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No products found
because this supplier's products are not listed.
Isaac L. Scott, et al.,
bioRxiv - Biophysics 2023
Quote:
... supplemented with 10% fetal calf serum (FCS) (Atlas Biologicals, Fort Collins, CO, USA) and 1x L-glutamine (Hyclone ...
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No products found
because this supplier's products are not listed.
Michihito Sasaki, et al.,
bioRxiv - Microbiology 2020
Quote:
... the original stock of this virus (wild type, WT) was prepared by inoculation of Vero-TMPRSS2 cells with Mynox mycoplasma elimination reagent (Minerva Biolabs) [11] ...
-
No products found
because this supplier's products are not listed.
Jianying Liu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Virus mixtures were subsequently mixed at a ratio of 1:1 with PBS-washed sheep blood cells (Hemostat laboratories, Galveston, TX, USA). Aliquots of blood meals were collected to verify the initial virus proportions in the inoculum ...
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No products found
because this supplier's products are not listed.
Swathi Jayaram, Merrit Romeike, Christa Buecker,
bioRxiv - Developmental Biology 2023
Quote:
... Cell pellets were resuspended in PBS to remove residual FCS and counted with a CASY cell counter (Biovendis). Labeling of cells with MULTI-Seq barcodes was performed as described34 ...
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No products found
because this supplier's products are not listed.
Gaurav Gupta, et al.,
bioRxiv - Immunology 2019
Quote:
... 4% mouse serum (ImmunoReagents) and 4% goat serum ...
-
No products found
because this supplier's products are not listed.
Michal Schwartz, et al.,
bioRxiv - Microbiology 2022
Quote:
... mouse anti-PP28 (EastCoast Bio), rabbit anti-GAPDH (Cell Signaling Technology) ...
-
No products found
because this supplier's products are not listed.
Rebecca O’Cleirigh, Roslyn Gibbs,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... containing 5% (v/v) FCS and incubated overnight in a humidified atmosphere at 37°C and 5% CO2 (Nuaire, DH Autoflow). After 24 hours 1.5mL of the media was removed and media with herb extract or media only control was added ...
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No products found
because this supplier's products are not listed.
Alisa Fox, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were washed and incubated in Accutase cell detachment solution for 10 min at room temperature to gently remove any surface-bound virus while preserving epitopes for flow cytometry analysis (Innovative Cell Technologies; as described in (39)) ...
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No products found
because this supplier's products are not listed.
Sung-Eun Choi, et al.,
bioRxiv - Pathology 2020
Quote:
... Total protein in the hydrolysed sample was also measured using the Total Protein Assay Kit (QuickZyme) and the relative amount of collagen per protein was analysed.
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No products found
because this supplier's products are not listed.
Deanna M. Marchionini, et al.,
bioRxiv - Neuroscience 2022
Quote:
... blocked in 10% normal goat serum/ 10% mouse- on-mouse blocking (ScyTek Laborities, No. MTM015)/ TBS ...
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No products found
because this supplier's products are not listed.
Lindsey M. Brier, Joseph P. Culver,
bioRxiv - Neuroscience 2021
Quote:
... The N=16 mice used for FC matrix computation had stainless steel EEG self-tapping screws (BASI Inc., West Lafayette, IN, USA) fixed at approximately -1mm posterior to bregma ...
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No products found
because this supplier's products are not listed.
Ayan Rajgarhia, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... and protein extraction was carried out using a total protein extraction Kit (BioChain Institute, Inc. Hayward, CA). Protein concentration was evaluated using the Modified Lowry Protein Assay (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Eric Waltari, et al.,
bioRxiv - Immunology 2019
Quote:
... Blocking solution (sciBLOCK Protein D1M solution, Scienion) was added at 200 μL/well with a multichannel pipet and allowed to incubate without agitation for 1 hour ...
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No products found
because this supplier's products are not listed.
Chun-Che Tseng, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Instant-Bands Protein Stain (EZBiolab, Parsippany, NJ) were used to visualize input and eluted material on a Typhoon FLA 7000 (GE Healthcare ...
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No products found
because this supplier's products are not listed.
Yingyan Zhou, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
C1-INH protein levels were measured using the SPAplus automated protein analyzer (The Binding Site Group Ltd, Birmingham, UK) – which utilizes turbidimetric measurement to determine protein level – according to manufacturer’s instructions.
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No products found
because this supplier's products are not listed.
Paresh P Kulkarni, et al.,
bioRxiv - Cell Biology 2023
Quote:
Protein C activity in WT and Apoh-/- plasma was performed using the Chromogenix Coamatic Protein C activity kit (Diapharma). Briefly ...
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No products found
because this supplier's products are not listed.
