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No products found
because this supplier's products are not listed.
Lucas J. D’Souza, et al.,
bioRxiv - Immunology 2021
Quote:
Genomic DNA was isolated from gRNA-transduced cultures at the time of assay using a DNA extraction kit (IBI Scientific). Primers were designed +/-150bp from the PAM site of the targeting gRNA and used to amplify 300bp amplicons from 1μg genomic DNA using the Q5® DNA polymerase (M0491 ...
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No products found
because this supplier's products are not listed.
Debangshu Samanta, et al.,
bioRxiv - Cancer Biology 2022
Quote:
The Aldefluor assay (StemCell Technologies) was utilized to identify ALDH+ cells ...
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No products found
because this supplier's products are not listed.
Hui Chen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The proteins were first purified using CaptivA Protein A affinity resin (Repligen), unless otherwise specified ...
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No products found
because this supplier's products are not listed.
Laurie Arnaud, et al.,
bioRxiv - Neuroscience 2021
Quote:
... as human recombinant proteins (Peprotech). APOE3 was resuspended in 20 mM Sodium Phosphate ...
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No products found
because this supplier's products are not listed.
David Gonzalez-Perez, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Proteins were separated by SDS-PAGE (12% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and transferred to PVDF membranes using an iBlot2 Gel Transfer Device (Thermo Fisher Scientific) ...
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No products found
because this supplier's products are not listed.
Huihui Mou, et al.,
bioRxiv - Microbiology 2020
Quote:
... and the interaction was detected with goat-anti-human-Ig-APC (Jackson ImmunoResearch Laboratories, Inc). Expression of ACE2 orthologs were detected using mouse monoclonal antibody against c-Myc antibody 9E10 (Thermo Fisher ...
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No products found
because this supplier's products are not listed.
Elvio Blini, et al.,
bioRxiv - Neuroscience 2020
Quote:
We anticipated three possible scenarios for the three-way interaction Congruency by Condition by GVS, graphically depicted in Figure 2C ...
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No products found
because this supplier's products are not listed.
Evelyn M. Mrozek, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... The protein concentration was determined using a Bradford assay (Boston BioProducts). For immunoblotting ...
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No products found
because this supplier's products are not listed.
Pan Zhang, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... The total protein levels of the lysates were measured by BCA protein assay kit (iNtRON Biotechnology, Cat No. 21071). The following primary antibodies were used at 1:1000 dilution ...
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No products found
because this supplier's products are not listed.
Anissa A. Widjaja, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and Relative Human Mitochondrial DNA copy number Length Quantification qPCR Assay Kit (8938, ScienCell), respectively ...
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No products found
because this supplier's products are not listed.
Mithu Baidya, et al.,
bioRxiv - Cell Biology 2019
Quote:
... after a 3 minutes pre-incubation with of 2.5 µM coelenterazine H (BRET1 for V2R β-arrestin interaction) from Goldbio or coelenterazine 400a (BRET2 for CAAX and FYVE assays ...
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No products found
because this supplier's products are not listed.
Sylvia Torres-Odio, et al.,
bioRxiv - Immunology 2020
Quote:
... The supernatant was collected as protein lysate and quantified with micro-BCA assay (23235, Proteintech). Between 20-30μg of protein were separated on 10-20% polyacrylamide gradient gels ...
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No products found
because this supplier's products are not listed.
Andrew E Hale, et al.,
bioRxiv - Microbiology 2020
Quote:
Double stranded DNA probes were incubated with 500ng purified recombinant FOXO3a protein (Abnova) on ice for 20 minutes ...
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No products found
because this supplier's products are not listed.
Valery F. Thompson, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Detection of eluted proteins was made by western blot assay using anti-FUS (A300-294A, Bethyl Laboratories) to identify FUS protein and anti-His (HIS.H8 ...
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No products found
because this supplier's products are not listed.
Duanfeng Xin, et al.,
bioRxiv - Plant Biology 2023
Quote:
... The protein quantification was performed via the Detergent Compatible Bradford Protein Assay Kit (P0006C, Beyotime, China) and Western blotting was performed according to the manufacturer’s protocol from ABclonal (https://abclonal.com/Uploads/protocol/WB_protocol.pdf) ...
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No products found
because this supplier's products are not listed.
Andrey Krivoy, et al.,
bioRxiv - Genomics 2022
Quote:
... The lack of detectable DNA in the DNase-treated samples was confirmed by qPCR assay (QuantumDNA-Set, Evrogen QS004). RNA concentrations were measured using Qubit™ RNA HS Assay Kit (Invitrogen Q32852).
