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No products found
because this supplier's products are not listed.
Katarzyna Bogucka-Janczi, et al.,
bioRxiv - Cell Biology 2022
Quote:
shRNAs plasmids were purified from the MISSION® shRNA Human Library (Sigma). Non-targeting control shRNA (shCo ...
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No products found
because this supplier's products are not listed.
Fu-Qing Jiang, et al.,
bioRxiv - Cell Biology 2022
Quote:
The full-length Mettl3 coding sequences were amplified from the pcDNA3-Mettl3 plasmid (Addgene, #53739), and cloned into AAV serotype-9 expressing plasmid to overexpress Mettl3 (AAV9-Mettl3) ...
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No products found
because this supplier's products are not listed.
Anjali Balakrishnan, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... we used NSMase2 (SMPD3) Human shRNA Plasmid Kit (Origene, Locus ID 55512), which included what we termed shSMPD3 variants A-D and shScr ...
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No products found
because this supplier's products are not listed.
Matthew T. Sacco, et al.,
bioRxiv - Microbiology 2022
Quote:
... METTL3 (Abcam, ab195352 ...
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No products found
because this supplier's products are not listed.
Jun Yang, et al.,
bioRxiv - Cell Biology 2023
Quote:
COL11A1 shRNA plasmid was obtained from NIEHS Genomic core (Thermo Scientific GIPZ Lentiviral shRNA). Lentiviral packaging was produced at the NIEHS Viral Vector Core ...
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No products found
because this supplier's products are not listed.
Zhongyu Zou, et al.,
bioRxiv - Cell Biology 2022
Quote:
... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757, human YTHDF3: QIAGEN SI04133339, human METTL3: QIAGEN SI05020414 and human DDX6 ...
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No products found
because this supplier's products are not listed.
Anupriya Aggarwal, et al.,
bioRxiv - Microbiology 2021
Quote:
... shRNA plasmids (pool of three shRNA plasmids per target) were obtained directly from Santa Cruz.
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No products found
because this supplier's products are not listed.
Lior Lasman, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Mettl3 (Proteintech Group 15073-1-AP), Nanog (Bethyl ...
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No products found
because this supplier's products are not listed.
Shreya Budhiraja, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... shRNA plasmids were procured from Genecopoeia (Rockville, MD, USA), and overexpression plasmids were obtained from AddGene (Watertown ...
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No products found
because this supplier's products are not listed.
Rohtem Aviram, et al.,
bioRxiv - Cell Biology 2024
Quote:
... MISSION® shRNA lentiviral plasmids (Merck) were used for knockdown of LOX (shLOX1 – TRCN0000045991 ...
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No products found
because this supplier's products are not listed.
Adam G Grieve, et al.,
bioRxiv - Biochemistry 2020
Quote:
... in 35mm plates with 0.5 μg of pLKO shRNA or pLVX expression plasmids (Takara), 0.35 μg pCMV-dR8.2 and 0.15 μg pCMV-VSVG ...
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No products found
because this supplier's products are not listed.
Natalia G. Sampaio, et al.,
bioRxiv - Immunology 2021
Quote:
... and 1 μg shRNA plasmid using Fugene 6 (Promega). The next day ...
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No products found
because this supplier's products are not listed.
Eliza S. Lee, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... METTL3 (ABclonal, A8370), YTHDC1 (Abcam ...
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No products found
because this supplier's products are not listed.
Danielle L. Tomasello, et al.,
bioRxiv - Neuroscience 2021
Quote:
... DYKDDDDK (Flag)-tagged human FAM57B plasmid was purchased from Genscript. Hemagglutinin (HA)-tagged human CerS plasmids were generated as described76.
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No products found
because this supplier's products are not listed.
Lisa Baker, et al.,
bioRxiv - Neuroscience 2020
Quote:
... except cells were transduced with AAV5 containing a control scrambled shRNA plasmid or a FL2 shRNA plasmid (Vector Biolabs). shRNA sequences were cloned into a D2-U6 vector ...
-
No products found
because this supplier's products are not listed.
Changhe Ji,
bioRxiv - Neuroscience 2022
Quote:
shRNAs targeting human HNRNPR were cloned into a modified version of pSIH-H1 shRNA vector (System Biosciences) containing EGFP according to the manufacturer’s instructions ...
