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No products found
because this supplier's products are not listed.
Brenton T. Laing, et al.,
bioRxiv - Neuroscience 2022
Quote:
Immunostaining was conducted for axonal projections and verification of virus expression using a chicken anti-GFP polyclonal antibody (1:1000, Cat # GFP-1020, RRID:AB_10000240; Aves Labs, CA, U.S.A.) or a rabbit anti-DsRed polyclonal antibody (1:1000 ...
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No products found
because this supplier's products are not listed.
Michael J Massare, et al.,
bioRxiv - Immunology 2021
Quote:
... pBac1 plasmids were transfected into Sf9 with Flash-bacGOLD bacmid containing the Autographa californica polydedrosis virus genome (Oxford Expression Technology, Oxford UK). Sf9 cells cultures were infected with recombinant baculovirus expressing the HA genes ...
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No products found
because this supplier's products are not listed.
Riley A. Suhar, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 40- L of sample were transferred into disposable cuvettes (BrandTech, 759200) and sealed with a cap (VWR ...
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No products found
because this supplier's products are not listed.
Raphaëlle Delattre, et al.,
bioRxiv - Microbiology 2021
Quote:
... that was amplified on strain 536 and purified according to molecular biology protocols including an endotoxin removal step (Endotrap Blue, Hyglos, Germany). The stock solution of 8.2 log10 PFU/mL has an endotoxin concentration of 2.15 EU/mL.
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No products found
because this supplier's products are not listed.
Alisa Fox, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were washed and incubated in Accutase cell detachment solution for 10 min at room temperature to gently remove any surface-bound virus while preserving epitopes for flow cytometry analysis (Innovative Cell Technologies; as described in (39)) ...
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No products found
because this supplier's products are not listed.
Valentin Dunsing, et al.,
bioRxiv - Biophysics 2021
Quote:
... The mEGFP-(L)-mApple construct was cloned beforehand by amplifying mApple from PMT-mApple (39 ...
-
No products found
because this supplier's products are not listed.
Geetha Parthasarathy, et al.,
bioRxiv - Immunology 2022
Quote:
... Assay was conducted with RayBiotech custom L-series human array (RayBiotech, Peachtree corner, GA). The assay uses a semi-quantitative modified ELISA procedure wherein the proteins in the sample are directly labelled with biotin and used as a probe to bind corresponding antibodies printed on a glass slide ...
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No products found
because this supplier's products are not listed.
Lena Kleij, et al.,
bioRxiv - Microbiology 2023
Quote:
... and soybean L-α-phosphatidylinositol (PI) (37-0130-7) were purchased from Larodan (France). ANTS (FP-46574B ...
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No products found
because this supplier's products are not listed.
Karl Alex Hedin, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... for preventing bacterial growth and 1 g/L 5-Fluoroorotic Acid (5-FOA; Nordic BioSite) for preventing other yeast species from growing.
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No products found
because this supplier's products are not listed.
Poorani Ganesh Subramani, et al.,
bioRxiv - Immunology 2023
Quote:
... Samples were immunoprecipitated with 5 μg anti-hnRNP L antibody (Aviva ARP40368_P050. Lot: QC9464-42964) or control anti-IgG antibody (sc-2025 ...
-
No products found
because this supplier's products are not listed.
Shengjun Hong, et al.,
bioRxiv - Genomics 2020
Quote:
... NF-L levels were measured using a commercial ELISA (NF-light ELISA, UmanDiagnostics, Umeå, Sweden 18). Ng levels were measured using an in-house immunoassay for Ng 19 ...
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No products found
because this supplier's products are not listed.
Xien Yu Chua, Arthur Salomon,
bioRxiv - Biochemistry 2021
Quote:
... 2 mM L-Glutamine (HyClone) and 10% (v/v) heat-inactivated fetal bovine serum (Peak Serum), in a humidified incubator with 5% CO2 at 37°C ...
-
No products found
because this supplier's products are not listed.
Victoria L. Messerschmidt, et al.,
bioRxiv - Bioengineering 2021
Quote:
Poly(D, L-lactide-co-glycolic acid) nanoparticles (PLGA, 50:50, Akina Inc., West Lafayette, IN, USA) of two different molecular weights including 55–65 kDa (HMW Nanoparticles ...
