-
No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
-
No products found
because this supplier's products are not listed.
Saptarshi Sinha, et al.,
bioRxiv - Immunology 2022
Quote:
... To block non-specific protein binding either 2.5% goat or 2.5% horse serum (Vector Laboratories, Burlingame ...
-
No products found
because this supplier's products are not listed.
Krystallenia Paniskaki, et al.,
bioRxiv - Immunology 2022
Quote:
... the complete sequence of B.1.1.7 D614G Spike mutant (alpha) (JPT Peptide Technologies) or wildtype (WT) S-protein (Miltenyi Biotec) or left untreated as a control for 16 h ...
-
No products found
because this supplier's products are not listed.
Catarina Nascimento, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and after two washing steps with PBS the nonspecific binding of immunoglobulins was prevented by incubating the tissue slices with the Protein Block Novocastra Solution (Leica Biosystems) for 60 min ...
-
No products found
because this supplier's products are not listed.
How-Wing Leung, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Total protein was isolated using an RNA-protein extraction kit (Macherey-Nagel), as specified by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Hui Chen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The proteins were first purified using CaptivA Protein A affinity resin (Repligen), unless otherwise specified ...
-
No products found
because this supplier's products are not listed.
Laurie Arnaud, et al.,
bioRxiv - Neuroscience 2021
Quote:
... as human recombinant proteins (Peprotech). APOE3 was resuspended in 20 mM Sodium Phosphate ...
-
No products found
because this supplier's products are not listed.
David Gonzalez-Perez, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Proteins were separated by SDS-PAGE (12% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and transferred to PVDF membranes using an iBlot2 Gel Transfer Device (Thermo Fisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Aneta Mikulasova, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... as well as the super-enhancer proteins BRD4 and MED1 (Bethyl, Montgomer, TX, USA), and the transcription factor MYC (Santa Cruz Biotechnology ...
-
No products found
because this supplier's products are not listed.
Silvio Schmidt, et al.,
bioRxiv - Neuroscience 2020
Quote:
... the S100 calcium-binding protein B (S100B; rabbit, Synaptic system), and BrdU (mice ...
-
No products found
because this supplier's products are not listed.
Takafumi Nishida, et al.,
bioRxiv - Physiology 2023
Quote:
... anti-rabbit anti-sterol regulatory element-binding protein-2 (SREBP2, Cayman chemical, MI), anti PCSK9 (Cayman chemical) ...
-
No products found
because this supplier's products are not listed.
Chen Su, Yuxin Wang, Xiaoke Yang, Junyu Xiao,
bioRxiv - Biochemistry 2023
Quote:
... The bound proteins were eluted with the binding buffer supplemented with 10 mM desthiobiotin (IBA Lifesciences). The samples were analyzed by SDS-PAGE and detected by immunoblotting using antibodies to the strep tag (1:3,000 ...
-
No products found
because this supplier's products are not listed.
Tian Yu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... non-specific protein binding was blocked in a medium consisting of 2% normal donkey serum (Jackson ImmunoResearch) in antibody medium (AB medium ...
-
No products found
because this supplier's products are not listed.
Jean-Philippe Guégan, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... The level of protein binding to DNA consensus sequence was then assessed by ELISA using the TransAM NF-kB Family kit (Active Motif) according to manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Lorna Cryan, et al.,
bioRxiv - Biochemistry 2019
Quote:
... matrix protein (Rockland Immunochemicals ...
-
No products found
because this supplier's products are not listed.
Kai Chen, et al.,
bioRxiv - Bioengineering 2023
Quote:
... RNPs were further complexed with Alt-R Cas9 electroporation enhancer (IDT, 100-nt ssDNA) with a 1:1 mole ratio of enhancer to RNP in supplier-recommended buffers (Lonza). For LNP delivery ...
-
No products found
because this supplier's products are not listed.
Brett Stern, Peter Monteleone, Janet Zoldan,
bioRxiv - Bioengineering 2022
Quote:
Viral spike protein (either SARS-CoV-2 Spike Protein, S1 Subunit, RayBiotech, or MERS-CoV-1 Spike Protein, S1 Subunit, BioVision) was added to endothelial culture media of CD34+ iPSC-EP-laden hydrogels at a concentration of 10 μg/mL either one or five days after cell encapsulation ...
-
No products found
because this supplier's products are not listed.
Ana R. López-Pérez, et al.,
bioRxiv - Genomics 2021
Quote:
... Dynal Protein A magnetic beads (Diagenode) and ChIP-graded anti-myc antibody (ab9132 ...
