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No products found
because this supplier's products are not listed.
Neil J Rzechorzek, et al.,
bioRxiv - Biochemistry 2020
Quote:
... HEK293-F cells were grown in 400 mL batches in 2 L non-baffled Erlenmeyer flasks (TriForest Enterprises Inc, #FPC2000S) to a cell density of ∼1×106 cells/mL.
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No products found
because this supplier's products are not listed.
Anne Rehkamp, et al.,
bioRxiv - Biochemistry 2020
Quote:
LC separation of peptides originating from HEK293 cell membranes was performed via selfpacked C18 columns (PicoFrits, 75 μm x 50 cm, 15 μm tip, New Objective, self-packed with ReproSil-Pur 120 C18-AQ ...
-
No products found
because this supplier's products are not listed.
Ronan Shaughnessy, et al.,
bioRxiv - Cell Biology 2023
Quote:
... lentivi-ral particles were produced in the HEK293 FT packaging cells transfected with MiniBAR constructs and the Excelenti LTX Lentivirus Packaging mix (Oxford Genetics) using lipo-fectamine following manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Rania Francis, et al.,
bioRxiv - Microbiology 2019
Quote:
Two cell lines were used as cellular supports for co-culture: the human embryonic lung fibroblast MRC5 cells (RD-Biotech, Besançon, France) and the mouse fibroblast L929 cells (ATCC® CCL-1) ...
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Cat# IT-52-250,
250 micrograms,USD $2400.0
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Lidia I. Madrid, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Infusion of murine-p75 neurotrophin receptor (p75NTR)-Saporin (hereafter abbreviated as p75-Sap; 0.4 µg/µl; Advanced Targeting System) or control rabbit-IgG-Saporin (IgG-Sap ...
-
No products found
because this supplier's products are not listed.
Katlijn De Meulenaere, et al.,
bioRxiv - Microbiology 2022
Quote:
... after which the parasites were harvested and remaining human white blood cells were depleted with Plasmodipur filtration (EuroProxima) as described by Auburn et al ...
-
No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
-
No products found
because this supplier's products are not listed.
Nirupa Nagaratnam, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The supernatant containing detergent solubilized His-tagged FopA was filtered through a 0.22 μm cellulose acetate membrane filter (Foxx Life Sciences) and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP ...
-
No products found
because this supplier's products are not listed.
Padmapriya Sekar, et al.,
bioRxiv - Immunology 2023
Quote:
... 330 μg/mL iron-saturated human holo-transferrin (BBI solutions), 10 μg/mL recombinant human insulin (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Kyle E. Landgraf, et al.,
bioRxiv - Cancer Biology 2019
Quote:
Human PBMC stimulation and immune-phenotyping studies were performed by iQ Biosciences. Briefly normal PBMCs from three donors were seeded in 96-well plates at 1×105 cells/well and exposed to a 10-fold dilution series of either U2S3-hFc-mutIL2 or U2S3-hFc-wtIL2 (wild-type IL2 ...
-
No products found
because this supplier's products are not listed.
Aaron M. Rosado, et al.,
bioRxiv - Biophysics 2022
Quote:
... freshly isolated human RBCs were biotinylated with biotin-PEG3500-NHS (Jenkem Technology) and then incubated with nystatin in N2 buffer (265.2 mM KCl ...
-
No products found
because this supplier's products are not listed.
Ghulam Destgeer, et al.,
bioRxiv - Bioengineering 2020
Quote:
... particles were incubated with varying concentrations of human recombinant NT-proBNP (HyTest, Finland) for 1 hr with subsequent washing ...
-
No products found
because this supplier's products are not listed.
Matthew J. Shannon, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... The assay was validated using a standard curve calibrated against human methylation controls (EpigenDx, USA Cat ...
-
No products found
because this supplier's products are not listed.
Jonah C. Rosch, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Fluorescently labeled human serum albumin (HS1-S5-1) was purchased from NANOCS (New York, NY). The starting DNA library ...
-
No products found
because this supplier's products are not listed.
Michal Schwartz, et al.,
bioRxiv - Microbiology 2022
Quote:
... using FAM labeled HCMV primer and probe: Human CMV HHV5 kit for qPCR using a glycoprotein B target (PrimerDesign); and HEX labeled RPP30 copy number assay for ddPCR (Bio-Rad) ...
-
No products found
because this supplier's products are not listed.
Jessica B. Blackburn, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 25 μg of SIgA was incubated with 10 μg sputum-derived human neutrophil elastase (1 ug/uL in 0.05 M NaOAc pH 5 containing 0.1 M NaCl, Elastin Products Company, SE563GI) with or without a protease inhibitor (Sigma ...
-
No products found
because this supplier's products are not listed.
Hanyuan Shen, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
A431 cells were treated with different injections for 48 hours in 96-well plates and the cell culture supernatant was collected and tested for the level of IL-1β by ELISA using human interleukin-1 beta ELISA kit (Biosensis, CA, USA) according to the kit protocol ...
