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No products found
because this supplier's products are not listed.
Stephanie Braun-Galleani, et al.,
bioRxiv - Genetics 2019
Quote:
... The plate was incubated for 4 hours at 30 °C to allow asci to swell and become more visible under the 40X lens of a SporePlay+ dissection microscope (Singer Instruments). Whole asci were picked up and placed individually on the YPD plate ...
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No products found
because this supplier's products are not listed.
Elizabeth A. DeRiso, et al.,
bioRxiv - Immunology 2019
Quote:
... For live cell studies samples were maintained at 37°C by establishing a stable temperature gradient using an air blower (Nevtek, Williamsville, VA) and a lens heater (Bioptechs, Butler, PA).
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No products found
because this supplier's products are not listed.
Robert V. Blair, et al.,
bioRxiv - Pathology 2020
Quote:
Serum samples collected at preinfection and at necropsy were tested for binding IgG antibodies against SARS-CoV-2 S1/S2 proteins using an ELISA kit from XpressBio (cat# SP864C). The assays were performed per directions of the manufacturer ...
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No products found
because this supplier's products are not listed.
Sung-Eun Choi, et al.,
bioRxiv - Pathology 2020
Quote:
... Total protein in the hydrolysed sample was also measured using the Total Protein Assay Kit (QuickZyme) and the relative amount of collagen per protein was analysed.
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No products found
because this supplier's products are not listed.
Ayan Rajgarhia, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... and protein extraction was carried out using a total protein extraction Kit (BioChain Institute, Inc. Hayward, CA). Protein concentration was evaluated using the Modified Lowry Protein Assay (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Donna Ye, et al.,
bioRxiv - Microbiology 2019
Quote:
... Media supplemented with milk protein (Hardy Diagnostics) was made by first sterilizing 2x media stocks (TSB or R2B ...
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No products found
because this supplier's products are not listed.
Paresh P Kulkarni, et al.,
bioRxiv - Cell Biology 2023
Quote:
Protein C activity in WT and Apoh-/- plasma was performed using the Chromogenix Coamatic Protein C activity kit (Diapharma). Briefly ...
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No products found
because this supplier's products are not listed.
Koichi Sato, Aiko G.M. Hendrikx, Puck Knipscheer,
bioRxiv - Biochemistry 2023
Quote:
... The wild-type protein was overexpressed as a N-terminal His6-tagged protein in the E.coli JM109(DE3) cells (Intact Genomics) cultured in 4.4 L LB medium and purified by the previously described method53 ...
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No products found
because this supplier's products are not listed.
J. Ignacio Gutiérrez, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 100 uM Gal4–VP16 (Protein One, P1019-02) and 100 uM ATP or AMP-PNP ...
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No products found
because this supplier's products are not listed.
Justin Judd, Jonathan Lovas, Guo N. Huang,
bioRxiv - Developmental Biology 2019
Quote:
... Proteins were then transferred to PVDF-Plus membranes (Spectrum Chemical) and blocked in 5% milk in TBST (0.1% Tween 20) ...
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No products found
because this supplier's products are not listed.
Shunsuke Mori, Tetsuya Nakamura,
bioRxiv - Developmental Biology 2021
Quote:
... was used for RfxCas13d protein production (Bon Opus Biosciences, LLC). The final concentration of RfxCas13d was adjusted to 3 µg/µl in storage buffer (50 mM HEPES-KOH ...
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No products found
because this supplier's products are not listed.
Alicia Kairouani, et al.,
bioRxiv - Plant Biology 2023
Quote:
... proteins were extracted using a Constant cell disrupter system (Constant Systems) with a disruption pressure of 2.35kbar and then purified using His-bind buffer kit (Millipore ...
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No products found
because this supplier's products are not listed.
Qian Guo, et al.,
bioRxiv - Cell Biology 2024
Quote:
... goat polyclonal antibody EEA1 (A121550, Antibodies.com) (1:200 for IF).
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No products found
because this supplier's products are not listed.
Julia I. Ries, et al.,
bioRxiv - Microbiology 2022
Quote:
... The monoclonal anti-FD antibody was from BioPorto Diagnostics A/S ...
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No products found
because this supplier's products are not listed.
Fei Mao, et al.,
bioRxiv - Neuroscience 2021
Quote:
... anti-GAPDH antibody (catalog # 2-RGM2, Advanced ImmunoChemical) was used at 1 μg/mL.
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No products found
because this supplier's products are not listed.
Zsuzsa Csobán-Szabó, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Polyclonal antibody to human epidermal transglutaminase (TG3) (Zedira) (1/2000) ...
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No products found
because this supplier's products are not listed.
Karen M. Dunkerley, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Protein phosphorylation was monitored to completion using Phos-Tag AAL™ (NARD Institute Ltd) SDS–PAGE ...
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No products found
because this supplier's products are not listed.
Jérémie Courraud, et al.,
bioRxiv - Genetics 2022
Quote:
... specific primary antibodies were used: anti-UPF3B (NSJ BIOREAGENTS), anti-PQBP1 (NOVUSBIO) ...
