-
No products found
because this supplier's products are not listed.
Jordan M. Osmond, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and 10ng/mL of Macrophage Colony-Stimulating Factor (M-CSF) (Cedarlane/Peprotech). 10µL of the cell suspension was then added to 90µL of trypan blue (Sigma Life Science ...
-
No products found
because this supplier's products are not listed.
Brian L. Hie, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
-
No products found
because this supplier's products are not listed.
Federico Bertoglio, et al.,
bioRxiv - Biochemistry 2020
Quote:
... S1-His or RBD-His were labelled using Monolith NTTM His-Tag Labeling Kit RED-tris-NTA (Nanotemper) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Brian Belardi, et al.,
bioRxiv - Cell Biology 2019
Quote:
... HEK293 cells were transfected with TransIT-293 (Mirus) according to manufacturer’s instructions with three plasmids ...
-
No products found
because this supplier's products are not listed.
Saroj Khadka, et al.,
bioRxiv - Microbiology 2022
Quote:
... or 1:5,000 mouse anti-His-Tag (primary) then 1:5000 goat anti-mouse IgG(H+L)-HRP (secondary) (SouthernBiotech). Blots were developed with ECL Western Blotting Substrate (Pierce ...
-
No products found
because this supplier's products are not listed.
Mallory Paynich Murray, et al.,
bioRxiv - Immunology 2021
Quote:
... GM-CSF-/- (Csf2-/-) and Itgax-cre (Cd11c-cre) mouse strains were purchased from Jackson Laboratory (Bar Harbor, ME). Cd1df/f mice (Birkholz et al. ...
-
No products found
because this supplier's products are not listed.
Carolin Fritz, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His (MP Biomedicals) plates ...
-
No products found
because this supplier's products are not listed.
Joseph de Rutte, et al.,
bioRxiv - Bioengineering 2021
Quote:
... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
-
No products found
because this supplier's products are not listed.
Masaya Yamaguchi, et al.,
bioRxiv - Microbiology 2019
Quote:
Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial ...
-
No products found
because this supplier's products are not listed.
Olga S. Strelkova, et al.,
bioRxiv - Neuroscience 2024
Quote:
Mouse cochleae were fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer (EMS, #15960) supplemented with 2 mM CaCl2 for 1.5-2 hours at room temperature ...
-
No products found
because this supplier's products are not listed.
Geoffrey L. Rogers, et al.,
bioRxiv - Immunology 2023
Quote:
... 1 µg of His-tagged HIV-1 JR-CSF gp120 (Immune Technology) was added to cells for 15 min at room temperature ...
-
No products found
because this supplier's products are not listed.
Amanda Brady, et al.,
bioRxiv - Microbiology 2023
Quote:
... or with 10 ng/mL of M-CSF (Kingfisher Biotech; Cat. No. RP0462M) throughout the differentiation ...
-
No products found
because this supplier's products are not listed.
Cheng-Tsung Pan, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... A mouse 6X His tag antibody (HRP) (GeneTex Inc.) was then used to recognize Gal3-His at room temperature for 1 hour ...
-
No products found
because this supplier's products are not listed.
Egor Dzyubenko, et al.,
bioRxiv - Neuroscience 2020
Quote:
... mouse anti-neurofilament M (1:500, 171231, Synaptic Systems) and rabbit anti-Kv3.1b (1:1000 ...
-
No products found
because this supplier's products are not listed.
Vishant Boradia, et al.,
bioRxiv - Microbiology 2022
Quote:
... Blots were probed with mouse α-His antibody followed by IRDye® 680RD Goat α-Mouse IgG Secondary Antibody (LI-COR) to detect the His tagged PE15 ...
-
No products found
because this supplier's products are not listed.
Dmitry Ter-Ovanesyan, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Pooled human CSF samples were ordered from BioIVT or Biochemed Services ...
-
No products found
because this supplier's products are not listed.
Sergey A. Shiryaev, et al.,
bioRxiv - Immunology 2023
Quote:
... Horseradish peroxidase-conjugated donkey anti-mouse IgGs and TMB/M substrate were from Jackson ImmunoResearch Laboratories (West Grove ...
-
No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were seeded in 384-well plates (Greiner, #781946) at 30 000 cells/well ...
