. Baumann, et al.,
bioRxiv - Physiology 2024
Quote:
... SYBR Green based real-time PCR was performed using Apex qPCR Master Mix (Genesee Scientific). PCR conditions were as follows ...
-
No products found
because this supplier's products are not listed.
Ya.V. Solovev, et al.,
bioRxiv - Plant Biology 2020
Quote:
... thermocycler using the ScreenMix-HS kit (Evrogen, Moscow, Russia). The PCR cycling conditions were as follows ...
-
No products found
because this supplier's products are not listed.
Xiaoxuan Zhao, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The resulting cDNA was subjected to quantitative real-time PCR using the SYBR Green kit (Solarbio) on an Exicycler TM 96 instrument (Bioneer ...
-
No products found
because this supplier's products are not listed.
Ryota Maeda, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Real-time binding experiments were performed using an Octet Red96 instrument (fortèBIO, Pall Life Science, Portsmouth, NH). Each purified nanobody clone was biotinylated with EZ-Link Sulfo-NHS-LC-Biotin (Thermo ...
-
No products found
because this supplier's products are not listed.
Anderson R Frank, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... mice with jugular vein catheters (surgical code: JUGVEIN) and transcutaneous one-channel vascular access buttons (accessory code: INSTBUTON1CH) were purchased from Charles River Laboratories and individually housed at UT Southwestern Medical Center ...
-
No products found
because this supplier's products are not listed.
Emilie Doz-Deblauwe, et al.,
bioRxiv - Immunology 2023
Quote:
... total RNA was extracted from FACS-sorted neutrophils from the right accessory lung lobe homogenized using Lysing matrix D tubes from MP Biomedicals and a Precellys® using a NucleoSpin RNA kit with DNase treatment (Macherey Nagel) ...
-
No products found
because this supplier's products are not listed.
Sushama Telwatte, et al.,
bioRxiv - Microbiology 2021
Quote:
... The log-linear relationship between viral load measured by RT-PCR (Abbott Real Time SARS-CoV-2 assay) and RT-ddPCR was determined using GraphPad Prism (version 8.4.1).
-
No products found
because this supplier's products are not listed.
Lisa Goers, et al.,
bioRxiv - Microbiology 2022
Quote:
... or 4×104 cells/well (for real-time cytotoxicity assay) into 96-well tissue culture treated plates (Corning 3603 for plate reader, Greiner μClear #655096 for automated microscopy assays ...
-
No products found
because this supplier's products are not listed.
Rui Sun, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Quantitative real-time PCR reactions with Taq polymerase prepared following (Pluthero, 1993) and SYBR Green I Nucleic Acid Gel Stain (Lonza) was performed in triplicates using the CFX96 real-time PCR detection system (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Kristen E. Rohli, et al.,
bioRxiv - Cell Biology 2021
Quote:
... were either generated by heterozygous cross and genotypes confirmed via real time PCR according to Jackson Laboratories or directly purchased from Jackson Laboratories. Hyperglycemic db/db mice (ad lib fed blood glucose > 220 mg/dl ...
-
No products found
because this supplier's products are not listed.
Yapeng Su, et al.,
bioRxiv - Bioengineering 2019
Quote:
... MART1and PFK transcripts were analyzed by SYBR Green–based real-time quantitative RT-PCR (qRT-PCR) using specific primers purchased from Santa Cruz. Data were normalized to the expression of RPL19 and are expressed as fold changes.
-
No products found
because this supplier's products are not listed.
Katarzyna Zwolinska, et al.,
bioRxiv - Microbiology 2022
Quote:
... Viral genome equivalents (vge) were determined by real-time quantitative PCR (qPCR) of encapsidated DNA isolated using the NucleoSpin Blood QuickPure (Macherey-Nagel; 740569.250). To generate mutated quasiviruses ...
-
No products found
because this supplier's products are not listed.
