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Powassan Virus NS1 Protein
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Genetex
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... cells were incubated with the anti-Influenza A virus NS1 rabbit antibody (GeneTex; GTX125990) at 1:1000 for two hours ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: ... it was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248). -
Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... NS1 (Invitrogen Cat #PIPA532243, 1:1000), NP (BioRad Cat#MCA400 ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in Journal of Virology doi: 10.1128/jvi.01985-18Quote: Interactions between the NS1 and the CCC proteins were detected by Duolink proximity ligation assay (PLA) Kit (Sigma Aldrich) in DENV4-infected mosquito cells (MOI=3) ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416, Abcam, WB 1:1000 ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... The virus (Addgene plasmid pAAV-hSyn-Flpo ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... Virus RNA virus was extracted by Qiamp viral RNA (Qiagen) and quantified using Qbit 3 Fluorometer (Thermo Fisher Scientific ... -
GenScript
No products found because this supplier's products are not listed.Cited in The secretory fate of flavivirus NS1 in mosquito cells is influenced by the caveolin binding domainbioRxiv - Molecular Biology 2019Quote: The NS1 sequence from ZIKV (Mexican isolate, Asiatic linkage) and YFV NS1 (Brazilian yellow fever virus isolate) were synthesized de novo (GenScript, Piscataway, NJ). The synthetic ZIKV NS1 gene (GenBank accession number KY631493.1 ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2018, published in Molecular Biology of the Cell doi: 10.1091/mbc.e19-03-0183Quote: ... p-24 lenti virus titer kit (TaKaRa) was used. -
Promega
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... and NS1 (3′) sequences were determined from purified product cloned into the pGEM-T vector by TA-cloning (Promega) according to the manufacturer’s guidelines. -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: ... The JEV NS1 gene was PCR-amplified with Deep Vent polymerase (New England Biolabs) using forward primer OSV 389 and sequential reverse primers OSV 381 ... -
Charles River Labs
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... Rescued virus was then amplified via inoculation of virus into 10-day-old embryonated chicken eggs (Charles River) at 37 °C for three days. -
System Biosciences
No products found because this supplier's products are not listed.Cited in A rectal cancer model establishes a platform to study individual responses to chemoradiationbioRxiv - Cancer Biology 2019Quote: ... the virus media was concentrated using PEG-it Virus Precipitation Solution (SBI System Biosciences), and then the lentiviral particles resuspended in 300 µL PBS ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... Purified NS1 protein from D2Y98P or 16681 (10 ng/well) diluted in PBS was coated onto 96-well EIA plates (Corning costar) overnight at 4 °C ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... a short protein sequence expressed in the injected virus (1:400 rabbit anti P2A; ABS31, Merck, Darmstadt, Germany). In the third step ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... or LightCycler Multiplex RNA Virus Master (Roche). SYBR green based RT-qPCRs were performed using Luna Universal One-Step RT-qPCR Kit (NEB). -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in mBio doi: 10.1128/mBio.00939-17Quote: ... NS1 substrates were labeled with Tran35S label (Perkin Elmer, Waltham, MA) by incubation in methionine- and cysteine free media containing 10% dialyzed FBS for 20 min before the addition of 200 μCi/mL of Tran35S label ... -
Sartorius
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... Purified NS1 was then concentrated using Vivaspin columns (Sartorius). -
Drummond Scientific
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... and virus was injected using either a Nanoject (Drummond Scientific) and glass pulled pipette or a syringe pump (Harvard Scientific ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... Recombinant NS1-6xHis protein was further purified by gel filtration using an AKTA pure chromatograph (GE Healthcare, USA). An XK 16/60 Chromatographic Column ... -
Sino Biological
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... Virus particles were immunostained with rabbit anti-SARS-CoV-2 N protein (Sino Biological) or rabbit anti-GM1 Ab (Abcam ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Journal of Virology doi: 10.1128/JVI.01591-19Quote: ... and the vesicular stomatitis virus G protein (VSV-G) expression vector pVPack-VSV-G (Stratagene). After 72 hours ... -
Beckman
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ... -
World Precision Instruments
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... The virus was injected using with an injector (Nanoliter 2010, WPI) in the thalamus or cortex through a small skull incision. -
Macherey-Nagel GmbH
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: The serum levels of a total of 12 virus-related cytokines were measured by a bead-based immunoassay panel (Mouse Anti-Virus Panel, Cat No: 740622, Biolegend, USA). The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020Quote: ... Then virus particles and polybrene (sc-134220; Santa Cruz) at a concentration of 2 μg/ml were added to the plate ... -
Vector Biolabs
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2020Quote: ... Ad-RFP virus (Vector Biolabs) at the same titer were used as a control. -
