-
No products found
because this supplier's products are not listed.
Carolyn M. Highland, et al.,
bioRxiv - Biochemistry 2022
Quote:
HIV-1 virus-like particles (VLPs) were produced by Lipofectamine 3000 (Thermo Fisher Scientific) transfection of 293FT cells (purchased from Invitrogen ...
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No products found
because this supplier's products are not listed.
Mona O. Mohsen, et al.,
bioRxiv - Immunology 2021
Quote:
The naturally packaged ssRNA in Qβ-VLPs was digested as following: Qβ(ssRNA)-VLPs were washed 3x with 20mM HEPES (Sigma-Aldrich) using Amicon ultra 0.5ml tubes 100kDa MWCO ...
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No products found
because this supplier's products are not listed.
Shirley Bikel, et al.,
bioRxiv - Genomics 2020
Quote:
The DNA of VLPs was extracted following the manufacture’s protocol for the QIAampMinElute Virus Spin Kit (QIAGEN). The resulting DNAs were quantified (Qubit ...
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No products found
because this supplier's products are not listed.
Molly Ohainle, et al.,
bioRxiv - Microbiology 2019
Quote:
... Simian immunodeficiency virus (SIV)−VLPs containing Vpx were produced by the transfection of 2.18 μg pSIV−Δpsi/Δenv/ΔVif/ΔVpr (Addgene #132928) and 0.31 μg pMD2.G plasmid ...
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No products found
because this supplier's products are not listed.
Mona Abed, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Lentivirus particles or VLPs were isolated using a Lenti-X Concentrator (321231, Clontech).
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No products found
because this supplier's products are not listed.
Jessica Devant, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... the VLPs were pelleted for 2 h at 40,000 rpm (Beckman TLA55) at 4°C and subsequently resuspended in PBS (pH 7.4) ...
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No products found
because this supplier's products are not listed.
Anabel Guedán, et al.,
bioRxiv - Microbiology 2021
Quote:
... VLP were treated with 20 units/mL RQ1-DNase (Promega) in 10mM MgCl2 for 1h at 37°C before infection ...
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No products found
because this supplier's products are not listed.
A. Schlör, et al.,
bioRxiv - Immunology 2022
Quote:
... VLPs were precipitated from the clarified supernatant by the addition of PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, USA) at a ratio of 1:10 ...
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No products found
because this supplier's products are not listed.
Yunlong Cao, et al.,
bioRxiv - Immunology 2022
Quote:
... G*ΔG-VSV virus (VSV G pseudotyped virus, Kerafast) is used to infect 293T cells (American Type Culture Collection [ATCC] ...
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No products found
because this supplier's products are not listed.
Rebecca P. Sumner, et al.,
bioRxiv - Immunology 2023
Quote:
... Sendai virus was from Charles River Laboratories ...
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No products found
because this supplier's products are not listed.
Janina Rahlff, et al.,
bioRxiv - Microbiology 2023
Quote:
... Prokaryotic cells and virus-like particles (VLPs) from site #1 – #3 were measured on a flow cytometer (BD Accuri C6 ...
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No products found
because this supplier's products are not listed.
Takayuki Hishiki, et al.,
bioRxiv - Microbiology 2022
Quote:
... Rabbit anti-vaccinia virus antibody (Abcam) and anti-rabbit Alexa Fluor Plus 555 (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Tommy Tong, et al.,
bioRxiv - Immunology 2023
Quote:
VLPs were denatured by heating in 2xLaemmli buffer containing 2-mercaptoethanol (Bio-Rad) for 10 minutes at 95°C ...
-
No products found
because this supplier's products are not listed.
Lee Sherry, et al.,
bioRxiv - Microbiology 2022
Quote:
... quantified PV VLPs were diluted in phosphate buffered saline (Corning 46-013-CM) to provide a uniform quantity of D antigen ...
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No products found
because this supplier's products are not listed.
Shai Zilberzwige-Tal, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The supernatant containing Riboswitch-VLPs was filtered through a 0.22-μm syringe filter (Merck).
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No products found
because this supplier's products are not listed.
María Laura Kevorkian, et al.,
bioRxiv - Microbiology 2022
Quote:
... influenza virus hemagglutinin (Roche), followed by 1:4000 anti-rat horseradish peroxidase (HRP ...
-
No products found
because this supplier's products are not listed.
Kristina Lanko, et al.,
bioRxiv - Microbiology 2020
Quote:
Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
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No products found
because this supplier's products are not listed.
