-
No products found
because this supplier's products are not listed.
Du-Hwa Lee, et al.,
bioRxiv - Plant Biology 2022
Quote:
... RealHelixTM qPCR kit (NANOHELIX; Korea), and the StepOnePlus Realtime PCR System (Applied Biosystems ...
-
No products found
because this supplier's products are not listed.
Brian W. Ji, et al.,
bioRxiv - Microbiology 2019
Quote:
... inverted sterile 200 µL pipette tips (Rainin RT-L200F) were used to core out a small sample from the stool ...
-
No products found
because this supplier's products are not listed.
George C. Russell, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... Purified RNA was treated with RTS DNase (Cambio, Cambridge, UK) before cDNA synthesis.
-
No products found
because this supplier's products are not listed.
Matteo Chighizola, et al.,
bioRxiv - Biophysics 2022
Quote:
... for 30 min at RT) glass-bottomed plates (Willco Wells). The fixed cells were characterised using a Bioscope Catalyst AFM from Bruker ...
-
No products found
because this supplier's products are not listed.
Joel D. Pearson, et al.,
bioRxiv - Microbiology 2020
Quote:
The 2019-nCoV TaqMan RT-PCR Kit from Norgen Biotek and 2019-nCoV ...
-
No products found
because this supplier's products are not listed.
Leanne E. Wybenga-Groot, C. Jane McGlade,
bioRxiv - Biochemistry 2020
Quote:
... 10% (w/v) PEG8000 at rt using a Mosquito robot (TTP Labtech). Small rod-like crystals were observed after approximately five months ...
-
No products found
Ons Mamai, et al.,
bioRxiv - Cell Biology 2022
Quote:
... After blocking the membrane for 1h at RT with AdvanBlock (Advansta®), and proteins were detected by incubation with relevant antibodies (Supplementary Table S6 ...
-
No products found
because this supplier's products are not listed.
Megan M. Sperry, et al.,
bioRxiv - Bioengineering 2023
Quote:
... 12.5-minute RT window and 25 ppm XIC width in Peakview (Sciex). The reported quantification for a protein is the sum of all the correctly integrated peptides (FDR<0.05 ...
-
No products found
because this supplier's products are not listed.
Annu Nummi, et al.,
bioRxiv - Cell Biology 2021
Quote:
... DAB (High Contrast DAB, BiositeHisto Nordic Biosite cat. no BCB-R7IKBJ, 10min, RT), wash ...
-
No products found
because this supplier's products are not listed.
Kyung Ah Han, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Whole-cell configuration was generated at RT using MPC-200 manipulators (Sutter Instrument) and a Multiclamp 700B amplifier (Molecular Devices) ...
-
No products found
because this supplier's products are not listed.
Valeria Scagliotti, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... samples were incubated at room temperature (RT) with Histoclear II (National Diagnostics, HS202) (2 x 20 minutes for E9.5-E11.5 and postnatal pituitaries ...
-
No products found
Troy T. Rohn, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The RNA extraction and qPCR were performed by Creative Biogene (Shirley, NY, USA).
-
No products found
because this supplier's products are not listed.
Saptarshi Mallick, et al.,
bioRxiv - Physiology 2022
Quote:
... 2X TaqMan Universal Master Mix (Applied Biosystems, TaqMan® Gene Expression Systems), and cDNA template ...
-
No products found
because this supplier's products are not listed.
Alex Steimle, et al.,
bioRxiv - Microbiology 2023
Quote:
... samples were shaked vigorously for 5 minutes using a Shake Master NEO (BioSpec Products ...
-
No products found
because this supplier's products are not listed.
DaNae R. Woodard, et al.,
bioRxiv - Biochemistry 2022
Quote:
... followed by incubation in developing buffer (30 min at RT, G-Biosciences, St. Louis, MO), and again in fresh developing buffer overnight at 37°C with gentle shaking ...
-
No products found
because this supplier's products are not listed.
Huanhuan Li, et al.,
bioRxiv - Biochemistry 2021
Quote:
... pcDNA-NP and pPOLI-NA-RT plasmid encoding neuraminidase (NA) vRNA segment using PEI (Polyscience) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Jan Zlamal, et al.,
bioRxiv - Immunology 2022
Quote:
... a calcium independent ligand of PS (200nM, 15 min, RT; Haematologic Technologies, Essex Junction, USA).29,30 Sufficient blocking concentrations of Lactadherin were assessed via Calibrated Automated Thrombogram (CAT ...
-
No products found
because this supplier's products are not listed.
Alexia Carré, et al.,
bioRxiv - Immunology 2023
Quote:
... INS KO was validated by RT-PCR and by INS ELISA (Mercodia, #10-1113-01) according to the manufacturer’s protocol.
-
No products found
because this supplier's products are not listed.
R. Doolan, et al.,
bioRxiv - Immunology 2024
Quote:
... and reverse transcribed using FIREScript RT cDNA synthesis Kit Solis Biodyne (Lucerna 06 −15-00200) for qPCR analysis ...
-
No products found
because this supplier's products are not listed.
