-
No products found
because this supplier's products are not listed.
Samuel Finkel, et al.,
bioRxiv - Bioengineering 2023
Quote:
... tissues were incubated at 37°C and 5% O2 for 90 min in Tyrode’s solution containing 5 μM calcium indicator Cal 520 AM (21130, AAT Bioquest) and 0.025% Pluronic F127 (P2443 ...
-
No products found
because this supplier's products are not listed.
Fabio Tommasini, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Slides were then dehydrated in 5-minute ethanol baths of ascending percentages (70%, 90%, 100%) followed by Histoclear (Cat. # HS-200, National Diagnostics, USA) before being mounted with DPX mounting medium (Cat ...
-
No products found
because this supplier's products are not listed.
Linghao Hu, et al.,
bioRxiv - Bioengineering 2022
Quote:
... bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl) ethyl sulfide (BPTES, 10 μm, ASTATECH, A11656) was added to the cells in CM3 1 hour before imaging (35) ...
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No products found
because this supplier's products are not listed.
Isabell Ramming, et al.,
bioRxiv - Microbiology 2023
Quote:
... was coated with 10 μg/mL of a capture antibody in 50 μL Phosphate Buffered Saline (PBS) (13C4, hybridoma cell line from American Type Culture Collection, Manassas ...
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No products found
because this supplier's products are not listed.
Yevgen Zolotarov, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and 2 mM L-glutamine at 37 °C in a humidified incubator with 5% (v/v) CO2 (New Brunswick Galaxy 170s). Cells were seeded 6 hours before transient transfection with Lipofectamine 2,000 (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Benjamin Lin, Jonathan Luo, Ruth Lehmann,
bioRxiv - Cell Biology 2021
Quote:
... blocked for 1 hour in 2% (v/v) BSA (Bioworld, 40220068-1) in TBST (TBS with .3% Tween-20 ...
-
No products found
because this supplier's products are not listed.
Burt M Sharp, Qin Jiang, Panjun Kim, Hao Chen,
bioRxiv - Neuroscience 2023
Quote:
... 2-(3-(4-chloro-3-fluorophenyl)-5-ethyl-1H-1,2,4-triazol-1-yl)-N-(3,5-di-chlorobenzyl)acetamide (MR-L2) was from Targetmol Chemicals ...
-
No products found
because this supplier's products are not listed.
Ragna S. Häussler, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Activation buffer consisting of 10 mg/ml 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (C1100, ProteoChem) and 10 mg/ml Sulfo-N-hydroxysulfosuccinimide (Sulfo-NHS ...
-
No products found
because this supplier's products are not listed.
Rebecca A. Callahan, et al.,
bioRxiv - Neuroscience 2019
Quote:
... 100 Hz pulse trains (1 ms pulse width) at 2-5 V (A-M systems Isolated Pulse Stimulator Model 2100). Confocal imaging was performed as described above ...
-
No products found
because this supplier's products are not listed.
Hanna Fokt, et al.,
bioRxiv - Microbiology 2023
Quote:
... All strains were grown at 37 °C under anaerobic conditions (gas mixture: 5% H2, 5% CO2, 90% N2) in a vinyl anaerobic chamber (Coy Lab Products).
-
No products found
because this supplier's products are not listed.
Binbin Yi, et al.,
bioRxiv - Microbiology 2023
Quote:
Cells were fixed with 4% (w/v) paraformaldehyde (PFA) in PBS (pH 8.0) for 15 min and incubated with 5% (v/v) goat serum (Cedarlane, Burlington, ON, Canada), in PBS (pH 8.0 ...
-
No products found
because this supplier's products are not listed.
Emily C. Erickson, et al.,
bioRxiv - Cancer Biology 2022
Quote:
Membranes were blocked in 5% (w/v) nonfat dry milk (Fisher Cat# NC9022655/190915ASC) or 5% (w/v) bovine serum albumin (Boston Bioproducts Cat. # P-753) in Tris-buffered saline (TBS ...
