-
No products found
because this supplier's products are not listed.
Peter Verstraelen, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Fluorescent labeling of intermediate curli was performed using a HiLyte Fluor 555 protein labeling kit (Anaspec AS-72045). An aliquot of curli or curli-HiLyte555 was thawed from −80 °C ...
-
No products found
because this supplier's products are not listed.
Ine Vanderleyden, et al.,
bioRxiv - Immunology 2019
Quote:
NP-KLH (Conjugation ratio 29-33, Biosearch Technologies) was dissolved in PBS to 1 mg/ml and mixed with Imject Alum (ThermoFisher Scientific ...
-
No products found
because this supplier's products are not listed.
Hisako Kaneda, et al.,
bioRxiv - Biochemistry 2020
Quote:
... which was produced by the conjugation reaction between anti-fluorescein antibody (600101096; Rockland Immunochemicals ...
-
No products found
because this supplier's products are not listed.
Erez Dror, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and chromatin labeling using conjugated H3K27me3 (Origene, TA347154 conjugated using mix-n-stain CF555 kit ...
-
No products found
because this supplier's products are not listed.
Yidi Sun, et al.,
bioRxiv - Cell Biology 2019
Quote:
GFP nanobody Alexa Fluorphore 647 conjugation: A 100 µl GFP nanobody solution at 71µM (gt-250, ChromoTek) was conjugated to the Alexa Flurophore™ 647 NHS ester (A20006 ...
-
No products found
because this supplier's products are not listed.
Ayano Kondo, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... successful conjugation and purification was verified by denaturing gradient gel electrophoresis (BioRad, TGX Mini-Protean 4-15%) and Coomassie staining (Thermo Fisher) ...
-
No products found
because this supplier's products are not listed.
Viktória Szentgyörgyi, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Hungary)-containing Mowiol 4.88 (for nuclei labeling, Polysciences, Germany).
-
No products found
because this supplier's products are not listed.
Ziyun Shen, et al.,
bioRxiv - Biochemistry 2023
Quote:
... full-wavelength multifunctional enzyme labeling instrument (BMG Labtech, Germany), oscillator (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Vishnu Muraleedharan Saraswathy, et al.,
bioRxiv - Neuroscience 2021
Quote:
For simultaneous labeling with rabbit anti-Sox2 (GeneTex, 124477, 1:250) and rabbit anti- pSmad3(S423/425 ...
-
No products found
because this supplier's products are not listed.
Rohan Dandage, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... for “medium” (M) labeling and (3) 13C6 15N2 L-Lysine (Cambridge Isotope Laboratories) for “heavy” (H ...
-
No products found
because this supplier's products are not listed.
May Daher, et al.,
bioRxiv - Immunology 2020
Quote:
... Cas9 protein (PNA bio) and gRNA were incubated at room temperature for 15 min in a 3:1 reaction with 15μg Cas9 and 5000 ng gRNA (2500ng of sgRNA#1 and 2500ng of sgRNA#2).47 The incubation product (gRNA and Cas9 complex ...
-
No products found
because this supplier's products are not listed.
Xiangchen Li, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... enzyme labeling instrument (BioTek, FLX800T ...
-
No products found
because this supplier's products are not listed.
Erez Dror, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The presented data in Figure S1F shows additional labeling with H3K27me3 (C15410195, Diagenode). Unless stated differently-insulin positive β-cells were analyzed and sorted while excluding glucagon ...
-
No products found
because this supplier's products are not listed.
Michał A. Surma, et al.,
bioRxiv - Biochemistry 2021
Quote:
... healthy diets differing by protein content: Teklad global 14% protein (Envigo #2014S, low protein) and Teklad global 18% protein (Envigo #2018S ...
-
No products found
because this supplier's products are not listed.
Muaz Nik Rushdi, et al.,
bioRxiv - Immunology 2022
Quote:
... The purified proteins were biotinylated using biotin protein ligase (Avidity); excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare) ...
-
No products found
because this supplier's products are not listed.
Stefanie Krug, et al.,
bioRxiv - Immunology 2021
Quote:
... Protein concentrations were determined by CB-X protein assay (G-Biosciences) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Qiuling Wang, Suiwen Hou,
bioRxiv - Plant Biology 2022
Quote:
... Protein concentration was determined using BCA protein assay kit (Solarbio, PC0021).
-
No products found
because this supplier's products are not listed.
Liangqi Xie, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... The conjugation reaction was further precipitated by 3M sodium acetate ...
-
No products found
because this supplier's products are not listed.
