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magnetofection
difficult to transfect cells
Cat# KC30296,
PolyMag 100µL+PolyMag Neo100µL+ CombiMag 100µL+Magnetic Plate MF96000, USD $640.63/KIT
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Andrew S. Flies, et al.,
bioRxiv - Immunology 2020
Quote:
... Digested proteins in PBS were diluted 1:1 in Squalvax (Oz Biosciences # SQ0010) to a final concentration of 0.1 μg/μL and was mixed using interlocked syringes to form an emulsion ...
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No products found
because this supplier's products are not listed.
Haixia Zhang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Fresh rat serum (RS) was purchased from Lampire Biological Laboratories (Pipersville ...
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No products found
because this supplier's products are not listed.
Shogo Soma, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Rats received 100 nL of Fluoro-Gold (2.5% in H2O, Fluorochrome) and 1,200 nL of mRFP expressing G-deleted rabies viral vector (rHEP5.0-ΔG-mRFP ...
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No products found
because this supplier's products are not listed.
Mengqi Luo, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Membranes with protein samples subsequently reacted with anti-ACTB (1:5000, pAb, ZEN BIO, China), anti-SCRN2 (1:500 ...
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No products found
because this supplier's products are not listed.
Weigao Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 300 μL pure protein solution was added to a 1 mm pathlength quartz cuvette (Starna Cells, USA). The scanning wavelength starts from 300 nm and ends at 190 nm ...
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No products found
because this supplier's products are not listed.
Dakota R. Robarts, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... Extracted 8 point calibration curves were made using blank rat serum (Pel-Freez Biologicals) spiked with PFAS appropriate for dosed (100 – 2,500 ng/ml ...
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No products found
because this supplier's products are not listed.
Magdalena Malm, et al.,
bioRxiv - Systems Biology 2021
Quote:
... The expression of THBS4 in each sample was evaluated by sandwich ELISA using a human anti-HPC4-antibody (Icosagen) as capture antibody ...
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No products found
because this supplier's products are not listed.
Susanne Hellmuth, Olaf Stemmann,
bioRxiv - Cell Biology 2024
Quote:
... raised against CAKSKAKPPKGAHVEV = Cys + amino acids 1183-1197 of the human protein) and human anti-CREST (1:1,000; hct-0100, ImmunoVision). Secondary antibodies (all 1:500) ...
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No products found
because this supplier's products are not listed.
Fabio Tommasini, et al.,
bioRxiv - Bioengineering 2023
Quote:
Tissue slides were stained using the Movat Pentachrome Stain kit (Cat. # MPS-1, Scytek, USA). Briefly ...
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No products found
because this supplier's products are not listed.
Mingyu Fang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... gels were either stained with Coomassie (Protein Ark) or transferred to PVDF membranes for Western blot analysis.
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No products found
because this supplier's products are not listed.
Reza Nouri, et al.,
bioRxiv - Bioengineering 2022
Quote:
... LwaCas13a proteins were purchased from MCLAB (cat# CAS13a-100). Cas13a and crRNA were mixed in 1×PBS to form the non-activated Cas13a/crRNA at room temperature for 20 min and stored at -80°C ...
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No products found
because this supplier's products are not listed.
Benjamin Abrams, et al.,
bioRxiv - Cell Biology 2022
Quote:
All study participants received a sample kit which included: 1) 500 µL of CyGELTM mounting media (BioStatus Ltd., Cat# Cy10500), 2 ...
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No products found
because this supplier's products are not listed.
Gabriela O. Bodea, et al.,
bioRxiv - Genomics 2023
Quote:
... MeCP2: We quantified MeCP2 protein expression using Imaris 9.5.1 (Bitplane, Oxford Instruments and we analyzed two hippocampal sections per animal ...
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No products found
because this supplier's products are not listed.
K Pilarzyk, et al.,
bioRxiv - Neuroscience 2022
Quote:
... PDE11A (FabGennix PD11-112 at 1:100; Aves custom PDE11A4 #1 at 1:10,000; Fabgennix PPD11A-140AP at 1:1000; Fabgennix PPD11A-150AP at 1:500), NF-L (Cell Signaling #2837 at 1:10,000) ...
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No products found
because this supplier's products are not listed.
Nils H. Rustmeier, et al.,
bioRxiv - Biochemistry 2023
Quote:
... proteins were incubated with oligosaccharides (Forssman antigen trisaccharide: Elicityl, France; Blood group A trisaccharide: Biosynth, UK) for 5 minutes prior to their addition to the cellular samples (∼1×106 cells in 100 µL) ...
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No products found
because this supplier's products are not listed.
Venkatesh Mallikarjun, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Proteins from cell pellets were solubilised by bead-beating using six 1.6 mm steel beads (Next Advance) at 4 °C in 30 µL 1.1% (w/v ...
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No products found
because this supplier's products are not listed.
