-
No products found
because this supplier's products are not listed.
Cecile Braudeau, et al.,
bioRxiv - Immunology 2020
Quote:
... during 30min and then transferred into a DuraClone IF T Helper cell tube (CD3, CD4, IL-4, IL-17A, IFNγ) (Beckman Coulter), using the IntraPrep fixation permeabilization kit (Beckman Coulter) ...
-
No products found
because this supplier's products are not listed.
Nuria Villalba, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
Primary mouse brain microvascular endothelial cells (Cell Biologics, Chicago, IL; #C57-6023) were grown in the recommended culture medium (Cell Biologics ...
-
No products found
because this supplier's products are not listed.
Brian L. Hie, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
-
No products found
because this supplier's products are not listed.
Brian Belardi, et al.,
bioRxiv - Cell Biology 2019
Quote:
... HEK293 cells were transfected with TransIT-293 (Mirus) according to manufacturer’s instructions with three plasmids ...
-
No products found
because this supplier's products are not listed.
Priyadharshini Devarajan, et al.,
bioRxiv - Immunology 2022
Quote:
... and recombinant Mouse IL-15 (Tonbo Biosciences) were suspended and mixed in PBS ...
-
No products found
because this supplier's products are not listed.
Tania J. Lebratti, et al.,
bioRxiv - Immunology 2020
Quote:
... IL-18 was measured using the mouse IL-18 ELISA kit (MBL International) according to manufacturer’s instructions at half-volumes.
-
No products found
because this supplier's products are not listed.
Zhirong Zhang, et al.,
bioRxiv - Immunology 2021
Quote:
... anti-mouse IL-1β antibody (5129-100, BioVision); anti-mouse Caspase-1 p20 (AG-20B-0042-C100 ...
-
No products found
because this supplier's products are not listed.
Saroj Khadka, et al.,
bioRxiv - Microbiology 2022
Quote:
... or 1:5,000 mouse anti-His-Tag (primary) then 1:5000 goat anti-mouse IgG(H+L)-HRP (secondary) (SouthernBiotech). Blots were developed with ECL Western Blotting Substrate (Pierce ...
-
No products found
because this supplier's products are not listed.
Xi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or plastic-bottom (Ibidi: DLD1, HEK293) 96-well plates at densities targeting 60-70% confluence at the time of transfection ...
-
No products found
because this supplier's products are not listed.
Lisa R. Volpatti, et al.,
bioRxiv - Bioengineering 2024
Quote:
... and rabbit polyclonal anti-mouse IL-10 (1:25, Bioss, bs-0698R) or rabbit polyclonal anti-mouse iNOS (1:50 ...
-
No products found
because this supplier's products are not listed.
Rose Blake, et al.,
bioRxiv - Microbiology 2023
Quote:
... anti-His monoclonal antibody (Raybiotech) and goat anti-rabbit IgG were used as primary and secondary antibodies (Supplementary Table 1) ...
-
No products found
because this supplier's products are not listed.
Richard W. Davis IV, et al.,
bioRxiv - Microbiology 2022
Quote:
... Kanamycin and D-glucose were purchased from Research Products International (RPI; Mt. Prospect, IL). Glycine was purchased from AMERSCO (Solon ...
-
No products found
because this supplier's products are not listed.
Autumn G. York, et al.,
bioRxiv - Immunology 2023
Quote:
IL-10 KO (JAX #002251) and IL-10R KO (JAX #005027) mice were purchased from Jackson labs. Both KO strains were crossed to WT C57BL/6 (JAX 000664 ...
-
No products found
because this supplier's products are not listed.
Kerstin Voelkl, et al.,
bioRxiv - Neuroscience 2023
Quote:
... mouse anti-His (Dianova, DIA-900-100, 1:1,000), chicken anti-EGFP (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Priyabrata Mohanty, et al.,
bioRxiv - Microbiology 2021
Quote:
... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
-
No products found
because this supplier's products are not listed.
