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No products found
because this supplier's products are not listed.
Takuma Uo, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... AMPK activity in total cell lysates was measured using CycLex AMPK Kinase Assay kit (MBL International Corporation, Cat# CY-1182) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Yanyan Zheng, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Rabbit anti-PKA (1:1000, bs-0520R, Bioss, China), Rabbit anti-CREB (1:1000 ...
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No products found
because this supplier's products are not listed.
Trisiani Affandi, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Cells were then harvested and assayed for caspase-3 activity with the Caspase-3 Cellular Activity Assay Kit PLUS (Biomol, Farmingdale, NY), which uses N-acetyl-DEVD-p-nitroaniline as a substrate ...
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No products found
because this supplier's products are not listed.
Brunno R. Levone, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Proteins were quantified using a validated BCA (Bicinchoninic Protein Assay Kit, EuroClone) protocol ...
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No products found
because this supplier's products are not listed.
Lulu Yu, et al.,
bioRxiv - Cancer Biology 2020
Quote:
The cell proliferation assay based on dehydrogenase activities was performed by Cell Counting Kit-8 (Dojindo Molecular Technologies, Rockville, MD) as recommended ...
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No products found
because this supplier's products are not listed.
Maximilian Peer, et al.,
bioRxiv - Biochemistry 2022
Quote:
... to set up dual droplets for each condition with drop volume of 0.2 and 0.3 µl (1:1 and 2:1, protein vs reservoir solution) on 96-well sitting drop crystallization plates (Molecular Dimensions). The crystal used for final data collection was grown from a manually set up droplet containing 2 µl of protein and 1 µl of reservoir solution ...
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No products found
because this supplier's products are not listed.
Marion Schuller, et al.,
bioRxiv - Biochemistry 2020
Quote:
... assay plates using an Echo 525 Liquid Handler (Labcyte). Binding assays were conducted in a final volume of 16 μl with 12.5 nM Nsp3 Mac1 domain ...
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No products found
because this supplier's products are not listed.
Irene Molina-Gonzalez, et al.,
bioRxiv - Neuroscience 2022
Quote:
... GFAP-thymidine kinase mice (Jackson Laboratories) were used in which thymidine kinase from the herpes simplex virus is driven by the GFAP promoter ...
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No products found
because this supplier's products are not listed.
Jesyin Lai, Stephen V. David,
bioRxiv - Neuroscience 2019
Quote:
... Extracellular neurophysiological activity was recorded using 1-2 tungsten microelectrodes (FHC) or a 64-channel electrode array (Masmanidis Lab ...
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No products found
because this supplier's products are not listed.
Raphael J. Eberle, et al.,
bioRxiv - Biochemistry 2021
Quote:
SARS-CoV-2 3CLpro activity assay was performed using a fluorogenic substrate DABCYL- KTSAVLQ↓SGFRKME-EDANS (Bachem, Switzerland) in a buffer containing 20 mM Tris pH 7.2 ...
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No products found
because this supplier's products are not listed.
Eric Yixiao Cao, et al.,
bioRxiv - Immunology 2022
Quote:
... Plates were washed and incubated with 50μL of anti-mouse IgA-HRP (1:1000) in assay buffer (SouthernBiotech). Plates were washed and developed using TMB substrate (BioLegend) ...
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No products found
because this supplier's products are not listed.
Neserin Ali, et al.,
bioRxiv - Molecular Biology 2020
Quote:
The micro bicinchoninic acid (BCA) protein assay kit and Nanosep® 30K Omega Centrifugal Devices were purchased from Pall Life Sciences (Ann Arbor MI ...
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No products found
because this supplier's products are not listed.
Jonathan T. Lloyd, et al.,
bioRxiv - Biochemistry 2020
Quote:
... 66 were reproduced in hanging drops (1 μL protein solution plus 1 μL mother liquor) using 24-well VDX plates (Hampton Research) containing 500 μL mother liquor in the reservoir ...
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No products found
because this supplier's products are not listed.
Bjoern Meyer, et al.,
bioRxiv - Microbiology 2021
Quote:
... The assay plates were imaged on an IX-83 automated inverted microscope (Olympus) using a 10x objective ...
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No products found
because this supplier's products are not listed.
Sara M. Blazejewski, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and mRFP + Nuak 1 and 2 kinases was performed via chemical transfection using TransIT-X2 (Mirus Bio, Madison, WI) 48 hours following plating ...
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No products found
because this supplier's products are not listed.
Michael Uckelmann, et al.,
bioRxiv - Biophysics 2024
Quote:
... Assays were done in 384-well plates with #1.5 glass bottoms (MatTek PBK384G-1.5-C). The wells were treated with 1 M NaOH for 1 hour at room temperature ...
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No products found
because this supplier's products are not listed.
Chandima Bulumulla, et al.,
bioRxiv - Neuroscience 2022
Quote:
... TH-GFP signal (after activity imaging) was amplified using a rabbit anti-GFP (1:2000; Chromotek PABG1) antibody ...