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No products found
Md Imamul Islam, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Scramble (LV-shRNA-scramble, #LVP015-G) and Trx1 shRNA plasmids (LV-shRNA-Trx1, #488660940395) were purchased from Applied Biological Materials Inc. ...
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No products found
because this supplier's products are not listed.
Xizi Chen, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Lentivirus expressing short-hairpin RNAs was prepared by transfecting PLKO.1 shRNA plasmids and packaging plasmids containing psPAX2 and pMD2.G into 293T cells using PEI (Polysciences). Collected conditional media containing virus particles were used to transduce cells in the growth media supplemented with Polybrene for 24 hours ...
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No products found
because this supplier's products are not listed.
Ratnadeep Mukherjee, et al.,
bioRxiv - Immunology 2020
Quote:
... 5 µg of NOSIP shRNA plasmids or control TRC2 plasmid was mixed with 100 µl Cell Line Nuceleofector Solution V (Lonza) and added to one million cells ...
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No products found
because this supplier's products are not listed.
Shiyi Wang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... a DNA fragment containing hU6 and shRNA was amplified from pLKO.1-shRNA using Phusion High-Fidelity DNA Polymerase (NEB) with primers that introduced SpeI restriction sites (Forward Primer ...
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No products found
because this supplier's products are not listed.
Dario F. De Jesus, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... vector pcDNA3/Flag-METTL3 (addgene #53739) was transformed and the plasmid was extracted with Axygen kit (Corning). Next ...
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No products found
because this supplier's products are not listed.
Valerio Taverniti, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... 38HK were transiently transfected with lentiviral shRNA plasmids using TransIT-Keratinocytes Transfection Reagent (Mirus) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Duc T. Tran, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Plasmids expressing human PRDX4-FLAG and Human QSOX1-HA were purchased from Sino Biological Inc ...
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No products found
because this supplier's products are not listed.
Matthew T. Sacco, et al.,
bioRxiv - Microbiology 2022
Quote:
... METTL3 (Novus Biologicals, AB_2687437; Immunoblot), METTL3 (Abcam ...
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No products found
because this supplier's products are not listed.
Sanghee Yun, et al.,
bioRxiv - Animal Behavior and Cognition 2023
Quote:
... Each annealed oligonucleotide was ligated into the adeno-associated virus (AAV2) plasmid (pAAV-EGFP-shRNA; Stratagene, La Jolla, CA)(Hommel et al. ...
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No products found
because this supplier's products are not listed.
Jonathan Bayerl, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... METTL3 (A301-567A, 1:2000, Bethyl), HSP90beta (ab32568 ...
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No products found
because this supplier's products are not listed.
Janet F. Staab, et al.,
bioRxiv - Cell Biology 2020
Quote:
Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...
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No products found
because this supplier's products are not listed.
Ulli Heydasch, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Transfection of plasmids for knockdown purposes (shRNA-vectors) was performed using FuGene®HD (Roche Diagnostics) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Amalie C. Grenov, et al.,
bioRxiv - Immunology 2020
Quote:
... Intracellular antibody staining for METTL3 was performed after fixation and permeabilization with Fixation/Permeabilization Solution Kit (BD biosciences).
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No products found
because this supplier's products are not listed.
Ning Yang, et al.,
bioRxiv - Microbiology 2021
Quote:
... Human cGAS (hcGAS) and murine cGAS (mcGAS) plasmids were purchased from Invivogen. Flag-cGAS and V5-cGAS were amplified by PCR and were cloned into pcDNA3.2-DEST plasmids ...
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No products found
because this supplier's products are not listed.
Michael J. McFadden, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... mouse anti-METTL3 (Abnova H00056339-B01P, 1:1000), rabbit anti-FTO (Abcam EPR6895 ...
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No products found
because this supplier's products are not listed.
Francesca Puca, et al.,
bioRxiv - Cancer Biology 2020
Quote:
The inducible shRNA was obtained from Cellecta by cloning into a pRSIT16-U6Tet-sh-CMV-TetRep-2A-TagRFP-2A-Puro vector the following hairpin sequence:
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No products found
because this supplier's products are not listed.
Kellie A. Cotter, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Blots were blocked for 1 hour at room temperature and then probed with primary antibodies against METTL3 (Cell Signaling, 96391), KIF5C (Abcam ...
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No products found
because this supplier's products are not listed.
