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No products found
because this supplier's products are not listed.
Sung-Eun Choi, et al.,
bioRxiv - Pathology 2020
Quote:
... Total protein in the hydrolysed sample was also measured using the Total Protein Assay Kit (QuickZyme) and the relative amount of collagen per protein was analysed.
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No products found
because this supplier's products are not listed.
Ayan Rajgarhia, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... and protein extraction was carried out using a total protein extraction Kit (BioChain Institute, Inc. Hayward, CA). Protein concentration was evaluated using the Modified Lowry Protein Assay (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Donna Ye, et al.,
bioRxiv - Microbiology 2019
Quote:
... Media supplemented with milk protein (Hardy Diagnostics) was made by first sterilizing 2x media stocks (TSB or R2B ...
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No products found
because this supplier's products are not listed.
Yingyan Zhou, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
C1-INH protein levels were measured using the SPAplus automated protein analyzer (The Binding Site Group Ltd, Birmingham, UK) – which utilizes turbidimetric measurement to determine protein level – according to manufacturer’s instructions.
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No products found
because this supplier's products are not listed.
Paresh P Kulkarni, et al.,
bioRxiv - Cell Biology 2023
Quote:
Protein C activity in WT and Apoh-/- plasma was performed using the Chromogenix Coamatic Protein C activity kit (Diapharma). Briefly ...
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No products found
because this supplier's products are not listed.
Yuma Nishikawa, et al.,
bioRxiv - Synthetic Biology 2024
Quote:
... Protein was extracted from the cells using E.coli/Yeast Protein Extraction Buffer (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), and the lysate fraction was used for analysis.
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No products found
because this supplier's products are not listed.
Koichi Sato, Aiko G.M. Hendrikx, Puck Knipscheer,
bioRxiv - Biochemistry 2023
Quote:
... The wild-type protein was overexpressed as a N-terminal His6-tagged protein in the E.coli JM109(DE3) cells (Intact Genomics) cultured in 4.4 L LB medium and purified by the previously described method53 ...
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No products found
because this supplier's products are not listed.
J. Ignacio Gutiérrez, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 100 uM Gal4–VP16 (Protein One, P1019-02) and 100 uM ATP or AMP-PNP ...
-
No products found
because this supplier's products are not listed.
Liina Hannula, et al.,
bioRxiv - Microbiology 2022
Quote:
... RBD wt (aa 319-541 of the S protein) and S1 wt (aa 14-681 of the S protein) from Medix Biochemica; RBD single mutants K417N ...
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No products found
because this supplier's products are not listed.
Shunsuke Mori, Tetsuya Nakamura,
bioRxiv - Developmental Biology 2021
Quote:
... was used for RfxCas13d protein production (Bon Opus Biosciences, LLC). The final concentration of RfxCas13d was adjusted to 3 µg/µl in storage buffer (50 mM HEPES-KOH ...
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No products found
because this supplier's products are not listed.
Ugo Sardo, et al.,
bioRxiv - Physiology 2023
Quote:
Liver proteins were extracted by physical dissociation (Ultra-turrax, IKA) in PEB Buffer (150 mM NaCl ...
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No products found
because this supplier's products are not listed.
Megha Subramanian, et al.,
bioRxiv - Genomics 2022
Quote:
... TTR protein levels were measured with mouse prealbumin kit from ALPCO, 41-PALMS-E01 (serum diluted 1:4000) ...
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No products found
because this supplier's products are not listed.
Gozde S. Demirer, et al.,
bioRxiv - Plant Biology 2022
Quote:
... DNA sequences for these protein coding regions were synthesized by Bionexus™ into a GATEWAY™ compatible vector pUC57 with point mutations made to convert a proline to a lysine in Solyc04g079980 (P243-> L ...
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No products found
because this supplier's products are not listed.
Dennis S. Metselaar, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... glial fibrillary acidic protein (GFAP) (1:500; BT46-5002–04, BioTrend), S100 (1:1000 ...
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No products found
because this supplier's products are not listed.
Alicia Kairouani, et al.,
bioRxiv - Plant Biology 2023
Quote:
... proteins were extracted using a Constant cell disrupter system (Constant Systems) with a disruption pressure of 2.35kbar and then purified using His-bind buffer kit (Millipore ...
-
The herbicide resistance gene (bar gene) is currently the most commonly used herbicide...
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Leander Rohr, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Proteins were transiently expressed using the AGL1 Agrobacterium tumefaciens strain (Lifeasible), as previously described (Hecker et al. ...
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No products found
because this supplier's products are not listed.
Nicholai M. Hensley, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... Varying volumes of concentrated protein solution and luciferin assay mix (Targeting Systems; prepared to manufacturer’s specifications but with unknown concentration for mammalian cells ...
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No products found
because this supplier's products are not listed.