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No products found
because this supplier's products are not listed.
Patrick O. Byrne, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... the proteins were biotinylated using the BirA biotin-protein ligase reaction kit (Avidity, #BirA500). Biotinylation of the NiV protein probes was confirmed by biolayer interferometry by testing the ability of the biotinylated protein to bind to streptavidin sensors ...
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No products found
because this supplier's products are not listed.
Laine Lysyk, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Enzyme activity was quenched by adding GuHCl (MP Biomedicals) to a final concentration of 6.4 M and samples were immediately stored at −80 °C ...
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No products found
because this supplier's products are not listed.
Fang Lu, et al.,
bioRxiv - Microbiology 2022
Quote:
... The MeDIP or hMeDIP assays were performed using MagMeDIP kit (Diagenode, C02010021) or hMeDIP kit (Diagenode ...
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No products found
because this supplier's products are not listed.
Veronica Birdsall, et al.,
bioRxiv - Neuroscience 2021
Quote:
... sapphire disks were placed into 12-well plate containing 1 mL of 2% glutaraldehyde (EMS #16530) and 1 mM CaCl2 (Sigma-Aldrich # 63535 ...
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No products found
because this supplier's products are not listed.
Paresh P Kulkarni, et al.,
bioRxiv - Cell Biology 2023
Quote:
Protein C activity in WT and Apoh-/- plasma was performed using the Chromogenix Coamatic Protein C activity kit (Diapharma). Briefly ...
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No products found
because this supplier's products are not listed.
I.R. Akberdin, et al.,
bioRxiv - Systems Biology 2021
Quote:
... Another target of calmodulin is Ca2+/calmodulin-dependent protein kinase kinase 2 (CAMKK2) that phosphorylates AMPK Thr172 thereby activating the kinase (Abbott et al., 2009). In turn ...
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No products found
because this supplier's products are not listed.
Shruti Shankar, et al.,
bioRxiv - Neuroscience 2021
Quote:
... sgRNA activity was confirmed by in vitro cleavage assays with purified recombinant Cas9 protein (PNA Bio, Inc., CP01-200) following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Michèle Reindl, et al.,
bioRxiv - Microbiology 2020
Quote:
... Quantitative Gus and LacZ liquid assays were based on the chromogenic and fluorescent substrates ONPG for the liquid LacZ activity assay (o-nitrophenyl-β-D-galactopyranoside) and MUG for the liquid Gus activity assay (4-methylumbelliferyl-ß-D-glucuronide trihydrate; BioWorld, 30350000-2 (714331), respectively (Stock et al. ...
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No products found
because this supplier's products are not listed.
Laura Cheradame, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Cell lysate protein concentration was determined using a Micro BCA Protein Assay kit (Bio Basic). Unless specified ...
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No products found
because this supplier's products are not listed.
Caila A. Pilo, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Lipids used in kinase assays (DG, cat #800811C and PS, cat #840034C) were purchased from Avanti Polar Lipids. The anti-phospho-PKCγ hydrophobic motif (pThr674 ...
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No products found
because this supplier's products are not listed.
Elizabeth C. Stahl, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The plate-reader based LAL assay was performed with the Endosafe Endochrome-K kit (Charles River, #R1708K), where a control standard endotoxin (CSE ...
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No products found
because this supplier's products are not listed.
Yun Tian, et al.,
bioRxiv - Microbiology 2021
Quote:
... for PCR-based assays or a column-based RNA/DNA/protein purification kit (Norgen Biotek, Ontario, Canada) for transcriptomic analysis ...
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No products found
because this supplier's products are not listed.
Johanna L. Mehl, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Protein concentrations were determined with a BCA protein assay kit (23225, Thermo Fischer Scientific) and cell lysates were incubated with 5X Reducing Sample Buffer (0.3M Tris-HCL pH6.8 ...
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No products found
because this supplier's products are not listed.
Pavel A. Makhnovskii, et al.,
bioRxiv - Physiology 2023
Quote:
... with 1% beta-mercaptoethanol in a 1.5-ml tube using a polypropylene pestle and a drill and then incubated (55 °C, 10 min) with protein kinase K (Evrogen, Russia). Total RNA was extracted by a silica spin column (CleanRNA Standard ...
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No products found
because this supplier's products are not listed.
Yang Wang, et al.,
bioRxiv - Microbiology 2024
Quote:
... 96-well plates (JET BIOFIL) were coated with recombinant spike proteins of Wuhan-hu-1 (ACROBiosystems, SPN-C52H4), BA.2.75.2 (ACROBiosystems ...
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No products found
because this supplier's products are not listed.
Hugo Belda, et al.,
bioRxiv - Microbiology 2024
Quote:
... 3µl kinase at 40nM in kinase reaction buffer (20mM MOPS, 10mM magnesium chloride and 10mM manganese chloride, pH 7.4, Alfa Aesar) was dispensed in each well using a Multidrop™ Combi Reagent dispenser (ThermoFischer Scientific) ...