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No products found
because this supplier's products are not listed.
Melissa A. Fischer, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and 1x L-GlutaMAX) and treated for 48 hours with an MCL1 inhibitor (S63845; Chemietek, Indianapolis, IN), BCL2 inhibitor (venetoclax ...
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No products found
because this supplier's products are not listed.
Dheva Setiaputra, et al.,
bioRxiv - Molecular Biology 2021
Quote:
µL PPB1 and 50 µL of 10 mg/mL HeLa nuclear extracts (Accurate Chemical, Carle Place NY) and incubated by rotating at 4°C ...
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No products found
because this supplier's products are not listed.
Alexandra Colin, et al.,
bioRxiv - Cell Biology 2022
Quote:
L-a-phosphatidylcholine (EggPC) (Avanti, 840051C) and ATTO 647N labeled DOPE (ATTO-TEC, AD 647N-161 dehydrated) were used ...
-
No products found
because this supplier's products are not listed.
Cathrin LC Gudd, et al.,
bioRxiv - Immunology 2023
Quote:
... 20 μg/mouse of TLR9-L (CpG oligodeoxynuleotide 1668: 5-S-TCCATGACGTTC CTGATGCT-3) (TIB Molbiol, Germany) was administered i.p ...
-
No products found
because this supplier's products are not listed.
Alex Macpherson, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and 48 µL of GVB0 buffer (0.1 % gelatin, 5 mM Veronal, 145 mM NaCl, 0.025 % NaN3, pH 7.3, Complement Technology) were aliquoted into a single well of a 96-well tissue culture plate (USA Scientific ...
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No products found
because this supplier's products are not listed.
Joana T. Lima, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 5μL of Lipofectamin 2000 and 0.6μg of HaloTag9-LaminB1 plasmid were diluted separated and incubated in OPTIMEM (Alfagene) for 30 min ...
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No products found
because this supplier's products are not listed.
Yanniv Dorone, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Seeds obtained from the T0 parental lines were sown on MS medium supplemented with 30 mg/L Kanamycin (G-Biosciences) for selection of successfully transformed transgenics ...
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No products found
because this supplier's products are not listed.
Genevieve. McCluskey, et al.,
bioRxiv - Immunology 2021
Quote:
... in the presence or absence of FH (1.5-100nM) was added to 4g/L (11.8μM) plasminogen-depleted fibrinogen (Enzyme Research Laboratories, UK) in 10mM HEPES ...
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No products found
because this supplier's products are not listed.
Neil J Rzechorzek, et al.,
bioRxiv - Biochemistry 2020
Quote:
... HEK293-F cells were grown in 400 mL batches in 2 L non-baffled Erlenmeyer flasks (TriForest Enterprises Inc, #FPC2000S) to a cell density of ∼1×106 cells/mL.
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No products found
because this supplier's products are not listed.
Barr Tivon, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 4 µl of 20 mM alkyne-peptide in DMSO were mixed with 10 µl of 5 mM BDP-TMR-azide (Lumiprobe). To the mixture was added 50 µl water ...
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No products found
because this supplier's products are not listed.
Takaya Ogasawara, et al.,
bioRxiv - Neuroscience 2021
Quote:
... A key difference in success was due to the use of glass coated electrodes (Alpha Omega) in Monkey S versus thinner epoxy electrodes in Monkey L (FHC). Recording locations ...
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No products found
because this supplier's products are not listed.
Jillian M. DiMuzio, et al.,
bioRxiv - Immunology 2021
Quote:
A homogeneous time-resolved fluorescence (hTRF) assay [26] comprised of terbium-labeled anti-human IgG (H+L) (Cisbio, custom label) donor and AF488-labeled anti-HIS (Cell Signaling ...
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No products found
because this supplier's products are not listed.
