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No products found
because this supplier's products are not listed.
Victoriane Peugnet, et al.,
bioRxiv - Cell Biology 2021
Quote:
... serine/threonine protein phosphatase inhibitors (Phosphatase inhibitor Cocktail 3, Sigma-Aldrich) and 1 mmol/L Na3VO4 ...
-
No products found
because this supplier's products are not listed.
Nathan A. McDonald, et al.,
bioRxiv - Neuroscience 2023
Quote:
... or phospho-serine/threonine (Abcam ab17464).
-
No products found
because this supplier's products are not listed.
Dhirendra Kumar Sharma, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... An antibody against phosphor serine/threonine epitope was procured commercially (Cell Signaling Technology, USA). Antibodies against RqkA of D ...
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No products found
because this supplier's products are not listed.
Ivan Martinez-Valbuena, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Serine 202 and Threonine 205 (AT8, 1:1,000 dilution, ref: MN1020, Thermo Scientific), Threonine 181 (AT270 ...
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No products found
because this supplier's products are not listed.
Charles A Berdan, et al.,
bioRxiv - Cancer Biology 2019
Quote:
FAK1 kinase domain (AA393-698) (Promega) (25 μg ...
-
No products found
because this supplier's products are not listed.
Gabriella O. Estevam, et al.,
bioRxiv - Molecular Biology 2023
Quote:
The kinase domain variant library was digested with PstI-HF (NEB) and NdeI-HF (NEB ...
-
No products found
because this supplier's products are not listed.
Anika Wiegard, et al.,
bioRxiv - Microbiology 2019
Quote:
... vectors containing the GAL4 activation domain (AD) and the GAL4 DNA-binding domain (BD) were used ...
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No products found
because this supplier's products are not listed.
Florencia P. Madorsky Rowdo, et al.,
bioRxiv - Cancer Biology 2023
Quote:
A human kinase domain-focused gRNA library (Addgene 117725) [25] was used to assess CRISPR Cas9 screening as a tool in breast cancer PDTO to identify whether novel vulnerabilities could be detected for each PDTO ...
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No products found
because this supplier's products are not listed.
Dennis M. Bjorklund, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... sBRAF kinase domain constructs were purified using glutathione affinity resin (Genscript, catalogue ...
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No products found
because this supplier's products are not listed.
Ying Fu, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... nanobodies were preincubated with 4 nM SARS-CoV-2 spike protein receptor binding domain fused to an Fc tag (RBD-Fc, SARS-CoV-2 spike protein residues 319-541) (Sino Biological, Wayne, PA) in PBS supplemented with 0.05 mg/mL BSA at 25°C for 30 minutes ...
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No products found
because this supplier's products are not listed.
Ying Xu, et al.,
bioRxiv - Bioengineering 2023
Quote:
... and Rho associated coiled-coil containing protein kinase 2 (Santa Cruz, sc-398519, 1:100). These antibodies were labeled with fluorophore-conjugated ...
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No products found
because this supplier's products are not listed.
Dina Aweida, Shenhav Cohen,
bioRxiv - Cell Biology 2022
Quote:
... anti-phospho-Threonine/Serine from ECM Biosciences, and anti-PLAA from Invitrogen ...
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No products found
because this supplier's products are not listed.
Natalya Pashkova, et al.,
bioRxiv - Cell Biology 2021
Quote:
... CALM ANTH domain and Ub proteins were further purified on a HiLoad 16/60 Superdex 200 column (GE Healthcare Biosciences, Pittsburg, PA) equilibrated with 20mM HEPES-NaOH ...
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No products found
because this supplier's products are not listed.
Nicolas Kint, Thomas Dubois, Patrick H. Viollier,
bioRxiv - Microbiology 2022
Quote:
... Protein-primary antibody complexes were revealed with horseradish peroxidase-labeled donkey anti-rabbit antibodies (Jackson ImmunoResearch, West Grove, PA) and ECL detection reagents (Amersham ...
-
No products found
because this supplier's products are not listed.
Jia Chen, et al.,
bioRxiv - Plant Biology 2024
Quote:
... for protein immunoblotting using anti-phospho-Serine/Threonine (ABclonal, AP0893) antibody.
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No products found
because this supplier's products are not listed.
Bryce W. Duncan, et al.,
bioRxiv - Neuroscience 2021
Quote:
... coiled-coil containing protein kinase (ROCK1/2) Y27632 (Tocris, Inc.) at 10 µM in PBS for 1-2 hr ...
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No products found
because this supplier's products are not listed.
Min Jin Lee, et al.,
bioRxiv - Pathology 2023
Quote:
... PBS containing 0.04% BSA (VWR, Radnor, PA) and for FACS ...
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No products found
because this supplier's products are not listed.
