-
No products found
because this supplier's products are not listed.
Tao Jing, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Viruses were generated by co-transfecting 107 cells plated the previous day in 15 cm dishes with 30 µg of total plasmid DNA (pNLX.Luc.R-.ΔAvrII and a vesicular stomatitis virus G envelope expressor at 6:1 ratio using PolyJet™ DNA transfection reagent; SignaGen Laboratories). Two days post-transfection ...
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No products found
because this supplier's products are not listed.
Jamie C. Moffa, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was removed from the upper chamber of the concentrator and stored in low protein binding tubes (Eppendorf #022431064) at -80°C.
-
No products found
because this supplier's products are not listed.
Victoria L. Corbit, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Virus was injected using a syringe pump (Harvard Apparatus) fitted with a syringe (Hamilton ...
-
No products found
because this supplier's products are not listed.
Yaping Ma, et al.,
bioRxiv - Physiology 2022
Quote:
... Serum E2 levels were measured with a mouse/rat E2 kit from Calbiotech (El Cajon, CA). The sensitivity for E2 was 3 pg/mL ...
-
No products found
because this supplier's products are not listed.
Jean-Philippe Guégan, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... E2 UbcH5c (2.5 µM, Enzo Life Sciences), biotinylated Ubiquitin (2.5 µM ...
-
No products found
because this supplier's products are not listed.
Sarah Cherkaoui, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... Antigen retrieval was performed with E2 (Leica Microsystems) retrieval solution for 20min ...
-
No products found
because this supplier's products are not listed.
Zhongyan Lu, et al.,
bioRxiv - Immunology 2021
Quote:
... IE2 and envelope glycoprotein B (Mabtech, OH) at a final concentration of 1 mg/ml for each individual peptide (Supplemental Table 2) ...
-
No products found
because this supplier's products are not listed.
Tianze Xu, et al.,
bioRxiv - Cell Biology 2022
Quote:
... E2 (3 μg/kg every other day, Solarbio, Beijing, China), or E2+DFP (80 mg/kg daily ...
-
No products found
because this supplier's products are not listed.
Ellen Van Gulck, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 48h post transfection virus was collected and concentrated with PEG Virus Precipitation Kit (BioVision Inc, USA) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Rebecca S. Brown, et al.,
bioRxiv - Microbiology 2020
Quote:
... E2 signal was imaged and quantified using the Odyssey Fc Imaging System (LI-COR Biosciences) to determine particle number ...
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No products found
because this supplier's products are not listed.
Maria A. Cruz, et al.,
bioRxiv - Cell Biology 2022
Quote:
... envelope (pMD2.G) and packaging (psPAX2) plasmids were amplified in Escherichia coli (GCI-L3, GeneCopoeia) and silica column purified (Qiagen Maxiprep ...
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No products found
because this supplier's products are not listed.
Takanobu Tagawa, et al.,
bioRxiv - Microbiology 2022
Quote:
... The virus pellet was resuspended to EBM2 (Lonza) media ...
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No products found
because this supplier's products are not listed.
Camilla Schinner, et al.,
bioRxiv - Cell Biology 2021
Quote:
... virus particle containing supernatant was collected and enriched using LentiConcentrator (OriGene). Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Linling He, et al.,
bioRxiv - Microbiology 2019
Quote:
The kinetics of E2 cores and nanoparticle binding to HCV-specific antibodies was measured using an Octet Red96 instrument (fortéBio, Pall Life Sciences). All assays were performed with agitation set to 1000 rpm in fortéBio 1× kinetic buffer ...
-
No products found
because this supplier's products are not listed.
Yuanzhong Zhang, et al.,
bioRxiv - Biophysics 2023
Quote:
... SARS-CoV-2 envelope protein antibody (ProSci; 9169; 1:1000), SARS-CoV-2 Nucleocapsid protein (RayBiotech ...
-
No products found
because this supplier's products are not listed.
Nia Toshkova, et al.,
bioRxiv - Immunology 2023
Quote:
... measles virus mosaic hemagglutinin (viral proteins were obtained from Immune Technology, New York, NY). Plates were incubated with proteins usually diluted to 5 μg/ml in PBS ...
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No products found
because this supplier's products are not listed.
