-
No products found
because this supplier's products are not listed.
Manon Gourdelier, et al.,
bioRxiv - Microbiology 2022
Quote:
... images of fluorescent VLP were acquired using the TIRF-Microscopy (Nikon) with the 100x objective ...
-
No products found
because this supplier's products are not listed.
Morgan McSweeney, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Videos of VLPs diffusing in AM were recorded with MetaMorph software (Molecular Devices, Sunnyvale, CA) at a temporal resolution of 66.7 ms ...
-
No products found
because this supplier's products are not listed.
Ellen Van Gulck, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 48h post transfection virus was collected and concentrated with PEG Virus Precipitation Kit (BioVision Inc, USA) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Xiaonan Zhang, et al.,
bioRxiv - Microbiology 2020
Quote:
... The blood samples from enrolled patients were tested for hepatitis B virus surface antigen (HBsAg) and hepatitis B virus e antigen (HBeAg) by Abbott AXSYM HBsAg (normal ...
-
No products found
because this supplier's products are not listed.
Pengxiang Chang, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was diluted in HBS-EP buffer (TEKnova) containing 10 μM oseltamivir carboxylate (Roche ...
-
No products found
because this supplier's products are not listed.
Joshua J. Sims, et al.,
bioRxiv - Genetics 2021
Quote:
... We measured reporter virus transduction activity on a luminometer (BioTek) using the Renilla Glo Kit (Promega ...
-
No products found
because this supplier's products are not listed.
Paul K. LaFosse, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected unilaterally with a stereotactic syringe pump (Stoelting; Wood Dale, IL) through a pulled glass pipette tip cut to an opening of 10–15 μm diameter ...
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No products found
Svenja Fritzlar, et al.,
bioRxiv - Microbiology 2023
Quote:
... virus was activated by adding 8μg/mL trypsin (TPCK treated, Worthington, Lakewood, NJ) in FCS-free media to samples in a 1:1 ratio ...
-
No products found
because this supplier's products are not listed.
Qiulong Yan, et al.,
bioRxiv - Microbiology 2020
Quote:
The DNA and RNA of virus were extracted by using TIANamp Virus DNA / RNA Kit (TIANGEN) according to the manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Marissa D. Acciani, et al.,
bioRxiv - Microbiology 2020
Quote:
... VLP-producing cells were treated with 20 μM Z-VAD-FMK (Enzo Life Sciences) 3 hours following VLP transfection.
-
No products found
because this supplier's products are not listed.
Ferdinand Roesch, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was diluted in RPMI-1640 (Genesee Scientific) media containing 2% FBS and 10 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Stuti Mehta, et al.,
bioRxiv - Molecular Biology 2022
Quote:
AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit (GeneCopoeia) was used to determine the efficiency of 8 different AAV serotypes in infecting the HCC1187 cell line ...
-
No products found
because this supplier's products are not listed.
Wenwei Li, et al.,
bioRxiv - Microbiology 2021
Quote:
... virus containing supernatants were clarified using a 0.45 µM PDVF filter (Pall Corp, NY, USA # 4614) and pelleted by ultracentrifugation on a 15% sucrose cushion before resuspension in culture media to achieve a 20X concentration over the original volume ...
-
No products found
because this supplier's products are not listed.
José-María Díez, Carolina Romero, Rodrigo Gajardo,
bioRxiv - Immunology 2020
Quote:
... Human Anti-SARS-CoV-2 Virus Spike 1 [S1] IgG ELISA Kit (Alpha Diagnostic Intl. Inc.), against S1 subunit spike protein ...
-
No products found
because this supplier's products are not listed.
Youssouf Sereme, et al.,
bioRxiv - Microbiology 2020
Quote:
... The virus-infected MRC5 cells were collected and centrifuged at 700 × g for 15 minutes (Eppendorf Centrifuge 5810R). Supernatant was filtered by serial filtration steps to eliminate cells and cellular debris ...
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No products found
because this supplier's products are not listed.
JF Sturgill, et al.,
bioRxiv - Neuroscience 2020
Quote:
... AAV virus (300nL volume) was then pressure injected 100nL/min via a glass pipette pulled (P-97 Sutter Instruments) from borosilicate capillaries (Drummond calibrated 5ul ...
-
No products found
because this supplier's products are not listed.
Kevin J. Kramer, et al.,
bioRxiv - Immunology 2021
Quote:
... values at the endpoint (48 h after incubation with the virus) were determined using the RTCA software version 2.1.0 (ACEA Biosciences Inc.). Results are expressed as percent neutralization in a presence of respective antibody relative to control wells with no CPE minus CI values from control wells with maximum CPE ...
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No products found
because this supplier's products are not listed.
