-
No products found
because this supplier's products are not listed.
K. Polak, et al.,
bioRxiv - Immunology 2020
Quote:
... or S2 domain (SARS-CoV-2 Spike S2, mouse monoclonal antibody [1A9], GTX632604, Genetex), or CilPP (in-house antibody raised in rabbit) ...
-
No products found
because this supplier's products are not listed.
Esther A. Mozipo, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Recombinant human bone morphogenetic protein-2 (BMP-2) and the human BMP-2 DuoSet enzyme-linked immunosorbent assay (ELISA ...
-
No products found
because this supplier's products are not listed.
Lih-Yun Hsu, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Human IL-2 concentration was measured with the AlphaLISA human IL2 Kit (PerkinElmer) and data collected on an Envision Plate Reader (PerkinElmer) ...
-
No products found
because this supplier's products are not listed.
Yinglin Ji, et al.,
bioRxiv - Plant Biology 2020
Quote:
... Purified PuACO1-GST protein (0.2 μg) was added to 2 ml of incubation buffer (pH 7.2) containing 10% (v/v) glycerol (Solarbio, http://www.solarbio.com), 5 mM Na-ascorbate (Sangon Biotech) ...
-
No products found
because this supplier's products are not listed.
Luyu Qi, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
The cDNA of human HMGB1 domain A (HMGB1a, Supplementary Chart 1) was synthesized by Proteintech (Rosemont, USA) and cloned into pET-28a plasmid ...
-
No products found
because this supplier's products are not listed.
Yihong Chen, et al.,
bioRxiv - Cell Biology 2023
Quote:
... NRP-1 b1b2 domains were labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies, Munich, Germany). The interaction of NRP-1 structural variants with FOL-peptides was measured using a Monolith NT.115 [NT.115Pico/NT.LabelFree] instrument (NanoTemper Technologies ...
-
No products found
because this supplier's products are not listed.
Nathan Jariwala, et al.,
bioRxiv - Cell Biology 2023
Quote:
... were cultured in HDF growth media supplemented with FBS (2% v/v) basic fibroblast growth factor (recombinant human; 1ng/ml) and insulin (5 μg/ml) (all from PromoCell). All cells were grown in a humidified environment at 37°C with 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Douglas C. Palmer, et al.,
bioRxiv - Immunology 2020
Quote:
... the cells were then cultured in AIM-V medium containing 10% human AB serum (Valley Biomedical) and 300□IU/mL IL-2 until use.
-
No products found
because this supplier's products are not listed.
Kok-Siong Chen, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... sections were incubated for 2 h in blocking solution containing 10% (v/v) normal donkey or goat serum (Jackson Laboratories, Bar Harbor, Maine), 0.9% (v/v ...
-
No products found
because this supplier's products are not listed.
Jui-Hsien Chang, et al.,
bioRxiv - Neuroscience 2020
Quote:
... AFL was labeled at the Fc portion of human IgG immunoglobulin (goat polyclonal anti-human IgG Fc antibody, NB7446, Novus Biologicals, 1:200 at 4C overnight). Stainings were followed by a corresponding Alexa Fluor-conjugated secondary antibody (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Sílvia Vale-Costa, et al.,
bioRxiv - Cell Biology 2023
Quote:
Cells grown on 3 mm aclar disks (carbon coated) were fixed using a mixture of 2% (v/v) formaldehyde and 0.2% (v/v) glutaraldehyde (Polysciences) in 0.1M phosphate buffer ...
-
No products found
because this supplier's products are not listed.
Farizeh Aalam, et al.,
bioRxiv - Immunology 2020
Quote:
Infections were performed as described above except KSHV.219 virus was pre-incubated for 30 minutes on ice with serum free RPMI only or serum free RPMI containing recombinant human syndecan-1 protein (rhCD138, BioVision, 7879-10) prior to being added to Naïve B lymphocytes ...
-
No products found
because this supplier's products are not listed.
Daniel J. Steiner, et al.,
bioRxiv - Immunology 2020
Quote:
... pooled normal human serum (PNHS; 10% v/v, Innovative Research) was used to dilute monoclonal and polyclonal antibodies as well as donor human serum samples to appropriate standard concentrations ...
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No products found
because this supplier's products are not listed.
Madeleine F. Jennewein, et al.,
bioRxiv - Immunology 2023
Quote:
... purified spike ectodomain trimers and RBD domain protein were biotinylated by the addition of biotin-protein ligase (Avidity). Biotinylated proteins were buffer exchanged into PBS ...
-
No products found
because this supplier's products are not listed.
Naomine Yano, et al.,
bioRxiv - Biophysics 2022
Quote:
... 10% [v/v] 2-propanol and 30% [w/v] PEG1500) that was purchased from Hampton research. Because the crystals did not grow in homemade crystallization solutions (21) ...
-
No products found
because this supplier's products are not listed.
