-
No products found
because this supplier's products are not listed.
Bruno Giotti, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... NK cells were analyzed by flow cytometry for expression of NKG2A using above mentioned protocol but with anti-human NKG2A PECy5 antibody (Biolegend).
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No products found
because this supplier's products are not listed.
Zhou Zhou, et al.,
bioRxiv - Immunology 2023
Quote:
HEK293 cells were transfected with pcTBK1-His or pcGSK3B-Flag plasmid using Lipofectamine 2000 (Invitrogen, Waltham, MA, US) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Hannah P. Gideon, et al.,
bioRxiv - Immunology 2021
Quote:
... NKG2A (Z199, Beckman Coulter and intracellular markers include ...
-
No products found
because this supplier's products are not listed.
Shan Jiang, et al.,
bioRxiv - Microbiology 2023
Quote:
... PhoQ-His and Flag-MgrB were detected with the anti-His and anti-Flag primary antibody (Sigma), respectively ...
-
No products found
because this supplier's products are not listed.
Yong Zhang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... via baculovirus and S protein (S1 and RBD, His tag) expressed in human embryonic kidney cells (HEK293) were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
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No products found
because this supplier's products are not listed.
Mélanie Bodnar-Wachtel, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... pcDNA3.1-Flag-His-ATM (Addgene 31985), pcDNA3.1-mCherry-NLRP3 ...
-
No products found
because this supplier's products are not listed.
Trang H. Pham, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Myc-Flag-human TEADs (OriGene) and V5-ubiquitin (generated in house ...
-
No products found
because this supplier's products are not listed.
Alexander Kuzin, et al.,
bioRxiv - Genomics 2021
Quote:
... human Hek293 DNA was purchased from Genscript. S ...
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No products found
because this supplier's products are not listed.
Fynn M. Hansen, et al.,
bioRxiv - Systems Biology 2020
Quote:
HEK293 (human, DMSZ, ACC 635) and U2OS (human, American Type Culture Collection [ATCC], HTB-96) cell were cultivated in DMEM (Gibco ...
-
No products found
because this supplier's products are not listed.
Anny Gravdal, et al.,
bioRxiv - Cell Biology 2020
Quote:
Human Embryonic Kidney 293 (HEK293) cells (Clontech Laboratories) were used for most experiments described in this study ...
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No products found
because this supplier's products are not listed.
Kun Meng, et al.,
bioRxiv - Cell Biology 2022
Quote:
... and 6×His-Flag-Ub was conducted using Ni-NTA agarose (Qiagen), following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Sriram Narayanan, et al.,
bioRxiv - Immunology 2020
Quote:
... NKG2A AlexaFluor700 (FAB1059N) (from R&D systems). All antibodies were titrated and used at optimal dilutions prior to panel integration ...
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No products found
because this supplier's products are not listed.
Joanne Durgan, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Recombinant His-tagged human ATG4B (Abcam, ab188707) was pre-treated with 10 mM DTT for 15 mins ...
-
No products found
because this supplier's products are not listed.
Yann Aquino, et al.,
bioRxiv - Genomics 2022
Quote:
... NKG2A FITC (clone REA110, Miltenyi Biotec), NKG2C APC (clone REA205 ...
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No products found
because this supplier's products are not listed.
Yadira M. Soto-Feliciano, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human HEK293 cells were maintained in DMEM (Corning) supplemented with 10% FBS (Gibco) ...
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No products found
because this supplier's products are not listed.
Subeena Sood, et al.,
bioRxiv - Immunology 2023
Quote:
HEK293 cells expressing human ACE2 and TMPRSS2a (Invivogen) were seeded at 20,000 cells/well in a gelatin-coated 96-well plate (Corning ...
-
No products found
because this supplier's products are not listed.
Caroline Mitiká Watanabe, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... NKG2A (clone 131411, BD), KIR3DL1 (clone DX9 ...
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No products found
because this supplier's products are not listed.
Magdalena Oroń, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
-
No products found
because this supplier's products are not listed.
Irina Buckle, et al.,
bioRxiv - Immunology 2022
Quote:
... human IL-12p70 (HEK293) (Peprotech) and 50ng/ml recombinant human IL-18 (MBL International ...
-
No products found
because this supplier's products are not listed.
Daniele Tedesco, et al.,
bioRxiv - Biophysics 2021
Quote:
... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
-
No products found
because this supplier's products are not listed.
Zachary R. Crook, et al.,
bioRxiv - Bioengineering 2024
Quote:
... biotinylated His-Avi-tagged human TfR (TfR cross-reactivity, ACROBiosystems TFR-H82E5); His-tagged mouse TfR (TfR cross-reactivity ...
-
No products found
because this supplier's products are not listed.
Tzu-Hsuan Chang, et al.,
bioRxiv - Immunology 2023
Quote:
Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...
-
No products found
because this supplier's products are not listed.
Jérôme Wahis, et al.,
bioRxiv - Neuroscience 2020
Quote:
... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
-
No products found
because this supplier's products are not listed.
Catherine M. Mageeney, et al.,
bioRxiv - Microbiology 2020
Quote:
... Primary antibodies (Anti-FLAG: Sigma, F3165; Anti-His: Cell Signaling Technologies, Danver, MA, 2366S) were used at 1:1000 ...
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No products found
because this supplier's products are not listed.
Marco Di Gioia, et al.,
bioRxiv - Immunology 2023
Quote:
... His-tagged recombinant human AKT1 (BPS Bioscience, Cat# 40003), AKT2 (BPS Bioscience ...
-
No products found
because this supplier's products are not listed.
Laura Alonso-Herranz, et al.,
bioRxiv - Immunology 2020
Quote:
HEK293 cells (Lonza) were cultured in DMEM (Lonza ...