Damon H. May, et al.,
bioRxiv - Immunology 2024
Quote:
... Parvovirus B19 VLP/VP1/VP2 Co-Capsid Recombinant protein was purchased from Raybiotech) were biotinylated at optimized biotin-to-protein ratios that generated biotinylated proteins with 1-3 biotin(s ...
-
No products found
because this supplier's products are not listed.
Ilva Liekniņa, et al.,
bioRxiv - Neuroscience 2023
Quote:
... EPEA-displaying VLPs were first biotinylated using biotin-PEG4-NHS ester (Jena Bioscience, CLK-B103), after which it was immobilised on a 4PCP-STA streptavidin chip to a final density of 58 pg/mm2 ...
-
No products found
because this supplier's products are not listed.
Sophie L. Winter, et al.,
bioRxiv - Microbiology 2022
Quote:
... Purified pHluorin-labelled VLPs were added to a glow-discharged μ-Slide 8 well dish (ibidi) at a protein concentration of 10 ng/μl and were allowed to settle for 20 min at RT ...
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No products found
because this supplier's products are not listed.
Šimon Borna, et al.,
bioRxiv - Immunology 2022
Quote:
... and AAV6 virus (Signagen laboratories, and in house produced (33))-mediated delivery of homologous template containing NGFR reporter gene under control of PGK promoter as described previously (33) ...
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No products found
because this supplier's products are not listed.
Nia Toshkova, et al.,
bioRxiv - Immunology 2023
Quote:
... measles virus mosaic hemagglutinin (viral proteins were obtained from Immune Technology, New York, NY). Plates were incubated with proteins usually diluted to 5 μg/ml in PBS ...
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No products found
because this supplier's products are not listed.
William B. Klimstra, et al.,
bioRxiv - Microbiology 2020
Quote:
... 2 ml/well of virus growth medium containing 0.1% (w/v) immunodiffusion agarose (MP Biomedicals) was added and incubation was continued for 96 hours ...
-
No products found
because this supplier's products are not listed.
Matthew T. Birnie, et al.,
bioRxiv - Neuroscience 2022
Quote:
Virus labeled sections were scanned under a 10x objective of a fluorescent microscope (Olympus BX 61) equipped with a high-sensitivity CCD camera (Nikon Eclipse E400) ...
-
No products found
because this supplier's products are not listed.
Grant M. Zane, et al.,
bioRxiv - Microbiology 2022
Quote:
AAV4 (and AAV5) VLP preparations were validated using the serotype-specific monoclonal antibodies ADK4 (Progen, 610147) and ADK5b (Origene ...
-
No products found
because this supplier's products are not listed.
Jason Neidleman, et al.,
bioRxiv - Immunology 2020
Quote:
... or Influenza Virus Control Peptide Pool (Anaspec). As a positive control for cytokine detection ...
-
No products found
because this supplier's products are not listed.
Flavia Chiuppesi, et al.,
bioRxiv - Immunology 2020
Quote:
... or vesicular stomatitis virus G (VSV-G, Aldevron), using FuGENE6 (Roche ...
-
No products found
because this supplier's products are not listed.
Matthew D. Lauver, et al.,
bioRxiv - Immunology 2022
Quote:
... Virus dilutions were combined 1:1 with 0.45% sheep erythrocytes (Innovative Research) and incubated overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Emily A. Hemann, et al.,
bioRxiv - Immunology 2022
Quote:
... this virus/serum mixture is incubated with 0.5% chicken red blood cells (Rockland) and hemagglutination inhibition was measured after 30 minutes.
-
No products found
because this supplier's products are not listed.
Andrii Slonchak, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus replication foci were counted using the Image Studio Lite software (v5.2.5, LI-COR, USA), and titres were determined based on dilution factors and expressed as focus forming units per mL (FFU mL^−1).
-
No products found
because this supplier's products are not listed.
M. Bormann, et al.,
bioRxiv - Microbiology 2021
Quote:
... the infectious virus was recovered by vortexing the carriers placed in plastic containers (SARSTEDT, Nümbrecht, Germany) filled with 2 mL DMEM for 1 minute ...
-
No products found
because this supplier's products are not listed.
Yang Yang, et al.,
bioRxiv - Microbiology 2019
Quote:
... Virus titers were measured using p24 enzyme-linked immunosorbent assays (ELISA) (R&D Systems, MN, USA). CD4+ Jurkat T cells were incubated with NL4-3 or NL4-3dNef viruses at MOI ...
-
No products found
because this supplier's products are not listed.
Lijun Cong, et al.,
bioRxiv - Microbiology 2021
Quote:
... prior to cell supernatants being collected for quantification of virus production by HIV-1 p24 ELISA (XpressBio).
-
No products found
because this supplier's products are not listed.
