-
No products found
because this supplier's products are not listed.
Shiyu Sun, et al.,
bioRxiv - Immunology 2021
Quote:
... human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the SARS-VoV-2 S protein were co-transfected into 293T cells (ATCC) ...
-
No products found
because this supplier's products are not listed.
Fatima Alghoul, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and 1 unit/μl Avian Myoblastosis virus reverse transcriptase (Promega®) were added and incubated for 1h at 30°C ...
-
No products found
because this supplier's products are not listed.
Mesfin Meshesha, et al.,
bioRxiv - Microbiology 2020
Quote:
... except that HIV-1 reverse transcriptase (RTase) was used in the extension assay (Millipore-Sigma). Further details are described in the S1 Supplemental Methods.
-
No products found
because this supplier's products are not listed.
Ross D Overacker, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
Antiviral azaBINOL B#24 was assessed for inhibition of HIV-1 reverse transcriptase using a commercially available colorimetric reverse transcriptase assay kit (Roche) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Donghwi Bae, et al.,
bioRxiv - Cell Biology 2022
Quote:
... and Moloney murine leukemia virus reverse transcriptase (New England Biolabs). Using a QuantStudio 3 real-time quantitative PCR machine (Life Technologies) ...
-
No products found
because this supplier's products are not listed.
Bin Zhang, et al.,
bioRxiv - Immunology 2020
Quote:
... and total RNA was reverse-transcribed to cDNA with Moloney Murine Leukemia Virus Reverse Transcriptase (2641B, TAKARA). Real-time quantitative PCR (qPCR ...
-
No products found
because this supplier's products are not listed.
George D. Moschonas, et al.,
bioRxiv - Microbiology 2023
Quote:
... mouse monoclonal anti-Human Immunodeficiency Virus Type 1 (HIV-1) p24 clone [39/5.4A] (#ab9071, Abcam, dilution 1:500). Mouse monoclonal anti-Human Immunodeficiency Virus Type 1 (HIV-1 ...
-
No products found
because this supplier's products are not listed.
Flavia Scoyni, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... or LV1-eGFP-miR-7 were generated by subcloning inserts from pAAV_hSYN1-eGFP-miR-7 and pAAV_hSYN1-eGFP (provided by Thomas B. Hansen) inside LV1 (immunodeficiency virus 1 (HIV-1)-based LV-PGK-GFP) backbone by GenScript Biotech Corporation ...
-
No products found
because this supplier's products are not listed.
Alejandro L. Antonia, et al.,
bioRxiv - Microbiology 2021
Quote:
... Reverse transcriptase was performed using iScript Reverse Transcriptase kit (BioRad, 1708840). Quantitative real-time PCR (qRT-PCR ...
-
No products found
because this supplier's products are not listed.
Chun Hao Wong, et al.,
bioRxiv - Cell Biology 2023
Quote:
... QuantiTect reverse transcriptase (Qiagen) was used for cDNA synthesis with an input of 800 ng RNA per sample ...
-
No products found
because this supplier's products are not listed.
Anh T. Nguyen, et al.,
bioRxiv - Microbiology 2020
Quote:
... MMLV reverse transcriptase (Agilent) at 42° C for 45 min.
-
No products found
because this supplier's products are not listed.
Liguo Zhang, et al.,
bioRxiv - Genomics 2020
Quote:
Plasmid for HIV-1 reverse transcriptase expression: pCMV-dR8.2 dvpr was a gift from Bob Weinberg (Addgene plasmid # 8455 ...
-
No products found
because this supplier's products are not listed.
Evan S. Bardot, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... anti-human CD172a/b (SIRPα/β) (Biolegend; clone SE5A5; 1:200) anti-human CD13 (Biolegend ...
-
No products found
because this supplier's products are not listed.
Laura Brueckner, et al.,
bioRxiv - Genomics 2019
Quote:
... RNA was reverse-transcribed using Tetro Reverse Transcriptase (Bioline) with Oligo(dT)20 primers (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Lana Langendries, et al.,
bioRxiv - Microbiology 2022
Quote:
... 0.0625 µl reverse transcriptase (Eurogentec) and 3 µl RNA sample ...
-
No products found
because this supplier's products are not listed.
Mona Rheinberger, et al.,
bioRxiv - Genomics 2022
Quote:
... HIV-1 p24 was stained with Anti-HIV-1 p24-PE (KC57) (Beckman Coulter) for 30 min on ice and washed again 3 times before measuring on the BD FACSCelesta™.
-
No products found
because this supplier's products are not listed.
Catherine M. Card, et al.,
bioRxiv - Immunology 2020
Quote:
Culture supernatants were diluted 20-fold with PBS + 1% BSA + 0.2% Triton X-100 then analyzed for HIV-p24 protein using the HIV-1 Gag p24 DuoSet ELISA Development System (R&D Systems) with the R&D TMB substrate reagent pack for detection ...
-
No products found
because this supplier's products are not listed.
Tho-Alfakar Al-Aubodah, et al.,
bioRxiv - Immunology 2023
Quote:
... anti-human CD21 BV421 (1:20, B-ly4, BD Biosciences), anti-human CD27 PE-Cy7 (1:20 ...