-
No products found
because this supplier's products are not listed.
Sarah N. Zvornicanin, et al.,
bioRxiv - Biochemistry 2023
Quote:
HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
-
No products found
because this supplier's products are not listed.
Samantha Pierson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Rodent Block M (Biocare) was added for 20 minutes before 2 × 5-minute 1X Dako buffer washes ...
-
No products found
because this supplier's products are not listed.
Oksana Tsyklauri, et al.,
bioRxiv - Immunology 2022
Quote:
... DM6000-M microscope (Leica Microsystems) was used to acquire images.
-
No products found
because this supplier's products are not listed.
Weisong Zhao, et al.,
bioRxiv - Biophysics 2022
Quote:
... equipped with an objective (100×/1.7 HI oil, APON, Olympus)and a sCMOS (Flash 4.0 V3 ...
-
No products found
because this supplier's products are not listed.
Sangsin Lee, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Infinite M Plex microplate reader (Tecan) was used to inject 50 μl of the assay buffer containing CTZ and measure the photon emission integrated over 30 s ...
-
No products found
because this supplier's products are not listed.
Wansu Qiu, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Sections were then rinsed 3 x 10 min in 0.1 M TBS and transferred to a secondary antibody solution with 1:200 anti-mouse IgG (Vector Laboratories, Burlington, ON, Canada) in TBS+ for 4 h at 4ºC ...
-
No products found
because this supplier's products are not listed.
Kerstin Voelkl, et al.,
bioRxiv - Neuroscience 2023
Quote:
... mouse anti-His (Dianova, DIA-900-100, 1:1,000), chicken anti-EGFP (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Mara P. Steinkamp, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human M-CSF and VEGF-A levels were measured in patient and PDX cell-free ascites fluid using the Human M-CSF ELISA Kit or Human VEGF-A ELISA kit respectively (RayBiotech, Peachtree Corners, GA). HuPDX cytokine levels were compared to published LINCOplex microarray data from paired ascites and plasma samples from ovarian cancer25 ...
-
No products found
because this supplier's products are not listed.
Pierre Gillotay, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... anti-monomeric Kusabira-Orange 2 (mouse, MBL amalgaam M-168-3M) at 1:200 ...
-
No products found
because this supplier's products are not listed.
Michael A. Kovacs, et al.,
bioRxiv - Immunology 2022
Quote:
... diluted in artificial CSF (aCSF, Harvard Apparatus) was introduced into the subarachnoid space of ligated or sham-operated mice by intra-cisterna magna (i.c.m. ...
-
No products found
because this supplier's products are not listed.
Li Xu, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Total RNA was extracted from mouse tissues and HEK293 cells using Quick-RNA MiniPrep Kit (ZYMO Research, Irvine, CA). Five μg of treated RNA was used as template for first-strand cDNA synthesis by using RevertAid RT Reverse Transcription Kit (Life Technologies ...
-
No products found
because this supplier's products are not listed.
Jia Xuan Leong, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
-
No products found
Wei Dong, et al.,
bioRxiv - Cell Biology 2020
Quote:
HEK293 cells were cultured in glass bottom dishes (In Vitro Scientific) and were transfected with DNA using X-treme Gene 9 DNA transfection reagent (Sigma Cat# 6365787001) ...
-
No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a microplate reader (BioTek Synergy HI) to record the absorbance at 650 nm ...
-
No products found
because this supplier's products are not listed.
Xinrui Wang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His-tone H2A.Z (1:200, Active Motif #39013), Nkx2.5 (1:250 ...
-
No products found
because this supplier's products are not listed.
Ashley A. Johnson, et al.,
bioRxiv - Physiology 2021
Quote:
... Spectra were measured from HEK293 cells using a 60x objective with NA 1.45 (Nikon) and an inverted microscope (Nikon TE-2000) ...
-
No products found
because this supplier's products are not listed.
Markus Frey, et al.,
bioRxiv - Neuroscience 2020
Quote:
Sound-evoked neuronal responses were obtained via chronically-implanted electrodes in the right hemisphere auditory cortex of one 17-week-old male mouse (M. musculus, C57Bl/6, Charles River). All experimental procedures were carried out in accordance with the institutional animal welfare guidelines and a UK Home OZce Project License approved under the United Kingdom Animals (Scienti1c Procedures ...