Wensheng Chen, Darrell Pilling, Richard H. Gomer,
bioRxiv - Immunology 2022
Quote:
... or 1 or 10 μM IN-1 (AdooQ, Irvine, CA) from a 10 mM stock in DMSO (VWR ...
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No products found
because this supplier's products are not listed.
Kritika S. Katiyar, et al.,
bioRxiv - Bioengineering 2019
Quote:
... 1% FBS (Atlanta Biologicals), 2.5 mg/mL glucose (Sigma) ...
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No products found
because this supplier's products are not listed.
Ewan Phillip Ramsay, et al.,
bioRxiv - Biochemistry 2020
Quote:
HeLa cells were transfected with a 1:1:1 mix of gRNA1 and gRNA2 vectors together with the donor plasmid using PolyJet transfection reagent (SL100688, SignaGen Laboratories) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Liqiang Zhang, et al.,
bioRxiv - Microbiology 2022
Quote:
... Serp-1 is incubated with mPEG-NHS (5 K) (Nanocs Inc., #PG1-SC-5k-1, NY) in PBS buffer (pH 7.8 ...
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No products found
because this supplier's products are not listed.
Moitrayee Bhattacharyya, et al.,
bioRxiv - Biophysics 2019
Quote:
... the glass substrates were treated with a mixture of Poly-L-lysine PEG and PEG-Biotin (1000:1, both at 1 mg/mL) for 30 minutes (SuSoS, Dübendorf, Switzerland). The glass substrates were then washed with 2 mL of Dulbecco’s phosphate-buffered saline (DPBS ...
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No products found
because this supplier's products are not listed.
Jin-Ran Chen, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Serum bone resorption marker C-terminal telopeptides of type I collagen (CTX-1) was measured by Rat-LapsTM ELISA from Nordic Biosciences Diagnostic (Herlev ...
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No products found
because this supplier's products are not listed.
Eun Jeong Lee, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Plasma ACTH concentrations were measured using the ACTH ELISA kit (MD Biosciences), according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Amanda P. Waller, et al.,
bioRxiv - Pathology 2020
Quote:
... ELISA and immunoblot antibodies were validated using species-specific positive (purified species-specific protein; Haematologic Technologies, Inc, Essex Juntion, VT) and non-specific protein negative controls ...
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No products found
because this supplier's products are not listed.
Kathleen M.E. Gallagher, et al.,
bioRxiv - Immunology 2021
Quote:
A qualitative ELISA for Human Anti-IgG/A/M SARS-CoV-2 ELISA (The Binding Site) was performed using donor serum per manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Renata Varnaitė, et al.,
bioRxiv - Immunology 2020
Quote:
... SARS-CoV-2 specific IgM antibodies were detected using EDI Novel Coronavirus COVID-19 IgM ELISA kit (Epitope Diagnostics), according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Sung-Eun Choi, et al.,
bioRxiv - Pathology 2020
Quote:
... Total protein in the hydrolysed sample was also measured using the Total Protein Assay Kit (QuickZyme) and the relative amount of collagen per protein was analysed.
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No products found
because this supplier's products are not listed.
Martin H. Berryer, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Starting on day 2 human induced neural progenitor-like cells were harvested with Accutase (Innovative Cell Technology, Inc., AT104-500) and re-plated at 15,000 cells.cm−2 in Astrocyte Medium (ScienCell ...
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No products found
because this supplier's products are not listed.
Kyung-Jin Jang, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
Alpha Glutathione S-Transferase (α-GST): levels were quantified in human model effluent samples from the upper channel using an ELISA kit (DiaPharma). The assay was run following the vendor protocol using a standard curve ranging from 0-64 µg/L ...
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No products found
because this supplier's products are not listed.
Hower Lee, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Rat brain slices were purchased fresh-frozen (Zyagen), kept at −80 until use and processed the same way as chicken and mouse sections.
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No products found
because this supplier's products are not listed.
Nadya Povysheva, Huiyuan Zheng, Linda Rinaman,
bioRxiv - Neuroscience 2021
Quote:
... adult male Sprague-Dawley rats (n=3; 225-250 g BW) were anesthetized by isoflurane inhalation (1-3% in oxygen; Halocarbon Laboratories) and placed into a stereotaxic device in the flat skull position ...
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No products found
because this supplier's products are not listed.
Y Vicioso, et al.,
bioRxiv - Immunology 2021
Quote:
... and proteins were crosslinked to DNA in two steps: (1) by adding DSG (Disuccinimidyl glutarate; ProteoChem) to a final concentration of 2mM for 45 minutes at room temperature followed by washing once with PBS and (2 ...
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No products found
because this supplier's products are not listed.
Taewon Choi, et al.,
bioRxiv - Bioengineering 2022
Quote:
... rats were stereotactically implanted with five cortical screw electrodes (Fine Science Tools, 19010-00 screws). The implantation coordinates were adopted from a stereotactic atlas [30,31] ...
