-
No products found
because this supplier's products are not listed.
Lukas-Adrian Gurzeler, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Lysates were reduced with 10 mM DTT (Indofine Chemicals) at 30°C for 30 min ...
-
No products found
because this supplier's products are not listed.
James S. Dhaliwal, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cleared lysates were then incubated with α8-oxoG (QED Bioscience) at 4°C for 1 hr to capture ORBs ...
-
No products found
because this supplier's products are not listed.
Steven J. Grzegorski, et al.,
bioRxiv - Genetics 2019
Quote:
... Prothrombin levels in the expression media and the cell lysate were then quantified by ELISA using a matched-pair antibody set ELISA kit (Affinity Biologicals Inc.; FII-EIA).
-
No products found
because this supplier's products are not listed.
Emily S. Norton, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... lysates were clicked to 10 μM DBCO-S-S-PEG3-biotin (BroadPharm) for 6 hours at room temperature ...
-
No products found
because this supplier's products are not listed.
Maayan Karlinski Zur, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... The lysates were transferred into soft tissue homogenizing CK14 tubes (Bertin Corp), placed in a homogenizer shaker at 400bps for 2 sec ...
-
No products found
because this supplier's products are not listed.
Angelica F. Castañeda, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The clarified lysate was filtered through a 0.45 µm PES filter (Foxx Life Sciences). The protein was purified on an equilibrated HisTrap (GE Healthcare ...
-
No products found
because this supplier's products are not listed.
Julika Radecke, et al.,
bioRxiv - Neuroscience 2022
Quote:
... the rat synaptosome grids were mounted in a cryo-holder (Gatan, Pleasonton, CA, USA) and transferred to a Tecnai F20 (FEI ...
-
No products found
because this supplier's products are not listed.
Ipsita Pal, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... The cleared lysates were incubated with pre-washed GTP-sepharose or m7-GTP-sepharose beads (AXXORA) with an additional CAP lysis buffer and incubated for 3hrs at 4 °C with occasional shaking ...
-
No products found
because this supplier's products are not listed.
Taewon Choi, et al.,
bioRxiv - Bioengineering 2022
Quote:
... rats were stereotactically implanted with five cortical screw electrodes (Fine Science Tools, 19010-00 screws). The implantation coordinates were adopted from a stereotactic atlas [30,31] ...
-
No products found
because this supplier's products are not listed.
Michael H Jones, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The primary antibody was visualized using Simple Mouse Stain MAX-PO (Rat) (Nichirei Biosciences, Tokyo, Japan) for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Ido Lavi, et al.,
bioRxiv - Microbiology 2023
Quote:
The following antibodies were used for immunofluorescence studies: Rat anti LANA (Advanced Biotechnologies, cat#13-210-100), Mouse anti LANA (Leica ...
-
No products found
because this supplier's products are not listed.
N.D. Maxwell, et al.,
bioRxiv - Neuroscience 2023
Quote:
... rats were moved to the microdialysis room and fitted with probes from BASi (1 mm, MD-2202) into the ARC targeted cannula and perfused with artificial cerebral spinal fluid (150 mM NaCl ...
-
No products found
because this supplier's products are not listed.
Linyuan Shi, et al.,
bioRxiv - Neuroscience 2023
Quote:
... rats in the ethanol only group (EtOH) were injected with vehicle and drank 10% ethanol (Decon Laboratories) mixed with 0.1% saccharin ...
-
No products found
because this supplier's products are not listed.
Carina C D Joe, et al.,
bioRxiv - Bioengineering 2021
Quote:
... the clarified lysate was concentrated by tangential flow filtration at 5 L/min/m2 using a KR2i KrosFlo (Repligen) with Pellicon 2 mini cassettes (300 kDa ...
-
No products found
because this supplier's products are not listed.
F. Esra Demircioglu, et al.,
bioRxiv - Biochemistry 2019
Quote:
... The lysate was immediately mixed with 0.1 M phenylmethanesulfonyl fluoride (PMSF) (50 μl per 10 ml lysate) and 250 units of TurboNuclease (Eton Bioscience), and cleared by centrifugation ...
-
No products found
because this supplier's products are not listed.
Nadya Povysheva, Huiyuan Zheng, Linda Rinaman,
bioRxiv - Neuroscience 2021
Quote:
... adult male Sprague-Dawley rats (n=3; 225-250 g BW) were anesthetized by isoflurane inhalation (1-3% in oxygen; Halocarbon Laboratories) and placed into a stereotaxic device in the flat skull position ...
-
No products found
because this supplier's products are not listed.