Francesco De Virgiliis, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 100 µl of lysate (corresponding to 80 mg of proteins) were analysed with the Multi-Neurotrophin Rapid Screening ELISA kit (Biosensis), according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Michael S. Haney, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The dissociated neurons were then plated onto 24-well plates with coverslips coated with poly-l-lysine (Newcomer Supply, 1339A) using minimum essential medium (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Ling He, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the cells were lysed in 250 µl cell lysis buffer supplemented with protease inhibitors and subjected to a pull-down assay for activated Rac1 (#BK035, Cytoskeleton). Briefly ...
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No products found
because this supplier's products are not listed.
Jared O. Kroll, et al.,
bioRxiv - Biochemistry 2023
Quote:
... streptavidin enrichment and tryptic digestion were performed as previously described.58 Peptides (25 μL) were transferred to 200 μL volume AQ brand silanized glass vial inserts in 2 mL glass autosampler vials (MicroSolv) and stored at −20 °C.
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No products found
because this supplier's products are not listed.
Vineet Choudhary, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cells containing plasmids were grown in synthetic complete (SC) media containing 6.7 g/l yeast nitrogen base without amino acids (United States Biologicals), an amino acid mix (United States Biologicals) ...
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No products found
because this supplier's products are not listed.
Drake M. Mellott, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Recombinant proteases were obtained from the following vendors: human cathepsin L (Millipore Sigma, Athens Research and Technology, Inc., (Texas A&M) or R&D Systems (UCSD) ...
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No products found
because this supplier's products are not listed.
Maureen C. Onyeziri, et al.,
bioRxiv - Microbiology 2021
Quote:
... 5 µl of 20 mg/ml suspensions of 20 nm colloidal gold conjugated WGA or 10 nm gold conjugated PNA (EY Laboratories) were added to the suspension and incubated at room temperature for 20 min ...
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No products found
because this supplier's products are not listed.
Eric Esposito, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The RNA was further purified by twice mixing the aqueous supernatant with 700 μL of acidic phenol chloroform and centrifuging the solution in a 5Prime Phase Lock Gel Heavy 2 ml tube (Andwin Scientific) at 16,000 rcf to separate the phases ...
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No products found
Anthony G. Pacholko, Lane K. Bekar,
bioRxiv - Neuroscience 2019
Quote:
Fecal pellets were collected from the colon during sacrifice and immediately stored on dry ice for subsequent ethanol extraction (100 μL ethanol/10 mg fecal powder) and equal volume measurement of corticosterone metabolites using an ELISA-based Assay Kit (Arbor Assays). Fecal samples were chosen over serum as they better represent a long-term average less influenced by rapid stress-induced changes in corticosterone expression ...
-
No products found
because this supplier's products are not listed.
Wenhe Lin, et al.,
bioRxiv - Genomics 2023
Quote:
... We measured plasma oxidized LDL (oxLDL) concentrations (U/L) immunologically using a sandwich-style enzyme-linked immunoabsorbent assay (Mercodia Oxidized LDL ELISA; ALPCO Diagnostics). Lipoprotein size distributions were estimated as absorbance in large size particles minus absorbance in small size particles ...
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No products found
because this supplier's products are not listed.
Marisol Romero-Tejeda, et al.,
bioRxiv - Cell Biology 2023
Quote:
... consisting of RPMI 1640 supplemented with 2 mg/mL fatty acid-free bovine serum albumin (GenDEPOT, A0100), 200 µg/mL L-ascorbic acid 2-phosphate (Wako, 321-44823) and 2 µM Wnt-C59 (Biorbyt, orb181132). Medium was then changed on day 4 and then every other day with RBAI consisting of RPMI 1640 supplemented with 500 µg/mL fatty acid-free bovine serum albumin ...
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No products found
because this supplier's products are not listed.
Jennifer Reck, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 50 μl 2 M hydrochloric acid and 50 μl of 5% trifluoroacetic acid were added and peptides collected using BioPureSPN Mini C18 spin columns (Part #HUMS18V from The Nest Group) and analyzed with an Orbitrap Elite Mass Spectrometer (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
Zhifeng Zeng, et al.,
bioRxiv - Microbiology 2021
Quote:
... This was followed by an incubation with secondary antibody Dylight 488 Affinipure Goat anti-Rabbit IgG (H+L)(1:1000 dilution)(Abbkine, Wuhan, China) in the PBST buffer containing 2% BSA at room temperature for 2 h ...