-
No products found
because this supplier's products are not listed.
Liliana S. O. Silva, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 25 % PEG 4K and 0.2 M MgCl2 plus the additive NaCl (2 M) (Hampton Research). Crystals of YtfEM-E159L were prepared by the hanging drop vapour diffusion method using 1:1 μL mixtures of protein-reservoir solution ...
-
No products found
because this supplier's products are not listed.
Patrick Blatt, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Rabbit anti-His (1:000, Rockland Inc., 600-401-382), Mouse anti-Fibrillarin (1:25 ...
-
No products found
because this supplier's products are not listed.
Andrew G. DeMarco, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 0.6 M sodium cyanoborohydride (Alfa Aesar), and either 0.2% formaldehyde (CH2O ...
-
No products found
because this supplier's products are not listed.
James S. Davies, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... The best diffracting crystals were grown in the E1 condition from the Shotgun screen (2.0 M ammonium sulfate, 0.1 M Bis-Tris pH 5.0) (Molecular Dimensions). Crystals were cryo-protected by soaking in reservoir solution supplemented with liquid glycerol and ethylene glycol.
-
No products found
because this supplier's products are not listed.
Nicole Kiweler, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Gas chromatograph was equipped with a 30 m (I.D. 250 μm, film 0.25 μm) ZB-35MS capillary column with 5 m guard column (Phenomenex). Helium was used as carrier gas with a constant flow rate of 1.2 ml/min ...
-
No products found
because this supplier's products are not listed.
Arun Prasath Damodaran, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... mouse anti-His tag (HIS.H8 / EH158, Covalab, 1:2500), mouse anti-FLAG tag (clone M2-F1804 ...
-
No products found
because this supplier's products are not listed.
Caterina Prelli Bozzo, et al.,
bioRxiv - Microbiology 2020
Quote:
... VSV-M (Mouse Monoclonal Anti-VSV-M Absolute antibody, ABAAb01404-21.0, 1:1000), actin (Anti-beta Actin antibody Abcam ...
-
No products found
because this supplier's products are not listed.
Han-Byul Kim, et al.,
bioRxiv - Neuroscience 2023
Quote:
... pooled human CSFs (Innovative Research) were mixed with 0.5% NP40 and 50 μl of Pierce Protein A/G Plus Agarose (Thermo ...
-
No products found
because this supplier's products are not listed.
Trine Lisberg Toft-Bertelsen, et al.,
bioRxiv - Neuroscience 2022
Quote:
... CSF was collected in polypropylene tubes (Sarstedt), centrifuged at 2000 x g for 10 min at 4°C ...
-
No products found
Kirandeep K. Deol, et al.,
bioRxiv - Biochemistry 2020
Quote:
HEK293 Expressing Rpn11-HTBH (Applied Biological Materials, T6007)
-
No products found
because this supplier's products are not listed.
Pablo Alcón, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ...
-
No products found
because this supplier's products are not listed.
Amrita Sule, et al.,
bioRxiv - Cancer Biology 2021
Quote:
YFP-ATM was immunoprecipitated from stably transfected HEK293 cells by GFP-TRAP (Chromotek). The immunoprecipitates were suspended in kinase assay buffer containing 2 μCi of [γ-32P] ATP ...
-
No products found
because this supplier's products are not listed.
Daniel W. Bellott, et al.,
bioRxiv - Genomics 2021
Quote:
Parafilm M film (Bemis, PM996)
-
No products found
because this supplier's products are not listed.
Prasanna Babu Araveti, et al.,
bioRxiv - Microbiology 2022
Quote:
... the fluorescence images of the HEK293 cells were captured using Axio Observer 7 microscope Apotome 2 (Carl Zeiss).
-
No products found
because this supplier's products are not listed.
Yanyan Geng, et al.,
bioRxiv - Neuroscience 2022
Quote:
Whole cell recordings were obtained from HEK293 cells using an Axopatch 200B amplifier (Molecular Devices, San Jose, CA). Recordings were filtered at 5 kHz with the amplifier internal filter and digitized at 50 kHz using a Digidata 1550B digitizer (Molecular Devices ...