-
No products found
because this supplier's products are not listed.
Peter H. Yoon, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and 2 volumes of RNA binding buffer (ZYMO) was added to each volume of RNA sample ...
-
No products found
because this supplier's products are not listed.
Braulio Cardenas-Benitez, et al.,
bioRxiv - Biophysics 2023
Quote:
... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...
-
No products found
because this supplier's products are not listed.
Sam C. Nalle, et al.,
bioRxiv - Immunology 2020
Quote:
HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...
-
No products found
because this supplier's products are not listed.
Esther A. Mozipo, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Recombinant human bone morphogenetic protein-2 (BMP-2) and the human BMP-2 DuoSet enzyme-linked immunosorbent assay (ELISA ...
-
No products found
because this supplier's products are not listed.
Harold Marcotte, et al.,
bioRxiv - Immunology 2021
Quote:
... The SARS-CoV-2 S2 N defined peptide pool contains 41 synthetic peptides binding to human HLA derived from the S and N proteins of the SARS-CoV-2 virus (#3620-1, Mabtech AB)58.
-
No products found
because this supplier's products are not listed.
Viola Nähse, et al.,
bioRxiv - Cell Biology 2022
Quote:
Nucleotide binding assays were carried out by measuring the incorporation of 2,3-0-N-Methyl-anthraniloyl (Mant)-labelled nucleotides (Jena Bioscience) using an Synergy2 plate reader (BioTek Instruments Inc. ...
-
No products found
because this supplier's products are not listed.
Masaki Tsuchiya, et al.,
bioRxiv - Cell Biology 2022
Quote:
... [2-[(Methylthio)(2H-pyrrol-2-ylidene)methyl]-1H-pyrrole](difluoroborane) (TCI, M2609) was reacted with dibenzocyclooctyne-amine (TCI ...
-
No products found
because this supplier's products are not listed.
Benjamin N. Bell, et al.,
bioRxiv - Immunology 2021
Quote:
His-tagged SARS-CoV-2 RBD (300 nM) was loaded on His1K biosensors (Pall ForteBio) to a load threshold of 0.4-0.6 nm ...
-
No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
-
No products found
because this supplier's products are not listed.
Ranmal A. Samarasinghe, et al.,
bioRxiv - Neuroscience 2021
Quote:
... rabbit anti-MECP2 (Diagenode C15410052), 1:1000 ...
-
No products found
because this supplier's products are not listed.
Ge Song, et al.,
bioRxiv - Immunology 2020
Quote:
... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
-
No products found
because this supplier's products are not listed.
Tarlan Mamedov, et al.,
bioRxiv - Bioengineering 2020
Quote:
... commercially available recombinant Spike Protein of SARS-CoV-2 (RBD, His Tag, Arg319-Phe541, MM~ 25 kDa, MBS2563882, MyBioSource, USA) was added and incubated for 2 h at 37 °C ...
-
No products found
because this supplier's products are not listed.
Miao-Hsi Hsieh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 S1 protein containing a C-terminal His-tag (Acro; S1N-C52H3) (5 μg/ml) was tagged with anti-His antibody (Genetex; GT395) (1:100 ...
-
No products found
because this supplier's products are not listed.
Yuki Takamatsu, et al.,
bioRxiv - Microbiology 2019
Quote:
... HEK293 cells (150 cm2 dish) were transfected with 6 μg of each protein-coding plasmid by using TransIT (Mirus). Cell lysis and elution were performed as described above ...
-
No products found
because this supplier's products are not listed.
Matthew J. Burke, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
-
No products found
because this supplier's products are not listed.
Paul R. Dominguez-Gutierrez, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (34) ...
-
No products found
because this supplier's products are not listed.
Ana F. Ferreira, et al.,
bioRxiv - Neuroscience 2023
Quote:
Human samples: qPCR experiments were conducted using the SensiFAST Probe Hi-ROX Master Mix (Bioline) and the TaqMan Gene Expression Assays (Applied Biosystems ...
-
No products found
because this supplier's products are not listed.
Pille Hallast, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries using 1.5 M human cells as input were generated with Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) following the manufacturer’s protocol with the following modification ...
-
No products found
because this supplier's products are not listed.