Anna M.R. Hayes, et al.,
bioRxiv - Physiology 2022
Quote:
Juvenile male and female rats (n=9 per sex) were given daily amounts of acesulfame potassium (ACE-K group; catalog # A2815, Spectrum Chemical, Gardena ...
-
No products found
because this supplier's products are not listed.
Bart C.J. Dirven, et al.,
bioRxiv - Neuroscience 2024
Quote:
... incubation of the primary antibodies was performed overnight (guinea pig anti-cFos, 1:750, 226004, Synaptic Systems; rat anti-RFP, 1:1000, 5f8, Chromotek; mouse anti-5mC, 1:500, GWB-BD5190, GenWay Biotech ...
-
No products found
because this supplier's products are not listed.
Salwa Sebti, Zhongju Zou, Michael U. Shiloh,
bioRxiv - Physiology 2022
Quote:
... Cleared lysates were diluted in 2× SDS-PAGE loading buffer and submitted to western blotting using anti-p62 (GP62-C, Progen, 1:1,000 dilution), anti-LC3B (L7543 ...
-
No products found
because this supplier's products are not listed.
Alia Hasan, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Serum PTH or PTH secreted to growth medium in organ cultures was measured using rat or mouse 1–84 Intact PTH ELISA kit (Quidel, Athens, Ohio).
-
No products found
because this supplier's products are not listed.
Wedad Alhassen, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The slices were then incubated with rabbit anti-rat adenylate cyclase 3 (ADCY3, RPCA-ACIII; Encor Biotechnology, Alachua, FL, USA; 1:5000) and goat anti-human MCHR1 (goat anti-human MCHR1 ...
-
No products found
because this supplier's products are not listed.
Jessica Corry, et al.,
bioRxiv - Biochemistry 2022
Quote:
... cells were single cell sorted using the CellenONE (Scienion) into 96 well plates in 20% conditioned media ...
-
No products found
because this supplier's products are not listed.
William Tyler Frantz, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cells from BMP-KO samples as well as non-melanocyte lineage keratinocytes were then removed ...
-
No products found
because this supplier's products are not listed.
Jeanette M. Metzger, et al.,
bioRxiv - Neuroscience 2022
Quote:
GFP-expressing human embryonic kidney cells (i.e., GFP-HEK cells, GenTarget Inc.) were used as an RNP delivery cell model ...
-
No products found
because this supplier's products are not listed.
Sanam Shafaatalab, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The cells were counted using the CellDrop automated cell counter (Froggabio, ON). The lysis buffer used contained 100 mM HEPES (pH= 8-8.5 ...
-
No products found
because this supplier's products are not listed.
Ni Zeng, et al.,
bioRxiv - Immunology 2021
Quote:
... Splenocytes depleted of T cells were used as antigen-presenting cells (APCs)/feeder cells and were irradiated in RS2000 (Rad Source Technologies) with a total dose of 30 Gy within a period of 10 mins ...
-
No products found
because this supplier's products are not listed.
Ragna S. Häussler, et al.,
bioRxiv - Biochemistry 2019
Quote:
... All secreted proteins were produced by using the transient Icosagen Cell Factory system with CHOEBNALT-85 cells and the QMCF Technology (Icosagen Cell Factory OÜ, Tartu, Estonia). Cells were maintained in a 50:50 mixture of 293 SFM II (Gibco ...
-
No products found
because this supplier's products are not listed.
Zixuan Liu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Cell counting kit-8 (M4839, AbMole).
-
No products found
because this supplier's products are not listed.
K.G. Daniels, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... T cells were cryopreserved in RPMI1640 (UCSF cell culture core) with 20% human AB serum (Valley Biomedical, #HP1022HI) and 10% DMSO ...
-
No products found
because this supplier's products are not listed.
Amanda Acosta-Ruiz, et al.,
bioRxiv - Bioengineering 2019
Quote:
HEK 293T cells (authenticated by Bio-Synthesis, Inc ...
-
No products found
because this supplier's products are not listed.
Jen M. Hope, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
PC12 cells (NeuroScreen-1 subclone, Cellomics, discontinued) were maintained in complete medium (F12K supplemented with 15% horse serum and 2.5% FBS ...
-
No products found
because this supplier's products are not listed.
Akiko Ogawa, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... cells were incubated with urantide (Peptide Institute) for 30 min at 100 nM (for UTS2R inhibition) ...
-
No products found
because this supplier's products are not listed.
Oihana Iriondo, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... cells were moved to hypoxic incubators (Nuaire NU-5831 for 4% O2 ...
-
No products found
because this supplier's products are not listed.