Natascha Tomazic, et al.,
bioRxiv - Biochemistry 2020
Quote:
... pASK-IBA7+ was from IBA Life Sciences, pET-Duet1 from Merck KgaA ...
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No products found
because this supplier's products are not listed.
Bianca M. Esch, et al.,
bioRxiv - Genetics 2020
Quote:
For serine incorporation assays using [13C315N1]-serine (Cambridge Isotope Labs) samples were eluted from a PepMap C18 easy spray column (Thermo ...
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No products found
because this supplier's products are not listed.
Daan Vorselen, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Membranes were incubated in the same buffer for 1 h under light agitation with the recombinant murine chimera proteins containing human Fc domains and the extracellular domains of TREM2 (R&D systems, 1729-T2-050, 0.1 mg/mL) or CD14 (R&D systems ...
-
No products found
because this supplier's products are not listed.
Susheel Sagar Bhat, et al.,
bioRxiv - Plant Biology 2019
Quote:
... For RNA Pol II phosphorylated at serine 2 in CTD domain rat 3E10 antibody was used (Chromotek), diluted 1:100 ...
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No products found
because this supplier's products are not listed.
Anouk Georges, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... We used the following primary antibodies: rabbit antibody against Protein Kinase Cα diluted at 1:500 (Antibody Registry ID: AB_477345, Merck), rabbit antibody against Recoverin at 1:1000 (AB_2253622 ...
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No products found
because this supplier's products are not listed.
Kinya Nomura, et al.,
bioRxiv - Plant Biology 2023
Quote:
... and a rabbit LexA binding domain antibody (Clontech), respectively.
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No products found
because this supplier's products are not listed.
Bert van de Kooij, et al.,
bioRxiv - Biochemistry 2019
Quote:
... The serine/threonine peptide library (Kinase Substrates Library, Group I and Group II, Anaspec) consists of 198 peptide pools with the sequence Y-A-Z-X-X-X-X-S/T-X-X-X-X-A-G-K-K-(LC-Biotin)-NH2 ...
-
No products found
because this supplier's products are not listed.
Jonathan M. Schmitz, et al.,
bioRxiv - Biochemistry 2022
Quote:
... constructs containing DNA encoding 8X-His-tagged CHI-domain-containing proteins were transformed into BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent) for recombinant protein expression ...
-
No products found
because this supplier's products are not listed.
Aastha Kumari, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 50 μl of 2X kinase assay buffer containing 40μM ATP (Roche) was added to the tubes and 10μg equivalent of corresponding lysate was added ...
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No products found
because this supplier's products are not listed.
Gaurav S Choudhary, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Protein A agarose beads ((Rockland Immunochemicals, Gilbertsville, PA) were added equally to all the samples and incubated for 1hr 4 °C ...
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No products found
because this supplier's products are not listed.
Tasha R Womack, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Rat antibody against Notch 3 intracellular domain was obtained from Biolegend. Blots were probed with a mouse anti-actin (ThermoFisher ...
-
No products found
because this supplier's products are not listed.
Gozde Basara, et al.,
bioRxiv - Bioengineering 2021
Quote:
... coiled-coil containing protein kinase (ROCK) inhibitor (5 μM, StemCell Technologies, Vancouver, BC, Canada). Until 95% confluency was reached ...
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No products found
because this supplier's products are not listed.
Gaowei Wang, et al.,
bioRxiv - Systems Biology 2023
Quote:
... and 20 µL Protein Kinase K (Qiagen) for 30 min at RT followed by the steps as described in the protocol of Dneasy Blood & Tissue Kit (QIAGEN) ...
-
No products found
because this supplier's products are not listed.
Ajay Kumar, et al.,
bioRxiv - Immunology 2021
Quote:
... 1μg of each protein (wild type, single domain, and domain deletion mutant) was immobilized onto NC membranes (0.2μ pore size; Bio-Rad). The membranes were kept for drying for 5-10 min at RT ...
-
No products found
because this supplier's products are not listed.
Shan-Shan Lin, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 160 nM WASP VCA domain protein (#VCG03, Cytoskeleton) and 60 nM Arp2/3 protein complex (#RP01P ...
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No products found
because this supplier's products are not listed.
Guillermina M. Luque, et al.,
bioRxiv - Cell Biology 2020
Quote:
... resuspended in PBS containing 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) for 10 min at room temperature ...
-
No products found
because this supplier's products are not listed.
Linh Le, et al.,
bioRxiv - Cell Biology 2020
Quote:
... pH 7.4] containing 0.5% glutaraldehyde (Polysciences, Warrington, PA) for 1–2 h at room temperature ...
-
No products found
because this supplier's products are not listed.
Marcelo N. Costa, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and 50 µM Rho-associated protein kinase inhibitor (ROCKi; Calbiochem, USA). This media was changed every other day for 6 days ...