Filippo Bianchini, et al.,
bioRxiv - Immunology 2022
Quote:
... Avi-tagged SARS-CoV-2 RBD and SD1-RBD (both corresponding to SARS-CoV-2 ancestral virus) were biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity). Ovalbumin (Sigma ...
-
No products found
because this supplier's products are not listed.
Jingyi Guo Fuglstad, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Fluorescent signal from the virus was amplified by immunostaining against Red Fluorescent Protein (RFP) (catalog no.5F8, Chromotek GmbH, Germany), followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no ...
-
No products found
because this supplier's products are not listed.
Jenny Y. Pham, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
This study used Escherichia virus T4 (American Type Culture Collection [ATCC] #11303-B4) and three wild-type bacterial hosts ...
-
No products found
because this supplier's products are not listed.
Sanjib Saha, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Protein expression was normalized to total protein (DM-TP01, Protein Simple) and compared between cell types.
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No products found
because this supplier's products are not listed.
Marion Thépaut, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins were detected using InstantBlue protein stain (Expedeon) according to the supplier’s instructions.
-
No products found
because this supplier's products are not listed.
Shigekuni Okumura, et al.,
bioRxiv - Physiology 2023
Quote:
... Prostaglandin E2 (PGE2) and indomethacin were obtained from Calbiochem (Billerica, MA, USA) and Tokyo Chemical Industry (Tokyo ...
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No products found
because this supplier's products are not listed.
Xiaozhen Liu, et al.,
bioRxiv - Genetics 2022
Quote:
... and vesicular stomatitis virus G protein (VSV-G) plasmid using polyetherimide (PEI) (B600070, ProteinTech Group, Chicago, USA) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Maria Rojec, et al.,
bioRxiv - Systems Biology 2019
Quote:
... Cell envelopes were disrupted using a Bioruptor Plus sonication system (Diagenode s.a., Belgium) for 10 cycles ...
-
No products found
because this supplier's products are not listed.
Joshua J. Sims, et al.,
bioRxiv - Genetics 2021
Quote:
... We measured reporter virus transduction activity on a luminometer (BioTek) using the Renilla Glo Kit (Promega ...
-
No products found
because this supplier's products are not listed.
Shih-Chia Yeh, et al.,
bioRxiv - Zoology 2022
Quote:
... and stained using 1:400 mouse monoclonal anti-envelope antibody (4G2) and 1:20,000 secondary anti-mouse Dylight 680 (Rockland). Focus forming units (ffu ...
-
No products found
because this supplier's products are not listed.
Qixiang He, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The virus titers were measured by flow cytometry assay (Expression Systems).
-
No products found
because this supplier's products are not listed.
Anthony J. Snyder, Andrew T. Abad, Pranav Danthi,
bioRxiv - Microbiology 2021
Quote:
... the virus-resistant cell population was transferred to fresh 150-mm dishes (Greiner Bio-One) and allowed to expand in growth medium supplemented with 2 μg/ml puromycin (InvivoGen ...
-
No products found
because this supplier's products are not listed.
Enakshi Roy, Wen Shi, Bin Duan, St Patrick Reid,
bioRxiv - Microbiology 2020
Quote:
... Mock or virus-infected cells were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, USA) for 30 min and permeabilized with 0.1% Triton X (Fisher Bioreagents ...
-
No products found
because this supplier's products are not listed.
Paula Lagan, et al.,
bioRxiv - Microbiology 2023
Quote:
... Successful virus isolation was confirmed either by haemagglutination of 0.5% chicken blood cells (Envigo #S.B-0008) or by RRT-PCR.
-
No products found
because this supplier's products are not listed.
Katarzyna Bogucka-Janczi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cdc42 and ARP3 proteins or ARP2/3 protein complex (Cytoskeleton) were used as bait and full-length GST-ERK3 (SignalChem ...
-
No products found
because this supplier's products are not listed.
Stefanie Krug, et al.,
bioRxiv - Immunology 2021
Quote:
... Protein concentrations were determined by CB-X protein assay (G-Biosciences) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
May Daher, et al.,
bioRxiv - Immunology 2020
Quote:
... Cas9 protein (PNA bio) and gRNA were incubated at room temperature for 15 min in a 3:1 reaction with 15μg Cas9 and 5000 ng gRNA (2500ng of sgRNA#1 and 2500ng of sgRNA#2).47 The incubation product (gRNA and Cas9 complex ...