Clay D. Jackson-Litteken, et al.,
bioRxiv - Microbiology 2022
Quote:
... Purified protein was then used by Antibody Research Corporation (St. Peters, MO) to generate polyclonal rabbit antibody.
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No products found
because this supplier's products are not listed.
Richard Samuel Edward Babicz, et al.,
bioRxiv - Physiology 2022
Quote:
... Protein phosphorylation was visualized using autoradiography (Hyblot ES, Thomas Scientific, Swedesboro, NJ).
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No products found
because this supplier's products are not listed.
Chance J. Cosgriff, et al.,
bioRxiv - Microbiology 2019
Quote:
... Precipitated proteins were washed with 1 mL of 100% ethanol (Decon Laboratories Inc.) and incubated at 4°C for 30 minutes ...
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No products found
because this supplier's products are not listed.
Tsuyoshi Hattori, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The neurons were cultured with or without SPARCL1 recombinant protein (80 nM, ATGP3891, NKMAX) from day 8 to day 14 as previously done (34) ...
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No products found
because this supplier's products are not listed.
Hsiao-Wei Tsao, et al.,
bioRxiv - Immunology 2021
Quote:
... The following primary antibodies were used to detect designated proteins: Batf (Brookwood Biomedical, PAB4003), Irf4 (Santa Cruz ...
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No products found
because this supplier's products are not listed.
Sarah G. Seman, et al.,
bioRxiv - Immunology 2024
Quote:
... These beads were either coated with purified protein derivative (PPD, AJ Vaccines, Copenhagen, Denmark) or left uncoated ...
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No products found
because this supplier's products are not listed.
Paula Rodrigues de Almeida, et al.,
bioRxiv - Microbiology 2019
Quote:
Monoclonal antibody against ZIKV Non-Structural 1 (NS1) protein (Arigo Biolaboratories, Taiwan, Republic of China) was diluted 1:1000 in (PBS ...
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No products found
because this supplier's products are not listed.
Shafali Gupta, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Rabbit antibodies against the following proteins were used: GAPDH (Trevigen 2275-PC-100; 1:5000), p114 RhoGEF (Abcam ab96520 ...
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No products found
because this supplier's products are not listed.
Jack L. Scanlan, et al.,
bioRxiv - Genetics 2020
Quote:
... melanogaster EcKL protein sequences as queries and were annotated in Artemis (Carver et al., 2012). Gene model annotation was performed by reciprocally performing BLASTP and TBLASTN searches (Altschul et al. ...
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No products found
because this supplier's products are not listed.
Mengqi Luo, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Membranes with protein samples subsequently reacted with anti-ACTB (1:5000, pAb, ZEN BIO, China), anti-SCRN2 (1:500 ...
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No products found
because this supplier's products are not listed.
Aniuska Becerra-Artiles, et al.,
bioRxiv - Immunology 2022
Quote:
... and MHC proteins were removed using a Vydac C18 microspin column (The Nest Group, Ipswich, MA). The mixture of MHC and peptides were bound to the column ...
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No products found
because this supplier's products are not listed.
Nils H. Rustmeier, et al.,
bioRxiv - Biochemistry 2023
Quote:
... proteins were incubated with oligosaccharides (Forssman antigen trisaccharide: Elicityl, France; Blood group A trisaccharide: Biosynth, UK) for 5 minutes prior to their addition to the cellular samples (∼1×106 cells in 100 µL) ...
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No products found
because this supplier's products are not listed.
Leticia R. Q. Souza, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the primary antibodies [Non-structural protein 1 from ZIKV (NS1, 1:500, BioFront Technologies, BF1225-06;), Class III β-tubulin (TuJ3 ...
-
No products found
because this supplier's products are not listed.
Matthew R. McFarland, et al.,
bioRxiv - Systems Biology 2020
Quote:
... HA-tagged Gln4 protein was detected using an anti-HA antibody (HA.11 clone 16B12, Cambridge Biosciences) and SuperSignal West Femto substrate (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
Md. Abu Sayeed, et al.,
bioRxiv - Immunology 2022
Quote:
The purified recombinant ICP1 bacteriophage proteins were used to raise mAbs via a commercial vendor (ProMab Biotechnologies, Inc.) using a conventional hybridoma technique (28) ...
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No products found
because this supplier's products are not listed.
Marie S. Prevost, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Viral titres were estimated by quantification of the p24 capsid protein using HIV-1 p24 antigen immunoassay (ZeptoMetrix) and by Fluorescence Activated Cell Sorting (FACS).
-
No products found
because this supplier's products are not listed.
Eugene Kim, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and for acute phase protein α-2-macroglobulin using an ELISA kit (Life Diagnostics Inc., West Chester, USA).
-
No products found
because this supplier's products are not listed.
Alexandre P. Martin, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... S6 (effect of ERM proteins on Notch2 signaling) were acquired using a Prime 95B Scientific CMOS camera (Photometrics), giving different signal arbitrary units from the ones recorded with the QuantEMTM 512SC CCD camera used in the other experiments ...
