-
No products found
because this supplier's products are not listed.
Rogger P. Carmen-Orozco, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and HEK-293 cells were extracted using Monarch Total RNA Miniprep Kit (New England BioLabs, T2010S). To prepare RNA-Seq libraries ...
-
No products found
because this supplier's products are not listed.
Liisa Nissinen, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... HEK and HOK (human oral keratinocytes) were from ScienCell Research Laboratories (Carlsbad ...
-
No products found
because this supplier's products are not listed.
David Andruszewski, et al.,
bioRxiv - Neuroscience 2023
Quote:
... resuspended in PBS/FCS supplemented with Fc-block (BioXCell) and incubated for 15min on ice ...
-
No products found
because this supplier's products are not listed.
Raphael Fettig, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... NIH-3T3 fibroblasts and HEK-293 cells (all from ATCC, LGC Standards GmbH, Wesel, Germany) were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Awadalkareem Adam, et al.,
bioRxiv - Immunology 2021
Quote:
... Human recombinant ACE2-Fc-tag (Raybiotech) was then added at 1 μg/mL and incubated overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Maryna V. Ivanchenko, et al.,
bioRxiv - Neuroscience 2023
Quote:
Total RNA was extracted from transduced HEK 293 cells using the Zymo Quick-RNA Microprep Kit (Zymo Research, #R1050). Reverse transcription was performed with Invitrogen’s SuperScript IV VILO Master Mix with ezDNAse enzyme (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Haizhang Chen, et al.,
bioRxiv - Immunology 2020
Quote:
... or a PE-TexasRed-conjugated human IgG-Fc fragment (Rockland) for 1h at 4°C in PBS/3%FCS ...
-
No products found
because this supplier's products are not listed.
Rogger P. Carmen-Orozco, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The following antibody was used for HEK-293 cells immunoblot: Rabbit anti-Mouse TDP-43 N-Term (Proteintech 10782-2, 1:2000).
-
No products found
because this supplier's products are not listed.
Jui-Hsien Chang, et al.,
bioRxiv - Neuroscience 2020
Quote:
... AFL was labeled at the Fc portion of human IgG immunoglobulin (goat polyclonal anti-human IgG Fc antibody, NB7446, Novus Biologicals, 1:200 at 4C overnight). Stainings were followed by a corresponding Alexa Fluor-conjugated secondary antibody (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Salim Benlefki, et al.,
bioRxiv - Neuroscience 2022
Quote:
... enhancer for ligand and recombinant human Fas-Fc were purchased from Enzo Life Sciences. z-IETD-fmk ...
-
No products found
because this supplier's products are not listed.
Julianne B. Riggs, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cells were subjected to Fc receptor blocking using Fc Shield (Tonbo Biosciences) for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Erik Procko,
bioRxiv - Biochemistry 2020
Quote:
Hydrated anti-human IgG Fc biosensors (Molecular Devices) were dipped in expression medium containing RBD-IgG1 for 60 s ...
-
No products found
because this supplier's products are not listed.
Gary Baisa, et al.,
bioRxiv - Immunology 2020
Quote:
... an RBD-Fc fragment (319-541) with a C-terminal human Fc-tag was purchased from BPS Bioscience (San Diego, CA). Recombinant biotinylated ACE2 was purchased from Acro Biosystems (Newark ...
-
No products found
because this supplier's products are not listed.
Henriette Hoffmann-Veltung, et al.,
bioRxiv - Immunology 2022
Quote:
... 100 µL of either goat anti-mouse IgG (for 6E2 hybridoma cells) or mouse anti-human IgG (for EBV-immortalized human B cells) specific for the Fc region (Mabtech) diluted in sterile PBS at 15 µg/mL were added to wells followed by incubation at 4°C for 24 hours ...
-
No products found
because this supplier's products are not listed.
Eri Takematsu, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Viruses were produced in HEK-293Ta cells using human lentiviral packaging system according to the manufacturer’s instructions (Genecopoeia). Puromycin was used to select only for transduced cells ...
-
No products found
because this supplier's products are not listed.
Miriam R. Fein, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Fc receptor blocker (Innovex Biosciences), and finally avidin/biotin blocking buffer (Vector Laboratories ...
-
No products found
because this supplier's products are not listed.
MT Heemskerk, et al.,
bioRxiv - Immunology 2021
Quote:
... diluted in PBS for 10 min at RT and Fc-receptors were subsequently blocked using 5% human serum (HS; Sanquin Blood bank, Amsterdam, The Netherlands) for 45 min at RT ...