Efil Bayam, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 2% glucose and the appropriate –His dropout mix (CSM-His, MP Biomedicals) or in autophagy induction medium SD-N ...
-
No products found
because this supplier's products are not listed.
Gabriele Liuzzi, Antonello Mallamaci,
bioRxiv - Developmental Biology 2023
Quote:
- αMecP2 (rat polyclonal IgG2a serotype, Active Motif #61291), 3 μg/reaction.
-
No products found
because this supplier's products are not listed.
Nishant Mehta, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Binding of VISTA was detected indirectly by first adding 1:750 rabbit anti-6-HIS (Bethyl, A190-114F) and then adding 1:7500 anti-rabbit-HRP (Novus Biologicals ...
-
No products found
because this supplier's products are not listed.
Anushka Nayak, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and tri-methyl lysine (Abclonal). Anti-GAPDH-HRP was used to measure even loading.
-
No products found
because this supplier's products are not listed.
Marin Boutonnet, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 2-methyl-6-(phenylethynyl)-pyridine hydrochloride (MPEP, HelloBio®); human angiotensin II (HelloBio®) ...
-
No products found
because this supplier's products are not listed.
Kaihuai Li, et al.,
bioRxiv - Microbiology 2020
Quote:
... HtsH2C-HA-His or HtsH3C-Myc-His proteins (100 ng) were incubated with CIAP (Solarbio, Beijing, China) at 28°C for 60 min ...
-
No products found
because this supplier's products are not listed.
Katarzyna Bogucka-Janczi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... human recombinant GST-fusion ERK3 protein (SignalChem) and E.coli purified ARP3 ...
-
No products found
because this supplier's products are not listed.
Shivathmihai Nagappan, Kevin M. Franks,
bioRxiv - Neuroscience 2021
Quote:
... and methyl tiglate (Alfa Aesar, A11964).
-
No products found
because this supplier's products are not listed.
Chengbao Ma, et al.,
bioRxiv - Microbiology 2023
Quote:
Protein binding kinetics was analyzed using BLI assays with the Octet RED96 instrument (Molecular Devices) following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Ryan J. Garrigues, et al.,
bioRxiv - Biochemistry 2022
Quote:
... and 2% normal human serum (NHS; Innovative Research) in GHB++ (10 mM HEPES (pH 7.3) ...
-
No products found
because this supplier's products are not listed.
Alexander W. Justin, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Green fluorescent protein (GFP) and Red Fluorescent Protein (RFP) human umbilical vein endothelial cells (HUVECs, Promocell), normal human lung fibroblasts (NHLFs ...
-
No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... and His-tagged SARS-CoV-2 S1 RBD protein lacking the S1/S2 boundary furin site (EpiGentek) were added at a concentration of 10 ng/well to the Ni-NTA-coated strips and incubated for 1h at 37°C ...
-
No products found
because this supplier's products are not listed.
Silvio Schmidt, et al.,
bioRxiv - Neuroscience 2020
Quote:
... the S100 calcium-binding protein B (S100B; rabbit, Synaptic system), and BrdU (mice ...
-
No products found
because this supplier's products are not listed.
Xi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or plastic-bottom (Ibidi: DLD1, HEK293) 96-well plates at densities targeting 60-70% confluence at the time of transfection ...
-
No products found
because this supplier's products are not listed.
Mayowa M Amosu, et al.,
bioRxiv - Immunology 2023
Quote:
... received intranasal CpG and 300 μg anti-PD-1 antibody (BioXCell, BE0146-A0) in 200 μL of PBS via intraperitoneal (IP ...
-
No products found
because this supplier's products are not listed.
Uju Joy Okaa, et al.,
bioRxiv - Microbiology 2021
Quote:
... Transcription factor binding was read at 450 nm using a Synergy 2 microplate reader (BioTek, US) and expressed as fold-change relative to the uninfected (UI ...
-
No products found
because this supplier's products are not listed.
Jennifer L. Reedy, et al.,
bioRxiv - Immunology 2022
Quote:
... Dectin-2(murine)-IgG1 Fc (human) fusion protein (ENZO Life Sciences, Inc, Farmingdale, NY) or IgG1 Fc (human ...
