-
No products found
because this supplier's products are not listed.
Arnaud N’Guessan, et al.,
bioRxiv - Genomics 2022
Quote:
... and goat anti-human IgA (alpha chain) DyLight800 (Rockland Immunochemicals) antibodies at a final concentration of 0.1 μg/ml and 1 μg/ml ...
-
No products found
because this supplier's products are not listed.
Kevin Michalski, et al.,
bioRxiv - Neuroscience 2019
Quote:
HEK293 cells were plated onto 12-mm glass coverslips (VWR) in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega ...
-
No products found
because this supplier's products are not listed.
Jianfang Li, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Human C-peptide levels in isolated plasma were quantified using the STELLUX Chemi Human C-peptide ELISA kit (ALPCO Diagnostics) according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Lien Provez, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... a beta-galactosidase (SCIEX) and MPDS II (Waters Corporation ...
-
No products found
because this supplier's products are not listed.
Brian L. Hie, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
-
No products found
because this supplier's products are not listed.
Kristin M Kotewicz, et al.,
bioRxiv - Microbiology 2023
Quote:
... each 25 μl pelleted resin was resuspended with 4μg of recombinant human homotypic-linked polyubiquitin chains Ub3-7 (K63 or K48, 4μg/600μl reaction volume, Boston Biochem, R&D) and 500μM ε-NAD in 1X ART buffer for 2 hrs with gentle end-over-end rotation at 37°C ...
-
No products found
because this supplier's products are not listed.
Natalya Pashkova, et al.,
bioRxiv - Neuroscience 2023
Quote:
HEK293 cells were grown on glass bottom dishes (MatTek, P35G-1.5-20-C). After 18-20 hrs of transfection ...
-
No products found
because this supplier's products are not listed.
Ao Dong, et al.,
bioRxiv - Neuroscience 2020
Quote:
... His (Tocris), Epi (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Götz Laible, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... expressed gCetuximab light- and heavy-chains were detected with a peroxidase-conjugated anti-human IgG antibody (MP Biomedicals, Santa Ana, USA) and visualized with Bio-Rad Clarity™ Western ECL Substrate (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Tim Sen Wang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Multiubiquitin Chains (Clone FK2; Cayman Chemical), TIM23 (BD Biosciences) ...
-
No products found
because this supplier's products are not listed.
V.M. Doulames, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The samples were then incubated overnight at 4°C with primary antibody (beta-tubulin III 1:500; STEMCELL Technologies 60052) diluted in blocking buffer ...
-
No products found
because this supplier's products are not listed.
CP Profaci, et al.,
bioRxiv - Neuroscience 2024
Quote:
... The forebrain was dissected and weighed before lyophilization at a shelf temperature of –56 °C for 24h under vacuum of 0.2 mBar (Christ LMC-1 BETA 1-16) and extraction with formamide at 57°C for 24h on a shaker at 300 rpm (Eppendorf Thermomixer). Integrated density of tracer fluorescence was determined in triplicates after 1:3 ethanol dilutions ...
-
No products found
because this supplier's products are not listed.
Yogesh Singh, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; human α-syn: 804-258-1001, Enzo Life Science; beta-actin: A4700, Sigma). After incubation with the first antibody ...
-
No products found
because this supplier's products are not listed.
Andreas Schmitt, et al.,
bioRxiv - Biochemistry 2020
Quote:
The his-tagged protein was purified at 20 °C on Ni-NTA-Sepharose (Macherey-Nagel). The column was washed with 10 column volumes (CV ...
-
No products found
because this supplier's products are not listed.
Xi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or plastic-bottom (Ibidi: DLD1, HEK293) 96-well plates at densities targeting 60-70% confluence at the time of transfection ...
-
No products found
because this supplier's products are not listed.
D. Azimova, et al.,
bioRxiv - Microbiology 2021
Quote:
... mouse anti-His (ABclonal AE003).
-
No products found
because this supplier's products are not listed.
Joseph D. Kittle Jr., et al.,
bioRxiv - Bioengineering 2019
Quote:
... the human CD3 zeta chain sequence (MyBioSource Inc, San Diego, CA) was PCR-amplified and fused to the extracellular domain of mouse FcγRI (GeneCopoeia ...
-
No products found
because this supplier's products are not listed.
