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Norovirus GII.4 VLP
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The Native Antigen Company Sponsored
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Millipore Sigma
No products found because this supplier's products are not listed.Cited in Fucoidan but not 2’-Fucosyllactose could inhibit human norovirus replication in zebrafish larvaebioRxiv - Microbiology 2020Quote: ... the bound NoV capsid proteins were detected with mouse anti norovirus GII (1:1000; NativeAntigen) and by adding horseradish peroxidase conjugated with goat anti-mouse IgG (1:1500; Sigma Aldrich). Horseradish peroxidase activity was detected with a TMB (3,3’,5,5’-tetramethylbenzidine ... -
The Native Antigen Company
bioRxiv - Microbiology 2021Quote: ... HNoV GII.4 virus-like particles (VLPs) were purchased from The Native Antigen Company, Poly (I:C ... -
Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... VLP-WT and VLP-cvD were expressed using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific) following manufacturer’s instructions ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... The presence of norovirus GII RNA was confirmed by droplet digital PCR (Bio-Rad, Hercules, California, USA) using the probes and primers that were described previously (Nasheri ... -
Beckman
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... The VLPs were concentrated by ultracentrifugation for 2 h at 31,000 rpm at 4 °C (Beckman SW-31Ti rotor), and then resuspended in 500 μL of Ex-Cell 405 medium ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... VLP were treated with 20 units/mL RQ1-DNase (Promega) in 10mM MgCl2 for 1h at 37°C before infection ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2020Quote: ... and non-deamidated (pure N373 wildtype) GII.4 P dimer species was achieved by cation exchange chromatography using a 6 ml Resource S column (GE Healthcare) at 6 °C ... -
Quantifoil Micro Tools
No products found because this supplier's products are not listed.bioRxiv - Pathology 2019, published in PLOS Pathogens doi: 10.1371/journal.ppat.1008619Quote: ... of the purified MNV infectious particles and HNV GII.3 VLP were placed onto R 1.2/1.3 Quantifoil grids (Quantifoil Micro Tools) coated with a thin carbon membrane that were glow-discharged using a plasma ion bombarder (PIB-10 ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Synthetic Biology 2021Quote: ... VLP fractions were pooled and concentrated using 100 kDa MW cut-off centrifugal filters (Amicon Ultra 4 ml, Merck). Protein concentrations were measured using the linearised Bradford method54 with Pierce Coomassie Protein Assay Kit (Thermo Scientific). -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: Norovirus cDNA samples were treated with 0.16 mg/mL RNase A (100 mg/mL, Qiagen) for 30 minutes at 37 °C ... -
Electron Microscopy Sciences
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2019, published in Virology doi: 10.1016/j.virol.2019.09.001Quote: 5 uL of pCV2-2 VLP samples was applied to CF200-CU carbon film 200 mesh copper grids (Electron Microscopy Science) for 1 min and the grids were then washed with 200 µl of 50 mM of Na Cacodylate buffer and then strained immediately with 50 µl of 0.5% uranyl acetate for 1 min ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: ... Western-blot analysis was performed using the following antibodies: polyclonal anti-VLP antibody ([81]; 1:10,000) and monoclonal anti-Pab1 (Abcam; 1:1000). Quantification of signals was performed using the ImageJ software. -
Takara Bio
No products found because this supplier's products are not listed.Cited in The Gag Protein PEG10 Binds to RNA and Regulates Trophoblast Stem Cell Lineage SpecificationbioRxiv - Molecular Biology 2019, published in PLOS ONE doi: 10.1371/journal.pone.0214110Quote: ... Lentivirus particles or VLPs were isolated using a Lenti-X Concentrator (321231, Clontech). -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: Env solubilized from cell lysates and VLPs was denatured by boiling in denaturing buffer (New England Biolabs) for 10 minutes ... -
Leica
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2021Quote: VLPs were plunge frozen in liquid ethane kept at - 183°C using GP2 (Leica). A suspension containing a mixture of 10 nm protein A coated colloidal gold (Aurion ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... HTLV-1 Gag expression in cells and VLPs in the cell culture supernatant was detected using a mouse monoclonal antibody HTLV-1 p24 (6G9) (Santa Cruz Biotechnology, TX) 1:3,000 and secondary antibody goat anti-mouse IgG StarBright Blue 700 (Bio-Rad ... -
Tocris
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2022Quote: 4-Hydroxytamoxifen (4-OHT) (Tocris Bioscience) was dissolved in corn oil (Millipore-Sigma ... -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2018, published in The EMBO Journal doi: 10.15252/embj.2018100158Quote: ... 5 ng interleukin (IL) 4 (IL-4, PeproTech) and 0.5 μg anti-cluster of differentiation (CD ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... 4 (BD Biosciences). Spores were removed and resuspended in sterile TX buffer (50 mM Tris pH 7.4 ... -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2021Quote: ... Concentrated VLPs were resuspended in SE buffer (Lonza) and electroporated with 2-4pmol HDR template using a 4-D Nucleofector with pulse code CM-150 (Lonza) ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018Quote: ... reporter VLPs were made by packaging the lentiGuide.mCherry vector [54] (a gift from Richard Young, AddGene plasmid #104375) with psPAX2 and pseudotyping with an amphotropic MLV envelope ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2021Quote: ... or 25 ng/ml Interleukin-4 (IL-4) (Biolegend) were used to stimulate M1 or M2 polarization ... -
Polysciences