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No products found
because this supplier's products are not listed.
Lei Liu, et al.,
bioRxiv - Immunology 2022
Quote:
... The following antibodies were used: anti-CD11b (Biogems, CA), anti-F4/80 (Biolegend ...
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No products found
because this supplier's products are not listed.
M.M. Joglekar, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... VCAN (1:200, Mouse Anti-Versican Antibody 2B1, Seikagaku), and ELN (1:400 ...
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No products found
because this supplier's products are not listed.
Jack L. Scanlan, et al.,
bioRxiv - Genetics 2020
Quote:
... melanogaster EcKL protein sequences as queries and were annotated in Artemis (Carver et al., 2012). Gene model annotation was performed by reciprocally performing BLASTP and TBLASTN searches (Altschul et al. ...
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No products found
because this supplier's products are not listed.
Catherine S. Millar-Haskell, et al.,
bioRxiv - Bioengineering 2022
Quote:
ECM proteins were covalently attached to PAA using the crosslinker Sulfo-SANPAH (Covachem; 13414-100). To begin ...
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No products found
because this supplier's products are not listed.
Tali Kiperman, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Antibodies used were diluted in 5% milk (Labscientific CN: M0841). Primary and secondary antibodies with dilutions used are described in Suppl ...
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No products found
because this supplier's products are not listed.
Aniuska Becerra-Artiles, et al.,
bioRxiv - Immunology 2022
Quote:
... and MHC proteins were removed using a Vydac C18 microspin column (The Nest Group, Ipswich, MA). The mixture of MHC and peptides were bound to the column ...
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No products found
because this supplier's products are not listed.
Transito Garcia-Garcia, et al.,
bioRxiv - Microbiology 2020
Quote:
... Antibodies against the HA and SNAP epitope were purchased from Osenses and New England BioLabs ...
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No products found
because this supplier's products are not listed.
Fabrizio Clarelli, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Primary antibodies raised against GyrA (Rabbit α-Gyrase A, PA005, Inspiralis), GyrB (Rabbit α-Gyrase B ...
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No products found
because this supplier's products are not listed.
Michelle Zuo, et al.,
bioRxiv - Immunology 2021
Quote:
... magnetic beads were conjugated with monoclonal capture antibodies (mAB47:3, UmanDiagnostics), incubated with diluted mouse serum (1:8 or 1:16 dilution ...
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No products found
because this supplier's products are not listed.
Marie S. Prevost, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Viral titres were estimated by quantification of the p24 capsid protein using HIV-1 p24 antigen immunoassay (ZeptoMetrix) and by Fluorescence Activated Cell Sorting (FACS).
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No products found
because this supplier's products are not listed.
Kimberley El, et al.,
bioRxiv - Cell Biology 2023
Quote:
... levels were normalized to total protein levels (Sigma-Aldrich, #E3138, 1:1000 with Nacalai USA Signal Enhancer HIKARI), and total (i.e ...
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No products found
because this supplier's products are not listed.
Vagelis Rinotas, et al.,
bioRxiv - Immunology 2023
Quote:
... Collagen-Antibody-Induced-Arthritis (CAIA) was induced according to manufacture instructions (MD Biosciences). Mice were maintained on a C57BL/6J genetic background.
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No products found
because this supplier's products are not listed.
Ye Bi, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Colorectal and pancreatic cancer cell lines were transduced using red fluorescent protein (RFP) and puromycin-encoding lentiviral particles obtained from GenTarget Inc ...
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No products found
because this supplier's products are not listed.
Yafei Liu, et al.,
bioRxiv - Microbiology 2020
Quote:
A pME18S expression plasmid containing the full-length or subdomain spike protein was transiently transfected into HEK293T cells using PEI max (PolyScience); the pMx-GFP expression plasmids were used as the marker of transfected cells ...
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No products found
because this supplier's products are not listed.
Sarah C. Donnelly, et al.,
bioRxiv - Microbiology 2022
Quote:
... samples containing 1–3 mg/mL of protein were evaluated using ICP-MS (Biotron Analytical Services, Western University, London, Canada). Briefly ...
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No products found
because this supplier's products are not listed.
Scott Birks, et al.,
bioRxiv - Bioengineering 2023
Quote:
... prior to being tagged with a rabbit anti-nesprin2 antibody (1:300; ImmuQuest IQ565). After primary antibody tagging ...
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No products found
because this supplier's products are not listed.
Irène Amblard, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Furimazin (1/500) was added to live cells and luciferase activity of the internalized protein was measured on a plate reader (Tristar, Berthold).
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No products found
because this supplier's products are not listed.
Michael Adams, et al.,
bioRxiv - Biochemistry 2021
Quote:
... with peptides generated from roughly 500ng of proteins injected for each analysis onto either 75 micron x 25cm packed emitter columns (New Objective) or 75 micron x 50 cm C18 Acclaim Pepmap columns (Thermo Scientific) ...
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No products found
because this supplier's products are not listed.