Meropi Aravantinou, et al.,
bioRxiv - Immunology 2020
Quote:
... Virus titer was determined in CEMx174 cells (ATCC, Manassass, VA) by p27 ELISA quantification (ZeptoMetrix, Buffalo, NY) and syncytia scoring after 14 days with the calculation method of Reed and Meunch ...
-
No products found
because this supplier's products are not listed.
John K. Mich, et al.,
bioRxiv - Neuroscience 2023
Quote:
Virus injection and optic fiber implantation surgery was performed in C57BL/6J mice (The Jackson Laboratory, #000664) at around P60 ...
-
No products found
because this supplier's products are not listed.
Joshua D. Powell, et al.,
bioRxiv - Microbiology 2024
Quote:
... NJ) and were seronegative to IAV antibodies by a commercial ELISA kit (Swine Influenza Virus Ab Test, IDEXX) prior to the start of the study ...
-
No products found
because this supplier's products are not listed.
Harold Marcotte, et al.,
bioRxiv - Immunology 2021
Quote:
... The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus (#3629-1, Mabtech AB). The peptides are 15-mers overlapping with 11 amino acids ...
-
No products found
because this supplier's products are not listed.
Ayat Zawawi, et al.,
bioRxiv - Immunology 2019
Quote:
... the VLP recombinant proteins were separated on 10% polyacrylamide gels and stained with instant blue protein stain (Expedeon).
-
No products found
because this supplier's products are not listed.
Joydeb Sinha, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Harvested virus was 0.4μM filtered (Celltreat 229749) and concentrated by centrifugation using a 100kdA cut-off PES protein concentrator (Pierce 88533 ...
-
No products found
because this supplier's products are not listed.
Bettina C. Schwab, et al.,
bioRxiv - Neuroscience 2020
Quote:
We also performed microstimulation mapping of VLa and VLp (biphasic pulses <200μA, 0.2ms- duration at 300Hz; Model 2100, A-M Systems). Consistent with previous reports ...
-
No products found
because this supplier's products are not listed.
Petra Štěrbová, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Cryo-EM images of the VLPs at pH 6.5 were recorded using a K2 camera (Gatan Inc., Pleasanton, CA, USA) in counting mode ...
-
No products found
because this supplier's products are not listed.
Angela Ma, et al.,
bioRxiv - Microbiology 2022
Quote:
... adenovirus (D3Ultra Respiratory Virus Screening & Identification kit, Quidel, San Diego, CA), and cytomegalovirus (D3 DFA Cytomegalovirus Immediate Early Antigen Identification kit ...
-
No products found
because this supplier's products are not listed.
Youssouf Sereme, et al.,
bioRxiv - Microbiology 2020
Quote:
... A poliovirus serology-positive control serum (Poliomyelitis virus kit, GenWay, San Diego, California, USA) was also used as a control ...
-
No products found
because this supplier's products are not listed.
Daniela Niemeyer, et al.,
bioRxiv - Microbiology 2021
Quote:
... and B.1.351 was assessed by a surrogate virus neutralization test (cPass Assay, Medac, Wedel, Germany) as described previously (Momsen Reincke et al. ...
-
No products found
because this supplier's products are not listed.
Paula Lagan, et al.,
bioRxiv - Microbiology 2023
Quote:
... Successful virus isolation was confirmed either by haemagglutination of 0.5% chicken blood cells (Envigo #S.B-0008) or by RRT-PCR.
-
No products found
because this supplier's products are not listed.
Pin-Ji Lei, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... we inoculated 1×105 PFU UV-inactivated PR8 influenza virus + 2μg Anti-CD40 (BioXCell, Cat: BE0016-2) + 2μg poly I:C (InvivoGen ...
-
No products found
because this supplier's products are not listed.
Xiaozhen Liu, et al.,
bioRxiv - Genetics 2022
Quote:
... and vesicular stomatitis virus G protein (VSV-G) plasmid using polyetherimide (PEI) (B600070, ProteinTech Group, Chicago, USA) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Gila Lustig, et al.,
bioRxiv - Microbiology 2019
Quote:
... Supernatant containing released virus was harvested two days post-transfection and filtered through a 0.45 micron filter(GVS) and stored in 0.5ml aliqouts at −80°C ...
-
No products found
because this supplier's products are not listed.
Yoshiaki Nishimura, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cells were grown to ~90% confluency in a 96-well plate prior to addition of serially diluted virus to achieve luminescence signals of 30,000-1,000,000 (BMG Labtech PolarStar Optima plate reader at maximum gain) ...
-
No products found
because this supplier's products are not listed.
Ke-Ming Xie, et al.,
bioRxiv - Microbiology 2024
Quote:
Total nucleic acids were extracted using the HP Virus DNA/RNA Kit (R6873; Omega Bio-Tek, Norcross, USA), and carrier RNA was not used during the process to avoid potential interference with sequencing results ...