-
No products found
because this supplier's products are not listed.
Merve Sen, et al.,
bioRxiv - Cell Biology 2021
Quote:
... we used heterozygous P23H rats obtained by crossing homozygous RHOP23H rats with RHOWT rats (CDH IGS Rat; Charles River, Germany). Animals were housed in the Institute for Ophthalmic Research animal facility under standard white cyclic lighting ...
-
No products found
because this supplier's products are not listed.
Jessica Corsi, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... followed by the addition of protein in Alpha Buffer using the EL460 dispenser (BioTek). After 15 min of incubation ...
-
No products found
because this supplier's products are not listed.
Isabel Mogollón, et al.,
bioRxiv - Cell Biology 2020
Quote:
... containing several copies of TCF/Lef1 DNA binding sites driving expression of the H2B-EGFP fusion protein (#013752; Jackson Laboratories); FGF20βGal mice have an Fgf20-β-galactosidase (βGal ...
-
No products found
because this supplier's products are not listed.
Mericien Venzon, et al.,
bioRxiv - Microbiology 2022
Quote:
... and lipopolysaccharide (LPS)-binding protein were quantified in the plasma by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (MyBioSource, CA).
-
No products found
because this supplier's products are not listed.
Olivier Thouvenin, et al.,
bioRxiv - Biophysics 2019
Quote:
... and alpha-bungarotoxin (TOCRIS) were injected in the center of the diencephalic ventricle in order to label CSF and paralyze the fish in a single injection ...
-
No products found
because this supplier's products are not listed.
Jürgen G. Okun, et al.,
bioRxiv - Physiology 2020
Quote:
... or protein-enriched (80%E protein, 10% carbohydrate, 10% fat; D16080303, Research Diets, USA) diets for a period of 3 weeks with various metabolic phenotyping tests ...
-
No products found
because this supplier's products are not listed.
Fransky Hantelys, et al.,
bioRxiv - Cell Biology 2019
Quote:
Binding analyses were performed with recombinant protein VASH1 (Abnova H00022846-P01) at 100 µg/ml over the immobilized FGF1 ...
-
No products found
because this supplier's products are not listed.
Supawadee Umthong, et al.,
bioRxiv - Microbiology 2020
Quote:
... rat anti-MLV transmembrane protein/p15E (clone 42/114, Kerafast), rabbit anti-myc (Cell Signaling Technology) ...
-
No products found
because this supplier's products are not listed.
Chengbao Ma, et al.,
bioRxiv - Microbiology 2023
Quote:
Protein binding kinetics was analyzed using BLI assays with the Octet RED96 instrument (Molecular Devices) following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Meagan N. Esbin, et al.,
bioRxiv - Cell Biology 2024
Quote:
... + 1:1000 Enhancer (Biotium) and incubated for ∼10-15min before starting imaging ...
-
No products found
because this supplier's products are not listed.
Dhaval Dixit, et al.,
bioRxiv - Immunology 2023
Quote:
... or Fas ligand + Enhancer (Enzo Life Sciences) for 4 hours in a 37°C 5% CO2 incubator ...
-
No products found
because this supplier's products are not listed.
Belen Esquerra-Ruvira, et al.,
bioRxiv - Molecular Biology 2022
Quote:
NZYBlue Protein Marker (NZYtech) was used for protein size estimation and protein concentrations were measured with QUBIT® 2.0 (Invitrogen).
-
No products found
because this supplier's products are not listed.
He Liu, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
The cytoplasmic and nucleic protein were extracted separately using the protein extraction kit (Boster) according to the manufacture’s introduction ...
-
No products found
because this supplier's products are not listed.
Jingqi Zhou, et al.,
bioRxiv - Genomics 2021
Quote:
Zebrafish’s bone morphogenetic protein (BMP) and bone morphogenetic protein receptor genes were used as query to search the BMP and BMP receptor gene families in P ...
-
No products found
because this supplier's products are not listed.
Laurens H. Lindenburg, et al.,
bioRxiv - Biochemistry 2020
Quote:
... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Coraline Chéneau, et al.,
bioRxiv - Immunology 2020
Quote:
... recombinant protein and CD14 antibody (Beckman) were used at 20 μg/ml ...
-
No products found
because this supplier's products are not listed.
