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No products found
because this supplier's products are not listed.
Stefan Niekamp, Nico Stuurman, Ronald D. Vale,
bioRxiv - Biophysics 2019
Quote:
... 0.01 U pyruvate kinase (Sigma), 0.03 U lactate dehydrogenase (Sigma)) ...
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No products found
because this supplier's products are not listed.
Pengxiang Fan, et al.,
bioRxiv - Plant Biology 2020
Quote:
... 10 units pyruvate kinase (Roche, 10128155001), 10 units lactate dehydrogenase (Roche ...
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No products found
because this supplier's products are not listed.
Byung-Jun Sung, et al.,
bioRxiv - Physiology 2021
Quote:
... and pyruvate kinase (1:100, Cat #: ab38237, Abcam) overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Marco Di Gioia, et al.,
bioRxiv - Immunology 2023
Quote:
The inhibition of kinase activity mediated by phospholipids was determined using the following Z’-LYTE Kinase Assay Kits: Z’-LYTE Kinase Assay Kit - Ser/Thr 6 Peptide (Thermo Fisher Scientific, Cat# PV3179) used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Simone Cristina da Silva Rosa, et al.,
bioRxiv - Cell Biology 2020
Quote:
... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Lauren G. Buss, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... anti-muscle pyruvate kinase isoform 1 (PKM1) (Product no. D30G6, Cell Signaling), anti-complex I – 75 kDa subunit (ABN302 ...
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No products found
because this supplier's products are not listed.
Malgorzata Rychlowska, et al.,
bioRxiv - Microbiology 2022
Quote:
... Kinase activity was measured using ADP-Glo Kinase assay (V6930, Promega) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Samuel Rivero-Hinojosa, et al.,
bioRxiv - Cancer Biology 2021
Quote:
HLA-restriction of antigen recognition was tested in anti-IFN-γ ELISpot using autologous DCs loaded with the relevant peptide or without peptide (negative control) and blocking antibodies against HLA class I and II (both Dako). DCs were incubated with peptides overnight and the next day anti-HLA mABs were added to ELISpot plates for 1 hour prior to the addition of expanded cells at a ratio of 1 DC to 50 expanded cells ...
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No products found
because this supplier's products are not listed.
Christian Schmerling, et al.,
bioRxiv - Microbiology 2024
Quote:
... The glycerol concentration in the medium was determined enzymatically using the recombinantly produced Saci_2033 glycerol kinase (see below) and ADP formation was quantitatively assayed spectrophotometrically as oxidation of NADH via pyruvate kinase (PK) (Merck, Germany) and lactate dehydrogenase (LDH ...
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No products found
because this supplier's products are not listed.
Lan Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 50 U/mL of pyruvate kinase and lactate dehydrogenase was added to the wells of a 384-well black plate (Corning). 10 μM ATAD1 ...
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No products found
because this supplier's products are not listed.
Pierre-Gregoire Coulon, et al.,
bioRxiv - Immunology 2022
Quote:
... Sodium Pyruvate (Lonza), L-Glutamine ...
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No products found
because this supplier's products are not listed.
Wai Kit Ma, et al.,
bioRxiv - Cancer Biology 2021
Quote:
Pyruvate kinase activity was measured with a Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit (BioVision) in accordance with the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Tomer M. Yaron, et al.,
bioRxiv - Microbiology 2020
Quote:
Reagents used for the peptide library experiments include: Kinase substrate library (Anaspec). Streptavidin conjugated membranes (Promega) ...
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No products found
because this supplier's products are not listed.
VL Wyckelsma, et al.,
bioRxiv - Genetics 2020
Quote:
... After blocking in blocking buffer (LI-COR) and TBS-T for 1 h ...
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No products found
because this supplier's products are not listed.
Luciana Cañonero, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... and anti-Pyk1 (Polyclonal anti-pyruvate kinase (rabbit muscle) from Rockland) antibodies ...
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No products found
because this supplier's products are not listed.
Koya Yoshizawa, et al.,
bioRxiv - Cell Biology 2022
Quote:
... cells were co-treated with 10 µM MG132 (Peptide Institute; for blocking anaphase onset) and 100 nM SiR-DNA (Cytoskeleton inc. ...
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No products found
because this supplier's products are not listed.
