-
No products found
because this supplier's products are not listed.
Jonathan Chau, et al.,
bioRxiv - Genetics 2020
Quote:
Lentiviruses carrying shRNA plasmids (MISSION shRNA, Sigma-Aldrich) for each DUX4 target gene ...
-
No products found
because this supplier's products are not listed.
Vasiliki Karalis, et al.,
bioRxiv - Neuroscience 2021
Quote:
The AAV-Tet3-shRNA plasmid (Addgene plasmid # 85740) was used as a backbone to generate the AAV9-hU6-shRptor-EYFP construct ...
-
No products found
because this supplier's products are not listed.
Xiaohuan Chen, et al.,
bioRxiv - Cell Biology 2024
Quote:
... The scrambled shRNA and IP3R1 shRNA plasmids were purchased from OriGene; the 29mer sequence of scrambled shRNA (Cat# TR30015 ...
-
No products found
because this supplier's products are not listed.
Shiyi Wang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... cultured rat astrocytes were transfected with shRNA and/or expression plasmids using Lipofectamine LTX with Plus Reagent (Thermo Scientific) per the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Hephzi Tagoe, et al.,
bioRxiv - Cell Biology 2022
Quote:
Alox12b and Tgm1 shRNA knockdown in rat epidermal keratinocytes using SureSilencing shRNA plasmids (Qiagen) has been previously described (O’Shaughnessy et al ...
-
No products found
because this supplier's products are not listed.
Anupriya Aggarwal, et al.,
bioRxiv - Microbiology 2021
Quote:
... shRNA plasmids (pool of three shRNA plasmids per target) were obtained directly from Santa Cruz.
-
No products found
because this supplier's products are not listed.
Amr Abouelezz, et al.,
bioRxiv - Neuroscience 2019
Quote:
... shRNA against rat Tpm3.1 (NM_173111.1) was purchased from GeneCopoeia (RSH053175-33-mH1 ...
-
No products found
because this supplier's products are not listed.
Rohtem Aviram, et al.,
bioRxiv - Cell Biology 2024
Quote:
... MISSION® shRNA lentiviral plasmids (Merck) were used for knockdown of LOX (shLOX1 – TRCN0000045991 ...
-
No products found
because this supplier's products are not listed.
Lisa Baker, et al.,
bioRxiv - Neuroscience 2020
Quote:
... except cells were transduced with AAV5 containing a control scrambled shRNA plasmid or a FL2 shRNA plasmid (Vector Biolabs). shRNA sequences were cloned into a D2-U6 vector ...
-
No products found
because this supplier's products are not listed.
Adam G Grieve, et al.,
bioRxiv - Biochemistry 2020
Quote:
... in 35mm plates with 0.5 μg of pLKO shRNA or pLVX expression plasmids (Takara), 0.35 μg pCMV-dR8.2 and 0.15 μg pCMV-VSVG ...
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No products found
because this supplier's products are not listed.
Natalia G. Sampaio, et al.,
bioRxiv - Immunology 2021
Quote:
... and 1 μg shRNA plasmid using Fugene 6 (Promega). The next day ...
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No products found
Md Imamul Islam, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Scramble (LV-shRNA-scramble, #LVP015-G) and Trx1 shRNA plasmids (LV-shRNA-Trx1, #488660940395) were purchased from Applied Biological Materials Inc. ...
-
No products found
because this supplier's products are not listed.
Ulli Heydasch, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Transfection of plasmids for knockdown purposes (shRNA-vectors) was performed using FuGene®HD (Roche Diagnostics) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Kush K. Yadav, et al.,
bioRxiv - Microbiology 2023
Quote:
... plasmid DNA encoding rat HEV was linearized using EcoRI (NEB). Five percent of the reaction was subjected to gel electrophoresis with ethidium bromide staining and visualized with ultraviolet light to verify that linearization had occurred.
-
No products found
because this supplier's products are not listed.
David V.C. Brito, et al.,
bioRxiv - Neuroscience 2020
Quote:
... we used adult male C57BL/6N mice that were 8 weeks old at the time of surgery [(MeCP2-shRNA (n=8) or Control-shRNA (n=8)] (Charles River, Sulzfeld, Germany). The mice were group-housed on a 12h light/dark cycle with ad libitum access to food and water ...
-
No products found
because this supplier's products are not listed.
Ratnadeep Mukherjee, et al.,
bioRxiv - Immunology 2020
Quote:
... 5 µg of NOSIP shRNA plasmids or control TRC2 plasmid was mixed with 100 µl Cell Line Nuceleofector Solution V (Lonza) and added to one million cells ...
-
No products found
because this supplier's products are not listed.
