-
No products found
because this supplier's products are not listed.
Jianfang Li, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Human C-peptide levels in isolated plasma were quantified using the STELLUX Chemi Human C-peptide ELISA kit (ALPCO Diagnostics) according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
A. B Dippel, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... or 50 µM c-di-GMP [in screening buffer (-lysozyme, -PMSF)] in opaque white 96-well LUMITRAC 600 plates (Greiner) to generate final concentrations of 0 ...
-
No products found
because this supplier's products are not listed.
Alessandra Gallinaro, et al.,
bioRxiv - Immunology 2021
Quote:
... were coated with 2.5 µg/ml of mouse anti-human c-Myc 9E10 mAb (produced in house) or goat anti-human IgG (1:2000, Southern Biotech) for the standard ...
-
No products found
because this supplier's products are not listed.
Michelle E. Gray, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and incubated overnight at 4°C with goat anti-human IgG (1:200; Jackson ImmunoResearch Laboratories ...
-
No products found
because this supplier's products are not listed.
Olga Mitrofanova, et al.,
bioRxiv - Bioengineering 2023
Quote:
... lysozyme (1:50, Genetex GTX72913), mucin 2 (1:50 ...
-
No products found
because this supplier's products are not listed.
Andreas Schmitt, et al.,
bioRxiv - Biochemistry 2020
Quote:
The his-tagged protein was purified at 20 °C on Ni-NTA-Sepharose (Macherey-Nagel). The column was washed with 10 column volumes (CV ...
-
No products found
because this supplier's products are not listed.
Xi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or plastic-bottom (Ibidi: DLD1, HEK293) 96-well plates at densities targeting 60-70% confluence at the time of transfection ...
-
No products found
because this supplier's products are not listed.
Daniel C. Volke, et al.,
bioRxiv - Systems Biology 2021
Quote:
... The cultures were incubated shaken at 30°C in a Synergy HI plate reader (BioTek Instruments) and growth and fluorescence were followed by spectrophotometry at 600 nm and 500/530 nm (excitation/emission wavelength) ...
-
No products found
because this supplier's products are not listed.
Kevin R. Bewley, et al.,
bioRxiv - Pathology 2020
Quote:
... were coated overnight at 4°C with rabbit anti-human IgG (Jackson Laboratories) at 5 μg/mL in PBS ...
-
No products found
because this supplier's products are not listed.
Mona Bensalah, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
-
No products found
because this supplier's products are not listed.
Huang Zhu, Dan S. Kaufman,
bioRxiv - Immunology 2019
Quote:
... 15 % heat-inactivated human AB serum (Valley Biomedical, Catalog # HP1022 HI),1 % P/S ...
-
No products found
because this supplier's products are not listed.
Tanya J. Espino-Sanchez, et al.,
bioRxiv - Microbiology 2023
Quote:
... and egg-white lysozyme (Goldbio). After a 20 min incubation at 4° C ...
-
No products found
because this supplier's products are not listed.
Danqi Sheng, et al.,
bioRxiv - Biophysics 2023
Quote:
... HEK293 cells were recorded at room temperature (25 ± 2 °C) using an Axopatch 200B amplifier (Molecular Devices, USA) with a holding potential of -60 mV ...
-
No products found
because this supplier's products are not listed.
Yohei Nishikawa, et al.,
bioRxiv - Genomics 2022
Quote:
Two hundred microliters of lysozyme solution (50 U/μL Ready-lyse lysozyme [Lucigen, WI, USA], 2 U/mL zymolyase [Zymo Research ...
-
No products found
because this supplier's products are not listed.
Carole Seguin-Devaux, et al.,
bioRxiv - Immunology 2024
Quote:
... or rabbit anti-His (Bethyl, ImTec Diagnostic NV ...
-
No products found
because this supplier's products are not listed.
Steven J. Kunnen, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... in INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT ...
-
No products found
because this supplier's products are not listed.
Shu Liu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Human primary astrocytes (ScienCell) were cultivated as recommended by ScienCell ...
-
No products found
because this supplier's products are not listed.
Frederik Fleissner, et al.,
bioRxiv - Biophysics 2020
Quote:
A plasmid coding for human vimentin containing a C-terminal His-tag (EX-D0114-B31, Tebu-bio, Germany) was transformed into E ...
