-
No products found
because this supplier's products are not listed.
Jordana Griffiths, et al.,
bioRxiv - Immunology 2020
Quote:
... For domain binding determination PE-labelled secondary anti-mouse Fc antibody (Jackson Laboratories) was added ...
-
No products found
because this supplier's products are not listed.
Izumi Sasaki, et al.,
bioRxiv - Immunology 2023
Quote:
... to block Fc receptors and stained with biotinylated anti-mouse F4/80 (clone: Cl:A3-1, cat:MCA497B, BIO-RAD), PerCP-Cy5.5-conjugated streptavidin (551419 ...
-
No products found
because this supplier's products are not listed.
Bert Schepens, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... was captured on anti-mouse IgG (Fc specific) acceptor beads (Perkin Elmer, Cat nr. AL105C). Serial dilutions of antibodies and VHH-Fc (final concentration ranging between 100nM - 0,001 nM ...
-
No products found
because this supplier's products are not listed.
Miriam R. Fein, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Fc receptor blocker (Innovex Biosciences), and finally avidin/biotin blocking buffer (Vector Laboratories ...
-
No products found
because this supplier's products are not listed.
Sundary Sormendi, et al.,
bioRxiv - Immunology 2020
Quote:
BMDNs were obtained by crushing long bones the bones in 5% FCS using a mortar and either isolated by negative selection using the EasySep Mouse Neutrophil Enrichment Kit (Stemcell Technologies) or by positive selection using biotinylated antibodies (see supplemental data for more details).
-
No products found
because this supplier's products are not listed.
Kazuki Hattori, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The device was presoaked in FC-40 (3M) for more than 30 min and placed in a stage top incubator (STXG-WSKMX ...
-
No products found
because this supplier's products are not listed.
Vedavathi Madhu, et al.,
bioRxiv - Cell Biology 2024
Quote:
... For mouse primary antibody Mouse on Mouse Kit (Vector laboratories, BMK2202) was used for blocking and primary antibody incubation ...
-
No products found
because this supplier's products are not listed.
Cristina Lao-Peregrin, et al.,
bioRxiv - Cell Biology 2023
Quote:
HEK 293 cells were purchased from ATCC (CRL-1573) and authenticated by Bio-Synthesis, Inc ...
-
No products found
because this supplier's products are not listed.
Raphael Fettig, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... NIH-3T3 fibroblasts and HEK-293 cells (all from ATCC, LGC Standards GmbH, Wesel, Germany) were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Alexander P. Clark, et al.,
bioRxiv - Bioengineering 2021
Quote:
Human embryonic kidney cells 293 stably expressing human hyperpolarization-gated cyclic nucleotide-sensitive cation channel 1 (HEK-HCN1) were obtained from Charles River (CT6114). Cells were cultured and maintained according to the online protocol by Charles River ...
-
No products found
because this supplier's products are not listed.
Maxime Lorenzini, et al.,
bioRxiv - Physiology 2020
Quote:
Whole-cell NaV currents were recorded at room temperature from transiently transfected HEK-293 cells using an Axopatch 200A amplifier (Axon Instruments, Molecular Devices, San Jose, CA) 48 hours after transfection ...
-
No products found
because this supplier's products are not listed.
Can Bora Yildiz, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Cells were stimulated with 5 µg/mL of either the recombinant ephrinA5-Fc (#374-EA, Biotechne) or Fc protein (#109-1103, Rockland) as control ...
-
No products found
because this supplier's products are not listed.
Danila Boytsov, et al.,
bioRxiv - Biophysics 2022
Quote:
... the FCS unit (Confocor3, Carl Zeiss, Jena, Germany) of a commercial laser scanning microscope (LSM 510 ...
-
No products found
because this supplier's products are not listed.
Jiahe Lu, et al.,
bioRxiv - Immunology 2023
Quote:
... + 2.5μg/mL ICAM1-Fc (Novus Biologicals, #720-IC) was incubated overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Jeremy Krohn, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Primary antibodies were incubated overnight at 4°C in 2% BSA + 10% Fetal Calf Serum (FCS) + 0.3% Triton-X-100: mouse anti GFAP (GFAP; 1:4000; Synaptic Systems Cat# 173 011, RRID:AB_2232308), rabbit anti Mover (Mover ...
-
No products found
because this supplier's products are not listed.
Sonali A. Gandhi, et al.,
bioRxiv - Biophysics 2023
Quote:
FCS was performed with an inverted microscope (IX71, Olympus) that was customized to resolve up to four fluorophores as described previously.26 The optical setup included a super-continuum laser (SC-Pro ...
-
No products found
because this supplier's products are not listed.