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No products found
because this supplier's products are not listed.
Jing Xia, et al.,
bioRxiv - Plant Biology 2022
Quote:
... 40 nM [3H]IAA (specific activity 25 Ci mmol−1, American Radiolabeled Chemicals) and 50 µM inhibitors ...
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No products found
because this supplier's products are not listed.
Xiangchen Li, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... Quant-iT PicoGreen dsDNA Assay Kit (ABI), First Strand cDNA Synthesis Kit (Servicebio) ...
-
No products found
because this supplier's products are not listed.
Souvik Ghosh, et al.,
bioRxiv - Cell Biology 2019
Quote:
Migration assay was performed with CIM plates using the xCELLigence system (RTCA, ACEA Biosciences). 3×104 cells were plated in each well according to manufacturer’s instruction and migration was monitored up to 48 hrs after seeding using the RTCA-integrated software ...
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No products found
because this supplier's products are not listed.
Devorah M. Natelson, et al.,
bioRxiv - Physiology 2019
Quote:
... HbA1c levels were assessed using an enzymatic assay kit (Mouse Hemoglobin A1c Assay Kit #80310, Crystal Chem, Elk Grove Village, IL, USA) according to manufacturer’s instructions.
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No products found
because this supplier's products are not listed.
Margaret A. Potts, et al.,
bioRxiv - Cancer Biology 2024
Quote:
The CUT and RUN assay was performed using CUTANA™ CUT and RUN Assays kit (Epicypher, v2.0) following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Anne Drougard, et al.,
bioRxiv - Physiology 2023
Quote:
... 30 and 60 with the Elisa insulin assay kit (Mercodia).
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No products found
because this supplier's products are not listed.
Qiuying Liu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Colonies were stained using an Alkaline Phosphatase Assay Kit (System Biosciences) and evaluated under an Olympus CK2 microscope.
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No products found
because this supplier's products are not listed.
M. Beukema, et al.,
bioRxiv - Immunology 2023
Quote:
... were determined by measuring the hemagglutination activity of virus suspensions that were serially diluted in the presence of 1% guinea pig erythrocytes obtained from Envigo. HAU were calculated as the reciprocal of the highest virus dilution causing complete hemagglutination of erythrocytes ...
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Chromatographically purified for higher specific activity. Same as Code: ZF except assayed...
Cat# LS004004,
10 ku, $515.00
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Eunjae Park, et al.,
bioRxiv - Bioengineering 2019
Quote:
... and suspended in collagenase solution (Worthington, CLSPA: Activity 1090 Units/mg) and kept at 37ºC until complete degradation ...
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No products found
because this supplier's products are not listed.
Dazhi Li, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The protein was extracted by incubating with 1% GDN (Anatrace) on a rotator for 2 hours at 4C ...
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No products found
because this supplier's products are not listed.
Federico Ceriani, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... IHC activity was imaged and acquired using NIS-Elements software (Nikon Instruments, Inc.) at 0.5 – 2 Hz frame rate and analyzed later offline ...
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No products found
because this supplier's products are not listed.
John D. Klement, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 96 flat-bottom plates were coated with 1 μg/mL 145-2C11 (BioXcell), IgG (MOPC-21 ...
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No products found
because this supplier's products are not listed.
Xinyu Xie, et al.,
bioRxiv - Immunology 2024
Quote:
Multiplexed immunohistochemistry (mIHC) assay was performed using the Opal 6-Plex Detection Kit (AKOYA #811001, USA) as described previously73 ...
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No products found
because this supplier's products are not listed.
Oluwadamilola O. Lawal, et al.,
bioRxiv - Neuroscience 2024
Quote:
... chicken anti-green fluorescent protein (1:1000 [GFP-1020, Aves Labs, CA]), Guinea pig anti-VGAT (Synaptic Systems #131004) ...
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No products found
because this supplier's products are not listed.
Chi Wang, et al.,
bioRxiv - Biophysics 2022
Quote:
A 3 µl aliquot of freshly concentrated or thawed protein at ∼1 mg/ml was deposited on an UltraAuFoil 300 mesh R 0.6/1 grid (Quantifoil Inc., UK) at 4 °C and 100% humidity in a Vitrobot Mark IV robot (Thermo Fisher) ...
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No products found
because this supplier's products are not listed.
Hana Nůsková, et al.,
bioRxiv - Molecular Biology 2022
Quote:
C15-az and C17-az modified proteins were pulled down using the Click Chemistry Capture Kit (Jena Bioscience) and alkyne agarose beads (Jena Bioscience) ...
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No products found
because this supplier's products are not listed.
Travis C. Glenn, et al.,
bioRxiv - Genomics 2019
Quote:
... Protocol 1: EZNA Tissue DNA KIT (Omega Bio-Tek, USA); Protocol 2 ...