-
No products found
because this supplier's products are not listed.
Saja Gharaba, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Then Blocking solution 5% w/v BSA (Chem-Impex, USA) in TBST was added to the cells for 1 hour ...
-
No products found
because this supplier's products are not listed.
Alexandros Sfikas, et al.,
bioRxiv - Cell Biology 2019
Quote:
... siRNA 2 5’ GCCCUAUCCCUUUACGUCA (Eurogentec). Dharmacon ONTarget plus SMARTpool ...
-
No products found
because this supplier's products are not listed.
E. Thynne, et al.,
bioRxiv - Plant Biology 2024
Quote:
... Luminescence was captured over 2 hours (90 cycles) using a PHERAstar FS plate reader (BMG Labtech) controlled through the PHERAstar control software ...
-
No products found
because this supplier's products are not listed.
Eugene Serebryany, et al.,
bioRxiv - Biophysics 2022
Quote:
... and IPTG (99%) from Omega Scientific (Tarzana, CA).
-
2 Well Chambered Cover Glass with #1.5 high performance cover glass (0.170±0.005mm), with lid,...
Cat# C2-1.5H-N,
48/case, $219.00
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Laura R Lee, et al.,
bioRxiv - Plant Biology 2023
Quote:
... 5 mL of 1/2 MS with 2% low melt agarose was cast into imaging cuvettes (CellVis product number #C1-1.5H-N) after being filtered through a 0.45 micron nylon filter to remove any particulates that might disturb the path of the light sheet to prepare media “blankets” ...
-
No products found
because this supplier's products are not listed.
KJ Weaver, RA Holt, E Henry, SD Pletcher,
bioRxiv - Physiology 2022
Quote:
... was dissolved in 80% (v/v) ethanol at 10 mM concentration and marked by blue dye (5% [w/v] FD&C Blue No. 1; Spectrum Chemical) and stored at −20°C ...
-
No products found
because this supplier's products are not listed.
Cesare Granata, et al.,
bioRxiv - Cell Biology 2023
Quote:
Metabolite extraction was carried out as previously described (Bachem et al., 2019). Briefly ...
-
No products found
because this supplier's products are not listed.
Anna M. Pfaller, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 2 µl of AAV9-GFAP(0.7)-mNR3C1-2A-EGFP particles (5 × 1013 GC/ml; Vector Biolabs, AAV-266053) or PBS (sham control ...
-
No products found
because this supplier's products are not listed.
Caleb R. Carr, et al.,
bioRxiv - Microbiology 2024
Quote:
... 2 µL of 10 µM 5’ PacBio round 2 forward primer (PacBio_5pri_RND2), 2 µL of 10 µM 3’ PacBio round 2 reverse primer (PacBio_3pri_RND2) ...
-
No products found
because this supplier's products are not listed.
Wayne Mitchell, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 140 mM NaCl) and stained at room temperature with 1 µg/ml DAPI and 5 µl of Annexin V FITC (Tonbo Biosciences, San Diego, CA) for 15 minutes in the dark ...
-
No products found
because this supplier's products are not listed.
Jonathan R. Soucy, et al.,
bioRxiv - Bioengineering 2020
Quote:
... The collected spinal cords were minced into ~2 mm pieces and transferred to a 0.2% (v/v) trypsin solution in Hibernate®-A without Ca2+ (BrainBits, LLC) at 37 °C to dissolve the neural tissue ...
-
No products found
because this supplier's products are not listed.
Sven Klumpe, et al.,
bioRxiv - Biophysics 2021
Quote:
... 5 ug/ml Insulin (Cell Applications), 10 mM HEPES (pH 7.4) ...
-
No products found
because this supplier's products are not listed.
Madalyn M. Won, et al.,
bioRxiv - Microbiology 2023
Quote:
... Chromatography for peptide separation was performed using increasing organic proportion of acetonitrile (5 – 40 % (v/v)) over a 120 min gradient) on a self-packed analytical column using PicoTipTM emitter (New Objective, Woburn, MA) using Reprosil Gold 120 C-18 ...