Maurice Michel, et al.,
bioRxiv - Biochemistry 2024
Quote:
... goat anti-human IgG-Fc Alexa488 (Dianova, 1:400) or goat antihuman IgM Alexa 594 (Molecular Probes ...
-
No products found
because this supplier's products are not listed.
Mathieu Ferrari, et al.,
bioRxiv - Immunology 2021
Quote:
Enzymatic activity of active ACE2-Fc (ACRO biosystems – AC2-H5257) and ACE2(HH:NN)-Fc was measured by using Mca-APK(Dnp) (Enzo Life Science – BML-P163) as substrate in 96-well black microtiter plates ...
-
No products found
because this supplier's products are not listed.
Tomoaki Sobajima, et al.,
bioRxiv - Cell Biology 2023
Quote:
... CENP-A (mouse mAb, AbCam, ab13939; mouse mAb, GeneTex, GTX13939), CENP-C (guinea pig pAb ...
-
No products found
because this supplier's products are not listed.
Dorothee Jakob, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... mouse anti-KCNMA1 (Abnova), mouse monoclonal anti-Na+/K+-ATPase β1 subunit (Sigma ...
-
No products found
because this supplier's products are not listed.
Sandra Wimberger, et al.,
bioRxiv - Molecular Biology 2022
Quote:
HEK293T cells (parental clone HEK-293, ATCC CRL-1573; Genhunter corporation Q401) and HEK293T-KO cell pools (PARP1-/- ...
-
No products found
because this supplier's products are not listed.
Juan A. Ortega, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 40% confluent HEK-293 cells were transfected with HilyMax transfection reagent (Dojindo Molecular Technologies) according to manufacturer guidelines ...
-
No products found
because this supplier's products are not listed.
Jiuling Yang, et al.,
bioRxiv - Immunology 2024
Quote:
... Pvrig KO NK cells were isolated from spleens of Pvrig KO mice and transfected with two sgRNAs targeting mouse Tigit or Cd96 (IDT DNA) using Cas9-gRNA RNP-directed gene deletion on day 5 ...
-
No products found
because this supplier's products are not listed.
Maria B. Campaña, et al.,
bioRxiv - Biochemistry 2023
Quote:
Mammalian cell line HEK 293 (ATCC) was maintained in 75 mL vented tissue culture flasks (Celltreat) and kept to 70% confluency before splitting ...
-
No products found
because this supplier's products are not listed.
Cary R. Boyd-Shiwarski, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The homology arms were amplified from extracted HEK-293 cell genomic DNA (QuickExtract DNA extraction solution, Lucigen). The fidelity of all constructs was confirmed by Sanger sequencing prior to use.
-
No products found
because this supplier's products are not listed.
Magda Krakowczyk, et al.,
bioRxiv - Molecular Biology 2023
Quote:
HEK-293 cells (60%-70% of confluency) were fixed by 2.5% glutaraldehyde and 2% paraformaldehyde (both from Electron Microscopy Science, Inc.) solution for 1 h at 4°C ...
-
No products found
because this supplier's products are not listed.
Maike Pohlers, et al.,
bioRxiv - Cancer Biology 2023
Quote:
For automated dual luciferase reporter assay 2-2.5 x 104 HEK 293 T cells were seeded per 96-well by the liquid handling system epMotion 5075 (Eppendorf, Hamburg, Germany). HEK 293 T cells were transfected with 50 ng/well reporter vector with or without 3′UTR and 200 ng/well pSG5 empty vector or pSG5-miR-142-5p expression plasmid ...
-
No products found
because this supplier's products are not listed.
Aline Sardinha-Silva, et al.,
bioRxiv - Immunology 2024
Quote:
... and/or with 10 μg of both combined recombinant mouse IL-4-Fc and IL-13-Fc (5 μg of each) (Absolute Antibody) every other day for 10 days (5 treatments) ...
-
No products found
because this supplier's products are not listed.
Ruud H. Wijdeven, et al.,
bioRxiv - Immunology 2021
Quote:
... HEK 293T cells were transfected using polyethyleneimine (Polyscience Inc.) with packaging plasmids pRSVrev ...
-
No products found
because this supplier's products are not listed.
James E. Dennis, Taylor Splawn, Thomas J. Kean,
bioRxiv - Cell Biology 2019
Quote:
... Pseudolentiviral particles were made in HEK-293Ta cells (GeneCopoeia) by co-transfection of psPAX2 ...
-
No products found
because this supplier's products are not listed.
Liisa Nissinen, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... HEK and HOK (human oral keratinocytes) were from ScienCell Research Laboratories (Carlsbad ...
-
No products found
because this supplier's products are not listed.