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No products found
because this supplier's products are not listed.
A. Rahman, et al.,
bioRxiv - Immunology 2019
Quote:
... Lysates (containing protein at 6mg/mL) from four donors were pooled prior to Kinex antibody microarray analysis (Kinexus Bioinformatics) (H ...
-
No products found
because this supplier's products are not listed.
Luis F. Garcia-Alles, et al.,
bioRxiv - Biochemistry 2019
Quote:
... protein tags were removed by incubation overnight at 25°C with turbo TEV protease (GenWay, 5 μg/mL final) in 50 mM Tris pH 8.0 / 300 mM NaCl /2 mM DTT/ 1 mM EDTA ...
-
No products found
because this supplier's products are not listed.
Ye Bi, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Colorectal and pancreatic cancer cell lines were transduced using red fluorescent protein (RFP) and puromycin-encoding lentiviral particles obtained from GenTarget Inc ...
-
No products found
because this supplier's products are not listed.
Yafei Liu, et al.,
bioRxiv - Microbiology 2020
Quote:
A pME18S expression plasmid containing the full-length or subdomain spike protein was transiently transfected into HEK293T cells using PEI max (PolyScience); the pMx-GFP expression plasmids were used as the marker of transfected cells ...
-
No products found
because this supplier's products are not listed.
Sarah C. Donnelly, et al.,
bioRxiv - Microbiology 2022
Quote:
... samples containing 1–3 mg/mL of protein were evaluated using ICP-MS (Biotron Analytical Services, Western University, London, Canada). Briefly ...
-
No products found
because this supplier's products are not listed.
Irène Amblard, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Furimazin (1/500) was added to live cells and luciferase activity of the internalized protein was measured on a plate reader (Tristar, Berthold).
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No products found
because this supplier's products are not listed.
Michael Adams, et al.,
bioRxiv - Biochemistry 2021
Quote:
... with peptides generated from roughly 500ng of proteins injected for each analysis onto either 75 micron x 25cm packed emitter columns (New Objective) or 75 micron x 50 cm C18 Acclaim Pepmap columns (Thermo Scientific) ...
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No products found
because this supplier's products are not listed.
Martin F. Peter, et al.,
bioRxiv - Biophysics 2020
Quote:
... the protein eluate was treated with 5 times excess per cysteine of the nitroxide spin label MTSSL (Toronto Research Chemicals, Canada), dissolved in DMSO ...
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No products found
because this supplier's products are not listed.
Santhosh Gatreddi, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The precipitated protein was pelleted by centrifugation and the L-lactate concentration was measured using the D-/L-lactate assay kit (Megazyme Inc.), as previously described.3 To examine the reversibility of inactivation ...
-
No products found
because this supplier's products are not listed.
Tiberiu Loredan Stan, et al.,
bioRxiv - Genetics 2023
Quote:
Protein was isolated from muscle samples by homogenizing the muscles in 1.4 mm Zirconium Beads prefilled tubes (OPS Diagnostics; Lebanon, USA), containing 100 mM Tris-HCl (pH 6.8 ...
-
No products found
because this supplier's products are not listed.
Marina Johnson, et al.,
bioRxiv - Immunology 2020
Quote:
Samples were screened for IgG to SARS-CoV-2 N protein using a commercially available kit (Epitope Diagnostics Inc, San Diego, USA) as previously described (8).
-
No products found
because this supplier's products are not listed.
Takumi Koizumi, et al.,
bioRxiv - Biophysics 2024
Quote:
... cells expressing SNR-tagged proteins were simultaneously irradiated with light from a mercury lamp through a 540–580 nm bandpass filter (ET560/40x; Chroma Technology Corporation) and a 585 nm dichroic mirror (T585lpxr ...
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No products found
because this supplier's products are not listed.
Alireza Saidi-Mehrabad, et al.,
bioRxiv - Microbiology 2020
Quote:
... This vector and its product was targeted as the main contamination tracer in this study via PCR of vector sequences and macro-photography of the mNeonGreen protein fluorescence under 470 nm wavelength using a xenon arc lamp (Sutter Instruments; California, USA).
-
No products found
because this supplier's products are not listed.
Alberto Domingo López-Muñoz, et al.,
bioRxiv - Microbiology 2023
Quote:
... alone or in combination with purified recombinant proteins were placed in the lower chamber of a 96-well ChemoTx System plate (Neuro Probe # 101-5) in RPMI 1640 1 % FBS ...
-
No products found
because this supplier's products are not listed.
Rahul Kumar, et al.,
bioRxiv - Cell Biology 2023
Quote:
... T7-RILP proteins were expressed in Escherichia coli BL21 (500 μM isopropyl β-d-1-thiogalactopyranoside; Wisent Bioproducts; at room temperature for 16 hours) and purified using standard procedure in tris buffer [20 mM tris (pH 7.4) ...