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No products found
because this supplier's products are not listed.
Satyaki Sengupta, et al.,
bioRxiv - Cancer Biology 2021
Quote:
The pLKO.1 shRNA construct targeting MYCN (TRCN0000020694) was purchased from Sigma-Aldrich and the pLKO.1 GFP shRNA was a gift from D ...
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No products found
because this supplier's products are not listed.
Carolyn Jablonowski, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... MYCN (Santa Cruz Biotechnology, 53993, Mouse antibody) ...
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No products found
because this supplier's products are not listed.
Ingrid M. Saldana-Guerrero, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... The chr17q1q-MYCN hESC line was generated by introducing a TetOn-PiggyBac plasmid (PB-TRE3G-MYCN, plasmid#104542, Addgene) carrying the wild-type version of the MYCN gene100 via nucleofection using the Lonza 4D-Nucleofector System as per the manufacturer’s instructions (Amaxa 4D-Nucleofector Basic Protocol for Human Stem Cells) ...
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No products found
because this supplier's products are not listed.
Raúl Rabadán, et al.,
bioRxiv - Cancer Biology 2020
Quote:
We used an Adamts12 Mouse shRNA Plasmid (OriGene, Locus ID: 239337) and transfected LLC/2-luc-M38 (Caliper ...
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No products found
because this supplier's products are not listed.
Hamed Alborzinia, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and incubated for 15-40 min before adding MYCN-amplified IMR5/75 cells stably expressing a doxycycline-regulable MYCN shRNA (2,100 cells/well) using a Multidrop dispenser (Thermo Fisher Scientific). Two treatment conditions were screened in triplicate ...
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No products found
because this supplier's products are not listed.
Clare Rogerson, et al.,
bioRxiv - Cell Biology 2019
Quote:
... shRNA constructs were obtained from QIAGEN (SureSilencing Akt1 shRNA plasmids) or Origene (HuSH Myh9 shRNA constructs).
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No products found
because this supplier's products are not listed.
Rohtem Aviram, et al.,
bioRxiv - Cell Biology 2024
Quote:
... MISSION® shRNA lentiviral plasmids (Merck) were used for knockdown of LOX (shLOX1 – TRCN0000045991 ...
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No products found
because this supplier's products are not listed.
Shreya Budhiraja, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... shRNA plasmids were procured from Genecopoeia (Rockville, MD, USA), and overexpression plasmids were obtained from AddGene (Watertown ...
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No products found
because this supplier's products are not listed.
Kai Voeltzke, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... MYCN (1:1,000, Cell Signaling #9405), GAPDH (1:1,000 ...
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No products found
because this supplier's products are not listed.
Lisa Baker, et al.,
bioRxiv - Neuroscience 2020
Quote:
... except cells were transduced with AAV5 containing a control scrambled shRNA plasmid or a FL2 shRNA plasmid (Vector Biolabs). shRNA sequences were cloned into a D2-U6 vector ...
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No products found
because this supplier's products are not listed.
Adam G Grieve, et al.,
bioRxiv - Biochemistry 2020
Quote:
... in 35mm plates with 0.5 μg of pLKO shRNA or pLVX expression plasmids (Takara), 0.35 μg pCMV-dR8.2 and 0.15 μg pCMV-VSVG ...
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No products found
because this supplier's products are not listed.
Natalia G. Sampaio, et al.,
bioRxiv - Immunology 2021
Quote:
... and 1 μg shRNA plasmid using Fugene 6 (Promega). The next day ...
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No products found
because this supplier's products are not listed.
Martin Michaelis, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... MYCN (abcam, Cambridge, UK), and survivin (R&D Systems ...
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No products found
because this supplier's products are not listed.
Zuen Ren, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Mouse GPx2 plasmid (GenScript) was subcloned by PCR into Xho1/BamH1 restriction sites of lentiviral expression vector pLVX-puro (Clontech) ...
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No products found
Md Imamul Islam, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Scramble (LV-shRNA-scramble, #LVP015-G) and Trx1 shRNA plasmids (LV-shRNA-Trx1, #488660940395) were purchased from Applied Biological Materials Inc. ...
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No products found
because this supplier's products are not listed.
Shiyi Wang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... a DNA fragment containing hU6 and shRNA was amplified from pLKO.1-shRNA using Phusion High-Fidelity DNA Polymerase (NEB) with primers that introduced SpeI restriction sites (Forward Primer ...
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No products found
because this supplier's products are not listed.
Nerea Berastegui, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... two different short hairpin RNAs (shRNAs) and a scramble shRNA were cloned into pSIH1-H1-copGFP shRNA lentiviral vector (System Biosciences #SI501A-A). Generation of lentiviruses and further specifications are described in supplemental methods.
