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No products found
because this supplier's products are not listed.
Ying Zhu, et al.,
bioRxiv - Systems Biology 2022
Quote:
... anti-TIMM23 (1:1000, mouse, DB Biotech, 611222). After washing 3 times with TBST ...
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No products found
because this supplier's products are not listed.
Luis E. Martinetti, et al.,
bioRxiv - Neuroscience 2021
Quote:
... we used a virus-retrobead or saline-retrobead mixture (red RetroBeads, Lumafluor, Cat# R180). When comparing different AAV serotypes ...
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No products found
because this supplier's products are not listed.
Cecilia Martinez Campos, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Cells were split and infected with NL4-3 virus for 48h and then pulsed with 100µM of either 4SU (Carbosynth Cat #NT06186) or 6SG (Sigma Cat #858412 ...
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No products found
because this supplier's products are not listed.
Isadora Matias, et al.,
bioRxiv - Neuroscience 2021
Quote:
Protein concentration in cell extracts was measured by the BCA Protein Assay Kit (Cole-Parmer). Forty micrograms protein/lane was electrophoretically separated on a 10% SDS polyacrylamide gel and electrically transferred onto a Hybond-P PVDF transfer membrane (Millipore ...
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No products found
because this supplier's products are not listed.
Chun-Che Tseng, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Instant-Bands Protein Stain (EZBiolab, Parsippany, NJ) were used to visualize input and eluted material on a Typhoon FLA 7000 (GE Healthcare ...
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No products found
because this supplier's products are not listed.
Sandro Roier, et al.,
bioRxiv - Immunology 2023
Quote:
... The protein vaccines were alum-adjuvanted by adsorption of the recombinant fusion proteins to aluminum hydroxide (Alhydrogel®; Croda, Frederikssund, Denmark) as previously described22 ...
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No products found
because this supplier's products are not listed.
Tim Vangansewinkel, et al.,
bioRxiv - Neuroscience 2022
Quote:
... A mouse intrathecal catheter (Alzet®, DURECT Corp.) was carefully inserted via this opening into the intrathecal space at the midline ...
-
No products found
because this supplier's products are not listed.
Qianyan Li, et al.,
bioRxiv - Genetics 2022
Quote:
... and the column was washed with lysis buffer to remove unbound proteins before eluting the proteins with 50 μM biotin (Chem-Impex Int’l) in low salt buffer (20mM HEPES-KOH pH 7.2 ...
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No products found
because this supplier's products are not listed.
Steven Swendeman, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Activated Protein C was obtained from Enzyme Research laboratories. For initial studies ...
-
No products found
because this supplier's products are not listed.
Beatriz Gil, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Recombinant alpha- synuclein protein was a gift from rPeptide (GA, USA).
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No products found
because this supplier's products are not listed.
Abishek Chandrashekar, et al.,
bioRxiv - Microbiology 2022
Quote:
... or B.1.1.529 (Omicron; GISAID ID: EPI_ISL_7358094.2) Spike proteins (21st Century Biochemicals). 106 peripheral blood mononuclear cells well were re-suspended in 100 µL of R10 media supplemented with CD49d monoclonal antibody (1 µg/mL ...
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No products found
because this supplier's products are not listed.
Mizuho Nosaka, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... tPA (Mouse tPA ELISA Kit, PA92, Oxford Biomedical Research, Rochester Hills, MI), uPA (Active mouse uPA Functional Assay Kit ...
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Lenti/Retro Virus
Cat# LVB1000,
Conservation Buffer (1mL), USD $95.00/mL
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Andrew S. Flies, et al.,
bioRxiv - Immunology 2020
Quote:
... Digested proteins in PBS were diluted 1:1 in Squalvax (Oz Biosciences # SQ0010) to a final concentration of 0.1 μg/μL and was mixed using interlocked syringes to form an emulsion ...
-
No products found
because this supplier's products are not listed.
Daniele Merico, et al.,
bioRxiv - Genetics 2019
Quote:
... PVDF membrane was cut at 75 kDa according to protein ladder (BlueElf, FroggaBio). The higher molecular weight portion of the membrane was incubated with ATP7B antibody (Abcam ...
-
No products found
because this supplier's products are not listed.
Daisuke Shimura, et al.,
bioRxiv - Cell Biology 2021
Quote:
... using Mouse Mitochondrial DNA Copy Number Assay kit (Detroit R&D, Detroit, MI) for the samples from the mouse heart or Human Mitochondrial DNA Monitoring Primer Set (TaKaRa Bio ...
-
No products found
because this supplier's products are not listed.
Juan Yang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... P7 - P75 mouse brains were coronal sectioned using Compresstome (Precisionary Instruments LLC, NC) to a thickness of 30 - 50 μm.
-
No products found
because this supplier's products are not listed.