Pablo Canales-Herrerias, et al.,
bioRxiv - Immunology 2023
Quote:
... Sections were stained with primary antibodies: Goat anti-mouse IgD (goat polyclonal GAM/IgD, FC/7S, Cedarlane Labs), biotin anti mouse GL7 (Biolegend) ...
-
No products found
because this supplier's products are not listed.
Henriette Hoffmann-Veltung, et al.,
bioRxiv - Immunology 2022
Quote:
... 100 µL of either goat anti-mouse IgG (for 6E2 hybridoma cells) or mouse anti-human IgG (for EBV-immortalized human B cells) specific for the Fc region (Mabtech) diluted in sterile PBS at 15 µg/mL were added to wells followed by incubation at 4°C for 24 hours ...
-
No products found
because this supplier's products are not listed.
Awadalkareem Adam, et al.,
bioRxiv - Immunology 2021
Quote:
... Human recombinant ACE2-Fc-tag (Raybiotech) was then added at 1 μg/mL and incubated overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Neha Upadhyay-Tiwari, et al.,
bioRxiv - Plant Biology 2024
Quote:
... with an autosampler (GILSON FC 203B). Buffer B was 200 mM ammonium formate with 90% (v/v ...
-
No products found
because this supplier's products are not listed.
Lesly Ibargüen-González, et al.,
bioRxiv - Cell Biology 2024
Quote:
... 6×106 HEK-293T cells were seeded in 10 cm plates (Sarstedt). The cells were transfected with 6 μg of psPAX2 ...
-
No products found
because this supplier's products are not listed.
Takahiro Yamada, et al.,
bioRxiv - Microbiology 2019
Quote:
... 2 % FCS (MP Biomedicals, Santa Ana, CA), 100 U/mL penicillin ...
-
No products found
because this supplier's products are not listed.
Yuyi Wang, et al.,
bioRxiv - Cancer Biology 2020
Quote:
HUVEC (Lifeline Cell Technology, Frederick MD; FC-0003), HDMEC (Clonetics ...
-
No products found
because this supplier's products are not listed.
Andrea Di Francesco, et al.,
bioRxiv - Genetics 2023
Quote:
... Antibodies including Fc block (clone 2.42, Leinco Technologies) were added and incubated for 30 minutes at 4°C ...
-
No products found
because this supplier's products are not listed.
Constanza Feliziani, et al.,
bioRxiv - Cell Biology 2020
Quote:
Triple IP3R knockout human embryonic kidney cell line (TKO-HEK) from Kerafast (Boston, MA, USA; #EUR030) was grown in DMEM (#11995-065 ...
-
No products found
because this supplier's products are not listed.
Susanna R. Bidgood, et al.,
bioRxiv - Microbiology 2020
Quote:
... 1% FCS for 30 min and immune-stained with anti-GFP nanobody (Chromotek) conjugated in-house to AlexaFluor647-NHS (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Julia E.J. Holm, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Forty-eight hours post transfection cells were removed and incubated in 250 μL/well of HEK Lysis Buffer containing protease Inhibitor Cocktail Set V (Calbiochem) before being centrifuged at 13 000 rpm for 10 min ...
-
No products found
because this supplier's products are not listed.
Tamina Lebek, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... and resuspended in 10% FCS in PBS with either DAPI (1 μM; Biotium, #40043) or DRAQ7 (300 nM ...
-
No products found
because this supplier's products are not listed.
Tara A. Gleeson, et al.,
bioRxiv - Immunology 2024
Quote:
... mouse-anti-mouse NLRP3 (1/1000 dilution; Cryo2, Adipogen, AG-20B-0014), rabbit-anti-mouse gasdermin-D (1/1000 dilution ...
-
No products found
because this supplier's products are not listed.
Maitreyi Rathod, et al.,
bioRxiv - Cell Biology 2023
Quote:
... mouse DSG1 ((#651111 Progen), rabbit keratin 10 (#905403 Biolegend) ...
-
No products found
because this supplier's products are not listed.
Mitsugu Shimobayashi, et al.,
bioRxiv - Physiology 2020
Quote:
Plasma Leptin levels were measured by mouse mouse Leptin ELISA kit (Crystal Chem) according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Eun Young Yu, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... mouse anti-GAPDH (ABclonal, AC002), mouse anti-TERT (SCBT ...
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... Mouse-anti-H2BK120ub (Active Motif). Then ...
-
No products found
because this supplier's products are not listed.
Federico Miozzo, et al.,
bioRxiv - Neuroscience 2021
Quote:
... mouse anti-TH (Immunostar 22941) 1:300 ...
-
No products found
because this supplier's products are not listed.
Ryan J. Duchatel, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... A Mouse C-peptide ELISA (ALPCO), was then used to quantify C-peptide levels in the plasma ...