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No products found
because this supplier's products are not listed.
Ayşe N. Erdoğan, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
... 8-oxo-2’-deoxyguanosine-5’-triphosphate (8-oxo-dGTP) and 2’-deoxy-P-nucleoside-5’-triphosphate (dPTP) (TriLink). For each library ...
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No products found
because this supplier's products are not listed.
John C. Obenauer, et al.,
bioRxiv - Neuroscience 2023
Quote:
The proteomics signature had 4 validation data sets: R6/2 mice aged 2 months (R6/2 2M) or 3 months (R6/2 3M) from PRIDE experiment PXD013771 ...
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No products found
because this supplier's products are not listed.
Dinesh Balasaheb Jadhav, Sougata Roy,
bioRxiv - Physiology 2023
Quote:
... About 4 μg of peptides were loaded on a trap column (ChromXP C18CL 5 µm 120 Å, Eksigent, SCIEX) and online desalting was performed with a flow rate of 10 µl per minute for 10 min ...
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No products found
because this supplier's products are not listed.
Arinze E. Okafor, et al.,
bioRxiv - Cell Biology 2022
Quote:
... and were blocked with Blocking buffer (4% BSC (Rockland, RLBSA50) and 1% goat serum (Gibco ...
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No products found
because this supplier's products are not listed.
Olivier Da Ines, et al.,
bioRxiv - Genetics 2022
Quote:
... 2 mM X-Gluc (5-bromo-4-chloro-3-indolyl-ß-D-glucuronic acid; Biosynth), dissolved in N,N-dimethylformamide) ...
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No products found
because this supplier's products are not listed.
Samuel Itskanov, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 3 μM Fos-Choline-8 (Anatrace) was added to the protein sample ...
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No products found
because this supplier's products are not listed.
Antonio Marino, et al.,
bioRxiv - Systems Biology 2023
Quote:
... 4 3 2.0 mm SecurityGuard (Phenomenex) cartridge as a guard column ...
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No products found
because this supplier's products are not listed.
Md Gulam Musawwir Khan, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Cell survival was measured using the WST-8 (water-soluble Tetrazolium-8: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)- 5- (2,4-disulfophenyl)-2H-tetrazolium) assay kit (CCK-8; Dojindo Molecular Technologies, #CK04) following manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Mohammed M. H. Albariqi, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Human calcitonin gene-related peptide α (CGRPα) and human calcitonin gene-related peptide 8–37 (CGRP 8-37) were obtained from Bachem (Switzerland) and dissolved in 0,9% NaCl (1 mg/ml ...
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No products found
because this supplier's products are not listed.
Julius Muller, et al.,
bioRxiv - Immunology 2019
Quote:
... (2 μg/ml/peptide, ANASPEC). Results are reported as spot-forming cells (SFC ...
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No products found
because this supplier's products are not listed.
Alexia Caillier, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The PVDF membranes were incubated overnight at 4◦C in 2% BSA (Research Products International, 9048-46-8) with primary antibody ...
-
No products found
because this supplier's products are not listed.
Ruofan Li, et al.,
bioRxiv - Immunology 2022
Quote:
... the wells were washed with “washing buffer” (PBS x1 and 0.05% Tween20) and were blocked for 2 h at RT with 200 µL of “blocking buffer” (PBS x1, 3% BSA (MP Biomedicals), 20 mM EDTA ...
-
No products found
because this supplier's products are not listed.
Mai Takenaka, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... riluzole (supply name: 2-amino-6-(trifluoromethyl)benzothiazole) and 4-chloro-3-ethylphenol (4-CEP) from Tokyo Chemical Industry (Tokyo, Japan), and 4-chloro-m-cresol (4-CMC ...
-
No products found
because this supplier's products are not listed.
Yoko Hayashi-Takanaka, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and centrifuged at 40,000 rpm (∼128,000 × g) for 3 h at 4°C using an MLS-5 rotor (Beckman Coulter). Aldolase (158 kDa ...
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No products found
because this supplier's products are not listed.
Aleksandra Boikova, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Cardiomyocyte specification was completed by 3 days of culture in RPMI B27-media with 5 μM IWP-4 (STEMCELL Technologies), followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher) ...
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No products found
because this supplier's products are not listed.
Tirumalasetty Muni Chandra Babu, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... MTT (3-[4, 5- dimethyl thiazol-2-yl]-2,5 diphenyl tetrazolium bromide) (Alfa Aesar Chemicals Co. Ltd. Shanghai, China), fetal bovine serum (FBS ...
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No products found
because this supplier's products are not listed.
Rotem Ben-Tov Perry, et al.,
bioRxiv - Molecular Biology 2022
Quote:
8 weeks-old C57BL/6J male mice (Envigo, Israel, n =3 per group) received bilateral stereotaxic injections of AAV9 Silc1 ...
-
The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell...