Martin F. Peter, et al.,
bioRxiv - Biophysics 2020
Quote:
... the protein eluate was treated with 5 times excess per cysteine of the nitroxide spin label MTSSL (Toronto Research Chemicals, Canada), dissolved in DMSO ...
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No products found
because this supplier's products are not listed.
Tiberiu Loredan Stan, et al.,
bioRxiv - Genetics 2023
Quote:
Protein was isolated from muscle samples by homogenizing the muscles in 1.4 mm Zirconium Beads prefilled tubes (OPS Diagnostics; Lebanon, USA), containing 100 mM Tris-HCl (pH 6.8 ...
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No products found
because this supplier's products are not listed.
Lu Lu, et al.,
bioRxiv - Biochemistry 2023
Quote:
Quantitative detection of milk protein in bovine milk-derived exosomes was performed according to the manufacturer’s instructions (RIDASCREEN FAST Milk kit, R-Biopharm, Germany). Quantitative detection of IgM/IgG in exosomes was performed according to the manufacturer’s instructions (IgM/IgG cow ELISA kit ...
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No products found
because this supplier's products are not listed.
Maximilian Seurig, et al.,
bioRxiv - Biochemistry 2019
Quote:
... and 13 μL of 27mM mal-PEG (Methoxypolyethylene glycol maleimide, typically 5kDa from Sigma, or 2 kDa from Nanocs, NY, for protein Q1MPU8). The samples were incubated with continuous mixing at 30°C for 1 h and the reaction was quenched by mixing with 17.5 μL 5x Sample Buffer (120 mM Tris–HCl pH 6.8 ...
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No products found
because this supplier's products are not listed.
Alberto Domingo López-Muñoz, et al.,
bioRxiv - Microbiology 2023
Quote:
... alone or in combination with purified recombinant proteins were placed in the lower chamber of a 96-well ChemoTx System plate (Neuro Probe # 101-5) in RPMI 1640 1 % FBS ...
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No products found
because this supplier's products are not listed.
Jie Li, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... or 100 ug of the highly substituted hapten NP (NP16 to NP32) conjugated to the carrier protein ovalbumin (OVA) (Biosearch Technologies; N-5051) absorbed to Imject Alum Adjuvant (ThermoFisher ...
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No products found
because this supplier's products are not listed.
Rahul Kumar, et al.,
bioRxiv - Cell Biology 2023
Quote:
... T7-RILP proteins were expressed in Escherichia coli BL21 (500 μM isopropyl β-d-1-thiogalactopyranoside; Wisent Bioproducts; at room temperature for 16 hours) and purified using standard procedure in tris buffer [20 mM tris (pH 7.4) ...
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No products found
because this supplier's products are not listed.
Maria Zhivagui, et al.,
bioRxiv - Cancer Biology 2022
Quote:
Immunofluorescence staining was carried out using anti-cyclobutane pyrimidine dimers (CPDs) monoclonal antibodies (clone KTM53, Kamiya Biomedical) and anti-6-4 photoproducts monoclonal antibodies (Clone 64M-2 ...
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No products found
because this supplier's products are not listed.
Sophie E. Cousineau, et al.,
bioRxiv - Microbiology 2022
Quote:
... slides were fixed in 100% acetone and stained with anti-HCV core antibody (1:100, clone B2, Anogen), and subsequently with the AlexaFluor-488-conjugated anti-mouse antibody (1:200 ...
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No products found
because this supplier's products are not listed.
Jayne E. Wiarda, et al.,
bioRxiv - Immunology 2022
Quote:
... mouse α-pig γδTCR-iFluor594 (primary antibody Washington State University PG2032; custom conjugation to iFluor594 performed by Caprico Biotechnologies); mouse α-pig CD4-PerCP-Cy5.5 (BD 561474) ...
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BCA Protein Colorimetric Detection assay Kit
Cat# K041-H1,
1.0 ea, USD $220.0
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Moisés dos Santos Corrêa, et al.,
bioRxiv - Animal Behavior and Cognition 2019
Quote:
... which allows detection of specific antibodies in blood plasma using the Corticosterone Enzyme Immunoassay Kit (Arbor Assays LLC, MI, USA). This kit is supplied with clear plastic microtiter plates coated with donkey anti-sheep IgG ...
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No products found
because this supplier's products are not listed.
Jacqueline M. Tokarew, et al.,
bioRxiv - Neuroscience 2020
Quote:
... A 0.4 % horseradish peroxidase solution was prepared using HRP-linked anti-rabbit secondary antibody diluted in Stabilizyme solution (SurModics SZ02). Each read was set up in triplicate on a white polystyrene 96-well plate (ThermoFisher 236105 ...
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No products found
because this supplier's products are not listed.
Emanuel Rognoni, et al.,
bioRxiv - Cell Biology 2021
Quote:
... blocked with 5% BSA/PBS (1 h at room temperature) and stained with the indicated primary antibodies and 5 µM B-CHP (BIO300, 3Helix) overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Zhengtang Qi, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The membrane was blocked for 1 h at room temperature followed by incubation overnight at 4°C with primary antibodies including FAM132b (AVISCERA BIOSCIENCE), PI3K ...