Sonali Jindal, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... human COX2 protein (Cayman Chemical, 60122, 4ng), and 25μg cell line lysates in RIPA buffer were separated by WES automated gel electrophoresis System (Protein Simple ...
-
No products found
because this supplier's products are not listed.
Mukund Madhav, et al.,
bioRxiv - Microbiology 2019
Quote:
... A grid (2 cm X 2 cm) was set up on the camera screen (Nikon, Sydney, Australia) for cell counting ...
-
No products found
because this supplier's products are not listed.
Catarina Nascimento, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and after two washing steps with PBS the nonspecific binding of immunoglobulins was prevented by incubating the tissue slices with the Protein Block Novocastra Solution (Leica Biosystems) for 60 min ...
-
No products found
because this supplier's products are not listed.
Jacob K. Sterling, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 2% Triton X-100 in PBS) and brain-specific homeobox/POU domain protein-3a (Brn3a; Synaptic Systems, Goettingen, Germany) diluted 1:1000 in blocking buffer ...
-
No products found
because this supplier's products are not listed.
Seunghee Oh, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Recombinant Snf1 kinase domain (Snf1-KD) was phosphorylated by recombinant human CaMKK2 (Abnova) for its activation before kinase assays (Figure 5F) ...
-
No products found
because this supplier's products are not listed.
Johanna Krüger, Holger Lerche,
bioRxiv - Neuroscience 2023
Quote:
... containing 10% (v/v) fetal calf serum (FCS; PAN Biotech) and were cultured at 37°C in a 5 % CO2 humidified atmosphere ...
-
No products found
because this supplier's products are not listed.
Shajo Kunnath-Velayudhan, et al.,
bioRxiv - Immunology 2021
Quote:
... immunoglobulin-depleted FBS (10%; Atlanta Biologicals), β-mercaptoethanol (Life Technologies) ...
-
No products found
because this supplier's products are not listed.
Shuhei Aihara, et al.,
bioRxiv - Neuroscience 2020
Quote:
... The chamber was set under a 2-photon microscope (Olympus, FV1000MPE) equipped with a water-immersion 25x objective lens (Olympus ...
-
No products found
because this supplier's products are not listed.
David J Vance, et al.,
bioRxiv - Immunology 2024
Quote:
... Biotinylated OspA (5 µg/mL) in PBS containing 2% w/v BSA was captured onto streptavidin biosensors (#18-5019, Sartorius) for 5 min ...
-
No products found
because this supplier's products are not listed.
Kenta Mosallanejad, et al.,
bioRxiv - Immunology 2022
Quote:
... and rat immunoglobulins (IgGs) were purchased from Rockland Immunochemicals ...
-
No products found
because this supplier's products are not listed.
Koray Malcı, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... and 2% (w/v) glucose (Alfa Aesar™) or 2% (w/v ...
-
No products found
because this supplier's products are not listed.
Loreleï Boyer, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... RNA extraction was performed using the “NucleoSpin RNA Set for NucleoZOL” kit (Macherey-Nagel), following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Yingying Chen, et al.,
bioRxiv - Neuroscience 2023
Quote:
... rabbit anti-immunoglobulin like domain containing receptor 1 (ILDR1) (Antibodies-online.com, 1:200, Cat # ABIN1386369), (6 ...
-
No products found
because this supplier's products are not listed.
Junghyun L. Suh, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... A comprehensive library of human protein domains that potentially read methyllysine marks was cloned into a pGEX vector by Biomatik (Cambridge, Canada) using gene synthesis to best optimize the open reading frames for bacterial expression ...
-
No products found
because this supplier's products are not listed.
Enrica Saponara, et al.,
bioRxiv - Physiology 2021
Quote:
... A volume of 800 μL of chloroform:methanol (2:1 v/v) containing 15 μL internal standards (ISTDs, EquiSPLASH, Avanti Polar Lipids) were homogenized using the bead beating device (Precellys ...
-
No products found
because this supplier's products are not listed.
Zihao Wang, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... The crystal for structural determination of HSPB7ACD C131S was discovered and harvested after setting up for 10 days from a well containing 0.2 M magnesium formate dihydrate and 20% w/v polyethylene glycol (PEG) 3350 (JCSG-plus A5, Molecular Dimensions, protein: precipitant ratio 2:1). Crystals were cryoprotected using 10% glycerol in well solution ...
-
No products found
because this supplier's products are not listed.
Yan Luo, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... 2% v/v Gem21 NeuroPlex™ Serum-Free (without Vitamin A) (Gemini Bio-Products, USA), FGF (20 ng/ml PeproTech ...
-
No products found
because this supplier's products are not listed.
Quentin Defenouillere, et al.,
bioRxiv - Cell Biology 2019
Quote:
... The 2-NBDG signal was visualized using a GFP filter set (41020 from Chroma Technology, Bellows Falls ...