Cat# B34304, SKU# B34304-25 mL (2500 rxns),
25 mL (2500 rxns), $201.00
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Kristina A.M. Arendt, et al.,
bioRxiv - Cancer Biology 2020
Quote:
In vitro cell proliferation was determined using WST-8 [water soluble tetrazolium-8 or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphophenyl)-2H-teterazolium] assay (Bimake; Munich, Germany). For this ...
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No products found
because this supplier's products are not listed.
Lubna Amer, Maurice Retout, Jesse V. Jokerst,
bioRxiv - Bioengineering 2023
Quote:
The percent toxicity was calculated using an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) colorimetric assay (Biotium) wherein viable cells form an orange formazan product upon reduction ...
-
No products found
because this supplier's products are not listed.
Anna L. Vlasits, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Boroscillate pipettes (Sutter Instruments, 3-5 MΩ) were used for all recordings and whole-cell electrophysiology recordings were performed using a Multiclamp 700B amplifier (Molecular devices) ...
-
No products found
because this supplier's products are not listed.
Steffi Daniel, John D. Hulleman,
bioRxiv - Neuroscience 2022
Quote:
... fibulin-3 blocking peptide (5:1 to anti-fibulin-3 antibody, Prosci # 5213P). The next day ...
-
No products found
because this supplier's products are not listed.
Els F Halff, et al.,
bioRxiv - Neuroscience 2020
Quote:
... followed by overnight incubation at 4°C with primary antibody diluted in blocking solution (1:1000 Rabbit-α-SV2A, Abcam ab32942, Cambridge, UK; 1:300 Mouse-α-Neuroligin1/2/3/4, Synaptic Systems 129211, Göttingen, Germany). Sections were then washed in PBS (3×10 min ...
-
No products found
because this supplier's products are not listed.
Odile Fabre, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 2 mM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR, Toronto Research Chemicals), or 500 ng/mL mouse recombinant Gremlin 1 (R&D Biosciences ...
-
No products found
because this supplier's products are not listed.
Zhaoduan Liang, et al.,
bioRxiv - Immunology 2019
Quote:
... 5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium (BCIP/NBT, Mabtech) was used to develop the immune-spot according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Drew T. Dunham, et al.,
bioRxiv - Microbiology 2022
Quote:
... 5 μg of RNA was ran on a 8% polyacrylamide urea gel (National Diagnostics SequaGel 1:4) in 1x TBE buffer (100mM Tris base ...
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No products found
because this supplier's products are not listed.
Yun Liu, Weichun Lin,
bioRxiv - Neuroscience 2022
Quote:
... μ-conotoxin GIIIB (2 μM; Peptides International) was added to the bath solution 30 minutes prior to recording ...
-
No products found
because this supplier's products are not listed.
Yu-Chia Chen, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Samples for Immunostaining were fixed in 2% PFA or 4% 1-ethyl-3 (3-dimethylaminopropyl)-carbodiimide (EDAC, Carbosynth, Berkshire, UK). The fixed brains were dissected to enhance antigen presentation and to improve image quality.
-
No products found
because this supplier's products are not listed.
Pranav Vyas, et al.,
bioRxiv - Biophysics 2024
Quote:
... They were spawned during the spawning season (April to July) by injecting 5 ml of 2 nM NGLWYamide spawning peptide [82] (synthesized by Biomatik) at the anterior and posterior ends of the gonad ...
-
No products found
because this supplier's products are not listed.
Luke A Johnson, et al.,
bioRxiv - Neuroscience 2020
Quote:
... In all patients a directional “1-3-3-1” electrode was used (4/5 patients: Abbott Infinity model 6172 ...
-
No products found
because this supplier's products are not listed.
J. Christopher Rounds, et al.,
bioRxiv - Neuroscience 2021
Quote:
... sonicated for 3×5 minutes in a 4°C Bioruptor ultrasonicator (UCD-200, Diagenode), vortexed ...
-
No products found
because this supplier's products are not listed.
Ligia B. Schmitd, et al.,
bioRxiv - Neuroscience 2024
Quote:
... mice were anesthetized with isoflurane (5% induction, 2-3% maintenance, SomnoSuite Kent Scientific) 10d after the first SNC and the crush placed immediately proximal to the first one ...
-
No products found
because this supplier's products are not listed.
Zeynep Cakir, et al.,
bioRxiv - Neuroscience 2022
Quote:
Peptides were resuspended in 4% formic acid and 3% acetonitrile and directly injected on a 75 μm ID column (New Objective) packed with 25 cm of Reprosil C18 1.9 μm ...
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No products found
because this supplier's products are not listed.
Guojia Xie, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... and incubated with 4-8 μg primary target antibody and 2 μg spike-in antibody (Active Motif, #61686) overnight at 4°C ...
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Prepared to contain higher collagenase and caseinase activities. A dialyzed, lyophilized powder.