-
No products found
because this supplier's products are not listed.
Xiaofeng Wu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
HEK-293T cells were seeded into 96-well culture plates (PerkinElmer, 6055500) at 9×103 cells/well and cultured in DMEM (Gibco ...
-
No products found
because this supplier's products are not listed.
Kazuki Hattori, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The device was presoaked in FC-40 (3M) for more than 30 min and placed in a stage top incubator (STXG-WSKMX ...
-
No products found
because this supplier's products are not listed.
Anthony A. Ruberto, et al.,
bioRxiv - Genomics 2021
Quote:
... primary human hepatocytes (BioIVT) were seeded 2-3 days prior to infection with 15,000-20,000 sporozoites per well ...
-
No products found
because this supplier's products are not listed.
Sandra Wimberger, et al.,
bioRxiv - Molecular Biology 2022
Quote:
HEK293T cells (parental clone HEK-293, ATCC CRL-1573; Genhunter corporation Q401) and HEK293T-KO cell pools (PARP1-/- ...
-
No products found
because this supplier's products are not listed.
Alexander P. Clark, et al.,
bioRxiv - Bioengineering 2021
Quote:
Human embryonic kidney cells 293 stably expressing human hyperpolarization-gated cyclic nucleotide-sensitive cation channel 1 (HEK-HCN1) were obtained from Charles River (CT6114). Cells were cultured and maintained according to the online protocol by Charles River ...
-
No products found
because this supplier's products are not listed.
Cristina Lao-Peregrin, et al.,
bioRxiv - Cell Biology 2023
Quote:
HEK 293 cells were purchased from ATCC (CRL-1573) and authenticated by Bio-Synthesis, Inc ...
-
No products found
because this supplier's products are not listed.
Maria B. Campaña, et al.,
bioRxiv - Biochemistry 2023
Quote:
Mammalian cell line HEK 293 (ATCC) was maintained in 75 mL vented tissue culture flasks (Celltreat) and kept to 70% confluency before splitting ...
-
No products found
because this supplier's products are not listed.
Cary R. Boyd-Shiwarski, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The homology arms were amplified from extracted HEK-293 cell genomic DNA (QuickExtract DNA extraction solution, Lucigen). The fidelity of all constructs was confirmed by Sanger sequencing prior to use.
-
Recombinant Human CD19 Protein, With C-Fc Tag (rh CD19 Fc Chimera) Pro 20 - Lys 291 (Accession #...
Cat# CD19-3308H,
10ug , USD $198
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Sunil Yeruva, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Recombinant protein of human DSG2 tagged with IgG Fc domain (Fc) (Creative Biomart; #DSG2-1601H) and N-CAD-Fc (Sino Biological ...
-
No products found
because this supplier's products are not listed.
Maurice Michel, et al.,
bioRxiv - Biochemistry 2024
Quote:
... goat anti-human IgG-Fc Alexa488 (Dianova, 1:400) or goat antihuman IgM Alexa 594 (Molecular Probes ...
-
No products found
because this supplier's products are not listed.
Magda Krakowczyk, et al.,
bioRxiv - Molecular Biology 2023
Quote:
HEK-293 cells (60%-70% of confluency) were fixed by 2.5% glutaraldehyde and 2% paraformaldehyde (both from Electron Microscopy Science, Inc.) solution for 1 h at 4°C ...
-
No products found
because this supplier's products are not listed.
Musleh M. Muthana, et al.,
bioRxiv - Immunology 2023
Quote:
... Azide-free human IgG-Fc was purchased from Athens Research and Technology (Athens ...
-
No products found
because this supplier's products are not listed.
Maike Pohlers, et al.,
bioRxiv - Cancer Biology 2023
Quote:
For automated dual luciferase reporter assay 2-2.5 x 104 HEK 293 T cells were seeded per 96-well by the liquid handling system epMotion 5075 (Eppendorf, Hamburg, Germany). HEK 293 T cells were transfected with 50 ng/well reporter vector with or without 3′UTR and 200 ng/well pSG5 empty vector or pSG5-miR-142-5p expression plasmid ...
-
No products found
because this supplier's products are not listed.
Sounak Chowdhury, et al.,
bioRxiv - Microbiology 2021
Quote:
... Recombinant human IgA-Fc domain (catalog number PR00105) was purchased from Absolute Antibody, UK.