Cécile Thirant, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Cells were monthly checked by qPCR (Venor® GeM qEP 11-9250, Minerva biolabs®) for the absence of mycoplasma.
-
No products found
because this supplier's products are not listed.
Veronica Ferrucci, et al.,
bioRxiv - Genetics 2020
Quote:
... Real-time RT-PCR was performed using ‘RealQquality RQ-2019-nCoV’ kits (Ab Analitica; IVD approved) for detection of the RdRp and E genes (from the SarsCov2 N gene) ...
-
No products found
because this supplier's products are not listed.
Hima Priyanka Nadimpalli, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The RT-Biolumicorder (Lesa-Technology) consists of a cylindrical cage equipped with a photomultiplier tube (PMT) allowing for recording of real- time longitudinal bioluminescence from single-housed mice ...
-
No products found
because this supplier's products are not listed.
Haizhang Chen, et al.,
bioRxiv - Immunology 2020
Quote:
... pre-treated (2h at RT) with PBS supplemented with varying percentages (v/v) of FCS (PAN Biotech). IgG1 bound to the plates was detected using an HRP-conjugated mouse-anti-human IgG mAb (Jackson ImmunoResearch).
-
No products found
because this supplier's products are not listed.
Stefan J.A. Remmers, et al.,
bioRxiv - Bioengineering 2022
Quote:
... dried overnight at RT and sputter coated with 5 nm gold (Q300TD, Quorum Technologies Ltd, Laughton, UK). Sputter coated constructs were imaged with SEM (Quanta600 ...
-
No products found
because this supplier's products are not listed.
Etkin Akar, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... then long-term drug responses were recorded every 30 minutes through (RT-CES) (ACEA Biosciences; Agilent Technologies). Cell Index values (CI ...
-
No products found
because this supplier's products are not listed.
A. Schlör, et al.,
bioRxiv - Immunology 2022
Quote:
... 1 h incubation at RT) and 1-2 μg/mL of HRP-conjugated secondary antibody (ABIN1981272, antibodies-online) in 50 μL/well supplemented with PBS/1% casein or PBS/5 % NCS for 45 min incubation at RT.
-
No products found
because this supplier's products are not listed.
Florencia Rammauro, et al.,
bioRxiv - Microbiology 2024
Quote:
... Cells were further incubated 1 h at RT with monoclonal antibodies anti-p24 (BLV3, 1/200, VMRD, USA). After three washes with PBS ...
-
No products found
because this supplier's products are not listed.
Gregory Martin, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Triton X-100 and incubation at RT for 60-120 mins and solutions sterile filtered using 0.45 µm Sartopore 2 filters (Sartorius). Triton X-100 inactivated solutions were batch-wise loaded onto a MAb PGT145 immunoaffinity column for capture of ConM and ConS Env trimers ...
-
No products found
because this supplier's products are not listed.
Josée Perreault, et al.,
bioRxiv - Immunology 2020
Quote:
... The plates were incubated once again for one hour at RT followed by washing and addition of 100 μl of 3,3’,5,5’-Tetramethylbenzidine (TMB, ESBE Scientific). The colorimetric reaction proceeded for 20 minutes at RT and was stopped by addition of 100 μl of H2SO4 1N (Fisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Martijn A. Langereis, et al.,
bioRxiv - Microbiology 2021
Quote:
... followed by incubation for 10 minutes at RT with 100 μL/well Super Sensitive TMB (Surmodics, TMBS-1000-01). Reactions were stopped by adding 100 μL/well of 12.5% H2SO4 (Millipore ...
-
No products found
because this supplier's products are not listed.
Harry B. Gristick, et al.,
bioRxiv - Immunology 2022
Quote:
Crystallization screens for IOMA iGL Fab were performed using the sitting drop vapor diffusion method at room temperature (RT) by mixing 0.2 µL Fabs with 0.2 µL of reservoir solution (Hampton Research) using a TTP Labtech Mosquito automatic microliter pipetting robot ...
-
No products found
because this supplier's products are not listed.
Timothy S. Balmer, Laurence O. Trussell,
bioRxiv - Neuroscience 2019
Quote:
... 100-250 μs) using a stimulus generator (Master 8, A.M.P.I.) via a concentric bipolar electrode (CBBPC75, FHC). ChR2 was activated using full-field blue LED light flashes (Lambda TLED+ ...
-
No products found
because this supplier's products are not listed.
Angela Meccariello, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The injection master mix also included preassembled RNP complex of Cas9 protein (200 ng/ml) (PNA Bio)33 ...
-
No products found
because this supplier's products are not listed.
Lisa Stephan, Vicky Tilmes, Martin Hülskamp,
bioRxiv - Molecular Biology 2019
Quote:
... The qPCRs were performed using plates (96 well, 0.2 ml) and cover foil (Opti-Seal Optical Disposable Adhesive) from BIOplastics. Reaction mixtures of 20 µl were composed from 5 µl SYBR Green (Thermo Fisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Kiwon Park, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Quantification of successfully integrated piggyBac transposons was performed using a piggyBac qPCR copy number kit (System Biosciences, PBC100A-1) according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Soyeon Kim, et al.,
bioRxiv - Cell Biology 2019
Quote:
... or fixed cells (Supplemental Figures S1D and S6A) were acquired on an Applied Precision Deltavision RT microscope equipped with CoolSnap-HQ (Photometrics) CCD camera with a 60 X objective ...