-
No products found
because this supplier's products are not listed.
Xindi Li, et al.,
bioRxiv - Neuroscience 2024
Quote:
... human-derived microglia were subjected to a seven-day chronic intermittent exposure (CIE) paradigm using 200 Proof ethanol from Decon Laboratories (catalog# 2716) at concentrations of 0 mM (control) ...
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No products found
because this supplier's products are not listed.
Lukas Spantzel, et al.,
bioRxiv - Biophysics 2023
Quote:
... Cells were lysed by two passages through a PandaPlus 2000 cell homogenizer (GEA Niro Soavi, Italy) at 1000 bar ...
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No products found
because this supplier's products are not listed.
Marc-Jan Gubbels, et al.,
bioRxiv - Microbiology 2020
Quote:
... defibrinated bovine red blood cells (Hemostat Labs, Dixon, CA).
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No products found
because this supplier's products are not listed.
Susan Hromada, et al.,
bioRxiv - Systems Biology 2021
Quote:
Cells were cultured in an anaerobic chamber (Coy Lab products) with an atmosphere of 2.5±0.5% H2 ...
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No products found
because this supplier's products are not listed.
J.I. Hoare, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... All cell lines underwent 16 locus STR verification (DNA Diagnostics Centre ...
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No products found
because this supplier's products are not listed.
Anindita Sarkar, et al.,
bioRxiv - Biochemistry 2020
Quote:
... The redox titrations were carried out in an electrochemical cell (BASI) equipped with three electrodes (Au gauze as working electrode ...
-
No products found
because this supplier's products are not listed.
Anika Koetemann, Bernd Wollscheid,
bioRxiv - Cell Biology 2020
Quote:
Filter-grown cells were treated with 1 μM SF1670 (Lucerna Chem) and/or 1 μM LY29400 in 20 ml serum-free DMEM ...
-
No products found
because this supplier's products are not listed.
Jakub Gemperle, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... and cells imaged in 1x Opti-Klear medium (Marker Gene Technologies Inc) supplemented with 10% (v/v ...
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No products found
because this supplier's products are not listed.
Biao Huang, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... 20,000 cells per well were seeded in iMatrix-511 (Nacalai USA, # 892021) coated Seahorse miniplates 18-20 hours before the assay ...
-
No products found
because this supplier's products are not listed.
Cecilia Östlund, et al.,
bioRxiv - Cell Biology 2022
Quote:
... the cell lysates were homogenized with a Wheaton Dounce homogenizer (DWK Life Sciences), underlaid with a 40% sucrose cushion ...
-
Cat# 45-06-45,
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Sean P Harrison, et al.,
bioRxiv - Cell Biology 2020
Quote:
... depending on the cell line and 3 or 4 μM CHIR99021 (BOC Sciences). Optimal conditions need to be established for each line based on our previously established protocol 4 ...
-
No products found
because this supplier's products are not listed.
Filipe Nunes Vicente, et al.,
bioRxiv - Biophysics 2021
Quote:
... cells expressing Vimentin– HALO were first incubated with 90 nm gold nanoparticles (Cytodiagnostics) to serve as fiducial markers ...
-
No products found
because this supplier's products are not listed.
Matthew Teryek, et al.,
bioRxiv - Bioengineering 2023
Quote:
... cell suspensions were mixed with VitroGel-MSC (TheWell Biosciences, North Brunswick, NJ, USA) to a total volume of 6 mL in a 1:2 v/v ratio according to the manufacturer’s recommendations ...
-
No products found
because this supplier's products are not listed.
Jun Yu Ip, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... with absorbance at 254 nm monitored using a Triax Flow Cell (Biocomp Instruments). Monosome fractions were pooled and treated with 200 μg/mL Proteinase K (Roche ...
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No products found
because this supplier's products are not listed.
Dominik P. Elmer, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Production of lentiviral particles by 293FT cells using metafectene pro (Biontex Laboratories, Munich, Germany) and transduction of melanoma cells was performed following the protocol described in [66] ...
-
No products found
because this supplier's products are not listed.
Sunay Usluer, et al.,
bioRxiv - Cell Biology 2022
Quote:
... cells were passaged into media supplemented with 15 μg/ml Blasticidin (TOKU-E, B001), and selection was continued for 10 days.
-
No products found
because this supplier's products are not listed.
Lukas-Adrian Gurzeler, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... the cells were treated with 100 μg/ml Cycloheximide (Focus Biomolecules, Cat# 10-117) and incubated for 4 min to stop translation and stabilize ribosomes on the mRNAs ...
-
No products found
because this supplier's products are not listed.
Rocío del M. Saavedra-Peña, et al.,
bioRxiv - Physiology 2022
Quote:
... Cells were then stained with anti-BrdU antibody (Alexa Fluor 647; Phoenix Flow Systems; AX647) at 1:30 in HBSS with 3% BSA overnight in the dark at 4C ...