Xiaochun Xie, et al.,
bioRxiv - Neuroscience 2022
Quote:
... mouse IL-1α ELISA kit (E-EL-M3059, Elabscience), and moue IFN-β ELISA kit (42410-1 ...
-
No products found
because this supplier's products are not listed.
Xuanwen Li, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Recombinant mouse HEXB protein (His Tag) was obtained from MyBioSource (San Diego, CA). Recombinant proteins alcohol dehydrogenase (ADH ...
-
No products found
because this supplier's products are not listed.
Li Xu, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Total RNA was extracted from mouse tissues and HEK293 cells using Quick-RNA MiniPrep Kit (ZYMO Research, Irvine, CA). Five μg of treated RNA was used as template for first-strand cDNA synthesis by using RevertAid RT Reverse Transcription Kit (Life Technologies ...
-
No products found
because this supplier's products are not listed.
Carolin Fritz, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His (MP Biomedicals) plates ...
-
No products found
because this supplier's products are not listed.
Vishant Boradia, et al.,
bioRxiv - Microbiology 2022
Quote:
... Blots were probed with mouse α-His antibody followed by IRDye® 680RD Goat α-Mouse IgG Secondary Antibody (LI-COR) to detect the His tagged PE15 ...
-
No products found
because this supplier's products are not listed.
Jia Xuan Leong, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
-
No products found
because this supplier's products are not listed.
Carole Seguin-Devaux, et al.,
bioRxiv - Immunology 2024
Quote:
... or rabbit anti-His (Bethyl, ImTec Diagnostic NV ...
-
No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were seeded in 384-well plates (Greiner, #781946) at 30 000 cells/well ...
-
No products found
because this supplier's products are not listed.
Kathryn L. Post, et al.,
bioRxiv - Genetics 2019
Quote:
... Cells were incubated with primary antibodies (1:500 mouse anti-PSD-95 (ABR, IL, USA) or 1:300 mouse anti-gephyrin (Synaptic Systems, Goettingen, Germany) overnight at 4°C in 1% goat serum/PBS ...
-
No products found
because this supplier's products are not listed.
Sarah N. Zvornicanin, et al.,
bioRxiv - Biochemistry 2023
Quote:
HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
-
No products found
because this supplier's products are not listed.
Ichiro Matsuo, et al.,
bioRxiv - Physiology 2021
Quote:
... was purchased from Thermo Fisher (Rockland, IL, USA). The primary antibodies against p62 (1:1000 ...
-
No products found
Wei Dong, et al.,
bioRxiv - Cell Biology 2020
Quote:
HEK293 cells were cultured in glass bottom dishes (In Vitro Scientific) and were transfected with DNA using X-treme Gene 9 DNA transfection reagent (Sigma Cat# 6365787001) ...
-
No products found
because this supplier's products are not listed.
Elisa Tonoli, et al.,
bioRxiv - Neuroscience 2021
Quote:
... primary astrocytes were obtained from BrainBits (BrainBits, Springfield, IL, USA) and processed following the manufacturer’s protocol.
-
No products found
because this supplier's products are not listed.
Pablo Alcón, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ...
-
No products found
because this supplier's products are not listed.
Xinrui Wang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His-tone H2A.Z (1:200, Active Motif #39013), Nkx2.5 (1:250 ...
-
No products found
because this supplier's products are not listed.
J-H Schroeder, et al.,
bioRxiv - Immunology 2020
Quote:
... IL-27Rα KO (Charles River) and Ert2 (Jackson labs ...
-
No products found
because this supplier's products are not listed.
Serena Cortés-Kaplan, et al.,
bioRxiv - Immunology 2021
Quote:
... APC counting beads (Spherotech, IL) were added ...
-
No products found
because this supplier's products are not listed.
Weisong Zhao, et al.,
bioRxiv - Biophysics 2022
Quote:
... equipped with an objective (100×/1.7 HI oil, APON, Olympus)and a sCMOS (Flash 4.0 V3 ...
-
No products found
because this supplier's products are not listed.
Katherine Wallis, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... L-homocysteine (Chem-impex, Wood Dale, IL) was resuspended in PBS with NaOH added until complete solubilization ...