-
No products found
because this supplier's products are not listed.
Anton Sumser, et al.,
bioRxiv - Neuroscience 2022
Quote:
... at room temperature (RT) with constant agitation and subsequently with the primary antibody (Rabbit anti somatostatin, 1:500, BMA Biomedicals; Rabbit anti parvalbumin ...
-
No products found
because this supplier's products are not listed.
Merve Avar, et al.,
bioRxiv - Neuroscience 2020
Quote:
The reaction buffer of the RT-QuIC consisted of HaPrP23-231 filtered using 100 kDa centrifugal filters (Pall Nanosep OD100C34) at a concentration of 0.1 mg/mL ...
-
No products found
because this supplier's products are not listed.
Susan Paton, et al.,
bioRxiv - Microbiology 2021
Quote:
... RT-PCR was performed using the VIASURE SARS-CoV-2 Real Time PCR Detection Kit (Viasure; CerTest Biotec, Zaragoza, Spain), following the methods provided ...
-
No products found
because this supplier's products are not listed.
Sandra Postić, et al.,
bioRxiv - Physiology 2022
Quote:
... we incubated the slices for 50 minutes at RT in the dye-loading solution (6 μM Calbryte 520, AAT Bioquest), 0.03 % Pluronic F-127 (w/v) ...
-
No products found
because this supplier's products are not listed.
John Paul Tsu Ouyang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Samples were then shaken at 1500 RPM for five minutes at RT in a benchtop shaker (Benchmark Scientific; Model #: H5000-HC). Samples were then incubated for 5 minutes at RT with no shaking ...
-
No products found
because this supplier's products are not listed.
Gunsagar S. Gulati, et al.,
bioRxiv - Cell Biology 2019
Quote:
... red blood cells were depleted with ACK lysis buffer by incubating at RT for 10 min and Fc-blocked by incubating with rat IgG (LifeSpan BioSciences) for 10 min ...
-
No products found
because this supplier's products are not listed.
A Elgheznawy, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Platelets were activated with appropriately diluted agonists for 8 min at 37°C followed by 8 min at RT in the presence of saturating amounts of PE-coupled JON/A (4H5, Emfret Analytics) detecting activated αIIbβ3 integrin and FITC-coupled anti-P-selectin (Wug.E9 ...
-
No products found
because this supplier's products are not listed.
Ester Orav, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Sections were incubated for 24-72 hrs at RT with rabbit anti-5-HT primary antibody (1:1000, cat no 20080, Immunostar, USA) in carrier solution containing 1% NGS ...
-
No products found
because this supplier's products are not listed.
Ryan Finethy, et al.,
bioRxiv - Immunology 2020
Quote:
... and cells were blocked with PBS containing 5% Bovine Serum Albumin (BSA) for 30 min at RT Cells were stained with rabbit anti-ASC antibody (AG-25B-0006; Adipogen; 1:1000) diluted in blocking buffer overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Aliya Sharipova, et al.,
bioRxiv - Bioengineering 2022
Quote:
... the Fe with 1wt% VH precursor mixture was loaded into a custom-build consolidation die and high-pressure cold-sintered at 2.5 GPa (corresponding to 5 t for 5 mm diameter die) and RT using manual press (Carver, Wabash, IN, USA) to obtain the drug-loaded metal (Supplementary Materials ...
-
No products found
because this supplier's products are not listed.
D. Blaine Marchant, Brad Nelms, Virginia Walbot,
bioRxiv - Plant Biology 2022
Quote:
... 12.5 µL Ultra II Q5 Master Mix, 1 µL of 10 µM RP1, 1 µL of 10 µM RPI “X”). Libraries were amplified with 13 rounds of PCR (98□ for 30 sec ...
-
No products found
because this supplier's products are not listed.
Ju-Hyun An, et al.,
bioRxiv - Cell Biology 2019
Quote:
... cDNA samples were assayed using AMPIGENE® qPCR Green Mix Hi-ROX with SYBR Green Dye (Enzo Life Sciences, Farmingdale, NY, USA), according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Mariah Beaver, et al.,
bioRxiv - Genomics 2021
Quote:
... protein–DNA crosslinks were made at RT for 5 min with 1% formaldehyde and tissue was pulverized using the CryoPrep (Covaris Inc., MA, USA). Cells were lysed and nuclei were prepared using Covaris lysis buffer ...
-
No products found
because this supplier's products are not listed.
Leonardo Betancurt-Anzola, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The complex was incubated at RT for 20 mins and pipetted onto glowed discharged cryo-EM grids (Quantifoil R 2/2 Au 300). Grids were frozen in liquid ethane by using a Vitrobot Mark IV (ThermoFischer ...