-
No products found
because this supplier's products are not listed.
Yuichi Tsuchiya, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... HEK293 cells (4 × 106 cells/150 mm dish) were transiently transfected with pcDNA3-hFGF18-FLAG using PEI-MAX (Polysciences 24765). The culture medium was changed 24 hours after transfection ...
-
No products found
because this supplier's products are not listed.
Katarzyna Z Haza, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 5% CO2 for 24 h before transfection with a 4:1 DNA ratio of pCMV6-Affimer-tGFP and FLAG-ERK plasmids using Lipofectamine 2000 (SW620, HEK293, NCI-H460) or X-tremeGENE 9 (Roche; Panc10.05). After 24 h cells were serum starved for 1 h ...
-
No products found
because this supplier's products are not listed.
Nilabhra Mitra, Sanghamitra Dey,
bioRxiv - Plant Biology 2021
Quote:
Ni pull down experiments were performed using 3μl of His tagged OsSRT1 constructs (0.3μg/ul) and 1μg recombinant human Histone H3 (NEB) and mixed with 20 μl of Ni-NTA slurry (Qiagen) ...
-
No products found
because this supplier's products are not listed.
Neil D. Butler, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... HRP-conjugated 6*His His-Tag Mouse McAB was obtained from Proteintech. Oligonucleotides were purchases from IDT ...
-
No products found
because this supplier's products are not listed.
Shraddha Pai, et al.,
bioRxiv - Genomics 2021
Quote:
We downloaded publicly-available Hi-C data from human prefrontal cortex tissue23,24 (Illumina HiSeq 2000 paired-end raw sequence reads ...
-
No products found
because this supplier's products are not listed.
Pille Hallast, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries using 1.5 M human cells as input were generated with Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) following the manufacturer’s protocol with the following modification ...
-
No products found
because this supplier's products are not listed.
Ana F. Ferreira, et al.,
bioRxiv - Neuroscience 2023
Quote:
Human samples: qPCR experiments were conducted using the SensiFAST Probe Hi-ROX Master Mix (Bioline) and the TaqMan Gene Expression Assays (Applied Biosystems ...
-
No products found
because this supplier's products are not listed.
Laura Klasek, Kentaro Inoue, Steven M. Theg,
bioRxiv - Plant Biology 2020
Quote:
... anti-His (Santa Cruz), anti-OE33 (Chou et al. ...
-
No products found
because this supplier's products are not listed.
Chongyang Li, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Flag-PFDN2 and Flag-VIM) and the bacterial expression vectors pReceiver-B11 (His-NSMCE2 and His-PFDN2) were purchased from Genecopoeia, Inc ...
-
No products found
because this supplier's products are not listed.
Simona Selberg, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
-
No products found
because this supplier's products are not listed.
Masako Kohyama, et al.,
bioRxiv - Microbiology 2022
Quote:
... in which human ACE2 and TMPRSS2 are induced by tetracycline (HEK293-3P6C33 cells) with TransIT-LT1 Transfection Reagent (Mirus, Madison, WI, USA), following the manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Aaron Embry, et al.,
bioRxiv - Microbiology 2024
Quote:
... and HopAM1 and C-terminal Flag-tagged IpaH4 were generated by PCR amplification using primers containing Flag sequences and flanking SacII / PacI off pIB/V5-His templates using iProof DNA polymerase (Bio-Rad; Table S4) and cloned into the pDGOpIE2 vector ...
-
No products found
because this supplier's products are not listed.
Masaya Yamaguchi, et al.,
bioRxiv - Microbiology 2019
Quote:
Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial ...
-
No products found
because this supplier's products are not listed.
Allen G. Sanderlin, et al.,
bioRxiv - Microbiology 2022
Quote:
... and precipitated culture supernatants (equivalent to 2 mL of culture supernatant prior to precipitation) were analyzed by western blot using rabbit anti-FLAG and HRP-conjugated mouse anti-His (ABclonal #AE028).
-
No products found
because this supplier's products are not listed.
Lie Wang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
-
No products found
because this supplier's products are not listed.
Stefania Capone, et al.,
bioRxiv - Immunology 2020
Quote:
... recombinant human His-tagged ACE-2 protein (RayBiotech) was incubated on cells before adding any antibodies ...
-
No products found
because this supplier's products are not listed.
Brian L. Hie, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
-
Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1...
Cat# B26102, SKU# B26102-5 mL,
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Zhou Zhou, et al.,
bioRxiv - Immunology 2023
Quote:
... Immunoprecipitation was performed with anti-His or Flag magnetic beads (Bimake, Texas, US), according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Elizabeth D. Luczak, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... FLAG (Rockland), GAPDH (Cell Signaling) ...
-
No products found
because this supplier's products are not listed.
Kevin Michalski, et al.,
bioRxiv - Neuroscience 2019
Quote:
HEK293 cells were plated onto 12-mm glass coverslips (VWR) in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega ...
-
No products found
because this supplier's products are not listed.
Chang-Hoon Kim, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Recombinant Flag-tagged human G9a (Active Motif, 31410), human calpain 1 (Novus ...
-
No products found
because this supplier's products are not listed.
Junming Ren, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Anti-mouse NKG2A (20D5, purified) and anti-mouse CD28 (37.51, purified) were purchased from BioXcell. Anti-mouse Ly-49C and Ly-49I (5E6 ...
-
No products found
because this supplier's products are not listed.
Xi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or plastic-bottom (Ibidi: DLD1, HEK293) 96-well plates at densities targeting 60-70% confluence at the time of transfection ...
-
No products found
because this supplier's products are not listed.
Tim Nicholson-Shaw, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... FLAG peptide (ApexBio, A6002) was added to lysates to a concentration of 1 µg/ml to reduce non-specific interactions ...