Davide Monteferrario, et al.,
bioRxiv - Bioengineering 2024
Quote:
Chromatin immunoprecipitation followed by real-time quantitative PCR (ChIP-qPCR) was performed using the ChIP-IT PBMC kit (53042; Active Motif, Carlsbad CA) according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Guillaume Le Goc, et al.,
bioRxiv - Animal Behavior and Cognition 2021
Quote:
... It is stuck on two 78W Peltier modules (Adaptive) with thermal tape (3M). A transparent ...
-
No products found
because this supplier's products are not listed.
Marziyeh Belal, et al.,
bioRxiv - Physiology 2022
Quote:
... then washed three times (each time 10 min) and subsequently mounted with Fluorsave medium (Calbiochem). The controls were the sections where primary antibody was omitted ...
-
No products found
because this supplier's products are not listed.
Emily M. Carvalho, et al.,
bioRxiv - Bioengineering 2024
Quote:
... nominal spring constant 0.08 N/m) were calibrated using the thermal method and one 25-μm diameter polystyrene sphere (07313-5, Polysciences) was affixed to the end of each cantilever with a small amount of UV-cured glue (AA 349 ...
-
No products found
because this supplier's products are not listed.
Arunima Bhattacharjee, et al.,
bioRxiv - Microbiology 2021
Quote:
... the sections were coated with ∼15 nm carbon using a thermal evaporation method (108C Auto Carbon Coater, Ted Pella, Inc.) and were secured with Cu sticky tape on the SEM stage ...
-
No products found
because this supplier's products are not listed.
Heidi A. Arjes, et al.,
bioRxiv - Microbiology 2021
Quote:
... The plate stacker and the front of the microplate reader were enclosed in a custom- fabricated box along with a thermal control unit (AirTherm SMT, World Precision Instruments) to ensure a constant temperature of 37 °C during growth curve measurements ...
-
No products found
because this supplier's products are not listed.
Matthew G. Blango, et al.,
bioRxiv - Microbiology 2021
Quote:
... The retention time of i6a from biological extracts was consistent with the retention time of authentic i6a material (Cayman Chemical). Absolute intensities of the i6a ions were retrieved from the mass spectrum scanned between 15.4 – 16.1 minutes ...
-
No products found
because this supplier's products are not listed.
Denise Pajonczyk, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
FPR-mediated inhibition of cellular de novo cAMP formation was quantified by competitive immunoassays based on the time-resolved measurement of fluorescence resonance energy transfer (HTRF, Homogeneous Time-Resolved Fluorescence) using the cAMP-Gi Kit from Cisbio as previously published [37] ...
-
No products found
because this supplier's products are not listed.
Xinke Zhang, et al.,
bioRxiv - Bioengineering 2021
Quote:
... time mated Sprague–Dawley rats (Envigo, Livermore, CA) were dosed with 40 mg/kg of all-trans retinoic acid (RA ...
-
No products found
because this supplier's products are not listed.
Catherine A. Bellissimo, et al.,
bioRxiv - Physiology 2023
Quote:
... membranes were washed three times (5 minutes each time) in TBS-T and incubated at room temperature with appropriate fluorescent secondary antibody (LI-COR). Prior to detection ...
-
No products found
because this supplier's products are not listed.
Lun Zhang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The resulting liposomes were extruded five times through a 200 nm polycarbonate membrane and five times through a 100 nm membrane (Avanti Polar Lipids) using an Avanti Mini Extruder (Avanti Polar Lipids) ...
-
No products found
because this supplier's products are not listed.
Tiziano A. Schweizer, et al.,
bioRxiv - Immunology 2021
Quote:
... or 8-well microslides (ibidi, for microscopy time lapse). Plates were incubated for 4 h-18 h at 37°C + 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Shruthi Sriramkumar, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... After the incubation time ALDEFLUOR assay (Stem Cell Technologies, #01700) was performed as described previously (14).