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No products found
because this supplier's products are not listed.
Adriana Mañas, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... MYCN (1:200, Proteintech), TH (1:1000 ...
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No products found
because this supplier's products are not listed.
Valerio Taverniti, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... 38HK were transiently transfected with lentiviral shRNA plasmids using TransIT-Keratinocytes Transfection Reagent (Mirus) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Xizi Chen, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Lentivirus expressing short-hairpin RNAs was prepared by transfecting PLKO.1 shRNA plasmids and packaging plasmids containing psPAX2 and pMD2.G into 293T cells using PEI (Polysciences). Collected conditional media containing virus particles were used to transduce cells in the growth media supplemented with Polybrene for 24 hours ...
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No products found
because this supplier's products are not listed.
Ratnadeep Mukherjee, et al.,
bioRxiv - Immunology 2020
Quote:
... 5 µg of NOSIP shRNA plasmids or control TRC2 plasmid was mixed with 100 µl Cell Line Nuceleofector Solution V (Lonza) and added to one million cells ...
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No products found
because this supplier's products are not listed.
Sanghee Yun, et al.,
bioRxiv - Animal Behavior and Cognition 2023
Quote:
... Each annealed oligonucleotide was ligated into the adeno-associated virus (AAV2) plasmid (pAAV-EGFP-shRNA; Stratagene, La Jolla, CA)(Hommel et al. ...
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No products found
because this supplier's products are not listed.
Teha Kim, et al.,
bioRxiv - Immunology 2023
Quote:
... or the mouse kappa light chain plasmid (InVivoGen). The gBlock DNA and plasmids were cut with the specified enzymes ...
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No products found
because this supplier's products are not listed.
Christine Chiasson-MacKenzie, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The shRNA constructs targeting mouse ezrin (5’-ATTTCCTTGTTATAATCTCCG-3’) in a pLKO-puro.1 vector from GE Healthcare and described in27 ...
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No products found
because this supplier's products are not listed.
Mary E. Herndon, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... double-stranded oligonucleotides encoding short hairpin RNAs (shRNAs) targeting the mouse E-cadherin mRNA were annealed and then cloned into the pSIREN-RetroQ retroviral vector (BD Biosciences; Franklin Lakes, NJ). The shRNA targeting sequence for E-cadherin was 5’-TACATCCTTCATGTGAGAGTG-3’ ...
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No products found
because this supplier's products are not listed.
Tim van Groningen, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and MYCN (Novus Biologicals, 23960002, 1:300). Images were acquired using an Olympus BX63 microscope and DP80 camera along with the CellSense Dimension imaging software.
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No products found
because this supplier's products are not listed.
Ulli Heydasch, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Transfection of plasmids for knockdown purposes (shRNA-vectors) was performed using FuGene®HD (Roche Diagnostics) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Francesca Puca, et al.,
bioRxiv - Cancer Biology 2020
Quote:
The inducible shRNA was obtained from Cellecta by cloning into a pRSIT16-U6Tet-sh-CMV-TetRep-2A-TagRFP-2A-Puro vector the following hairpin sequence:
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No products found
because this supplier's products are not listed.
Laura Grunewald, et al.,
bioRxiv - Immunology 2024
Quote:
... on day 0 with induced/uninduced 5×106 SK-N-AS-TR-MYCN tumor cells in 50µl Matrigel™ (Corning)/50µl phosphate-buffered saline ...
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No products found
because this supplier's products are not listed.
Gonzalo Quiroga-Artigas, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Newly synthesized shRNAs were then purified using the Direct-zol™ RNA MiniPrep Kit (Zymo Research), eluted in 35μl of nuclease-free H2O and quantified with a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific) ...
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No products found
because this supplier's products are not listed.
Liliane Khoryati, et al.,
bioRxiv - Immunology 2020
Quote:
... mouse CD25-expressing plasmid (Sino Biological) was transiently transfected into HEK 293T/17 adherent cells using PEI 25K (ratio 3:1 ...
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No products found
because this supplier's products are not listed.
KO McNerney, et al.,
bioRxiv - Cancer Biology 2021
Quote:
Homozygous TH-MYCN mice were randomized to receive intraperitoneal injections of 100 μg of anti-GD2 antibody (14G2a; BioLegend), 100 μg of a murine isotype-matched antibody to control for Fc receptor binding (murine IgG2a/κ ...
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No products found
because this supplier's products are not listed.
D. Czernecki, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... plasmids were isolated with NucleoSpin Plasmid QuickPure kit (Macherey-Nagel) and suspended in water ...