-
No products found
because this supplier's products are not listed.
Quentin Bourgeois-Jaarsma, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Liposomes were formed by drying chloroform solutions containing 25% 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS, Avanti Polar Lipids) and 75% 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC ...
-
No products found
because this supplier's products are not listed.
Jonatan Alvelid, Martina Damenti, Ilaria Testa,
bioRxiv - Biophysics 2021
Quote:
... 1 μl of Synaptotagmin-1 antibody (1 mg/ml, luminal domain - Synaptic System, 105 3FB) and 1 μl of FluoTag-X2 anti-mouse KLC (batch n ...
-
No products found
because this supplier's products are not listed.
Laura Sichlinger, et al.,
bioRxiv - Neuroscience 2024
Quote:
... coiled-coil containing protein kinase (ROCK) inhibitor (ROCKi; Enzo Life Sciences, LKT-Y1000-M005) to a new plate ...
-
No products found
because this supplier's products are not listed.
Muhammed Jamsheer K, et al.,
bioRxiv - Plant Biology 2020
Quote:
... and incubated with 1.5 μg anti-SnRK1α1 antibody (SNF1-related protein kinase catalytic subunit alpha KIN10 antibody; catalog no.: AS10 919, Agrisera) in 600 μL PBS on a rotator shaker for 1 h at room temperature with gentle shaking ...
-
No products found
because this supplier's products are not listed.
Leyao Shen, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... L-[3H(G)]-Serine (PerkinElmer NET248250UC), L-[14C(U)]-Glycine (PerkinElmer NEC276E050UC) ...
-
No products found
because this supplier's products are not listed.
Xin Cui, et al.,
bioRxiv - Physiology 2020
Quote:
... were generated by crossing TRKC-floxed mouse model (fl/fl) where the TRKC kinase ATP-binding domain is flanked with loxP sites 48 (Jackson Laboratory #022364) with TH-Cre mice where Cre-recombinase expression is under the control of the sympathetic marker tyrosine hydroxylase (TH ...
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L-Threonine is an essential amino acid which is in its active form. It is important for the...
Cat# S4951, SKU# S4951-25mg,
25mg, $97.00
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Catarina Martins-Costa, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... with 50 μM Rho-associated protein kinase (ROCK) inhibitor (Selleck Chemicals, S1049). On day 3 ...
-
No products found
because this supplier's products are not listed.
Richard J Smith, et al.,
bioRxiv - Cell Biology 2019
Quote:
... rabbit α-pNDC80 Serine 55 (1:1000, GeneTex), guinea pig α-Cenp-C (BT20278 from Caltag + Medsystems) ...
-
No products found
because this supplier's products are not listed.
Vanessa M. Doulames, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Rho-associated protein kinase inhibitor (3 uM; 1293823; PeproTech) was added to the media for 1 day post replating.
-
No products found
because this supplier's products are not listed.
Romina J. Bevacqua, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Protein A-micrococcal nuclease (pA-MN; EpiCypher donation) was added to a concentration of 1:400 to nuclei ...
-
No products found
because this supplier's products are not listed.
Steven Pelech, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 500 nM (170 nM was used on some repeats to conserve material) of recombinant glutathione S-transferease (GST)-CRAF (Y340E/Y341E) kinase domain (sequence: 306-end) (SignalChem, cat# R01-13G); His-BRAF kinase domain (sequence ...
-
No products found
because this supplier's products are not listed.
Chuan Xu, et al.,
bioRxiv - Immunology 2023
Quote:
Rabbit polyclonal antibodies against human or murine IFNε proteins were generated by using peptides derived from IFNε protein sequences (Lampire Biological Laboratories (Pipersville, PA). The specificity of antibodies was determined by western blot analysis ...
-
No products found
because this supplier's products are not listed.
Nathaniel P Disser, et al.,
bioRxiv - Physiology 2019
Quote:
... followed by treatment with either the mitogen-activated protein kinase kinases 1 and 2 (MEK1/2) inhibitor PD98059 (50 μM; InvivoGen, San Diego, CA, USA) to block ERK1/2 activation ...
-
No products found
because this supplier's products are not listed.
Elisa Avolio, et al.,
bioRxiv - Cell Biology 2021
Quote:
... anti-Transmembrane Serine Protease 2 (TMPRSS2 - Proteintech 14437-1-AP, 1:100); anti-Neural/Glial antigen 2 (NG2 - Millipore AB5320 ...
-
No products found
because this supplier's products are not listed.
Seunghee Oh, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Recombinant Snf1 kinase domain (Snf1-KD) was phosphorylated by recombinant human CaMKK2 (Abnova) for its activation before kinase assays (Figure 5F) ...