-
No products found
because this supplier's products are not listed.
Ge Song, et al.,
bioRxiv - Immunology 2020
Quote:
... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
-
No products found
because this supplier's products are not listed.
Elmira Alipour, et al.,
bioRxiv - Pathology 2023
Quote:
A Masterflex pump (Cole-Parmer, Vernon Hills, IL) flowed nitrogen gas through empty circuits for 20 minutes ...
-
No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
-
No products found
because this supplier's products are not listed.
Fan Liu, et al.,
bioRxiv - Neuroscience 2022
Quote:
... rabbit anti-IL-18 (1:100, Cat: PAB16177, Abnova), rabbit anti-IL-1β (1:100 ...
-
No products found
because this supplier's products are not listed.
Paul J. Baker, et al.,
bioRxiv - Immunology 2024
Quote:
... pro-IL-1β was detected using goat-α-IL-1β (R&D, #AB-401-NA, 1:1500) and bovine-α-goat-HRP (Jackson ImmunoResearch, #805-035-180, 1:10,000) and actin was detected using mouse-α-actin-HRP (Santa Cruz ...
-
No products found
because this supplier's products are not listed.
Cathal Meehan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Recombinant IL-6 with an N-terminal his tag (Fitzgerald, Biosynth Ltd.) was immobilized on His-Pur™ Ni-NTA Resin (ThermoScientific ...
-
No products found
because this supplier's products are not listed.
Arun Prasath Damodaran, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... mouse anti-His tag (HIS.H8 / EH158, Covalab, 1:2500), mouse anti-FLAG tag (clone M2-F1804 ...
-
No products found
because this supplier's products are not listed.
Andrea M. Chambers, et al.,
bioRxiv - Immunology 2021
Quote:
... 1 µg/mouse of IL-15 (Shenandoah Biotech) was injected three times per week with an intraperitoneal injection (ip) ...
-
No products found
because this supplier's products are not listed.
Danny Galleguillos, et al.,
bioRxiv - Neuroscience 2020
Quote:
... recombinant mouse IL-1β was purchased from Cedarlane (Cedarlane CLCYT273), L-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol•HCl (L-t-PDMP ...
-
No products found
because this supplier's products are not listed.
AL Chenery, et al.,
bioRxiv - Immunology 2020
Quote:
... fusion proteins were generated of mouse IL-4 and IL-13 with the Fc portion of IgG1 (custom order with Absolute Antibody). Mice were injected intraperitoneally with either PBS ...
-
No products found
Kirandeep K. Deol, et al.,
bioRxiv - Biochemistry 2020
Quote:
HEK293 Expressing Rpn11-HTBH (Applied Biological Materials, T6007)
-
No products found
because this supplier's products are not listed.
Lyra O. Randzavola, et al.,
bioRxiv - Immunology 2022
Quote:
... HEK293-F were transfected with polyethylenimine (Polyscience Europe GmbH) at a ratio of 1:3 DNA:polyethylenimine (Tom et al. ...
-
No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a microplate reader (BioTek Synergy HI) to record the absorbance at 650 nm ...
-
No products found
because this supplier's products are not listed.
Chongyang Li, et al.,
bioRxiv - Cancer Biology 2019
Quote:
The bacterial expression vectors pReceiver-B11 for His-NSMCE2 and His-PFDN2 recombinant protein expression were purchased from Genecopoeia, Inc (Rockville ...
-
No products found
because this supplier's products are not listed.
Emilia Neuwirt, et al.,
bioRxiv - Immunology 2022
Quote:
... Cell viability was assessed as in the primary screen and IL-1β in the cell- free supernatants was detected using a commercial mouse IL-1β homogenous time-resolved fluorescence (HTRF) kit (Cisbio) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Ashley A. Johnson, et al.,
bioRxiv - Physiology 2021
Quote:
... Spectra were measured from HEK293 cells using a 60x objective with NA 1.45 (Nikon) and an inverted microscope (Nikon TE-2000) ...