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No products found
because this supplier's products are not listed.
Raphael Vidal, et al.,
bioRxiv - Molecular Biology 2023
Quote:
SH-EP-MYCN-ER cells were plated in 384-well plates (PerkinElmer), treated with Dox and/or 4-OHT and fixed with methanol for 20 min ...
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No products found
because this supplier's products are not listed.
Yuhao Shi, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using LipoD293™ Transfection Reagent (SignaGen Laboratories.) Conditioned media containing virions was harvested after 24 and 48 hours ...
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No products found
because this supplier's products are not listed.
Joachim M. Surm, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
... shRNAs were synthesized with AmpliScribe T7-Flash Transcription Kit (Epicentre, Charlotte, NC) for 15 h followed by 15 min treatment with 1 µl of DNase I ...
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No products found
because this supplier's products are not listed.
Min Hao, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... the plasmid was extracted using plasmid minipreparation Kit (TIANGEN, #DP103), respectively ...
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No products found
because this supplier's products are not listed.
S. M. Nayeemul Bari, et al.,
bioRxiv - Microbiology 2021
Quote:
... Confirmed plasmids were purified using EZNA Plasmid Mini Kit (Omega Bio-tek), and where indicated ...
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No products found
because this supplier's products are not listed.
Joshua M Corbin, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... 22Rv1 cells expressing dox-inducible shNT or shUGUUUGC shRNAs were mixed with 50% basement membrane extract (R&D Systems, Minneapolis, MN, USA) and injected subcutaneously into the flanks (3×106 cell per injection) ...
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No products found
because this supplier's products are not listed.
Vedavathi Madhu, et al.,
bioRxiv - Cell Biology 2024
Quote:
... For mouse primary antibody Mouse on Mouse Kit (Vector laboratories, BMK2202) was used for blocking and primary antibody incubation ...
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No products found
because this supplier's products are not listed.
Joanna Tannous, et al.,
bioRxiv - Microbiology 2022
Quote:
... and the plasmid DNA was isolated using Quantum Prep®Plasmid Midiprep Kit (Biorad) according to the manufacturer’s’ instructions ...
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No products found
because this supplier's products are not listed.
Alessio D. Nahmad, et al.,
bioRxiv - Immunology 2020
Quote:
... or anti-mouse IgG or anti-mouse IgG1 or anti-mouse IgM (Jackson ImmunoResearch) at 2μg/ml in PBST and were incubated for two hours ...
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No products found
because this supplier's products are not listed.
Thale Kristin Olsen, et al.,
bioRxiv - Cancer Biology 2021
Quote:
ChIP- and combined ChIP/RNA-seq analysis of TH-MYCN tumor samples were performed by Active Motif. For enhancer analyses ...
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No products found
because this supplier's products are not listed.
Blair K. Schneider, et al.,
bioRxiv - Genetics 2022
Quote:
... UAS-shRNAs were inserted into the attP site at 86F (BL #24749) by BestGene. gRNA flies for CRISPRi were generated using the protocol from CRIPR fly design (66–72) ...
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No products found
because this supplier's products are not listed.
Damián Hernández, et al.,
bioRxiv - Neuroscience 2020
Quote:
... LIN28 and shRNA against p53 [28] in feeder- and serum-free conditions using TeSR-E7 medium (Stem Cell Technologies). Subsequently ...
-
No products found
because this supplier's products are not listed.
Alexandra M. Amen, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... DV:3.5mm) of 6-7 week old female athymic nu/nu mice (inducible shRNA TMZ combination experiment: The Jackson Laboratory, RRID:IMSR_JAX:002019 ...
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No products found
because this supplier's products are not listed.
Kathryn A. Jacobs, et al.,
bioRxiv - Cell Biology 2019
Quote:
... HRP-conjugated secondary antibodies (anti-rabbit, mouse Ig, mouse IgG1, mouse IgG2a, and mouse IgG2b) were purchased from Southern Biotech. Alexa-conjugated secondary antibodies were from Life Technologies.
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No products found
because this supplier's products are not listed.
Miriam R. Fein, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... mouse (Miltenyi Biotec) at 4°C for 5 min ...
-
No products found
because this supplier's products are not listed.
Sumit Sen Santara, et al.,
bioRxiv - Immunology 2021
Quote:
... NK cells (0.2 mg/mouse of rabbit anti–mouse NK1.1, BioXCell) or 300 μg/per mouse anti-CSF1R (BioXCell ...
-
No products found
because this supplier's products are not listed.
Wenpeng Liu, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Anti-mouse (LI-COR) secondary antibody diluted 1:10,000 was used and membranes were imaged via Odyssey imaging system ...