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No products found
because this supplier's products are not listed.
Elizabeth A. Brija, et al.,
bioRxiv - Neuroscience 2023
Quote:
... In vitro kinase assays were performed using purified recombinant Cpx proteins and the catalytic subunit of CK2 (C70-10G, SignalChem). Briefly ...
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No products found
because this supplier's products are not listed.
Andrea Galisova, et al.,
bioRxiv - Bioengineering 2022
Quote:
... ACE2-expressing HEK293T and HEK293T control cells were lysed and the activity of the ACE protein was assessed by the SensoLyte 390 ACE2 Activity Assay Kit (AnaSpec, USA) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
John R. Ferdinand, et al.,
bioRxiv - Physiology 2019
Quote:
... Enzyme Linked Immunosorbent Assays (ELISA) were used to detect protein levels in the perfusate for CHIT1 (BioTeche Novus Biologicals, Abingdon, Oxfordshire, UK), SCGB1A1 and soluble Intercellular Adhesion Molecule 1 (ICAM-1 ...
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No products found
because this supplier's products are not listed.
Alina Tscherne, et al.,
bioRxiv - Microbiology 2021
Quote:
... ELISPOT assay (Mabtech ELISpot kit for mouse IFN-γ ...
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No products found
because this supplier's products are not listed.
Sonia Rodríguez-Fdez, et al.,
bioRxiv - Biochemistry 2020
Quote:
... The rest of the plasmids encoding Vav1 mutant proteins were generated in this work by site-directed mutagenesis using the high-fidelity NZYProof DNA polymerase (Cat. #14601, NZYTech) and the appropriate combination of mutation-bearing oligonucleotides (Table S1).
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No products found
because this supplier's products are not listed.
Lei Zhang, José M Jiménez-Gómez,
bioRxiv - Plant Biology 2019
Quote:
... Total protein was extracted using PEB protein extraction buffer (Agrisera Antibodies) according to the manufacturer‘s instructions ...
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No products found
because this supplier's products are not listed.
Margaret A. Potts, et al.,
bioRxiv - Cancer Biology 2024
Quote:
The CUT and RUN assay was performed using CUTANA™ CUT and RUN Assays kit (Epicypher, v2.0) following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Yisong Qian, et al.,
bioRxiv - Immunology 2021
Quote:
... S protein (SPN-C52H4) and envelope protein (ENN-C5128) were from Acrobiosystems (Newark, DE). SARS-CoV-2 NSP1 (97-095) ...
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No products found
because this supplier's products are not listed.
Jürgen G. Okun, et al.,
bioRxiv - Physiology 2020
Quote:
... or protein-enriched (80%E protein, 10% carbohydrate, 10% fat; D16080303, Research Diets, USA) diets for a period of 3 weeks with various metabolic phenotyping tests ...
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No products found
because this supplier's products are not listed.
Jingqi Zhou, et al.,
bioRxiv - Genomics 2021
Quote:
Zebrafish’s bone morphogenetic protein (BMP) and bone morphogenetic protein receptor genes were used as query to search the BMP and BMP receptor gene families in P ...
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No products found
because this supplier's products are not listed.
Xenia Dolde, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
The µ-slide chemotaxis assay (Ibidi, Martinsried, Germany) allows the establishment of a stable gradient and the observation of cells within this gradient via time-lapse imaging ...
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No products found
because this supplier's products are not listed.
Aditya Singh, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and b) relative partner-interaction time (RPI), defined as the ratio of time spent interacting with (pointed towards ...
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No products found
because this supplier's products are not listed.
Juan Enriquez-Traba, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Social interaction was automatically scored with AnyMaze software (Stoelting, Kiel, WI) and defined as the ratio of time spent in the interaction zone with a juvenile mouse (i.e ...
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No products found
because this supplier's products are not listed.
Joseph H.A. Vissers, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... The Tgi mutant constructs used for interaction domain mapping were synthesized (Biomatik) and subcloned into pMK33-SBP-C using SpeI and XhoI ...
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No products found
because this supplier's products are not listed.
Nirdosh Dadwal, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The dynamic T cell interaction was recorded under physiologic shear stress (1.5 dyn/cm2) with a monochrome CCD camera (AxioCam MRm Rev, Carl Zeiss) using a 20x objective (LD Plan Neofluar) ...
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No products found
because this supplier's products are not listed.
Shayna M. Mueller, et al.,
bioRxiv - Neuroscience 2023
Quote:
To study astrocyte/endothelial interactions the mouse brain endothelioma cell line bEND.3 was used (American Type Culture Collection CRL-2299, RRID:CVCL_0170) due to the advantages of a homogeneous population of cells and to reduce the number of animals used ...