Carina C D Joe, et al.,
bioRxiv - Bioengineering 2021
Quote:
Residual host-cell protein (HCP) was quantified using the HEK293 HCP ELISA kit (Cygnus Technologies) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Hagit Hak, et al.,
bioRxiv - Plant Biology 2024
Quote:
... and images of protein bands were acquired and quantified using the Alliance Q9 software (UVITEC).
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No products found
because this supplier's products are not listed.
Nicole Stantial, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... or anti-Top2 (TopoGen, #TG2014) antibody ...
-
No products found
because this supplier's products are not listed.
Sarah K. Williams Avram, et al.,
bioRxiv - Neuroscience 2019
Quote:
... All cages were maintained on high-density ventilated racks (Super Mouse 750, Lab Products Inc.). Mice were maintained on a 12-h light cycle (lights off at 1500h ...
-
No products found
because this supplier's products are not listed.
Y Vicioso, et al.,
bioRxiv - Immunology 2021
Quote:
... and proteins were crosslinked to DNA in two steps: (1) by adding DSG (Disuccinimidyl glutarate; ProteoChem) to a final concentration of 2mM for 45 minutes at room temperature followed by washing once with PBS and (2 ...
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No products found
because this supplier's products are not listed.
Nils H. Rustmeier, et al.,
bioRxiv - Biochemistry 2023
Quote:
... proteins were incubated with oligosaccharides (Forssman antigen trisaccharide: Elicityl, France; Blood group A trisaccharide: Biosynth, UK) for 5 minutes prior to their addition to the cellular samples (∼1×106 cells in 100 µL) ...
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No products found
because this supplier's products are not listed.
Amanda L. Peiffer, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Active protein was quantified by titrating in the known active site protease inhibitor FPR-CMK (Haematologic Technologies). To determine the KM the initial fluorescence data ...
-
No products found
because this supplier's products are not listed.
Pooja Kesari, et al.,
bioRxiv - Biophysics 2021
Quote:
... PEG 8000) with 0.4 µl protein + 0.4 µl reservoir as sitting drops in 96-well XJR trays (Rigaku Reagents) at 14°C ...
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No products found
because this supplier's products are not listed.
Santhosh Gatreddi, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The precipitated protein was pelleted by centrifugation and the L-lactate concentration was measured using the D-/L-lactate assay kit (Megazyme Inc.), as previously described.3 To examine the reversibility of inactivation ...
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No products found
because this supplier's products are not listed.
Marie O. Pohl, et al.,
bioRxiv - Microbiology 2021
Quote:
... A mouse (#Ab00458-1.1) or rabbit (Ab00458-23.0) anti-dsRNA antibody (9D5; Lucerna-Chem) was used to stain for SARS-CoV-2 infected cells ...
-
No products found
because this supplier's products are not listed.
Dara Bree, et al.,
bioRxiv - Neuroscience 2019
Quote:
... a 96-well plate was coated with a mouse anti-CGRP capture antibody (Bertin Bioreagent) and incubated overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Kristina D. Micheva, et al.,
bioRxiv - Neuroscience 2023
Quote:
... A 10 nm gold-labeled goat anti-mouse IgG secondary Ab (SPI Supplies, West Chester, PA) was used at 1:25 for 1 hr ...
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No products found
because this supplier's products are not listed.
José Gustavo Ramírez-Paredez, et al.,
bioRxiv - Microbiology 2020
Quote:
Nucleic acids were used for the detection of tilapia lake virus and nodavirus by quantitative PCR using the commercial kits: Path-TiLV-EASY and Path-Betanodavirus-EASY (Primerdesign, Southampton, UK) in the platform Genesig q16® (Primerdesign ...
-
No products found
because this supplier's products are not listed.
Alisa Fox, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were washed and incubated in Accutase cell detachment solution for 10 min at room temperature to gently remove any surface-bound virus while preserving epitopes for flow cytometry analysis (Innovative Cell Technologies; as described in (39)) ...
-
No products found
because this supplier's products are not listed.
Eric Waltari, et al.,
bioRxiv - Immunology 2019
Quote:
... Blocking solution (sciBLOCK Protein D1M solution, Scienion) was added at 200 μL/well with a multichannel pipet and allowed to incubate without agitation for 1 hour ...
-
No products found
because this supplier's products are not listed.
Cassian Afting, et al.,
bioRxiv - Bioengineering 2024
Quote:
WNT surrogate-Fc Fusion Protein (Wnt-surrogate; ImmunoPrecise Antibodies Ltd. ...
-
No products found
because this supplier's products are not listed.
Eli Fritz McDonald, et al.,
bioRxiv - Cell Biology 2021
Quote:
... purified from B lymphocyte hybridoma cells using a Recombinant Protein G - Sepharose 4B Affinity Column on an ÄKTA start protein purification system (GE Life Science Product # 29022094). Antibodies were used in immunoblotting buffer (5% bovine serum albumin (BSA ...