Invivogen
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... of 5 μg of each mAb (αDEC-NS1, αDCIR2-NS1, αDEC or αDCIR2) together with 50 μg poly (I:C) (Invivogen), exactly as described in (17) ... -
Eppendorf
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ... -
VWR
No products found because this supplier's products are not listed.Cited in New factors for protein transport identified by a genome-wide CRISPRi screen in mammalian cellsbioRxiv - Cell Biology 2019, published in The Journal of Cell Biology doi: 10.1083/jcb.201902028Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: Virus injections of pupae were performed with insulin syringes (BD Micro Fine Plus ... -
Alpha Diagnostic
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Nature Communications doi: 10.1038/s41467-019-12295-2Quote: ... anti-ZIKV NS1-IgG (Alpha Diagnostics, San Antonio, TX, USA) at baseline ... -
Mirus Bio
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2021Quote: ... torque teno virus hypothetical protein or human respirovirus 3 D protein were transfected into the cells using TransIT-LT1 (Mirus Bio) according to manufacturer’s protocol ... -
Illumina
No products found because this supplier's products are not listed.Cited in Inhibiting LSD1 suppresses coronavirus-induced inflammation but spares innate antiviral activitybioRxiv - Immunology 2021Quote: ... The identity of the virus was confirmed by Illumina sequencing (see supplementary table 1). -
The Native Antigen Company
bioRxiv - Microbiology 2020Quote: ... 10 mg/kg of purified NS1(D2Y98P) (The Native Antigen Company) or OVA protein (InvivoGen ... -
KeraFAST
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... Pseudoviruses bearing mutant S proteins were produced using the ΔG luciferase recombinant vesicular stomatitis virus (rVSV) system (KeraFast EH1025-PM)(Whitt ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.Cited in Human, Nonhuman Primate, and Bat Cells Are Broadly Susceptible to Tibrovirus Particle Cell EntrybioRxiv - Microbiology 2018, published in Frontiers in Microbiology doi: 10.3389/fmicb.2019.00856Quote: ... virus-exposed cells were harvested and lysed using Cell Lysis Buffer (Cell Signaling Technology) with cOmplete Protease Inhibitor Cocktail (Sigma ... -
LI-COR
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... Virus replication foci were counted using the Image Studio Lite software (v5.2.5, LI-COR, USA), and titres were determined based on dilution factors and expressed as focus forming units per mL (FFU mL^−1). -
Cell Biolabs
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: ... Virus was produced by transfecting Platinum-E retroviral packaging cells (Cell Biolabs) according to the manufacturer’s protocol ... -
R&D Systems
No products found because this supplier's products are not listed.Cited in HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019, published in mBio doi: 10.1128/mBio.02221-19R1Quote: ... Virus titers were measured using p24 enzyme-linked immunosorbent assays (ELISA) (R&D Systems, MN, USA). CD4+ Jurkat T cells were incubated with NL4-3 or NL4-3dNef viruses at MOI ... -
Expedeon
No products found because this supplier's products are not listed.Cited in Safe and easy evaluation of tmRNA-SmpB-mediated trans-translation in ESKAPE pathogenic bacteriabioRxiv - Biochemistry 2020Quote: ... Proteins were detected using InstantBlue protein stain (Expedeon) according to the supplier’s instructions. -
Protein Simple
No products found because this supplier's products are not listed.bioRxiv - Genetics 2021Quote: ... Protein analyses were performed using Compass software (Protein Simple). All results were then normalized to α-actin abundance and presented relative to healthy age matched WT controls (C57BL/10). -
PNA Bio
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... Cas9 protein (PNA bio) and gRNA were incubated at room temperature for 15 min in a 3:1 reaction with 15μg Cas9 and 5000 ng gRNA (2500ng of sgRNA#1 and 2500ng of sgRNA#2).47 The incubation product (gRNA and Cas9 complex ... -
ChromoTek
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... Fluorescent signal from the virus was amplified by immunostaining against Red Fluorescent Protein (RFP) (catalog no.5F8, Chromotek GmbH, Germany), followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no ... -
Biovision
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... the PEG Virus Precipitation Kit (BioVision, cat #K904) was used ... -
Harvard Apparatus
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... Virus was injected using a syringe pump (Harvard Apparatus) fitted with a syringe (Hamilton ... -
Origene Technologies
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: ... virus particle containing supernatant was collected and enriched using LentiConcentrator (OriGene). Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich ... -
Electron Microscopy Sciences
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2018, published in eLife doi: 10.7554/eLife.36768Quote: ... Virus expression confirmation was performed using a BlueStar UV light (Electron Microscopy Sciences) at three days post injection ... -
Cytoskeleton
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018, published in Nature Communications doi: 10.1038/s41467-018-05840-yQuote: ... Protein concentration was measured using Precision Red protein assay reagent (Cytoskeleton, Inc.). ∼40 μg lysates were separated by SDS-PAGE and transferred onto the Immobilon P PVDF membrane (EMD Millipore) ...