Jonathan Perr, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Cell surface protein labeling was accomplished using sulfo-NHS-biotin (APExBio, no disulfide bond) as described above after which crude membrane fractions were isolated as described for the adherent cells ...
-
No products found
because this supplier's products are not listed.
Yana van der Weegen, et al.,
bioRxiv - Molecular Biology 2019
Quote:
Purified xlCRL4CSA was neddylated in vitro using the NEDD8 Conjugation Initiation Kit (Boston Biochem) according to the manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Teemu P. Miettinen, et al.,
bioRxiv - Cell Biology 2021
Quote:
... the last 20 min of antibody labeling was carried out together with DNA labeling using NUCLEAR-ID red DNA stain at 1:250 dilution (Enzo Life Sciences, Cat#ENZ-52406). For microscopy ...
-
No products found
because this supplier's products are not listed.
Fakhriedzwan Idris, et al.,
bioRxiv - Microbiology 2024
Quote:
... made by conjugating HRP to Mab56.2 using NH2 peroxidase labeling kit (Abnova) and incubated for 1.5 h at room temperature ...
-
No products found
because this supplier's products are not listed.
Alena Rudkouskaya, et al.,
bioRxiv - Bioengineering 2019
Quote:
... The degree of labeling of the probes was assessed by spectrophotometer DU 640 (Beckman Coulter, Fullerton, CA, USA). The average degree of labeling was no more than 2 fluorophores per Tf molecule ...
-
No products found
because this supplier's products are not listed.
Allison J. Greaney, et al.,
bioRxiv - Microbiology 2021
Quote:
... Secondary labeling was performed with goat anti-human IgG-Fc horseradish peroxidase (HRP) (1:3000, Bethyl Labs, A80-104P). Antibody binding was detected with TMB/E HRP substrate (Millipore Sigma ...
-
No products found
because this supplier's products are not listed.
Daniel Bormann, et al.,
bioRxiv - Neuroscience 2023
Quote:
... For some staining’s we directly labelled primary antibodies using FlexAble CoraLite® Plus Antibody Labeling Kits (Proteintech, Rosemont, IL, USA), as per manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Tom Baladi, et al.,
bioRxiv - Biochemistry 2020
Quote:
... products stemming from the TdT-labeling of oligonucleotide TdT3 were irradiated with UV light prior and after purification with a NucleoSpin (Macherey-Nagel) clean-up kit.
-
No products found
because this supplier's products are not listed.
Akanksha Agrawal, et al.,
bioRxiv - Cell Biology 2022
Quote:
... were assessed by terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) assay with an in situ Cell Death Detection Kit (Biovision Inc., CA, USA) as per the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Lei Zhang, José M Jiménez-Gómez,
bioRxiv - Plant Biology 2019
Quote:
... Total protein was extracted using PEB protein extraction buffer (Agrisera Antibodies) according to the manufacturer‘s instructions ...
-
No products found
because this supplier's products are not listed.
Pablo Castro-Córdova, et al.,
bioRxiv - Microbiology 2020
Quote:
... and protein concentration was quantified by BCA protein kit (RayBiotech USA). Next ...
-
No products found
because this supplier's products are not listed.
Hui Chen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The proteins were first purified using CaptivA Protein A affinity resin (Repligen), unless otherwise specified ...
-
No products found
because this supplier's products are not listed.
Stefan Kol, et al.,
bioRxiv - Bioengineering 2019
Quote:
... protein A biosensors (Pall) were equilibrated in sample diluent buffer (PBS containing 0.1% BSA and 0.02% Tween-20) ...
-
No products found
because this supplier's products are not listed.
Yisong Qian, et al.,
bioRxiv - Immunology 2021
Quote:
... S protein (SPN-C52H4) and envelope protein (ENN-C5128) were from Acrobiosystems (Newark, DE). SARS-CoV-2 NSP1 (97-095) ...
-
No products found
because this supplier's products are not listed.
Laurie Arnaud, et al.,
bioRxiv - Neuroscience 2021
Quote:
... as human recombinant proteins (Peprotech). APOE3 was resuspended in 20 mM Sodium Phosphate ...
-
No products found
because this supplier's products are not listed.
Krystal C. Johnson, et al.,
bioRxiv - Biochemistry 2023
Quote:
... recombinant AGO2 protein (Active Motif) was purchased and serially diluted in Protein LoBind tubes (Eppendorf ...
-
No products found
because this supplier's products are not listed.
Fang Xu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... The position of the scanner was in conjugation with the back focal plane of an illumination objective (10X/NA 0.3, Olympus) via two coupled relay lenses (F=150mm ...
-
No products found
because this supplier's products are not listed.