-
No products found
because this supplier's products are not listed.
Qiuli Shi, et al.,
bioRxiv - Microbiology 2021
Quote:
Proteins were labeled using the Monolith NT Protein Labeling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol and using a buffer consisting of 20 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Ana Lucia Rosales Rosas, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Primary antibody anti-E2 protein [Chk265] (Absolute Antibody, Wilton, UK) was diluted in PBS-T (1:500 ...
-
No products found
because this supplier's products are not listed.
Dina Hany, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... 60-day release E2 pellets (Innovative Research of America) were implanted subcutaneously into the upper left flank ...
-
Recombinant Antigen
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Ramesh Kumar, et al.,
bioRxiv - Cell Biology 2023
Quote:
... CHIKV E2 (1:3000, The Native antigen company MAB12130-200), and EGFP (1:3000 ...
-
No products found
because this supplier's products are not listed.
Gabrielle L. Turvey, et al.,
bioRxiv - Cell Biology 2023
Quote:
... At 48 h post-transfection the supernatant containing virus was harvested and filtered through a low-protein binding filter (0.45µm, Sarstedt) to remove HEK debris ...
-
No products found
because this supplier's products are not listed.
Clara Young, et al.,
bioRxiv - Immunology 2024
Quote:
... Biotinylated E2 (described above) or biotin-SP (long spacer) ChromPure human IgG (Jackson Immunoresearch) were mixed with PE-Klickmers according to manufacturer’s instructions (HCV rE2 ...
-
No products found
because this supplier's products are not listed.
Qiulong Yan, et al.,
bioRxiv - Microbiology 2020
Quote:
The DNA and RNA of virus were extracted by using TIANamp Virus DNA / RNA Kit (TIANGEN) according to the manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Morgan E. Hernandez Scudder, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and estradiol (E2) in single samples (due to larger serum volume needed for this assay) using radioimmunoassays (Testosterone: MP Biomedicals #07189102 ...
-
No products found
Yang Li, et al.,
bioRxiv - Immunology 2021
Quote:
PAOC cells were immortalized with Lenti-hTERT virus (ABM; cat# G200) following manufacturer’s instructions and clonal sorting/expansion ...
-
No products found
because this supplier's products are not listed.
Christopher M. Hoel, et al.,
bioRxiv - Biophysics 2022
Quote:
... and P1 virus was generated from cells transfected with FuGENE transfection reagent (Active Motif) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Helene Sertznig, et al.,
bioRxiv - Microbiology 2021
Quote:
... The virus containing supernatant was then purified by filtration through 0.30 μm MACS SmartStrainers (Miltenyi Biotec), aliquoted and stored at -80 °C ...
-
No products found
because this supplier's products are not listed.
Chenyan Ma, et al.,
bioRxiv - Neuroscience 2023
Quote:
... starting at day 3 post virus injection (250 mg tamoxifen per kg of chow, Research Diets). Mice were gavaged with two additional doses of tamoxifen (250 mg/kg body weight ...
-
No products found
because this supplier's products are not listed.
Wei-Siang Liau, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Protein identification was carried out using Protein Pilot 5.0 (SCIEX) for database searching.
-
No products found
because this supplier's products are not listed.
Lei Zhang, José M Jiménez-Gómez,
bioRxiv - Plant Biology 2019
Quote:
... Total protein was extracted using PEB protein extraction buffer (Agrisera Antibodies) according to the manufacturer‘s instructions ...
-
No products found
because this supplier's products are not listed.
Pablo Castro-Córdova, et al.,
bioRxiv - Microbiology 2020
Quote:
... and protein concentration was quantified by BCA protein kit (RayBiotech USA). Next ...
-
No products found
because this supplier's products are not listed.
Hui Chen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The proteins were first purified using CaptivA Protein A affinity resin (Repligen), unless otherwise specified ...
-
No products found
because this supplier's products are not listed.
David Gonzalez-Perez, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Proteins were separated by SDS-PAGE (12% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and transferred to PVDF membranes using an iBlot2 Gel Transfer Device (Thermo Fisher Scientific) ...