Sanghee Yun, et al.,
bioRxiv - Animal Behavior and Cognition 2023
Quote:
... Each annealed oligonucleotide was ligated into the adeno-associated virus (AAV2) plasmid (pAAV-EGFP-shRNA; Stratagene, La Jolla, CA)(Hommel et al. ...
-
No products found
because this supplier's products are not listed.
Abir Mukherjee, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... 5 million stable SKOV3ip1 cells (transduced with either control shRNA or shRNA targeting HIF1α) were injected into female athymic nude mice (Envigo), and tumors were allowed to establish for 4 weeks ...
-
No products found
because this supplier's products are not listed.
Cornelius M. Funk, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 1,500–4,000 cells containing either a Dox-inducible non-targeting control shRNA or EIF4EBP1-targeting specific shRNAs were seeded in Costar Ultra-low attachment plates (Corning) for 8–14 d in 150 µl growth medium ...
-
No products found
because this supplier's products are not listed.
Hannah L. Mackay, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... Rat αBrdU (Abcam) used at a concentration of 1:1000 and Mouse αBrdU (BD Biosciences ...
-
No products found
because this supplier's products are not listed.
Nerea Berastegui, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... two different short hairpin RNAs (shRNAs) and a scramble shRNA were cloned into pSIH1-H1-copGFP shRNA lentiviral vector (System Biosciences #SI501A-A). Generation of lentiviruses and further specifications are described in supplemental methods.
-
No products found
because this supplier's products are not listed.
Danira Toral-Rios, et al.,
bioRxiv - Neuroscience 2023
Quote:
... anti-rat CD68 (rat monoclonal, 1:400; Biorad, MCA1957), was incubated for 2 h at 37°C and removed by rinsing the cells 3 times with TBS ...
-
No products found
because this supplier's products are not listed.
Hannah A. Pizzato, et al.,
bioRxiv - Immunology 2023
Quote:
... rat IgG2b (BioXCell), CD8 (clone YTS169.4 ...
-
No products found
because this supplier's products are not listed.
Ola Bagato, et al.,
bioRxiv - Immunology 2023
Quote:
... rat (Biolegend, # 400602) and mouse (Biolegend ...
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No products found
because this supplier's products are not listed.
Farid F. Kadyrov, et al.,
bioRxiv - Immunology 2023
Quote:
... Sections were then stained with primary antibody diluted in 1% BSA in PBS for 1 hour (rat anti CD34 abcam ab8158 1:200 dilution, rat anti CD68 BioLegend 137002 1:400 dilution, rat anti Ly6g BD Pharmingen 551459 1:100 dilution ...
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No products found
because this supplier's products are not listed.
Caroline J. Zeiss, et al.,
bioRxiv - Microbiology 2021
Quote:
... Bound rat antibodies were detected with FITC-conjugated goat anti-rat IgG (Jackson ImmunoResearch).
-
No products found
because this supplier's products are not listed.
Valerio Taverniti, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... 38HK were transiently transfected with lentiviral shRNA plasmids using TransIT-Keratinocytes Transfection Reagent (Mirus) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Donald C. Koroma, et al.,
bioRxiv - Cell Biology 2021
Quote:
... were transfected with individual shRNA in pLKO.1 (Broad Institute, Cambridge, MA) mixed with packaging plasmid pCMV-dR8.91 (G193486, GenScript, Piscataway, NJ) and envelope plasmid pVSV-G (G178153 ...
-
No products found
because this supplier's products are not listed.
Hugo Girão, et al.,
bioRxiv - Cell Biology 2019
Quote:
... rat anti-RFP (ChromoTek) 1:1000 ...
-
No products found
because this supplier's products are not listed.
Xizi Chen, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Lentivirus expressing short-hairpin RNAs was prepared by transfecting PLKO.1 shRNA plasmids and packaging plasmids containing psPAX2 and pMD2.G into 293T cells using PEI (Polysciences). Collected conditional media containing virus particles were used to transduce cells in the growth media supplemented with Polybrene for 24 hours ...
-
No products found
because this supplier's products are not listed.
M. Inês Pascoal Ramos, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... mouse or rat collagen I (human, R&D Systems; mouse, Ray Biotech; rat, Yo Protein) or collagen III (human ...
-
No products found
because this supplier's products are not listed.
Uddhav Timilsina, et al.,
bioRxiv - Microbiology 2020
Quote:
... -anti-rat (Cell Signaling Technology), -anti-mouse (EMD Millipore ...
-
No products found
because this supplier's products are not listed.