-
No products found
because this supplier's products are not listed.
Shucheng Chen, Mark Harris,
bioRxiv - Microbiology 2022
Quote:
... Proteins were transferred to polyvinylidene fluoride (PVDF) membrane and blocked with 50% (v/v) Odyssey blocking buffer (LI-COR) diluted in 1X Tris-buffered saline with Tween-20 (TBS-T ...
-
No products found
because this supplier's products are not listed.
Anna Marie Hugon, Thaddeus G. Golos,
bioRxiv - Microbiology 2023
Quote:
Human Caco-2 cells (HTB37; American Type Culture Collection) purchased from ATCC (Manassas ...
-
No products found
because this supplier's products are not listed.
Stefan Dannenmaier, et al.,
bioRxiv - Cell Biology 2021
Quote:
... resuspended in 20 µl of PBS containing 0.1% (v/v) Triton X-100 and analyzed using a Zeiss LSM 880 (Carl Zeiss AG, Oberkochen, Germany) equipped with a W-Plan Apochromat 63x/1.0 water objective and an Airyscan detection unit ...
-
No products found
because this supplier's products are not listed.
Bryce W. Duncan, et al.,
bioRxiv - Neuroscience 2021
Quote:
... coiled-coil containing protein kinase (ROCK1/2) Y27632 (Tocris, Inc.) at 10 µM in PBS for 1-2 hr ...
-
No products found
because this supplier's products are not listed.
Kai Xia, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Testosterone levels were measured using a chemiluminescent immunoassay (CLIA) system (Architect system; Abbott GmbH & Co. KG, Germany). The coefficient of variation of this CLIA system is 1.9–5.1% for intra-assay precision and 2.5–5.2% for inter- assay precision ...
-
No products found
because this supplier's products are not listed.
Rachel J. Harding, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... 2% DMSO (v/v) in a 384-well microplate (Greiner). A final concentration of 50 µM Boc-Lys(TFA)-AMC was added and incubated for 1 hour at room temperature followed by the addition of developer solution containing a final concentration of 50 µM TSA ...
-
No products found
because this supplier's products are not listed.
Katharina Sydow, et al.,
bioRxiv - Microbiology 2024
Quote:
... v.2 (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions and quantified with the Qubit 4 fluorometer and the dsDNA HS Assay kit (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
John C. Obenauer, et al.,
bioRxiv - Neuroscience 2023
Quote:
The proteomics signature had 4 validation data sets: R6/2 mice aged 2 months (R6/2 2M) or 3 months (R6/2 3M) from PRIDE experiment PXD013771 ...
-
No products found
because this supplier's products are not listed.
Dipti Vinayak Vernekar, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The extension products were analysed on 1% agarose gel containing GelRed (1:10,000 v/v, Biotium) in TAE buffer ...
-
No products found
because this supplier's products are not listed.
Nguyen-Vi Mohamed, et al.,
bioRxiv - Neuroscience 2021
Quote:
... voltages were set according to optimal PMT sensitivity using the peak 2 (Spherotech) voltration technique described previously by Maeker and Trotter 35 ...
-
No products found
because this supplier's products are not listed.
Stephanie K. See, et al.,
bioRxiv - Cell Biology 2021
Quote:
... qPCR reactions were set up using SensiFast 2× Mastermix (Bioline Cat# BIO-94020) and oligonucleotides targeting genes of interest (IDT ...
-
No products found
because this supplier's products are not listed.
Laurent Acquaviva, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... 15 μl of hybridization mix was applied containing the DNA probe(s) in 70% (v/v) deionized formamide (Amresco), 10% (w/v ...
-
No products found
because this supplier's products are not listed.
Rabea Marie Wagner, et al.,
bioRxiv - Microbiology 2020
Quote:
... Data were acquired using ABi 4000 Series Explorer Spot Set Manager software and processed using ABi 4000 Series Explorer Software v.3.6 (both SCIEX). The detected peptides were compared against the genome of B ...
-
No products found
because this supplier's products are not listed.
J. Sevilla-Montero, et al.,
bioRxiv - Cell Biology 2022
Quote:
... containing 2% fetal bovine serum (FBS, ScienCell, #0010), 1X smooth muscle cell growth supplement (ScienCell ...
-
No products found
because this supplier's products are not listed.
Carolina Rosselot, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Plasma human insulin was measured using the human insulin ELISA kit (Mercodia, Winston Salem, NC) and plasma human glucagon was measured by the human glucagon ELISA kit (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
Jiechao Zhou, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and purified human C1q protein (2 µg/mL, Complement Technology) was suspended in GVB++ Buffer (Complement Technology ...
-
No products found
because this supplier's products are not listed.
Sarah Ennis, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... 2.5 mM CaCl2) containing 1.5 µl Annexin V-APC (ImmunoTools). The cells were incubated on ice in the dark for 15 min ...