Ashley M Ngo, et al.,
bioRxiv - Microbiology 2019
Quote:
... Dengue virus NS2B (Genetex, GTX124246) at 1:1000 ...
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No products found
because this supplier's products are not listed.
Alexis S. Gorin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was injected iontophoretically (Nanoject II, Drummond Scientific) via a glass micropipette (tip diameter ∼15-20 μm ...
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No products found
because this supplier's products are not listed.
Joseph M Gibbons, et al.,
bioRxiv - Microbiology 2019
Quote:
... Virus-like particles (VLPs) were produced by linear polyethylenimine 25K (Polysciences) transfection of HEK-293T ...
-
No products found
because this supplier's products are not listed.
H. Christian Hong, et al.,
bioRxiv - Molecular Biology 2021
Quote:
The surrogate virus neutralization test (sVNT) assay was performed using the SARS-CoV-2 surrogate virus neutralization test kit (GenScript, NJ, USA). Briefly ...
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No products found
because this supplier's products are not listed.
Shijian Zhang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Env solubilized from cell lysates and VLPs was denatured by boiling in denaturing buffer (New England Biolabs) for 10 minutes ...
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No products found
because this supplier's products are not listed.
Ana Barajas, et al.,
bioRxiv - Immunology 2023
Quote:
VLP-producing Expi293F cells were extracellularly and intracellularly stained with Allophycocyanin (APC) anti-Flag (DYKDDDDK) tag antibody (1:500) (Biolegend) and intracellularly stained with the Fluorescein isothiocyanate-labeled KC57 (anti-HIV-1 p24 ...
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No products found
because this supplier's products are not listed.
S.N. Freda, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected using a microsyringe pump (World Precision Instruments); >5 minutes elapsed before the pipette was moved from the ventral injection site to the more dorsal injection site ...
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No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Ad-RFP virus (Vector Biolabs) at the same titer were used as a control.
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No products found
because this supplier's products are not listed.
Lucy Gordon, et al.,
bioRxiv - Microbiology 2021
Quote:
... A 5 μg/ml concentration of biotinylated stable O1/Manisa/TUR/69 FMD VLP (61) (kindly provided by Alison Burman) was immobilised on streptavidin-coated biosensors (Sartorius UK Limited) for 900 s ...
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No products found
because this supplier's products are not listed.
Anshul Sinha, et al.,
bioRxiv - Microbiology 2021
Quote:
... Fixed VLPs were filtered onto 0.02 μm filter membranes in triplicate (Anodisc, GE Healthcare, USA) and were stained with SYBR-Gold (2.5X concentration) ...
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No products found
because this supplier's products are not listed.
Michael B. Fessler, et al.,
bioRxiv - Immunology 2022
Quote:
... The virus sequence was confirmed by Illumina sequencing and did not differ from the parental virus ...
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No products found
because this supplier's products are not listed.
Eleonora Sementino, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Then virus particles and polybrene (sc-134220; Santa Cruz) at a concentration of 2 μg/ml were added to the plate ...
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Recombinant Antigen
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Carmen Mirabelli, et al.,
bioRxiv - Microbiology 2021
Quote:
... HNoV GII.4 virus-like particles (VLPs) were purchased from The Native Antigen Company, Poly (I:C ...
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No products found
because this supplier's products are not listed.
Jonathan Burnie, et al.,
bioRxiv - Microbiology 2021
Quote:
The quantification of HIV-1 p24 capsid protein was performed in captured virus lysates and virus-containing supernatants with the high-sensitivity AlphaLISA p24 detection kit following the manufacturer’s (PerkinElmer) instructions ...
-
No products found
because this supplier's products are not listed.
Hiroki Fukunaga, et al.,
bioRxiv - Biophysics 2023
Quote:
... and purified using the AdEasy Virus Purification Kit (Agilent Technologies). After the differentiation of C2C12 cells into myotubes ...
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No products found
because this supplier's products are not listed.
Andrew B Janowski, et al.,
bioRxiv - Microbiology 2022
Quote:
... the virus RNA was isolated from the virus stocks using Direct-zol RNA MicroPrep (Zymo research) and used for RT-PCR and Sanger sequencing of the entire virus genome.
-
No products found
because this supplier's products are not listed.
M. Zeeshan Chaudhry, et al.,
bioRxiv - Microbiology 2021
Quote:
... the virus suspension was mixed 1:1 with peqGOLD TriFast (VWR). Following ...
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No products found
because this supplier's products are not listed.