Koichi Sato, Aiko G.M. Hendrikx, Puck Knipscheer,
bioRxiv - Biochemistry 2023
Quote:
... The wild-type protein was overexpressed as a N-terminal His6-tagged protein in the E.coli JM109(DE3) cells (Intact Genomics) cultured in 4.4 L LB medium and purified by the previously described method53 ...
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No products found
because this supplier's products are not listed.
J. Ignacio Gutiérrez, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 100 uM Gal4–VP16 (Protein One, P1019-02) and 100 uM ATP or AMP-PNP ...
-
No products found
because this supplier's products are not listed.
Steven Swendeman, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Activated Protein C was obtained from Enzyme Research laboratories. For initial studies ...
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No products found
because this supplier's products are not listed.
Eli Fritz McDonald, et al.,
bioRxiv - Cell Biology 2021
Quote:
... purified from B lymphocyte hybridoma cells using a Recombinant Protein G - Sepharose 4B Affinity Column on an ÄKTA start protein purification system (GE Life Science Product # 29022094). Antibodies were used in immunoblotting buffer (5% bovine serum albumin (BSA ...
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No products found
because this supplier's products are not listed.
Sarah Schnabellehner, et al.,
bioRxiv - Cell Biology 2023
Quote:
... rabbit anti-mouse LYVE1 (Reliatech, 103-PA50AG; 1:200), rat anti-mouse EMCN (Santa Cruz Biotechnology ...
-
No products found
because this supplier's products are not listed.
M.M. Joglekar, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... VCAN (1:200, Mouse Anti-Versican Antibody 2B1, Seikagaku), and ELN (1:400 ...
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No products found
because this supplier's products are not listed.
Hope Dang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Actual mouse albumin concentrations were calculated by linear regression from a standard curve generated with mouse albumin standards (Kamiya Biomedical Company, Seattle, WA)42 ...
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No products found
because this supplier's products are not listed.
Justin Judd, Jonathan Lovas, Guo N. Huang,
bioRxiv - Developmental Biology 2019
Quote:
... Proteins were then transferred to PVDF-Plus membranes (Spectrum Chemical) and blocked in 5% milk in TBST (0.1% Tween 20) ...
-
No products found
because this supplier's products are not listed.
Shunsuke Mori, Tetsuya Nakamura,
bioRxiv - Developmental Biology 2021
Quote:
... was used for RfxCas13d protein production (Bon Opus Biosciences, LLC). The final concentration of RfxCas13d was adjusted to 3 µg/µl in storage buffer (50 mM HEPES-KOH ...
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No products found
because this supplier's products are not listed.
Sofia Rossini, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... while recombinant human IDO1 protein was obtained by Giotto Biotech. Construct expressing murine Src was obtained from Origene.
-
No products found
because this supplier's products are not listed.
Zikou Liu, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Mouse RIPK3 rabbit polyclonal PSC-2283-c100 (Axxora (Pro Sci))
-
No products found
because this supplier's products are not listed.
Maria O. Levitin, et al.,
bioRxiv - Genetics 2022
Quote:
... and RPS6 (NSJ Bioreagents; 1:100, mouse anti-rabbit monoclonal). After incubation with primary antibodies ...
-
No products found
because this supplier's products are not listed.
Malachy Guzman, et al.,
bioRxiv - Genetics 2023
Quote:
... Each mouse was weighed with a analytical laboratory scale (Ohaus) immediately prior to recording ...
-
No products found
because this supplier's products are not listed.
Marisa Oliveira, et al.,
bioRxiv - Immunology 2020
Quote:
... and chicken interferon alpha (Yeast-derived Recombinant Protein, Kingfisher Biotech, Inc) were diluted in nuclease-free water (Ambion ...
-
No products found
because this supplier's products are not listed.
Alicia Kairouani, et al.,
bioRxiv - Plant Biology 2023
Quote:
... proteins were extracted using a Constant cell disrupter system (Constant Systems) with a disruption pressure of 2.35kbar and then purified using His-bind buffer kit (Millipore ...
-
No products found
because this supplier's products are not listed.
Sophie E. Cousineau, et al.,
bioRxiv - Microbiology 2022
Quote:
... mouse anti-HCV core (clone B2, Anogen MO-I40015B, 1:7,500); mouse anti-JFH-1 NS5A (clone 7B5 ...
-
No products found
because this supplier's products are not listed.
Drew A. Gillett, et al.,
bioRxiv - Immunology 2024
Quote:
Recombinant mouse GPNMB ECD (Aviscera Biosciences, product code 00719-03-10) was diluted in pre-warmed complete RPMI to a final concentration of 0.5ug/ml and 1.0ug/mL and was applied for 4 hours prior to lipopolysaccharide (LPS ...