Ying He, et al.,
bioRxiv - Immunology 2023
Quote:
The following antibodies were used for labeling surface markers: rat anti-mouse CD16/32 (block the nonspecific binding to Fc receptors, clone:2.4G2, cat#:BE0307, Bio X Cell, West Lebanon, NH), rat anti-mouse Lineage cocktail (conjugated with Pacific blue ...
-
No products found
because this supplier's products are not listed.
Guojun Wu, et al.,
bioRxiv - Microbiology 2022
Quote:
... lipopolysaccharide-binding protein (Hycult Biotech, PA, USA), leptin (P&C ...
-
No products found
because this supplier's products are not listed.
Noémie Aurine, et al.,
bioRxiv - Cell Biology 2019
Quote:
... or universal IFN-type (a recombinant human IFN-alpha protein hybrid; Pbl Assay Science, 11200-2). Bat cells seeded in 12-well plates at 80% confluence were treated for 6 h at 37°C with either poly IC (0–1000 ng/mL ...
-
No products found
because this supplier's products are not listed.
Gregory P. Williams, et al.,
bioRxiv - Immunology 2024
Quote:
... incubated with fluorescence enhancer (Mabtech), and air-dried for reading ...
-
No products found
because this supplier's products are not listed.
Tim Rick, et al.,
bioRxiv - Microbiology 2021
Quote:
To verify the functionality of mutated SoGGDEF proteins they were tested for GTP binding using MANT-GTP (Jena Bioscience, Germany). Proteins were diluted to 1 μM and 100 μl of each sample was added in triplicates into a white round bottom 96-well plate before MANT-GTP with a final concentration of 1 μM was added ...
-
No products found
because this supplier's products are not listed.
Tshegofatso Ngwaga, et al.,
bioRxiv - Microbiology 2023
Quote:
Protein carbonyls were visualized using the OxiSelect Protein Carbonyl Immunoblot Kit (Cell Biolabs). Tnrf1-/- BMDMs were seeded in 24-well plates at 2.5x105 one day prior to infection with indicated L ...
-
No products found
because this supplier's products are not listed.
Seren Hamsici, Gokhan Gunay, Handan Acar,
bioRxiv - Bioengineering 2022
Quote:
... Fmoc protected amino acids (Gyros Protein Technologies) were removed through treatment with 20% piperidine/DMF solution for 45 min (three times for 15 min ...
-
No products found
because this supplier's products are not listed.
Li Wang, et al.,
bioRxiv - Microbiology 2022
Quote:
... The expression of nucleocapsid protein was confirmed by the SARS-CoV-2 Nucleocapsid Protein ELISA Kit (ABclonal, Woburn, MA) and the cell clone supporting the most efficient virus rescue was selected (VeroE6-N) ...
-
With the RapidClean protein removal kit, completely remove protein from aqueous solutions of...
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Ethan L. Morgan, et al.,
bioRxiv - Microbiology 2020
Quote:
... Proteins were detected using WesternBright ECL (Advansta, USA) and visualised on X-ray film.
-
No products found
because this supplier's products are not listed.
Kevin Menden, et al.,
bioRxiv - Neuroscience 2022
Quote:
... gene body and enhancer regions we have used the MethylationEPIC bead chip arrays (Illumina). Bisulfite conversion of genomic DNA ...
-
No products found
because this supplier's products are not listed.
Chunyan Wang, et al.,
bioRxiv - Microbiology 2020
Quote:
... Antibody binding to the spike proteins was determined using a 1:2000 diluted HRP conjugated goat anti-human IgG (ITK Southern Biotech) for 1 h at RT and tetramethylbenzidine substrate (BioFX) ...
-
No products found
because this supplier's products are not listed.
Laurice Flowers, et al.,
bioRxiv - Microbiology 2022
Quote:
... Protein precipitation reagent (Lucigen) was added and samples were spun at maximum speed ...
-
No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... or C1s proteins (Complement Technologies), or BSA (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Alba Ortega-Gascó, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Silver enhancement (Aurion R-gent Silver enhancer kit, Electron Microscopy Sciences) was performed following the manufacturer’s directions ...
-
No products found
because this supplier's products are not listed.
Sophie L. Lewandowski, et al.,
bioRxiv - Cell Biology 2020
Quote:
... with an alpha plate reader (TECAN Spark). After the perifusion ...
-
No products found
because this supplier's products are not listed.
Kun Wang, et al.,
bioRxiv - Biochemistry 2022
Quote:
... To reconstitute MBOAT7 proteins in PMAL-C8 (Anatrace), MBOAT7 in detergent was mixed with PMAL-C8 at a 1:3 (w:w ...