-
No products found
because this supplier's products are not listed.
Kristi Dietert, et al.,
bioRxiv - Neuroscience 2022
Quote:
... A total of 21,600 cells were plated onto the upper wells (n=6/condition) of 96-well poly-l-ornithine (PLO) coated chemotaxis chambers (10 μm pore size, Neuro Probe Inc) and maintained in DMEM containing l-glutamine (1 mM) ...
-
No products found
because this supplier's products are not listed.
Sarah Wazir, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 0.5 μL per spot of the reaction solution was transferred to the nitrocellulose membrane using Echo 650 acoustic liquid dispenser (Labcyte, Sunnyvate, CA). After drying of the spots ...
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No products found
because this supplier's products are not listed.
Kristen R. Breit, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... using 200 µl HPLC grade acetonitrile containing 10 ng/mL 11-nor-9-Carboxy-Δ9-THC-D9 as an internal standard (Cerilliant T-007-1ML). The supernatant was centrifuged for 10 min (4°C ...
-
No products found
because this supplier's products are not listed.
Motohiro Nonaka, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... with human 15 N-terminal ANXA1 residues plus a cysteine residue at 16 position (MAMVSEFLKQAWFIEC) and L-MC16 mutants were synthesized by Bio-Synthesis (Lewisville, TX). IsodTIT7 ...
-
No products found
because this supplier's products are not listed.
Jackline Abu-Nassar, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Plants were sprayed using a chain-driven sprayer delivering 300 L ha-1 with a flat-fan 8001E nozzle (TeeJet®, Spraying Systems Co., Wheaton, IL, USA). Experiments were conducted at Newe-Ya’ar Research Center in a net-house during the natural season for A ...
-
No products found
because this supplier's products are not listed.
Amandine Maurin, et al.,
bioRxiv - Evolutionary Biology 2024
Quote:
... filled with 500µL of LB inoculated with 2µL of an overnight culture of SeeT2 with a 96-pin replicator (#140500, Boekel Scientific; Feasterville, PA, USA). Overnight co-culture was performed at 37°C within an incubator with ventilation (Aqua-lytic ...
-
No products found
because this supplier's products are not listed.
Rick G. Kim, et al.,
bioRxiv - Plant Biology 2024
Quote:
... a 20 μL mixture of 14N- and 15N-labeled samples was loaded onto a Tris-glycine 4-20% polyacrylamide gel (Mini-PROTEAN TGX; Bio-Rad) and the proteins of the sample separated by electrophoresis at 60 V for 1 h ...
-
No products found
because this supplier's products are not listed.
Raeann Goering, et al.,
bioRxiv - Molecular Biology 2022
Quote:
C2bbe1 cells (CRL-2102) were maintained in DMEM with 110 mg/L Sodium Pyruvate (Thermo 11995065) supplemented with 10% FBS (Atlas Biologicals F-0500-D), penicillin-streptomycin (Thermo 15140122 ...
-
No products found
because this supplier's products are not listed.
Iñigo Landa, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... after which cells were spun down and resuspended in Coon’s modified F12 medium with penicillin/streptomycin/L-glutamine (P/S/G; Gemini; #400-110) and 0.5% bovine brain extract (BBE, Hammond Cell Tech, cat. #2007-NZ), plated into CellBind plates (Corning Inc. ...
-
No products found
because this supplier's products are not listed.
Bridget A. Luckie, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... 30 mg/L 1-butanol as an internal standard for 15 minutes at 3000 rpm in a Vortex Genie 2 (Scientific Industries, INC, NY, USA). After centrifugation at 14,500 × g ...
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No products found
because this supplier's products are not listed.
Anna Tasegian, et al.,
bioRxiv - Physiology 2024
Quote:
... a 50% conditioned media (1:1 dilution in advanced D-MEM/F12 base media) was generated from genetically modified L-WRN mouse fibroblast cell line (ATCC, #CLR-3276™, ATCC, LGC Standards, Middlesex, UK) cultured in advanced D-MEM/F12 (Thermofisher ...
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