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Journal of Virology doi: 10.1128/JVI.01591-19Quote: ... Virus-like particles (VLPs) were produced by linear polyethylenimine 25K (Polysciences) transfection of HEK-293T ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... and hematoxylin (4 minute incubation) and Bluing Reagent (4 minute incubation) counterstain (Roche, Ventana Medical Systems ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2022Quote: ... or AAVGo.1 VLPs (2.5 μg/ml) were incubated in ELISA plates (Corning Costar # 9018) in 100 mM NaHC03 (pH 9.6 ... -
VWR
No products found because this supplier's products are not listed.Cited in Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter micebioRxiv - Immunology 2021Quote: ... They were immersed in 4 mL of 4% paraformaldehyde (VWR), incubated at RT for 1 hour and finally incubated ON at 4°C to achieve full fixation ... -
Eppendorf
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018Quote: ... 4 °C (Eppendorf), cell pellets were either shock frozen and stored at −80°C for no longer than 6 months or immediately used for lysate production ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... and interleukin 4 (IL-4; 500 U/mL; R&D Systems). GM-CSF and IL-4 were replenished on day 2 and 4 ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... caspase-4 (4450S; Cell Signaling), caspase-5 (D3G4W ... -
World Precision Instruments
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: 1.5 mm outer diameter and 1.12 mm inner diameter borosilicate glass capillaries were purchased from World Precision Instruments (WPI# TW150-4). We optimized fabrication procedures to obtain micropipettes with 2 μm diameter tips ... -
Alfa Aesar
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2017, published in eLife doi: 10.7554/eLife.26957Quote: Cells were fixed in 4% paraformaldehyde (Alfa Aesar, 30525-89-4) for 10 minutes ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2022Quote: ... LMP1 (CS1-4; Dako), CD81 (B399 ... -
Ted Pella
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2020, published in Scientific Reports doi: 10.1038/s41598-020-78656-wQuote: ... Negative stain electron microscopy was performed on VLPs by applying 3.5 uL of VLPs to a glow-discharged formvar-carbon coated EM grid (Ted Pella, Redding, CA) followed by two de-ionized water washes and staining with 1% uranyl acetate ... -
Molecular Devices
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2022Quote: ... Videos of VLPs diffusing in AM were recorded with MetaMorph software (Molecular Devices, Sunnyvale, CA) at a temporal resolution of 66.7 ms ... -
GenScript
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in mBio doi: 10.1128/mBio.02202-19Quote: ... The GII.4 Sydney 2012 variant specific-mAbs (1C10, 6E6, 17A5, and 18G12) were developed from mice immunized with RockvilleD1 strain VLP (GenScript, NJ, USA). HBGA-blocking assays were performed using mutant and wild-type VLPs ... -
Cayman Chemical
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: ... P-(4-Methoxyphenyl)-P-4-morpholinyl-phosphinodithioic acid (GYY4137; 400μM; Cayman Chemical), pyridoxal phosphate (PLP ... -
ibidi
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2020Quote: 4×10^4 cells were seeded in wells of 8-well slides (Ibidi # 80826) in MEM without phenol red (Gibco # 51200038 ... -
Calbiochem
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: Bleomycin (Calbiochem 9041-93-4) -
Charles River Labs
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2020Quote: ... Age-matched mice that had been castrated (n=4), ovariectomized (n=4), or sham-operated (n=4 male, n=4 female) by Charles River Laboratories were subjected to the isoproterenol regimen described above ... -
Protean
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2021Quote: ... from Bio-Rad (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels ... -
Vector Labs
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... They were finally washed 4 × 15 min in RT 0.3% PBT and placed in VectaShield at 4 °C overnight before imaging (Vector Laboratories). trans-Tango and GRASP analyses required separate staining of neuropil and respective fluorophores due to different incubation times. -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Genomics 2020Quote: ... 64 RNA-Seq libraries (4 time points x 4 tissues x 4 biological replicates) were prepared using the TruSeq RNA Sample Preparation Kit (Illumina). Libraries were sequenced on Illumina Nova-Seq 6000 sequencing platform at the Australian Genome Research Facility (AGRF ... -
Worthington Biochemical
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004191, Bulk, Inquire AskbioRxiv - Immunology 2017, published in Nature Communications doi: 10.1038/s41467-018-02892-yQuote: ... we digested tissues with 4 mg/ml collagenase type 4 (Worthington, NJ), 0.8 mg/ml DispaseII ... -
The Jackson Laboratory
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: Eight transgene-positive (4 male and 4 female) and eight transgene-negative J20-C57BL/6J APP transgenic mice (4 male and 4 female; The Jackson Laboratory, #006293) all 6-months of age were used for this study ... -
Nikon
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2018Quote: ... a ×2 or ×4 (Nikon Plan Apo Fluor 2×/0.06 or 4×/0.13 objective ... -
Bio X Cell
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... and anti-IL-4 (BioXCell); for Th2 ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018Quote: ... 4 μCi γ32P-ATP (Perkin Elmer), 10 U T4 PNK (New England Biolabs) ... -
Miltenyi Biotec
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... IL-4 Catch Reagent (Miltenyi Biotec) in a final volume of 100 µL per 106 cells for 15 min on ice ... -
Biotek
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018, published in The Journal of Cell Biology doi: 10.1083/jcb.201805100Quote: ... Liberated 4-methylumbelliferone (4-MU) was measured with a Synergy 4 microplate reader (BioTek) at an excitation wavelength of 360 nm and an emission wavelength of 450 nm ... -
Sutter Instruments
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Developmental Cell doi: 10.1016/j.devcel.2019.09.019Quote: ... Lambda DG-4 lamp (Sutter Instruments), filter sets 49002 ET GFP(Chroma) ...