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No products found
because this supplier's products are not listed.
Alexis Bouin, et al.,
bioRxiv - Microbiology 2022
Quote:
... diluted 1:500 in blocking solution for detection of viral protein VP1 or anti dsRNA antibody (SCICONS, anti-dsRNA mAb J2, 10010500) diluted 1:1000 in blocking solution overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Qianyan Li, et al.,
bioRxiv - Genetics 2022
Quote:
... and the column was washed with lysis buffer to remove unbound proteins before eluting the proteins with 50 μM biotin (Chem-Impex Int’l) in low salt buffer (20mM HEPES-KOH pH 7.2 ...
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No products found
because this supplier's products are not listed.
Deborah L. Gater, et al.,
bioRxiv - Biophysics 2022
Quote:
... Vitamin D binding protein (DBP) was purchased from Athens Research and Vitamin D Binding protein (VDR ...
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No products found
because this supplier's products are not listed.
Xavier Martiáñez-Vendrell, et al.,
bioRxiv - Microbiology 2019
Quote:
... vivax pLDH proteins expressed in insect cells (3001, ReliaTech GmbH, Germany) were used as reference material ...
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No products found
because this supplier's products are not listed.
Beatriz Gil, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Recombinant alpha- synuclein protein was a gift from rPeptide (GA, USA).
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No products found
because this supplier's products are not listed.
Alicia Kairouani, et al.,
bioRxiv - Plant Biology 2023
Quote:
... proteins were extracted using a Constant cell disrupter system (Constant Systems) with a disruption pressure of 2.35kbar and then purified using His-bind buffer kit (Millipore ...
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No products found
because this supplier's products are not listed.
Yirui Li, Julie A. Champion,
bioRxiv - Bioengineering 2024
Quote:
OVA protein vesicles were imaged by a TEM (JEM 100CX-II, JEOL). 5 μL of protein vesicle solution was dropped on a copper grid (Electron Microscopy Sciences ...
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Cat# G209,
USD $10.00/EA
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Samantha Sarni, et al.,
bioRxiv - Biochemistry 2020
Quote:
... purified Gag proteins were dialyzed (3500 MWCO dialysis tubing, BioDesign Inc. of New York) into 500 mM ammonium acetate ...
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No products found
because this supplier's products are not listed.
Jie Hu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Total protein was extracted from cells using radio immunoprecipitation assay Lysis Buffer (CoWin Biosciences, Beijing, China) containing 1 mM phenylmethylsulfonyl fluoride (Beyotime ...
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No products found
because this supplier's products are not listed.
Katy R. McCarron, et al.,
bioRxiv - Cell Biology 2023
Quote:
... anti-LC3 (1:200 WB, 1:200 IF, Nanotools; 5F10), anti-p62 (1:1,000 WB ...
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No products found
because this supplier's products are not listed.
Hammam Antar, et al.,
bioRxiv - Biochemistry 2021
Quote:
... CTP/parSDNA 40 pre-mix (10 μL) was added to protein solution (10 μL) using BenchSmart 96 (Rainin) dispenser robot and mixed by pipetting ...
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No products found
because this supplier's products are not listed.
Pooja Kesari, et al.,
bioRxiv - Biophysics 2021
Quote:
... PEG 8000) with 0.4 µl protein + 0.4 µl reservoir as sitting drops in 96-well XJR trays (Rigaku Reagents) at 14°C ...
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No products found
because this supplier's products are not listed.
Michael Adams, et al.,
bioRxiv - Biochemistry 2021
Quote:
... with peptides generated from roughly 500ng of proteins injected for each analysis onto either 75 micron x 25cm packed emitter columns (New Objective) or 75 micron x 50 cm C18 Acclaim Pepmap columns (Thermo Scientific) ...
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No products found
because this supplier's products are not listed.
Wenwei Li, et al.,
bioRxiv - Microbiology 2021
Quote:
... and 1 mM dithiothreitol) and 50 μL of 1 mM D-luciferin potassium salt (Prolume). The neutralization half-maximal inhibitory dilution (IC50 ...
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No products found
because this supplier's products are not listed.
Akul Singhania, et al.,
bioRxiv - Immunology 2021
Quote:
... 1% penicillin/streptomycin (Omega Scientific, Tarzana, CA), and 50 U/ml Benzonase (Millipore Sigma ...
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No products found
because this supplier's products are not listed.
Thorben Schramm, Vanessa Pahl, Hannes Link,
bioRxiv - Systems Biology 2023
Quote:
... covered with Breathe-Easy (Diversified Biotech BEM-1) adhesive membrane ...
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No products found
because this supplier's products are not listed.
Tao Zhang, et al.,
bioRxiv - Plant Biology 2022
Quote:
... and 1/16” stainless steel tubing were acquired from IDEX Health & Sciences (Oak Harbor ...