Caterina Prelli Bozzo, et al.,
bioRxiv - Microbiology 2020
Quote:
... Gut organoids were treated with increasing concentrations (15 and 30 µg/ml) of IFITMs derived peptides (mouse IFITM2 antibody blocking peptide Santa Cruz sc-373676 P) and blocking antibodies (α-ACE2 AK (AC18Z) ...
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No products found
because this supplier's products are not listed.
Daisuke Tone, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Then 5 µl of each diluted cell lysate was mixed with 15 µl of cell lysis buffer containing 0.33 mM ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.) in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Giovanni Giuseppe Giobbe, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Rho-kinase inhibitor (Tocris) was added to single cell dissociated organoids ...
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No products found
because this supplier's products are not listed.
Uday Tak, Peace Walth, Aaron T. Whiteley,
bioRxiv - Microbiology 2023
Quote:
... blocking with Odyssey blocking buffer (Bio-Rad) for 1 hour shaking at room temperature ...
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No products found
because this supplier's products are not listed.
Rodrigo Vazquez-Lombardi, et al.,
bioRxiv - Immunology 2020
Quote:
Peptides and peptide libraries were generated by custom peptide synthesis (Genscript), re-suspended at 10 mg ml−1 in DMSO and placed at −80°C for prolonged storage ...
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No products found
because this supplier's products are not listed.
Claudia Dominici, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Blocking followed with M.O.M blocking reagent (Vector Laboratories) for 1 h at RT ...
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No products found
because this supplier's products are not listed.
Shirui Chen, et al.,
bioRxiv - Plant Biology 2021
Quote:
... 0.03 U/µl creatin kinase (Calbiochem)] ...
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No products found
because this supplier's products are not listed.
Ilona Berestjuk, et al.,
bioRxiv - Cancer Biology 2019
Quote:
Phospho-kinase screening was performed using a phospho-kinase array (Proteome Profiler Human Phospho-Kinase Array #ARY003B; R&D Systems) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Shani Ben-Moshe, et al.,
bioRxiv - Systems Biology 2019
Quote:
... FcX blocking solution (BioLegend) was added in a dilution of 1:50.
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No products found
because this supplier's products are not listed.
Rodrigo Guabiraba, et al.,
bioRxiv - Immunology 2023
Quote:
... a potent inhibitor of the noncanonical IkB kinases TANK-binding kinase-1 (TBK1) and IκB kinase-ε (IKKε) (Invivogen) were diluted in DMSO ...
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No products found
because this supplier's products are not listed.
Snježana Kodba, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Citron Kinase (BD Biosciences #611376), Acetylated Tubulin (Merck # T7451 and Cell Signaling Technology # D20G3) ...
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No products found
because this supplier's products are not listed.
Benjamin J. Meckiff, et al.,
bioRxiv - Immunology 2020
Quote:
... Cells were stimulated by the addition of individual virus-specific peptide pools (1 µg/ml) for 6 h in the presence of a blocking CD40 antibody (1 µg/ml; Miltenyi Biotec). For subsequent MACS-based enrichment of CD154+ ...
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No products found
because this supplier's products are not listed.
Emma S Chambers, et al.,
bioRxiv - Immunology 2020
Quote:
... After blocking in ECL blocking agent (GE Healthcare), membranes were probed with primary antibodies overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Theresa Schmid, et al.,
bioRxiv - Immunology 2023
Quote:
Peptides were generated by custom peptide synthesis (Biomatik) or provided as peptide pools (JPT or Miltenyi BioTec ...
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No products found
because this supplier's products are not listed.
Takuma Uo, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... Enzyme kinase activity was measure in the presence of 45 µM peptide substrate SAMStide (HMRSAMSGLHLVKRR) (SignalChem) in a buffered solution containing 5 mM MOP (pH 7.5) ...
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No products found
because this supplier's products are not listed.
Eunkyoung Shin, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and T4 polynucleotide kinase (Takara). The extension reaction was performed with the AccuPower® DNA Sequencing Kit (Bioneer) ...
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No products found
because this supplier's products are not listed.
Kirsten L. Viola, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Aβ1-42 (American Peptide or Peptides International) or FAM-Aβ1-42 (Anaspec ...
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No products found
because this supplier's products are not listed.
Yijin Chen, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Fms-related tyrosine kinase 3 ligand (PeproTech), and 0.5% penicillin/streptomycin (Invitrogen ...