-
No products found
because this supplier's products are not listed.
Robert W. P. Glowacki, et al.,
bioRxiv - Microbiology 2019
Quote:
... Protein purification was accomplished using the pETite N-His vector (Lucigen). PCR primers were designed to amplify products for BT2807 and BT2808 containing all amino acids downstream of the predicted signal peptide sequences ...
-
No products found
because this supplier's products are not listed.
MEENAKSHI TETORYA, et al.,
bioRxiv - Plant Biology 2023
Quote:
... The His-tagged peptides (GMA4CG_WT and GMA4CG_V6) phospholipid binding assays were performed using the PIP StripsTM (Echelon Biosciences Inc., CA) as previously described (Islam et al. ...
-
No products found
because this supplier's products are not listed.
M Iqbal Hossain, et al.,
bioRxiv - Neuroscience 2023
Quote:
... or Human IgG F(ab)2 (Rockland, USA) overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Nayab Fatima, et al.,
bioRxiv - Neuroscience 2020
Quote:
Primary human astrocytes (passage 2-7) obtained from ScienCell were cultured in astrocyte medium (ScienCell ...
-
No products found
because this supplier's products are not listed.
Eliana Nachman, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... after seeding and imaged regularly with a Leica DM IL LED system equipped with a HI PLAN I Phase 2 40x/0.50 (Leica) objective lens.
-
No products found
because this supplier's products are not listed.
Alberto Domingo López-Muñoz, et al.,
bioRxiv - Microbiology 2021
Quote:
... and human anti-SARS-CoV-2 N mAb (N18) followed by IRDye® 680RD Goat anti-Human IgG Secondary Ab (LI-COR # 926-68078).
-
No products found
because this supplier's products are not listed.
Hoa Quynh Do, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and lipid-protein nanodiscs (MSP1D1-His-POPC, MSP1D1-His-DMPG, MSP1D1-His-DMPC, Cube Biotech) were used to solubilize in-situ synthesized PCFT ...
-
No products found
because this supplier's products are not listed.
Jiechao Zhou, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and purified human C1q protein (2 µg/mL, Complement Technology) was suspended in GVB++ Buffer (Complement Technology ...
-
No products found
because this supplier's products are not listed.
Fransky Hantelys, et al.,
bioRxiv - Cell Biology 2019
Quote:
Binding analyses were performed with recombinant protein VASH1 (Abnova H00022846-P01) at 100 µg/ml over the immobilized FGF1 ...
-
No products found
because this supplier's products are not listed.
Noémie Aurine, et al.,
bioRxiv - Cell Biology 2019
Quote:
... or universal IFN-type (a recombinant human IFN-alpha protein hybrid; Pbl Assay Science, 11200-2). Bat cells seeded in 12-well plates at 80% confluence were treated for 6 h at 37°C with either poly IC (0–1000 ng/mL ...
-
No products found
because this supplier's products are not listed.
Marina Boudigou, et al.,
bioRxiv - Immunology 2021
Quote:
... recombinant human Interleukin-2 (rh IL-2) (20 ng/mL, ImmunoTools) subsequently referred to as primed stimulation ...
-
No products found
because this supplier's products are not listed.
Chen Su, Yuxin Wang, Xiaoke Yang, Junyu Xiao,
bioRxiv - Biochemistry 2023
Quote:
... The bound proteins were eluted with the binding buffer supplemented with 10 mM desthiobiotin (IBA Lifesciences). The samples were analyzed by SDS-PAGE and detected by immunoblotting using antibodies to the strep tag (1:3,000 ...
-
No products found
because this supplier's products are not listed.
Yunqing Yu, et al.,
bioRxiv - Plant Biology 2024
Quote:
Methyl Methacrylate/Butyl Methacrylate (Electron Microscopy Sciences, Cat ...
-
No products found
because this supplier's products are not listed.
Steven J. Kunnen, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... in INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT ...
-
No products found
because this supplier's products are not listed.
Miljana Tanić, et al.,
bioRxiv - Genomics 2021
Quote:
The Ovation RRBS Methyl-Seq System 1–16 (NuGen, now Tecan) user manual was followed to generate sequencing libraries by enzymatically digesting 100 ng of gDNA using MspI ...