Sumeet Nayak, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... spotted (2.5μl of 105 cells/ml PBS cell suspension) and lysed on positively charged microscope slides (Globe Scientific, #1358W) by 7.5μl spreading buffer (0.5%SDS ...
-
No products found
because this supplier's products are not listed.
Shisu Zhao, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Cell counting kit 8 (CCK8) experiment for cell viability assay were performed using the CCK8 Kit (TargetMol, MA, USA). After a treatment ...
-
No products found
because this supplier's products are not listed.
Juline Poirson, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Cells were treated with 1 µM MLN4924 (Chemietek) for 24 hours ...
-
No products found
because this supplier's products are not listed.
Mike R. Schlabach, et al.,
bioRxiv - Immunology 2023
Quote:
... T cells were electroporated with Cas9 mRNA (Trilink) and expanded inn 10U human IL-2 in X-VIVO-15 media for 10 additional days ...
-
No products found
because this supplier's products are not listed.
Rosalia Paterno, et al.,
bioRxiv - Neuroscience 2024
Quote:
Concentrated GFP+ MGE cells (∼600 cells per nl) were injected using 30° beveled glass micropipettes (80 μm diameter tip, Witerol 5 μl, Drummond Scientific) prefilled with mineral oil into cortex or hippocampus of recipient pup (P2-4) ...
-
No products found
because this supplier's products are not listed.
Richard Gerum, et al.,
bioRxiv - Biophysics 2022
Quote:
... 1 ml of alginate solution are then added to the cell pellet of 106 cells in the Falcon tube and mixed using a positive displacement pipette (15314274, Gilson/Fisher Scientific) by slowly (∼ 2 s cycle time ...
-
No products found
because this supplier's products are not listed.
Daniel S Saxton, Jasper Rine,
bioRxiv - Molecular Biology 2019
Quote:
... RFP-expressing cells were diluted and plated at a density of ∼10 cells/plate (CSM-Trp (Sunrise Science Products, San Diego, CA), 1% agar) ...
-
No products found
because this supplier's products are not listed.
Allison J. Greaney, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cells were stained with recombinant biotinylated ACE2 (ACROBiosystems, AC2-H82E6) and serial dilutions of rCR3022 antibody for 1 h at room temperature ...
-
No products found
because this supplier's products are not listed.
Hannah Weber, Rachel Ruoff, Michael Garabedian,
bioRxiv - Cancer Biology 2019
Quote:
... cells were double cross-linked with DSG (ProteoChem; cat # c1104) for 20 min and 1% formaldehyde for 10 min ...
-
No products found
because this supplier's products are not listed.
Stephen K. Dolan, et al.,
bioRxiv - Microbiology 2023
Quote:
... the cells were grown in Turbo Broth medium (Molecular Dimensions) (1 L ...
-
No products found
because this supplier's products are not listed.
Oded Danziger, et al.,
bioRxiv - Microbiology 2021
Quote:
... All cell lines were routinely tested (Boca Scientific #50-168-5641) negative for Mycoplasma contamination ...
-
No products found
because this supplier's products are not listed.
Martin P. Schwalm, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... was added to the cells using an Echo acoustic dispenser (Labcyte) and the cells were incubated additional 20h at 37 °C/5 % CO2 ...
-
No products found
because this supplier's products are not listed.
Keng Hou Mak, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and were maintained in mesothelial cell growth medium (MSO-1, Zen-Bio, Inc.). Human cervical adenocarcinoma cell line HeLa from American Type Culture Collection was maintained in DMEM medium supplemented with 10% fetal bovine serum ...
-
No products found
because this supplier's products are not listed.
Tugsan Tezil, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Histones were extracted from HEK293T cells by EpiQuik Total Histone Extraction Kit (Epigentek), following manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Elisa Matas-Rico, et al.,
bioRxiv - Cancer Biology 2021
Quote:
T cell migration was measured using 48-well chemotaxis chambers (Neuro Probe, Inc.) equipped with 5 µm-pore polycarbonate membranes (8 µm-pore for melanoma cells) ...
-
No products found
because this supplier's products are not listed.
Louis J. Born, et al.,
bioRxiv - Bioengineering 2021
Quote:
... The cell monolayer was then scratched using a 200 μL pipette tip (Rainin, 30389243). Media was then replaced using the same serum starving conditions ...
-
No products found
because this supplier's products are not listed.
Li Sun, et al.,
bioRxiv - Cell Biology 2022
Quote:
... cells were treated with 5μM ATP analog 3-BrB-PP1 (Toronto Research Chemicals; A602985) during 18 °C incubation for 1.5 hr to inactivate Sty1 kinase activity before being collected ...