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No products found
because this supplier's products are not listed.
Johanna L. Mehl, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Protein concentrations were determined with a BCA protein assay kit (23225, Thermo Fischer Scientific) and cell lysates were incubated with 5X Reducing Sample Buffer (0.3M Tris-HCL pH6.8 ...
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No products found
because this supplier's products are not listed.
Changyang Linghu, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
The DNAs encoding the protein motifs used in this study were mammalian-codon optimized and synthesized by Epoch Life Science and then cloned into mammalian expression backbones ...
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No products found
because this supplier's products are not listed.
Avirup Malla, Suvroma Gupta, Runa Sur,
bioRxiv - Cancer Biology 2023
Quote:
... pyruvate assay kit (Elabscience), lactate assay kit (Elabscience) ...
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No products found
because this supplier's products are not listed.
Leying Chen, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... or NEFA assay (Wako Diagnostics) was used to measure lipolysis ...
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No products found
because this supplier's products are not listed.
Ikumi Miyazaki, et al.,
bioRxiv - Biochemistry 2024
Quote:
A commercial l-lactate assay kit (Lactate Assay Kit-WST, L256, Dojindo Molecular Technologies, Inc.) was used to quantify the l-lactate uncaged from MNI-l-lac upon light illumination ...
-
No products found
because this supplier's products are not listed.
Flavia Scoyni, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... the luciferase assay was conducted with the Luc-Pair Luciferase Assay Kit 2.0 (LF001-GC, GeneCopoeia), following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Akihiko Yamamoto, et al.,
bioRxiv - Genetics 2024
Quote:
... Fragmented DNA was used to produce barcoded DNA libraries using NEXTflex™ DNA Barcodes (Bioo Scientific, Inc.) with either the TruSeq® DNA Library Prep Kit (Illumina ...
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No products found
because this supplier's products are not listed.
Matthäus Mittasch, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Protein-Free (Expression Systems), supplemented with Fetal Bovine Serum (2% final concentration).
-
No products found
because this supplier's products are not listed.
Yfat Yahalom-Ronen, et al.,
bioRxiv - Microbiology 2020
Quote:
... L and G proteins (Kerafast), all of which under T7 promoter control ...
-
No products found
because this supplier's products are not listed.
Derek Aspacio, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
The Amplite Colorimetric NADH Assay Kit (AAT Bioquest) with some components from the redox ratio kits were reformulated to prepare an NMNH/NMN+ kit ...
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No products found
because this supplier's products are not listed.
Chih-Chieh Wang, et al.,
bioRxiv - Neuroscience 2021
Quote:
... DNA sequencing (Eton Biosciences) and protein mass spectrometry analysis (Harvard Mass Spectrometry and Proteomics Resource Laboratory ...
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No products found
because this supplier's products are not listed.
Christine Olsen, et al.,
bioRxiv - Bioengineering 2022
Quote:
... All solutions containing proteins were prepared in protein low binding tubes from Sarstedt (Nümbrecht, Germany). The 1 mg/mL stock solutions were further diluted to working solutions consisting of 10 ng/μL human or bovine insulin ...
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No products found
because this supplier's products are not listed.
Jasper Che-Yung Chien, et al.,
bioRxiv - Genetics 2020
Quote:
Genomic DNA was collected using a Genomic DNA Extraction Kit (Favorgen, Pingtung, Taiwan). For gRNA test samples ...
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WB,ELISA
Cat# A5473, SKU# A5473-100ul,
100ul, $157.00
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Xuxiao He, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... The recombinant proteins were purified using GSH Magnetic Beads for GST tag protein purification (Bimake, B23702) and Nickel Magnetic Beads for His tag protein purification (Bimake ...
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No products found
because this supplier's products are not listed.
Bongyong Lee, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... For growth inhibition assays with AX15836 (Tocris, Minneapolis, MN) in 2D culture systems ...
-
No products found
because this supplier's products are not listed.
Felix Grünberger, et al.,
bioRxiv - Microbiology 2020
Quote:
... A DNA sequencing ladder was generated using a DNA cycle Sequencing Kit (Jena Bioscience) according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Vivien Horváth, et al.,
bioRxiv - Neuroscience 2023
Quote:
DNA was extracted from frozen pellets using the Nanobind HMW DNA Extraction kit (PacBio) following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Seyedehmahsa Moghimi, et al.,
bioRxiv - Microbiology 2022
Quote:
... using Mirus 2020 DNA transfection reagent (Mirus). The infectious virions were harvested in the culture supernatant 48 h post-transfection ...