Enrique Lin-Shiao, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Concentrated VLPs were resuspended in SE buffer (Lonza) and electroporated with 2-4pmol HDR template using a 4-D Nucleofector with pulse code CM-150 (Lonza) ...
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No products found
because this supplier's products are not listed.
Sophie L. Winter, Petr Chlanda,
bioRxiv - Biophysics 2021
Quote:
VLPs were plunge frozen in liquid ethane kept at - 183°C using GP2 (Leica). A suspension containing a mixture of 10 nm protein A coated colloidal gold (Aurion ...
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No products found
because this supplier's products are not listed.
Victoria L. Corbit, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Virus was injected using a syringe pump (Harvard Apparatus) fitted with a syringe (Hamilton ...
-
No products found
because this supplier's products are not listed.
Jorge C.G. Blanco, et al.,
bioRxiv - Microbiology 2021
Quote:
The VLPs were prepared by transfecting avian cells (ELL-0 from American Type Culture Collection) with cDNAs encoding the NDV M and NP proteins ...
-
No products found
because this supplier's products are not listed.
Irina V. Chestakova, et al.,
bioRxiv - Microbiology 2023
Quote:
... and H1N1 virus A/California/07/2009 (Sino Biological) using an arbitrary fluorescence cut-off of 6,000 ...
-
No products found
because this supplier's products are not listed.
Nicolas Mateos, et al.,
bioRxiv - Biophysics 2023
Quote:
... We generated VLPs as follows: 57 μL of Trans-IT reagent (Mirus) were added to 2 μg of pR8ΔEnv.2 ...
-
No products found
because this supplier's products are not listed.
Arthur Flohr Svendsen, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Virus was produced by transfecting Platinum-E retroviral packaging cells (Cell Biolabs) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Reza Khayat, et al.,
bioRxiv - Biophysics 2019
Quote:
5 uL of pCV2-2 VLP samples was applied to CF200-CU carbon film 200 mesh copper grids (Electron Microscopy Science) for 1 min and the grids were then washed with 200 µl of 50 mM of Na Cacodylate buffer and then strained immediately with 50 µl of 0.5% uranyl acetate for 1 min ...
-
No products found
because this supplier's products are not listed.
Robbert Boudewijns, et al.,
bioRxiv - Microbiology 2020
Quote:
... virus stocks were confirmed to be free of mycoplasma (PlasmoTest, InvivoGen) and other adventitious agents by deep sequencing on a MiSeq platform (Illumina ...
-
No products found
because this supplier's products are not listed.
Anthony J. Snyder, Andrew T. Abad, Pranav Danthi,
bioRxiv - Microbiology 2021
Quote:
... the virus-resistant cell population was transferred to fresh 150-mm dishes (Greiner Bio-One) and allowed to expand in growth medium supplemented with 2 μg/ml puromycin (InvivoGen ...
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No products found
because this supplier's products are not listed.
Heather Swann, et al.,
bioRxiv - Biophysics 2020
Quote:
... Negative stain electron microscopy was performed on VLPs by applying 3.5 uL of VLPs to a glow-discharged formvar-carbon coated EM grid (Ted Pella, Redding, CA) followed by two de-ionized water washes and staining with 1% uranyl acetate ...
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No products found
Yang Li, et al.,
bioRxiv - Immunology 2021
Quote:
PAOC cells were immortalized with Lenti-hTERT virus (ABM; cat# G200) following manufacturer’s instructions and clonal sorting/expansion ...
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No products found
because this supplier's products are not listed.
Jessica Devant, Götz Hofhaus, Grant Hansman,
bioRxiv - Microbiology 2019
Quote:
UNSW-2012 and CHDC-1974 VLPs (3 μl) were applied on freshly glow discharged Quantifoil holey carbon support films (R1.2/1.3; Quantifoil) and blotted for 18 seconds at 100% humidity at 10°C before been plunged in liquid ethane using an FEI Mark IV Vitrobot (Thermo Fischer Scientific) ...
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No products found
because this supplier's products are not listed.
Camilla Schinner, et al.,
bioRxiv - Cell Biology 2021
Quote:
... virus particle containing supernatant was collected and enriched using LentiConcentrator (OriGene). Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Qixiang He, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The virus titers were measured by flow cytometry assay (Expression Systems).
-
No products found
because this supplier's products are not listed.
Justin J. Botterill, et al.,
bioRxiv - Neuroscience 2021
Quote:
Virus was delivered using a 500nL Neuros Syringe (#65457-02, Hamilton Company) attached to the stereotaxic apparatus with a probe holder (#751873 ...