-
No products found
because this supplier's products are not listed.
Mitsuhiro Abe, et al.,
bioRxiv - Cell Biology 2024
Quote:
... the cells were incubated with 10 nM HaloTag HMSiR Ligand (#A201-01, GORYO Chemical, Inc.) in DMEM supplemented with 0.2% BSA for 16 h ...
-
No products found
because this supplier's products are not listed.
Elisa Jentho, et al.,
bioRxiv - Immunology 2019
Quote:
Stock solution of Heme (Sigma-Aldrich for cell culture experiments and Frontier Scientific for animal experiments) and Protoporphyrin 9 (PPIX ...
-
No products found
because this supplier's products are not listed.
Lijun Cong, et al.,
bioRxiv - Microbiology 2021
Quote:
... prior to cell supernatants being collected for quantification of virus production by HIV-1 p24 ELISA (XpressBio).
-
No products found
because this supplier's products are not listed.
Jason Small, Alison Weiss,
bioRxiv - Microbiology 2021
Quote:
... Cells were washed with PBST and placed in primary antibody (Rabbit Anti-E. coli, cat. 1001, ViroStat, Rabbit Anti-Intimin ...
-
No products found
because this supplier's products are not listed.
Joury S van ‘t Klooster, et al.,
bioRxiv - Biochemistry 2020
Quote:
... The resulting cell pellet was resuspended in 150 mL of cell resuspension buffer (CRB; 20mM Tris-HCl pH6.7, 1mM EGTA, 0.6M sorbitol, 10μM pepstatin A (Apollo Scientific, UK), 10μM E-64 (Apollo Scientific ...
-
No products found
because this supplier's products are not listed.
L Mancini, T Pilizota,
bioRxiv - Microbiology 2022
Quote:
... Illumination for the cells expressing pHluorin sensor was provided by a neutral white LED with the filter ET470/40x (Chroma Technology, USA) and a UV LED (Cairn ...
-
No products found
because this supplier's products are not listed.
Chafen Lu, et al.,
bioRxiv - Microbiology 2019
Quote:
... Secondary antibodies were rabbit polyclonal anti-His (Delta Biolabs), Horseradish peroxidase (HRP)-anti-rabbit and HRP-anti-mouse IgG (GE Healthcare ...
-
No products found
because this supplier's products are not listed.
Shima Shahbaz, et al.,
bioRxiv - Immunology 2020
Quote:
... The SARS-CoV-19 spike receptor binding domain protein was purchased from VIROGEN (Cat#00224-V), conjugated with dye using Fluorescent protein labeling kit according to the manufacturing protocol (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Takahiro Sanada, et al.,
bioRxiv - Microbiology 2022
Quote:
... AB human serum (Pel-Freez Biologicals, Rogers, AR, USA) was used as control serum.
-
No products found
because this supplier's products are not listed.
Guillaume N. Fiches, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 10% heat-inactivated human serum (Advanced Biotechnologies Inc, Cat#P2-203), 100 U/mL penicillin ...
-
No products found
because this supplier's products are not listed.
Stephen A. Schumacher, et al.,
bioRxiv - Physiology 2021
Quote:
Serum PTH concentrations were measured with a human-specific immunoradiometric assay (Scantibodies Laboratory, Santee, CA, USA) with a working range of 6.5-2328 pg/mL ...
-
No products found
because this supplier's products are not listed.
Hanna Englert, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... the membranes were incubated with a goat anti-human FXII antibody (1:500, GAHu/FXII, Nordic-MUbio, The Netherlands) for 1h at RT ...
-
No products found
because this supplier's products are not listed.
Kyung-Jin Jang, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
Alpha Glutathione S-Transferase (α-GST): levels were quantified in human model effluent samples from the upper channel using an ELISA kit (DiaPharma). The assay was run following the vendor protocol using a standard curve ranging from 0-64 µg/L ...
-
No products found
because this supplier's products are not listed.
Livia Mazzini, et al.,
bioRxiv - Immunology 2020
Quote:
... Human IgM anti-SARS-CoV-2 Spike (S1) Antibody CR3022 (Native Antigen, Oxford, UK) and anti-Spike RBD (SARS-CoV-2/COVID 19) human monoclonal antibody (eEnzyme, Gaithersburg, USA) were used as positive controls in ELISA ...
-
No products found
because this supplier's products are not listed.
Balázs Knakker, et al.,
bioRxiv - Animal Behavior and Cognition 2020
Quote:
... For stimulus presentation and data acquisition we used the CAmbridge Neuropsychological Test Automated Battery (CANTAB) specifically designed for non-human primates (Monkey CANTAB Cognitive Testing system with Intellistation and Whisker software, Campden Instruments, UK). The stimulus set (camcog_mdms0 ...