Anoma Nellore, et al.,
bioRxiv - Immunology 2021
Quote:
... IgG capture beads were generated by the direct conjugation of goat anti-Human IgG F(ab)2 (Southern Biotech) to array particles (Spherotech ...
-
No products found
because this supplier's products are not listed.
Anna Spier, et al.,
bioRxiv - Cell Biology 2020
Quote:
For immune-labeling on thawed cryo sections cells were fixed with 2% PFA (EMS) + 0.1% glutaraldehyde (Sigma ...
-
No products found
because this supplier's products are not listed.
Rafal Donczew, et al.,
bioRxiv - Molecular Biology 2019
Quote:
Newly synthesized RNA isolated via 4-thioU labeling and purification was prepared for sequencing using the Ovation SoLo or Ovation Universal RNA-seq System kits (Tecan) according to the manufacturer’s instructions and 1 ng (SoLo ...
-
No products found
because this supplier's products are not listed.
Zhou Zhong, et al.,
bioRxiv - Microbiology 2020
Quote:
... alongside a protein molecular weight marker (BLUEstain protein ladder, Gold Biotechnology). Gels were run at 100 V for 15 min and then 150 V for 40 min in NuPAGE MES SDS running buffer and the proteins were subsequently transferred onto PVDF or nitrocellulose membranes using iBlot transfer stacks (Thermo Fisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Alejandra Arias-Cavieres, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Protein content was determined using a Protein Determination Kit (Cayman Chemical, Cat# 704002) and absorbance was measured at 595 nm using a Nanodrop 2000 spectrophotometer (Thermofisher ...
-
No products found
because this supplier's products are not listed.
Jürgen G. Okun, et al.,
bioRxiv - Physiology 2020
Quote:
... or protein-enriched (80%E protein, 10% carbohydrate, 10% fat; D16080303, Research Diets, USA) diets for a period of 3 weeks with various metabolic phenotyping tests ...
-
No products found
because this supplier's products are not listed.
He Liu, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
The cytoplasmic and nucleic protein were extracted separately using the protein extraction kit (Boster) according to the manufacture’s introduction ...
-
No products found
because this supplier's products are not listed.
Jingqi Zhou, et al.,
bioRxiv - Genomics 2021
Quote:
Zebrafish’s bone morphogenetic protein (BMP) and bone morphogenetic protein receptor genes were used as query to search the BMP and BMP receptor gene families in P ...
-
No products found
because this supplier's products are not listed.
Minghui Li, et al.,
bioRxiv - Pathology 2021
Quote:
... or IFNα with FITC- or Alexa488-conjugation (Biolegend or Bioss). The neutrophil marker Alexa-647-labeled anti- ly6G was used ...
-
No products found
because this supplier's products are not listed.
Evgeniy Panzhinskiy, et al.,
bioRxiv - Physiology 2021
Quote:
For pulse labeling of newly translated proteins MIN6 cells were incubated in complete DMEM media but without cysteine and methionine (MP Biomedicals, #SKU 091646454) for 1hr ...
-
No products found
because this supplier's products are not listed.
Clara-Marie Gürth, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Turnover of synaptic vesicles in mature cultures (DIV 15-25) was determined by live labeling with Atto647N-labeled mouse antibody against the luminal domain of synaptotagmin-1 (Synaptic Systems, cat ...
-
No products found
because this supplier's products are not listed.
Mayumi K. Holly, et al.,
bioRxiv - Microbiology 2020
Quote:
... C-reactive protein (MyBioSource). The optical density was read at 450 nm on an EnVision plate reader (PerkinElmer) ...
-
No products found
because this supplier's products are not listed.
Belen Esquerra-Ruvira, et al.,
bioRxiv - Molecular Biology 2022
Quote:
NZYBlue Protein Marker (NZYtech) was used for protein size estimation and protein concentrations were measured with QUBIT® 2.0 (Invitrogen).
-
No products found
because this supplier's products are not listed.
Veronica Gonzalez, et al.,
bioRxiv - Genomics 2021
Quote:
... 1.6×105 cells were suspended in P3 Primary Cell Solution with 50pmol Cas9 protein (St. Jude Protein Production Core) and 150pmol sgRNA (Synthego) with pMaxGFP plasmid at 200ng/ul (Lonza) in a total volume of 20ul with program DS130 ...
-
No products found
because this supplier's products are not listed.
Elaine C. Chen, et al.,
bioRxiv - Immunology 2021
Quote:
... Expressed protein was incubated with BioLock (IBA Lifesciences) and then isolated by Strep-tag affinity chromatography on StrepTrap HP columns (GE Healthcare) ...