-
No products found
because this supplier's products are not listed.
Houjian Zhang, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 5-ethynyl-2’-deoxyuridine (EdU) (APExBIO, Cat. No. B8337) was injected intraperitoneally at a concentration of 50 mg/kg body weight and mice were euthanized by cervical dislocation 12 or 24 h later.
-
No products found
because this supplier's products are not listed.
Ádám Györkei, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... the membranes were incubated with 5% (w/v) milk powder-TBST including anti-GFP (Chromotek) as primary antibody (diluted to1:1000 ...
-
No products found
because this supplier's products are not listed.
Timna C. Bergmann, et al.,
bioRxiv - Cell Biology 2023
Quote:
... absorbance at 450 nm was measured immediately with the Infinite Mono 200 Platereader (Tecan, Männedorf, Switzerland) and experiments were analysed with the provided Excel based freeware ELISA analysis tool v1.0 (Raybiotech ...
-
No products found
because this supplier's products are not listed.
James S Martenson, et al.,
bioRxiv - Cell Biology 2020
Quote:
... followed by spheroplasting for 90 min at 30°C in zymolyase buffer containing 0.43 mg/mL zymolyase 20T (Amsbio 120491-1). The resulting spheroplasts were washed once with 30°C zymolyase buffer ...
-
No products found
because this supplier's products are not listed.
Pedro A. Avila-Lopez, et al.,
bioRxiv - Genomics 2024
Quote:
... and 5 ml of penicillin/streptomycin solution (ScienCell). The FHC cells were grown in DMEM:F12 Medium (ATCC ...
-
No products found
because this supplier's products are not listed.
Juan Yang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Sulfo-Cyanine 3 Azide (2-5 uM final, Lumiprobe, #D1330), and fresh Sodium Ascorbate (100 mM final ...
-
No products found
because this supplier's products are not listed.
Matthew D. J. Dicks, et al.,
bioRxiv - Bioengineering 2022
Quote:
... and sample Ad-DogTag:DogCatcher-RBD were applied to a 1/2 Cu-mesh C-flat grid that had been plasma-cleaned for 10 s using a 25% v/v O2 / 75% v/v Ar mixture in a Solarus 950 Plasma Cleaner (Gatan). Grids were manually plunge frozen in liquid ethane ...
-
No products found
because this supplier's products are not listed.
Shristi Baral, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Borosilicate glass pipettes (Sutter Instrument BF100-58-10, 2-5 MΩ) were pulled using a laser micropipette puller (Sutter Instrument Model P-2000) ...
-
No products found
because this supplier's products are not listed.
Kohgaku Eguchi, Ryuichi Shigemoto,
bioRxiv - Neuroscience 2022
Quote:
... GIRK3: chicken anti-GST IgY (5 µg/ml, Bethyl Laboratories) + rabbit anti-GIRK3 IgG (XX µg/ml ...
-
No products found
because this supplier's products are not listed.
James T. Grist, et al.,
bioRxiv - Physiology 2022
Quote:
... 90uL of Ethyl pyruvate was polarized for 45 minutes in a prototype polarizer (Alpha System, Oxford Instruments) prior to dissolution with 4.5mL of sodium hydroxide as previously described 38 ...
-
No products found
because this supplier's products are not listed.
Fiona Whelan, et al.,
bioRxiv - Microbiology 2020
Quote:
... 0.02% (w/v) 5 μm diameter silica beads (Bangs Labs), 8 pM Alexa Fluor 488 (AF488)-labelled Sgo_R2-8S666C/S1086C protein and ...
-
No products found
because this supplier's products are not listed.
Lili Qin, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... DCs were collected and cocultured with autologous CD8+ T cells with the ratio between 1:4 to 1:10 in AIM-V medium with 5% autologous serum and 30 ng/ml IL-21 (Cellgenix, Germany). Two days later ...