Francesca Puca, et al.,
bioRxiv - Cancer Biology 2020
Quote:
The inducible shRNA was obtained from Cellecta by cloning into a pRSIT16-U6Tet-sh-CMV-TetRep-2A-TagRFP-2A-Puro vector the following hairpin sequence:
-
No products found
because this supplier's products are not listed.
Gregory A. Viglianti, et al.,
bioRxiv - Microbiology 2020
Quote:
... or a mixture of three to five control shRNAs (Invivogen) and a blasticidin-resistance gene using Oligofectamine (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Shivangi Khanna, et al.,
bioRxiv - Cell Biology 2022
Quote:
All shRNA’s (GIPZ Lentiviral constructs) were purchased from Dharmacon (GE Healthcare). Pol κ shRNA (RMM4532-EG27015 ...
-
No products found
because this supplier's products are not listed.
Gonzalo Quiroga-Artigas, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Newly synthesized shRNAs were then purified using the Direct-zol™ RNA MiniPrep Kit (Zymo Research), eluted in 35μl of nuclease-free H2O and quantified with a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Tianxiong Yu, et al.,
bioRxiv - Genetics 2019
Quote:
... and rat (Illumina). RNAs longer than 200 nt were selectively recovered using the RNA Clean & Concentrator-5 kit (Zymo Research) ...
-
No products found
because this supplier's products are not listed.
Joachim M. Surm, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
... shRNAs were synthesized with AmpliScribe T7-Flash Transcription Kit (Epicentre, Charlotte, NC) for 15 h followed by 15 min treatment with 1 µl of DNase I ...
-
No products found
because this supplier's products are not listed.
Kristina Vaeth, et al.,
bioRxiv - Biochemistry 2021
Quote:
... anti-rat-HRP (Dianova). All strains and plasmids used in this study are listed in Suppl ...
-
No products found
because this supplier's products are not listed.
Kazuhito Honjo, et al.,
bioRxiv - Immunology 2019
Quote:
... Fisher rats (Jackson Laboratory) were immunized at 3 to 4 day intervals over a 3 week period and popliteal nodes were fused with the mouse plasmacytoma Ag8.653 and plated for selection in 96-well plates (Won et al. ...
-
No products found
because this supplier's products are not listed.
Xinming Wang, et al.,
bioRxiv - Bioengineering 2021
Quote:
... mouse and rat (Miltenyi Biotec). Cardiomyocytes and non-cardiomyocytes were separated by Percoll gradient centrifugation ...
-
No products found
because this supplier's products are not listed.
Stephanie Veerasammy, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and rat anti-Ki67 (Vector Laboratories, Burlingame ...
-
No products found
because this supplier's products are not listed.
Niklas Simon, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... rat-anti-Ollas (L2, Novus Biologicals, NBP1-06713 ...
-
No products found
because this supplier's products are not listed.
Chen Liu, et al.,
bioRxiv - Cell Biology 2022
Quote:
... anti-rat (IRDye® 800 CW Goat anti-Rat IgG [H + L], LI-COR, 925-32219, 1:10.000) and anti-rabbit (IRDye ® 800 CW Goat anti-Rabbit IgG ...
-
No products found
because this supplier's products are not listed.
D. Czernecki, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... plasmids were isolated with NucleoSpin Plasmid QuickPure kit (Macherey-Nagel) and suspended in water ...
-
No products found
because this supplier's products are not listed.
Allen Sam Titus, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The rat DDR2/CD167b Gene ORF cDNA clone expression plasmid was obtained from Sino Biologicals (Beijing, China). Pcax Itgb1-FLAG was a gift from Dennis Selkoe and Tracy Young-Pearse (Addgene plasmid 30153 ...
-
No products found
because this supplier's products are not listed.
Surya Prakash Rao Batta, et al.,
bioRxiv - Cell Biology 2022
Quote:
... HUVECs expressing the shRNAs were plated in μ-Slide VI 0.4 (80606; IBIDI) at a density of 30,000cells per channel in starving medium (EBM+0.5%serum+doxy+puro) ...
-
No products found
because this supplier's products are not listed.
Jhon R. Enterina, et al.,
bioRxiv - Immunology 2021
Quote:
... anti-rat IgG2a-AF488 (SouthernBiotech), and anti-rat IgG2b-AF647 (BioLegend) ...
-
No products found
because this supplier's products are not listed.
Ya-Lin Lu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... E18 rat cortex (BrainBits®, FSDECX1M) were grown in STEMdiff™Neural Induction Medium (STEMCELL Technologies ...
-
No products found
because this supplier's products are not listed.
S. M. Nayeemul Bari, et al.,
bioRxiv - Microbiology 2021
Quote:
... Confirmed plasmids were purified using EZNA Plasmid Mini Kit (Omega Bio-tek), and where indicated ...