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No products found
because this supplier's products are not listed.
Sara M. Blazejewski, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Neurons were treated with Neurobasal media containing 5 μM of kinase inhibitor from the DiscoveryProbeTM Kinase Inhibitor Library (APExBIO). The following inhibitors were used ...
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Ethyl pyruvate (CTI-01) is a simple aliphatic ester of pyruvic acid and has been shown to have...
Cat# S6243, SKU# S6243-25ul,
25ul, $97.00
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Regina M. Powers, et al.,
bioRxiv - Neuroscience 2022
Quote:
... phosphoinositide-3 kinase (PI3Kinase) inhibitor LY294002 (Selleck Chemicals).
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No products found
because this supplier's products are not listed.
Susanne Hellmuth, Olaf Stemmann,
bioRxiv - Cell Biology 2024
Quote:
... antigenic peptides (Bachem) were coupled via terminal Cys to Maleimide activated KLH (ThermoFisher ...
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No products found
because this supplier's products are not listed.
Sujung Choi, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... -pyruvate dehydrogenase kinase 1 (PDK1, Novus Biologicals, cat # 100-2383), - transcription factor II B (TFIIB ...
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No products found
because this supplier's products are not listed.
Henriette U. Balinda, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 1mM sodium pyruvate (VWR), penicillin and streptomycin (Invitrogen) ...
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No products found
because this supplier's products are not listed.
Aaron D. Stevens, Timothy N.J. Bullock,
bioRxiv - Immunology 2020
Quote:
... LFA blocking antibody (M17/4; BioXCell) was administered i.p ...
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No products found
because this supplier's products are not listed.
Gaowei Wang, et al.,
bioRxiv - Systems Biology 2023
Quote:
... and 20 µL Protein Kinase K (Qiagen) for 30 min at RT followed by the steps as described in the protocol of Dneasy Blood & Tissue Kit (QIAGEN) ...
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No products found
because this supplier's products are not listed.
Kwadwo A. Opoku-Nsiah, et al.,
bioRxiv - Biochemistry 2021
Quote:
Peptides were synthesized by Fmoc solid phase peptide synthesis on a Syro II peptide synthesizer (Biotage) at ambient temperature and atmosphere on a 12.5 μM using either pre-loaded Wang resin or Rink amide resin (Sigma-Aldrich) ...
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No products found
because this supplier's products are not listed.
Eric Poliner, et al.,
bioRxiv - Molecular Biology 2019
Quote:
The casein kinase inhibitor PF-670462 (Cayman Chemicals) was resuspended in water at 2 mg ml−1 ...
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No products found
because this supplier's products are not listed.
Shinobu Miyazaki-Anzai, et al.,
bioRxiv - Physiology 2023
Quote:
FLAG-IKK2 kinase inactive K44M (IKK2DN, Addgene, #15466) was cloned into pLenti-CMV-Puro DEST vector (Addgene ...
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Cat# HY-P7803-250 μg,
250 μg, USD $200.0
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Yuhang Wang, et al.,
bioRxiv - Immunology 2023
Quote:
The LRRK2 kinase inhibitor LRRK2-IN-1 (MedChemExpress) was reconstituted in corn oil as described previously (41) ...
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No products found
because this supplier's products are not listed.
Jennifer A. Korchak, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... a Rho-associated kinase (ROCK) inhibitor (StemCell Technologies), to promote cell survival ...
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No products found
because this supplier's products are not listed.
Hasan Abbas, et al.,
bioRxiv - Pathology 2020
Quote:
... a mouse IgG blocking antibody (Jackson Immunoresearch) was used in the blocking buffer and an antigen retrieval step was performed by heating the slides in a pressure cooker in citrate buffer was also performed prior to incubation with the primary antibody ...
-
No products found
because this supplier's products are not listed.
Marthe Solleder, et al.,
bioRxiv - Immunology 2019
Quote:
... Peptides were dried using vacuum centrifugation (Eppendorf Concentrator Plus ...
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No products found
because this supplier's products are not listed.
Lucia F. Zacchi, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Peptide abundance was measured with PeakView v2.2 (SCIEX) using the following parameters ...
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No products found
because this supplier's products are not listed.
Jakob Berner, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 1 mM sodium pyruvate (Pan Biotech), 25 mM HEPES (Pan Biotech) ...