-
No products found
because this supplier's products are not listed.
Greg J. Dodge, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 0.06 mg/mL lysozyme (RPI), and 0.5 mg/mL DNase I (Millipore Sigma) ...
-
No products found
because this supplier's products are not listed.
Robbin A. Nameki, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... KURAMOCHI and OVCAR4 Hi-C were conducted and processed by Active Motif using the Arima-Hi-C kit and library prep performed with technical duplicates (Arima Genomics) ...
-
No products found
because this supplier's products are not listed.
Ao Dong, et al.,
bioRxiv - Neuroscience 2020
Quote:
... His (Tocris), Epi (Sigma-Aldrich) ...
-
No products found
Kirandeep K. Deol, et al.,
bioRxiv - Biochemistry 2020
Quote:
HEK293 Expressing Rpn11-HTBH (Applied Biological Materials, T6007)
-
No products found
because this supplier's products are not listed.
Pablo Rougerie, Dianne Cox,
bioRxiv - Cell Biology 2020
Quote:
... The gel was then immediately rinsed with distilled water and incubated overnight at 4°C with a 1mg/mL solution of human whole IgG (CellSciences) or human Fab fragment (Rockland) in 50mM HEPES pH 8 ...
-
No products found
because this supplier's products are not listed.
Vilde Leipart, et al.,
bioRxiv - Biochemistry 2022
Quote:
... We made three sets of blank samples: buffer blank and two sets with SUMO-1 (human, His-tag, Enzo Life Sciences). One collection of SUMO-1 samples were incubated with 25uM ZnCl2 at 4 °C for 1 hour ...
-
No products found
because this supplier's products are not listed.
D. Azimova, et al.,
bioRxiv - Microbiology 2021
Quote:
... mouse anti-His (ABclonal AE003).
-
No products found
because this supplier's products are not listed.
Amanda M. Travis, et al.,
bioRxiv - Cell Biology 2022
Quote:
... and C-terminal MYC tag was placed in front of human NPHP3 (GeneCopoeia GC-H2370). All mutations to replace the lipidated cysteine with alanine were made using PCR mutagenesis (Weiner et al. ...
-
No products found
because this supplier's products are not listed.
Ashley A. Johnson, et al.,
bioRxiv - Physiology 2021
Quote:
... Spectra were measured from HEK293 cells using a 60x objective with NA 1.45 (Nikon) and an inverted microscope (Nikon TE-2000) ...
-
No products found
because this supplier's products are not listed.
Tetsuhiro Harimoto, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... 150 μL of blood from the single donor human whole blood or murine (BALB/c) whole blood (Innovative Research) were aliquoted into 3 wells/strain in a 96-well plate ...
-
No products found
because this supplier's products are not listed.
Niko Schwenzer, et al.,
bioRxiv - Cell Biology 2024
Quote:
HEK293 cells with constitutive expression of CaV subunits β3 and α2δ1 and inducible expression of α1D (Charles River Laboratories CT6232) were cultured in DMEM/F12 medium containing selection antibiotics and 0.6 µM isradipine (Sigma I6658) ...
-
No products found
because this supplier's products are not listed.
Victoria G. Weis, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and Rabbit anti-Lysozyme (Cat# MBS2556232, MyBioSource, CA). After 3 washes in 1X phosphate-buffered saline for 5 minutes each ...
-
No products found
because this supplier's products are not listed.
Konrad Gronke, et al.,
bioRxiv - Immunology 2023
Quote:
For treatment with lysozyme (1 mg/mL, Alfa Aesar) or proteinase K (Roche) ...
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Alina Remeeva, et al.,
bioRxiv - Biophysics 2021
Quote:
... with the mutation C85A and C-terminal 6×His tag) was obtained as a synthetic gene from Evrogen (Russia) and introduced into the pET11 expression vector (Novagen ...
-
No products found
Wei Dong, et al.,
bioRxiv - Cell Biology 2020
Quote:
HEK293 cells were cultured in glass bottom dishes (In Vitro Scientific) and were transfected with DNA using X-treme Gene 9 DNA transfection reagent (Sigma Cat# 6365787001) ...