Carmon Kee, et al.,
bioRxiv - Immunology 2021
Quote:
Human recombinant IFN-beta 1a (IFNb) was obtained from Biomol (#86421). Recombinant human IFNλ 1 (IL-29 ...
-
No products found
because this supplier's products are not listed.
Daniel C. Volke, et al.,
bioRxiv - Systems Biology 2021
Quote:
... The cultures were incubated shaken at 30°C in a Synergy HI plate reader (BioTek Instruments) and growth and fluorescence were followed by spectrophotometry at 600 nm and 500/530 nm (excitation/emission wavelength) ...
-
No products found
Takumi Saito, et al.,
bioRxiv - Bioengineering 2020
Quote:
... and anti-beta-actin (G043, ABM) were used for detecting endogenous TAGLN2 proteins ...
-
No products found
because this supplier's products are not listed.
Tarun Mishra, et al.,
bioRxiv - Microbiology 2021
Quote:
... rabbit anti-beta actin (LI-COR Biosciences ...
-
No products found
because this supplier's products are not listed.
Tristan A. Bell, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 5% n-dodecyl-beta-maltoside (DDM) (Anatrace)) and incubated for 1 h at 4C with end-over-end rotation ...
-
No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were seeded in 384-well plates (Greiner, #781946) at 30 000 cells/well ...
-
No products found
because this supplier's products are not listed.
Huang Zhu, Dan S. Kaufman,
bioRxiv - Immunology 2019
Quote:
... 15 % heat-inactivated human AB serum (Valley Biomedical, Catalog # HP1022 HI),1 % P/S ...
-
No products found
because this supplier's products are not listed.
Robbin A. Nameki, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... KURAMOCHI and OVCAR4 Hi-C were conducted and processed by Active Motif using the Arima-Hi-C kit and library prep performed with technical duplicates (Arima Genomics) ...
-
No products found
because this supplier's products are not listed.
Danqi Sheng, et al.,
bioRxiv - Biophysics 2023
Quote:
... HEK293 cells were recorded at room temperature (25 ± 2 °C) using an Axopatch 200B amplifier (Molecular Devices, USA) with a holding potential of -60 mV ...
-
No products found
because this supplier's products are not listed.
Ramesh Rijal, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Fractionated polyP (medium chain polyP p100 and short chain polyP) were from Kerafast (Boston, MA). PolyP standards of specific chain lengths (60-mer polyP ...
-
No products found
because this supplier's products are not listed.
Steven J. Kunnen, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... in INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT ...
-
No products found
because this supplier's products are not listed.
Amanda M. Travis, et al.,
bioRxiv - Cell Biology 2022
Quote:
... and C-terminal MYC tag was placed in front of human NPHP3 (GeneCopoeia GC-H2370). All mutations to replace the lipidated cysteine with alanine were made using PCR mutagenesis (Weiner et al. ...
-
No products found
because this supplier's products are not listed.
Xin Su, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and 100μg of anti-Rat-kappa light chain (MAR18.5, BE0122; BioXCell) on day 1 ...
-
No products found
because this supplier's products are not listed.
Alina Remeeva, et al.,
bioRxiv - Biophysics 2021
Quote:
... with the mutation C85A and C-terminal 6×His tag) was obtained as a synthetic gene from Evrogen (Russia) and introduced into the pET11 expression vector (Novagen ...
-
No products found
because this supplier's products are not listed.
Ilona A. Kesisova, et al.,
bioRxiv - Cell Biology 2020
Quote:
... anti-alpha/beta tubulin (1:100; Cytoskeleton). F(ab’)2 fragment affinity-purified secondary antibodies (1:200 ...
-
No products found
because this supplier's products are not listed.
Nicholas Gladman, et al.,
bioRxiv - Plant Biology 2019
Quote:
... isopropyl-beta-D-thiogalactoside (IPTG) (Goldbio: I2481C25) was added to a final concentration of 0.001 M ...
-
No products found
because this supplier's products are not listed.
Mona Bensalah, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
-
No products found
because this supplier's products are not listed.
Wataru Shihoya, et al.,
bioRxiv - Biochemistry 2024
Quote:
... 1% n-dodecyl-beta-D-maltopyranoside (DDM) (Calbiochem), 0.2% CHS ...