-
No products found
because this supplier's products are not listed.
Justin Judd, Jonathan Lovas, Guo N. Huang,
bioRxiv - Developmental Biology 2019
Quote:
... Proteins were then transferred to PVDF-Plus membranes (Spectrum Chemical) and blocked in 5% milk in TBST (0.1% Tween 20) ...
-
No products found
because this supplier's products are not listed.
Sofia Rossini, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... while recombinant human IDO1 protein was obtained by Giotto Biotech. Construct expressing murine Src was obtained from Origene.
-
No products found
because this supplier's products are not listed.
Christopher Jonkergouw, et al.,
bioRxiv - Biochemistry 2023
Quote:
... dosed with LPS (50µl/mouse) using a P100 pipette (Gilson). The animals were held upright for a short period of time after dosing to allow for substance distribution down the respiratory tract ...
-
No products found
because this supplier's products are not listed.
Malachy Guzman, et al.,
bioRxiv - Genetics 2023
Quote:
... Each mouse was weighed with a analytical laboratory scale (Ohaus) immediately prior to recording ...
-
No products found
because this supplier's products are not listed.
Nicholai M. Hensley, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... Varying volumes of concentrated protein solution and luciferin assay mix (Targeting Systems; prepared to manufacturer’s specifications but with unknown concentration for mammalian cells ...
-
No products found
because this supplier's products are not listed.
Marvin J. Sandoval, et al.,
bioRxiv - Immunology 2021
Quote:
... and then incubated with mouse IFNλ2/3 DNA probes (Advanced Cell Technologies), followed by a series of RNA Scope adaptor probes ...
-
No products found
because this supplier's products are not listed.
Karen M. Dunkerley, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Protein phosphorylation was monitored to completion using Phos-Tag AAL™ (NARD Institute Ltd) SDS–PAGE ...
-
No products found
because this supplier's products are not listed.
Swayam Prakash Srivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Urine albumin levels were assayed using a Mouse Albumin ELISA Kit (Exocell, Philadelphia, PA).
-
No products found
because this supplier's products are not listed.
Catherine S. Millar-Haskell, et al.,
bioRxiv - Bioengineering 2022
Quote:
ECM proteins were covalently attached to PAA using the crosslinker Sulfo-SANPAH (Covachem; 13414-100). To begin ...
-
No products found
because this supplier's products are not listed.
Liangxi Wang, et al.,
bioRxiv - Genomics 2022
Quote:
... Cells were then treated with 10 ng/mL recombinant mouse TNFα (Cell Applications, cat# RP2031-20) for 45 min in basal Endothelial Cell Growth Media MV2 (PromoCell ...
-
No products found
because this supplier's products are not listed.
Weigao Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 300 μL pure protein solution was added to a 1 mm pathlength quartz cuvette (Starna Cells, USA). The scanning wavelength starts from 300 nm and ends at 190 nm ...
-
No products found
because this supplier's products are not listed.
Tsunaki Hongu, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... mice were intravenously injected with clodronate-liposome (100-150 μl per mouse, every other day, Liposoma BV). Short-term experiments were performed by treatment with clodronate-liposome on day 14 ...
-
No products found
because this supplier's products are not listed.
Kimberley El, et al.,
bioRxiv - Cell Biology 2023
Quote:
... levels were normalized to total protein levels (Sigma-Aldrich, #E3138, 1:1000 with Nacalai USA Signal Enhancer HIKARI), and total (i.e ...
-
No products found
because this supplier's products are not listed.
Xue-Wei Wang, et al.,
bioRxiv - Neuroscience 2019
Quote:
... the right optic nerve of the mouse was exposed intraorbitally and crushed with Dumont #5 fine forceps (Fine Science Tools) for 5 s at approximately 1 mm behind the optic disc ...
-
No products found
because this supplier's products are not listed.
Marina Johnson, et al.,
bioRxiv - Immunology 2020
Quote:
Samples were screened for IgG to SARS-CoV-2 N protein using a commercially available kit (Epitope Diagnostics Inc, San Diego, USA) as previously described (8).
-
No products found
because this supplier's products are not listed.
Kim S. Friedmann, et al.,
bioRxiv - Immunology 2020
Quote:
... APC-labelled anti-MART-1 (ELAGIGILTV) dextramers (Immudex, 1:5), APC-labelled A*0201 dextramer negative control (Immudex ...
-
No products found
because this supplier's products are not listed.
Anna Voleníková, et al.,
bioRxiv - Genetics 2023
Quote:
... using 4′,6-diamidino-2-phenolindole (DAPI) (1.5 μg/mL in anti-fade; Cambio, Cambridge, UK) counterstaining ...