-
No products found
because this supplier's products are not listed.
Loyda M. Morales Rodríguez, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
HEK293 cells were plated onto 25mm coverslips (Electron Microscopy Sciences, #50949050) coated with poly-D-lysine (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
Sandra D. Chanez-Paredes, et al.,
bioRxiv - Cell Biology 2023
Quote:
... mouse anti-6-His (Bio X Cell, AB_2687790) was used for western blot (3 µg/ml) ...
-
No products found
because this supplier's products are not listed.
Bekir Altas, et al.,
bioRxiv - Neuroscience 2019
Quote:
... 1 µM His-Ubiquitin (Boston Biochem, Cat#U-530), 50 nM mouse E1 enzyme ...
-
No products found
because this supplier's products are not listed.
Ivan Psakhye, et al.,
bioRxiv - Cell Biology 2022
Quote:
... resuspended in 6 ml lysis buffer (1X PBS, 500 mM NaCl, 1% Triton X-100, lysozyme, Calbiochem EDTA-free protease inhibitor cocktail set III ...
-
No products found
because this supplier's products are not listed.
Weisong Zhao, et al.,
bioRxiv - Biophysics 2022
Quote:
... equipped with an objective (100×/1.7 HI oil, APON, Olympus)and a sCMOS (Flash 4.0 V3 ...
-
No products found
because this supplier's products are not listed.
Satyaveni Malasala, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and His-DBS (DH/PH domain) were purchased from Cytoskeleton, Inc ...
-
No products found
because this supplier's products are not listed.
Suzanne M. Castenmiller, et al.,
bioRxiv - Immunology 2024
Quote:
1-3×106 cells were cultured in 24-well plates at 37°C and 5% CO2 in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
-
No products found
because this supplier's products are not listed.
Oktawia Nilsson, et al.,
bioRxiv - Biochemistry 2020
Quote:
... serum samples were centrifuged at 10000 rpm for 20 minutes at 4 °C and supernatants were analyzed by human ApoA-I ELISA (3710-1HP-10, Mabtech Inc.), as well as by denaturant and native western blot (procedure is described in the Supplementary Information file).
-
No products found
because this supplier's products are not listed.
James D. O’Leary, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and anti-c-Fos (Anti-c-Fos rabbit, 1:500, Synaptic System). Sections were then incubated in the appropriate AlexaFluor secondary antibody (AF488 for eYFP and AF596 for c-Fos ...
-
No products found
because this supplier's products are not listed.
Charles B. Reilly, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Lysozyme assay was performed using the manufacturer’s instructions (Biovision Lysozyme inhibitor screening kit K237). Flavonoids were acquired from Sigma-Aldrich and Seleckchem.
-
No products found
because this supplier's products are not listed.
Martin P. Schwalm, et al.,
bioRxiv - Biochemistry 2023
Quote:
HEK293 cells were cultured in DMEM (PAN Biotech). All media were supplemented in with 10% FBS (PAN Biotech ...
-
No products found
because this supplier's products are not listed.
Ryan J. Garrigues, et al.,
bioRxiv - Microbiology 2021
Quote:
Purified BBK32-C and a purified autolytic fragment of human C1r (Complement Technologies) were mixed at 5% molar excess BBK32-C and concentrated to 3.5 mg ml-1 in 10 mM HEPES (pH 7.3 ...
-
No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
-
No products found
because this supplier's products are not listed.
Redouane Aherrahrou, et al.,
bioRxiv - Genetics 2023
Quote:
... Human aortic SMCs (Cell Applications, Inc. ...
-
No products found
because this supplier's products are not listed.
Jessie Altieri, Klemens J. Hertel,
bioRxiv - Molecular Biology 2021
Quote:
... A metabolic labeling protocol was followed[6] and the experimental HEK293 cells were incubated in the presence of 40µM unphosphorylated 4-thiouridine (Cayman Chemical, CAY16373-5). Total RNA was harvested from the cells using Trizol (Ambion ...
-
No products found
because this supplier's products are not listed.
Antonio Barreiro, et al.,
bioRxiv - Immunology 2021
Quote:
BALB/c mice (Envigo) and transgenic B6.Cg-Tg(K18-ACE2)2Prlmn/J (K-18-hACE2 ...