-
No products found
because this supplier's products are not listed.
Abishek Chandrashekar, et al.,
bioRxiv - Microbiology 2022
Quote:
... or B.1.1.529 (Omicron; GISAID ID: EPI_ISL_7358094.2) Spike proteins (21st Century Biochemicals). 106 peripheral blood mononuclear cells well were re-suspended in 100 µL of R10 media supplemented with CD49d monoclonal antibody (1 µg/mL ...
-
No products found
because this supplier's products are not listed.
Nicholai M. Hensley, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... Varying volumes of concentrated protein solution and luciferin assay mix (Targeting Systems; prepared to manufacturer’s specifications but with unknown concentration for mammalian cells ...
-
No products found
because this supplier's products are not listed.
Sunada Khadka, et al.,
bioRxiv - Biochemistry 2022
Quote:
... hygromycin resistance marker and red fluorescent protein (RFP) were purchased from Cellecta. For transfection ...
-
No products found
because this supplier's products are not listed.
Mizuho Nosaka, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... tPA (Mouse tPA ELISA Kit, PA92, Oxford Biomedical Research, Rochester Hills, MI), uPA (Active mouse uPA Functional Assay Kit ...
-
No products found
because this supplier's products are not listed.
Amrita Srivastava, et al.,
bioRxiv - Immunology 2020
Quote:
Mouse ankles were fixed for 24 hours in buffered zinc formalin (Anatech Ltd), decalcified for 9 days in Shandon TBD-2 ...
-
No products found
because this supplier's products are not listed.
Karen M. Dunkerley, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Protein phosphorylation was monitored to completion using Phos-Tag AAL™ (NARD Institute Ltd) SDS–PAGE ...
-
No products found
because this supplier's products are not listed.
Swayam Prakash Srivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Urine albumin levels were assayed using a Mouse Albumin ELISA Kit (Exocell, Philadelphia, PA).
-
siRNA to inhibit GC expression using RNA interference
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15 nmol USD $340.0, 30 nmol USD $510.0
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Yingjuan Liu, et al.,
bioRxiv - Genetics 2020
Quote:
... Secondary antibodies used for IF were goat-anti-mouse H&L FITC (Cohesion Biosciences) or goat-anti-rabbit H&L FITC (Cohesion Biosciences) ...
-
No products found
because this supplier's products are not listed.
Carina C D Joe, et al.,
bioRxiv - Bioengineering 2021
Quote:
Residual host-cell protein (HCP) was quantified using the HEK293 HCP ELISA kit (Cygnus Technologies) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Catherine S. Millar-Haskell, et al.,
bioRxiv - Bioengineering 2022
Quote:
ECM proteins were covalently attached to PAA using the crosslinker Sulfo-SANPAH (Covachem; 13414-100). To begin ...
-
No products found
because this supplier's products are not listed.
Xi Chen, et al.,
bioRxiv - Bioengineering 2021
Quote:
... hFIX protein was detected by a goat-anti-hFIX antibody (1:2000; Affinity Biologicals, GAFIX-AP). Mouse-anti-β-actin antibody (1:5000 ...
-
No products found
because this supplier's products are not listed.
Aniuska Becerra-Artiles, et al.,
bioRxiv - Immunology 2022
Quote:
... and MHC proteins were removed using a Vydac C18 microspin column (The Nest Group, Ipswich, MA). The mixture of MHC and peptides were bound to the column ...
-
No products found
because this supplier's products are not listed.
Weigao Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 300 μL pure protein solution was added to a 1 mm pathlength quartz cuvette (Starna Cells, USA). The scanning wavelength starts from 300 nm and ends at 190 nm ...
-
No products found
because this supplier's products are not listed.
Amanda L. Peiffer, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Active protein was quantified by titrating in the known active site protease inhibitor FPR-CMK (Haematologic Technologies). To determine the KM the initial fluorescence data ...
-
No products found
because this supplier's products are not listed.
Takanori Eguchi, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Overexpressed proteins in the chromatin fraction was analyzed by western blotting using anti-MZF1 antibody (Assay Biotechnology) and anti-SCAND1 antibody (ab64828 ...
-
No products found
because this supplier's products are not listed.
Tsunaki Hongu, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... mice were intravenously injected with clodronate-liposome (100-150 μl per mouse, every other day, Liposoma BV). Short-term experiments were performed by treatment with clodronate-liposome on day 14 ...
-
No products found
because this supplier's products are not listed.
Xue-Wei Wang, et al.,
bioRxiv - Neuroscience 2019
Quote:
... the right optic nerve of the mouse was exposed intraorbitally and crushed with Dumont #5 fine forceps (Fine Science Tools) for 5 s at approximately 1 mm behind the optic disc ...