Meriem Belabed, et al.,
bioRxiv - Immunology 2023
Quote:
Human: Anti-human CD141 (clone M80, BioLegend), anti-human CD45RA (clone HI100 ...
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No products found
because this supplier's products are not listed.
Nataly P. Podolnikova, et al.,
bioRxiv - Cell Biology 2022
Quote:
The cDNA encoding the extracellular N-terminal IgV domain of human CD47 (residues 19-141) was amplified from the plasmid hCD47 VersaClone cDNA (RDC1523, R&D systems) and was cloned in the expression vector pGEX-4T-1 (GE Healthcare) ...
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No products found
because this supplier's products are not listed.
Florent Peglion, et al.,
bioRxiv - Cell Biology 2021
Quote:
... human FGF-basic and human EGF (animal free, Peprotech) at 20 ng/mL ...
-
No products found
because this supplier's products are not listed.
Nitin Raj, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Flp-In system compatible N-terminally FLAG- tagged Mettl3 (Flag-Mettl3) was generated by LR recombination between Mettl3 entry vector and pG-LAP2/puro destination vector (gift from Peter Jackson lab, Stanford University). Q5 site-directed mutagenesis (NEB E0554S ...
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No products found
because this supplier's products are not listed.
Joachim M. Surm, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
... shRNAs were synthesized with AmpliScribe T7-Flash Transcription Kit (Epicentre, Charlotte, NC) for 15 h followed by 15 min treatment with 1 µl of DNase I ...
-
No products found
because this supplier's products are not listed.
Ali Akbar Karkhaneh Yousefi, et al.,
bioRxiv - Biophysics 2023
Quote:
... using 10% Human Fn (Human Fn, Promocell) in PBS ...
-
No products found
because this supplier's products are not listed.
Gonzalo Quiroga-Artigas, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Newly synthesized shRNAs were then purified using the Direct-zol™ RNA MiniPrep Kit (Zymo Research), eluted in 35μl of nuclease-free H2O and quantified with a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Valeriya Malysheva, et al.,
bioRxiv - Genetics 2022
Quote:
... anti-human-CD19 and anti-human-CD14 (Miltenyi Biotec) and transferred through LD columns (Miltenyi Biotec ...
-
No products found
because this supplier's products are not listed.
Gemma E. Hartley, et al.,
bioRxiv - Immunology 2023
Quote:
... Serially diluted recombinant human IgG1 or human IgG4 (BioRad, Hercules, CA) with unrelated specificities were used for quantification in separate wells on the same plate.
-
No products found
because this supplier's products are not listed.
Julia Prigann, et al.,
bioRxiv - Microbiology 2023
Quote:
... Human NK cell or Human Monocyte CD14+ Isolation kits (STEMCELL Technologies), respectively ...
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No products found
because this supplier's products are not listed.
D. Czernecki, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... plasmids were isolated with NucleoSpin Plasmid QuickPure kit (Macherey-Nagel) and suspended in water ...
-
No products found
because this supplier's products are not listed.
Qing Zhao, et al.,
bioRxiv - Immunology 2023
Quote:
Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01; anti-human IgA, SouthernBiotech, Cat # 2052-01 ...
-
No products found
because this supplier's products are not listed.
Shu Liu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Human primary astrocytes (ScienCell) were cultivated as recommended by ScienCell ...
-
No products found
because this supplier's products are not listed.
Li-Ying Yu, et al.,
bioRxiv - Neuroscience 2023
Quote:
METTL3/14 complex (26342, Cayman Chemical) was labelled via His-tag with Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies GmbH) ...
-
No products found
because this supplier's products are not listed.
Huanhuan Joyce Chen, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The lentiviral vector expressing tetracycline (TET)-inducible shRNA against human P53 was purchased from Gentarget. Inc ...
-
No products found
because this supplier's products are not listed.
Anthony A. Ruberto, et al.,
bioRxiv - Genomics 2021
Quote:
... primary human hepatocytes (BioIVT) were seeded 2-3 days prior to infection with 15,000-20,000 sporozoites per well ...
-
No products found
because this supplier's products are not listed.
Andrew J Kinloch, et al.,
bioRxiv - Immunology 2020
Quote:
... Alexa Fluor 488 goat anti-human IgG and anti-human IgG HRP (Jackson ImmunoResearch).