-
No products found
because this supplier's products are not listed.
Tetsuhiro Harimoto, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... 150 μL of blood from the single donor human whole blood or murine (BALB/c) whole blood (Innovative Research) were aliquoted into 3 wells/strain in a 96-well plate ...
-
No products found
because this supplier's products are not listed.
Shu Liu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Human primary astrocytes (ScienCell) were cultivated as recommended by ScienCell ...
-
No products found
because this supplier's products are not listed.
Antonin Weckel, et al.,
bioRxiv - Microbiology 2019
Quote:
... AMP concentrations were analyzed in the same supernatants by ELISA with the following kits: LL37 (Hycult,biotech), human beta defensin 1 (R&D, Novus) and human beta defensin 2 (Elabscience). The concentrations are reported as pg/mL medium ...
-
No products found
because this supplier's products are not listed.
Wei-Li Ling, Samuel Ken-En Gan,
bioRxiv - Immunology 2022
Quote:
The heavy and light chain plasmids were then co-transfected into HEK293 cells us-ing PEI MAX® (Cat: 24765-1, PolyScience). The production supernatant was harvested af-ter 14 days post-transfection and purified using AKTA equipped with a PpL affinity col-umn (Cat ...
-
No products found
because this supplier's products are not listed.
Shang Geng, Xing Lv, Weiwei Zheng, Tianjun Xu,
bioRxiv - Immunology 2024
Quote:
... The lysate was incubated over night at 4°C with anti-His beads (Solarbio). Anti-His beads were washed subsequently with washing buffer (500 mM NaCl ...
-
No products found
because this supplier's products are not listed.
Peter M. Masschelin, et al.,
bioRxiv - Physiology 2022
Quote:
... beta-hydroxybutyrate (Biovision K632), and serum-free fatty acids (#sfa-1 ...
-
No products found
because this supplier's products are not listed.
Bjoern Traenkle, et al.,
bioRxiv - Immunology 2021
Quote:
... an alpaca (Vicugna pacos) was immunized with the purified extracellular domains of human CD4 (aa26-390) recombinantly produced in HEK293 cells (antibodies-online GmbH, Germany). After initial priming with 1 mg ...
-
No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
-
No products found
Wei Dong, et al.,
bioRxiv - Cell Biology 2020
Quote:
HEK293 cells were cultured in glass bottom dishes (In Vitro Scientific) and were transfected with DNA using X-treme Gene 9 DNA transfection reagent (Sigma Cat# 6365787001) ...
-
No products found
because this supplier's products are not listed.
Marilena Poxleitner, et al.,
bioRxiv - Immunology 2023
Quote:
... and beta-amyloid (Clone Abeta 42, Synaptic Systems, Goettingen, Germany). Appropriate positive and negative controls were used to confirm the adequacy of the staining ...
-
No products found
because this supplier's products are not listed.
Natalia Sanchez de Groot, et al.,
bioRxiv - Systems Biology 2019
Quote:
... SD -His with uracil and 5FOA (Zymo Research) was used to analyze the phase separation effects of a toxic Ura3p activity.
-
No products found
because this supplier's products are not listed.
Ashley A. Johnson, et al.,
bioRxiv - Physiology 2021
Quote:
... Spectra were measured from HEK293 cells using a 60x objective with NA 1.45 (Nikon) and an inverted microscope (Nikon TE-2000) ...
-
No products found
because this supplier's products are not listed.
Suzanne M. Castenmiller, et al.,
bioRxiv - Immunology 2024
Quote:
1-3×106 cells were cultured in 24-well plates at 37°C and 5% CO2 in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
-
No products found
because this supplier's products are not listed.
Oktawia Nilsson, et al.,
bioRxiv - Biochemistry 2020
Quote:
... serum samples were centrifuged at 10000 rpm for 20 minutes at 4 °C and supernatants were analyzed by human ApoA-I ELISA (3710-1HP-10, Mabtech Inc.), as well as by denaturant and native western blot (procedure is described in the Supplementary Information file).
-
No products found
because this supplier's products are not listed.
Anna V. Elleman, et al.,
bioRxiv - Neuroscience 2023
Quote:
... beta subunit (Cedarlane, Ontario, Canada)).41 Cells were fed every 2–4 days by changing 50% of the working medium.