-
No products found
because this supplier's products are not listed.
Grace I. Borlee, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 100 nM of 2-chloro-adenosine-5’-O-monophsphate (2-Cl-5’-AMP, Axxora, LLC) for internal standardization ...
-
No products found
because this supplier's products are not listed.
Charles Bayly-Jones, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 5 μL of diluted C9-depleted serum (Complement Tech; diluted 1 in 5 with 1×DGHB; 2.5% (w/v) D-glucose ...
-
No products found
because this supplier's products are not listed.
Maria G. Noval, et al.,
bioRxiv - Microbiology 2020
Quote:
... 90-95% confluent T175 flask (Thomas Scientific) of Vero E6 (1×107 cells ...
-
No products found
because this supplier's products are not listed.
Júlia T. Oliveira, et al.,
bioRxiv - Neuroscience 2021
Quote:
... transferred to 2.0 mL pre-filled tubes with 1.5 mm Triple-Pure™ Zirconium homogenizer beads (Benchmark Scientific, USA) containing a fresh amount of Lysis Buffer (ThermoFisher Scientific ...
-
No products found
because this supplier's products are not listed.
Anthony Brown, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The cells were then harvested in 300 µL freezing 80% acetonitrile (v/v) into 1.5 mL tubes and lysed by Bullet Blender (Next Advance) at 4 °C followed by centrifugation at 21,000 x g for 5 min at 4 °C ...
-
No products found
because this supplier's products are not listed.
Manish Kuchakulla, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Code: AAH-CYT-5-2 (RayBiotech, Norcross, GA, USA) according to the manufacturer’s instructions ...
-
No products found
Siling Liu, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The production of lentiviral particles up to 109 IU/ml was purified by Ultra-Pure Lentivirus purification kits (Applied Biological Materials Inc. Vancouver). AAV preparation ...
-
No products found
because this supplier's products are not listed.
Fugui Niu, et al.,
bioRxiv - Neuroscience 2021
Quote:
... for 1 hr and laminin (Trevigen, 5 μg/ml) overnight at 37°C ...
-
No products found
because this supplier's products are not listed.
Evan F. Fullerton, et al.,
bioRxiv - Neuroscience 2022
Quote:
DAMGO-stimulated [35S]GTPγS binding to PAG membrane protein was assessed by incubating membrane protein (100ug) in the presence or absence of [35S]GTPγS (0.1nM) (specific activity 1250 Ci/mmol, American Radiolabeled Chemicals, Missouri) and various concentrations of DAMGO (2 to 30,000 nM ...
-
No products found
because this supplier's products are not listed.
Marieta Caganova, et al.,
bioRxiv - Immunology 2022
Quote:
... DG75 cells with 5 μg/ml anti-human IgM (DIANOVA) for 3 min ...
-
No products found
because this supplier's products are not listed.
Emil Sporre, et al.,
bioRxiv - Systems Biology 2023
Quote:
Thermal unfolding of proteins was measured in absence and presence of metabolites by nano differential scanning fluorimetry (F350/F330) using a Prometheus NT.48 (NanoTemper) at 95% excitation power over a temperature gradient from 20 °C to 95 °C at an increase of 1 °C per minute ...
-
No products found
because this supplier's products are not listed.
Yuzuki Shimamori, et al.,
bioRxiv - Microbiology 2020
Quote:
... Samples were negatively stained with 2% phosphotungstic acid (w/v, pH 7.0) and observed using a JEM-1200EXII electron microscope (JEOL, Japan). Micrographs were taken at an accelerating voltage of 80 kV.
-
No products found
because this supplier's products are not listed.
David O. Dias, et al.,
bioRxiv - Neuroscience 2020
Quote:
... The spinal cord was then fully crushed for 2 seconds with Dumont No.5 forceps (11295-00, Fine Science Tools) without spacers and that had been filed to a width of 0.1 mm for the last 5 mm of the tips16,42 ...