-
No products found
because this supplier's products are not listed.
Taisei Wakigawa, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... BAY-293 (Tocris, 6857, 1 μM), LY294002 (Sigma‒Aldrich ...
-
No products found
Tamara Miljuš, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
For all experiments human embryonic kidney (HEK) 293SL cells84 were used and regularly tested for mycoplasma contamination (PCR Mycoplasma Detection kit, ABM, Canada). HEK293SL cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) ...
-
No products found
because this supplier's products are not listed.
Ruud H. Wijdeven, et al.,
bioRxiv - Immunology 2021
Quote:
... HEK 293T cells were transfected using polyethyleneimine (Polyscience Inc.) with packaging plasmids pRSVrev ...
-
No products found
because this supplier's products are not listed.
James Brett Case, et al.,
bioRxiv - Microbiology 2020
Quote:
... Secreted hACE2-Fc and mACE2-Fc proteins were purified by protein A chromatography (Goldbio).
-
No products found
because this supplier's products are not listed.
Scott P. Davies, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... supplemented with 10% fetal calf serum (FCS, EuroClone) (complete medium) ...
-
No products found
because this supplier's products are not listed.
Danila Boytsov, et al.,
bioRxiv - Biophysics 2022
Quote:
... the FCS unit (Confocor3, Carl Zeiss, Jena, Germany) of a commercial laser scanning microscope (LSM 510 ...
-
No products found
because this supplier's products are not listed.
Sonali A. Gandhi, et al.,
bioRxiv - Biophysics 2023
Quote:
FCS was performed with an inverted microscope (IX71, Olympus) that was customized to resolve up to four fluorophores as described previously.26 The optical setup included a super-continuum laser (SC-Pro ...
-
No products found
because this supplier's products are not listed.
Daniel Frankel, et al.,
bioRxiv - Biophysics 2020
Quote:
... human (Anaspec) was reconstituted with 1.0% NH4OH and aliquoted before freezing ...
-
No products found
because this supplier's products are not listed.
Kevin P. Foley, et al.,
bioRxiv - Physiology 2020
Quote:
... Human insulin (Mercodia) and human C-peptide (Millipore ...
-
No products found
because this supplier's products are not listed.
Adam K. Wade-Vallance, et al.,
bioRxiv - Immunology 2022
Quote:
... αIgE with a mutated Fc-receptor binding domain (clone R1E4; Cedarlane), or control rat gamma globulin were diluted in D-PBS to a concentration of 0.3mg/mL and injected intravenously to achieve a final dose of 3.25mg/kg ...
-
No products found
because this supplier's products are not listed.
Shivneet K. Gill, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Dilutions of human serum (Human Serum age 4-6, Innovative Research) were performed in TBSM ...
-
No products found
because this supplier's products are not listed.
Trine Lisberg Toft-Bertelsen, et al.,
bioRxiv - Neuroscience 2022
Quote:
... or humans (MBS707296, MyBioSource). The CSF samples were added to wells pre-coated with LPA antibody ...
-
No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... Normal human serum (Complement Technologies) was diluted to 2.3% (v/v ...
-
No products found
because this supplier's products are not listed.
Redouane Aherrahrou, et al.,
bioRxiv - Genetics 2023
Quote:
... Human aortic SMCs (Cell Applications, Inc. ...
-
No products found
because this supplier's products are not listed.
M. Fenu, et al.,
bioRxiv - Biophysics 2023
Quote:
... human fibrinogen (Enzyme Research Laboratories, plasma purified ...
-
No products found
because this supplier's products are not listed.
Erin A. Stephens, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... 50 μM human ubiquitin (Boston Biochem), 4 mM ATP and 1 mM DTT in 20 mM MOPs ...
-
No products found
because this supplier's products are not listed.
M. Sandonà, et al.,
bioRxiv - Cell Biology 2020
Quote:
... anti human CD31-FITC (#21270313, Immunotools), anti human CD45-FITC (#130-080-202 ...
-
No products found
because this supplier's products are not listed.
Xiaoxuan Zhuang, et al.,
bioRxiv - Immunology 2023
Quote:
... containing 10% human serum (Valley Biomedical) and were used within 4 days ...
-
No products found
because this supplier's products are not listed.
Damon A. Hofman, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... human EGF (20ng/mL; Shenandoah Biotech), human FGF-basic-154 (20ng/mL ...