-
No products found
because this supplier's products are not listed.
Jingyu Li, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... coupled to a quadrupole time-of-flight system (AB Sciex TripleTOF 6600 ...
-
No products found
because this supplier's products are not listed.
Anna Zawistowska-Deniziak, et al.,
bioRxiv - Immunology 2022
Quote:
... The membranes were washed three times with 1× Wash Buffer (R&D Systems) to remove unbound proteins ...
-
No products found
because this supplier's products are not listed.
Kai He, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Real-time PCR was performed using BlazeTaq SYBR Green qPCR Mix 2.0 (GeneCopoeia, QP031) with CFX384 Real-Time system (BIO-RAD).
-
No products found
because this supplier's products are not listed.
Susan Paton, et al.,
bioRxiv - Microbiology 2021
Quote:
... RT-PCR was performed using the VIASURE SARS-CoV-2 Real Time PCR Detection Kit (Viasure; CerTest Biotec, Zaragoza, Spain), following the methods provided ...
-
No products found
because this supplier's products are not listed.
NC Rodgers, et al.,
bioRxiv - Cell Biology 2022
Quote:
... microtubules were diluted 56 times into 10 μM Taxol (Tocris) in MRB80 (MRB80T ...
-
No products found
because this supplier's products are not listed.
Weiran Feng, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... washed three times in HistoClear II (National Diagnostics, HS-202), and mounted in Permount (Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Uli Schmitz, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... Plates were washed three times with 0.1% Tween-20 (Amresco; 0777)/1 X DPBS % v/v solution (DPBST ...
-
No products found
because this supplier's products are not listed.
Dorota D. Klysz, et al.,
bioRxiv - Immunology 2023
Quote:
1×106 cells were washed with two times with PBS (Rockland) and then resuspended in 1ml of 250nM cisplatin and PBS (Fluidigm ...
-
No products found
because this supplier's products are not listed.
Susheel K. Gunasekar, et al.,
bioRxiv - Physiology 2021
Quote:
... 15 and 30 min time points in microvette capillary tubes (SARSTEDT, #16.444) and centrifuged at 2000Xg for 20 min at 4°C ...
-
No products found
because this supplier's products are not listed.
Nils Burger, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Membranes were washed three times with TBS + 0.1% Tween (Boston BioProducts, USA) and membranes were scanned using an Odyssey DLx (LI-COR Biosciences ...
-
No products found
because this supplier's products are not listed.
Alexander T. Dils, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were incubated for indicated times with 10ng/mL of murine IL-7 (PeproTech) in complete media.
-
No products found
because this supplier's products are not listed.
Erdem D. Tabdanov, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... conditions and imaged immediately after designated incubation time as per commercial protocol (Cytoskeleton Inc.). Briefly ...
-
No products found
because this supplier's products are not listed.
Brajabandhu Pradhan, et al.,
bioRxiv - Microbiology 2020
Quote:
... and bead beating 4 times for 2 minutes in a Mini-BeadBeater-8 (BioSpec) with cooling on ice in between.
-
No products found
because this supplier's products are not listed.
Pan Chen, Sampada Mishra, Daniel L. Levy,
bioRxiv - Cell Biology 2023
Quote:
... three times to facilitate egg adhesion on protamine-coated glass-bottom dishes (MatTek Corporation). Unfertilized eggs were transferred to protamine-coated glass-bottom dishes for a maximum time of 15 minutes before microinjection and fertilization ...
-
No products found
because this supplier's products are not listed.
Nicole M Sodir, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... and four times with 200ug rat anti-mouse CD8 (Clone 2.43, Bio X Cell, BE0061) every other day ...
-
No products found
Karla G. Espinosa, et al.,
bioRxiv - Genetics 2021
Quote:
... embryos at each respective time point were digested with collagenase type II (LS004176, Worthington Biochemical Corporation) and plated on 12 mm circular coverslips ...