Cat# LS005282,
1 gm, $246.00
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Luke R. Perreault, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Minced tissue underwent 3-5 serial digestions in collagenase type 2 (Worthington Biochemical Corp, Lakewood, NJ) in sterile PBS with 20 mM glucose ...
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Cat# HY-N2038-5 mg,
5 mg, USD $343.0
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Shuangshuo Jia, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and the autophagy inhibitor 3-methyladenine (3-MA; 5 mM; MedChemExpress) were applied to validate their respective effects.
-
No products found
because this supplier's products are not listed.
Shuqin Ma, et al.,
bioRxiv - Microbiology 2022
Quote:
... After blocking with blocking solution (Solarbio), the membrane was incubated with GADPH (1:2000 ...
-
No products found
because this supplier's products are not listed.
Julianna L. Sun, et al.,
bioRxiv - Microbiology 2022
Quote:
... Membranes were developed using 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) (Sigma Aldrich, 11697471001. Images were captured using a Nikon Z1000 microscope.
-
No products found
because this supplier's products are not listed.
Brajabandhu Pradhan, et al.,
bioRxiv - Microbiology 2020
Quote:
... and bead beating 4 times for 2 minutes in a Mini-BeadBeater-8 (BioSpec) with cooling on ice in between.
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No products found
because this supplier's products are not listed.
Rene Yu-Hong Cheng, et al.,
bioRxiv - Bioengineering 2022
Quote:
... 5 µL of blocking antibody (anti-IgG, Southern Biotech) was added to the 50 µL cell solution to yield a final concentration of 25 µg/mL ...
-
No products found
because this supplier's products are not listed.
Felix Alonso-Valenteen, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Ligand blocking was performed by pre-adsorbing HPK with soluble human HER3 peptide (Sino Biological) at 10× molar excess in PBS before adding to cells ...
-
No products found
because this supplier's products are not listed.
Vikram Jakkamsetti, et al.,
bioRxiv - Neuroscience 2023
Quote:
We used a standard rotarod (rod diameter 3 cm, fall height 20 cm, start speed 4 rpm, acceleration rate 1 rpm/ 8 s, Touchscreen RotaRod, Harvard Apparatus) in several consecutive testing trials performed over 7 days while blinded to genotype and simultaneously testing control littermate mice ...
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No products found
because this supplier's products are not listed.
Yan Chen, et al.,
bioRxiv - Molecular Biology 2022
Quote:
The purified complex samples (3 μL at 5-8 mg/mL) were applied to glow-discharged holey grids (Quantifoil R1.2/1.3, 300 mesh) and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher ...
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No products found
because this supplier's products are not listed.
Matthew E. Griffin, et al.,
bioRxiv - Cell Biology 2023
Quote:
... before probing overnight at 4 °C with the following antibodies diluted in blocking buffer at 1:1000 unless otherwise specified: anti-OGT (Proteintech, Rosemont, IL; 11576-2-AP), anti-HA (C29F4) ...
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No products found
because this supplier's products are not listed.
Hema Priya Mahendran, et al.,
bioRxiv - Cancer Biology 2024
Quote:
Blocking Solution: 3% Albumin Fraction V (Bio Basic; CA#9048-46-8), 0.1% Tween20 (ACP Chemicals ...
-
No products found
because this supplier's products are not listed.
J Muir, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 3-(2-carboxypiperazin-4-yl) propyl-1phosphonic acid (CPP, 10 mM; HelloBio). Inhibitory postsynaptic currents (IPSCs ...
-
No products found
because this supplier's products are not listed.
Marco Schade, et al.,
bioRxiv - Paleontology 2022
Quote:
... 2 & 3 (Figs. 1–3; Supplementary Figs. 1–4) was performed using a Metrotom 1500 (Carl Zeiss Microscopy GmbH, Jena, Germany) in a subsidiary of Zeiss in Essingen ...
-
No products found
because this supplier's products are not listed.
Mariano Nicolás Di Guilmi, et al.,
bioRxiv - Neuroscience 2021
Quote:
... with a high-pass Bessel (8-pole) filter with a −3 dB cutoff of 5 Hz using Clampfit 10.6 (Molecular Devices). All data was analyzed using NeuroMatic in Igor Pro software (WaveMetrics ...
-
No products found
because this supplier's products are not listed.
Agata Szuba, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
-
No products found
because this supplier's products are not listed.
Jeroen van den Berg, et al.,
bioRxiv - Cell Biology 2019
Quote:
... a Lionheart FX automated microscope in combination with sirDNA77 staining was used to generate growth curves with a time resolution of 4 hours for a total time span of 136 hours (microscope maintained at 37°C, 5% CO2 using a 4× lens and a Sony CCD, 1.25 megapixel camera with 2 times binning; BioTek). Quantification of cell number was performed by Gen5 software (BioTek).
-
No products found
because this supplier's products are not listed.
Walden Li, Ryan M. Wyllie, Paul A. Jensen,
bioRxiv - Microbiology 2020
Quote:
... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...