-
No products found
because this supplier's products are not listed.
Scott A. Rush, et al.,
bioRxiv - Immunology 2022
Quote:
B cells were enriched from human PBMCs using EasySep Human Pan-B Cell Enrichment Kit (StemCell Technologies) before being incubated with Fc block (BD Biosciences ...
-
No products found
because this supplier's products are not listed.
Chuan Xu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Virus stocks were analyzed for HIV-1 p24 antigen by the AlphaLISA HIV p24 kit (PerkinElmer). Virus stocks contained approximate 200 ng/ml of HIV p24 protein.
-
No products found
because this supplier's products are not listed.
John A. Frank, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Transfected cells were infected with reporter virus by applying virus (HIV-RD114env, HIV-VSVg, HIV-SMRVenv) stocks in the presence of polybrene (Santa Cruz Bio, sc-134220) at a final concentration of 4 ug/mL ...
-
No products found
because this supplier's products are not listed.
Matheus F. Sathler, et al.,
bioRxiv - Neuroscience 2022
Quote:
... NIH: HIV-1 IIIB gp120 Recombinant Protein from ImmunoDX, LLC ...
-
No products found
because this supplier's products are not listed.
Andrew J. Suh, et al.,
bioRxiv - Cell Biology 2023
Quote:
... gp120 of HIV-2 Group M Subunit B (Catalog number 40404-V08H, Sino Biological), S1 of SARS CoV-2 spike protein (Catalog number 40591-V08H ...
-
No products found
because this supplier's products are not listed.
Xiaoling Cao, et al.,
bioRxiv - Pathology 2020
Quote:
... anti-CD68 and anti-human immunodeficiency viruses (HIV)-1 p24 antibodies were obtained from Novus Biologicals (Littleton, CO, USA). The anti-His tag antibodies were obtained from Proteintech (Rosemont ...
-
No products found
because this supplier's products are not listed.
Ashley M Ngo, et al.,
bioRxiv - Microbiology 2019
Quote:
... Dengue virus NS4B protein (Genetex, GTX124250) at 1:1000 ...
-
No products found
because this supplier's products are not listed.
Joana L. Fernandes, et al.,
bioRxiv - Physiology 2023
Quote:
... protein kinase B (Akt) (1:1000, Cell Signaling Technologies, Madrid, Spain), insulin degrading enzyme (IDE ...
-
No products found
because this supplier's products are not listed.
Lijun Cong, et al.,
bioRxiv - Microbiology 2021
Quote:
... prior to cell supernatants being collected for quantification of virus production by HIV-1 p24 ELISA (XpressBio).
-
No products found
because this supplier's products are not listed.
Muzamil Majid Khan, et al.,
bioRxiv - Physiology 2019
Quote:
... The hPCLS were treated with 10ng/ml recombinant human TGFß 1 protein (R&DSystems#240-B-010) and 25µM (in 750µl of DMEM) of GM6001 (Merck#CC1010). Lived dead staining was carried out using calcein-am/ ethidium homodimer kit (ThermoFisher#L3224 ...
-
No products found
because this supplier's products are not listed.
Alexander E. P. Loftus, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 10 ng/mL human b-FGF (Peprotech; #100-18b), and 1 µg/mL laminin-I (R&D Systems ...
-
No products found
because this supplier's products are not listed.
Sanne van der Niet, et al.,
bioRxiv - Cell Biology 2020
Quote:
Severe combined immunodeficiency (SCID) mice were purchased from Charles River and were aerosol infected with M ...
-
No products found
because this supplier's products are not listed.
Nicholas J. Rettko, et al.,
bioRxiv - Immunology 2023
Quote:
... anti-human HLA-B (Proteintech, 17260), anti-human Glut1 (Abcam ...
-
No products found
because this supplier's products are not listed.
Olivia S. Shin, et al.,
bioRxiv - Immunology 2024
Quote:
B cells were eluted from PBMCs using a MACS Human B Cell isolation kit (Miltenyi Biotec). B cells were stained with rGP38 (IbAr10200 ...
-
Recombinant Antigen
Cat# REC31713-100,
100µg USD $508.0
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Subhasis Mahari, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Human Immunodeficiency Virus (HIV) and Avian Influenza Virus (AIV) Ag were obtained from The Native Antigen Company (Oxford, UK). Aurdino software has been used in the in-house built device and the hardware include a printed Circuit Board (PCB) ...
-
No products found
because this supplier's products are not listed.
Xiaonan Zhang, et al.,
bioRxiv - Microbiology 2020
Quote:
... The blood samples from enrolled patients were tested for hepatitis B virus surface antigen (HBsAg) and hepatitis B virus e antigen (HBeAg) by Abbott AXSYM HBsAg (normal ...
-
No products found
because this supplier's products are not listed.
Kevin O. Saunders, et al.,
bioRxiv - Immunology 2020
Quote:
... ZIKV envelope or HIV-1 proteins were coated overnight at 4°C on the wells of 384-well plates (Corning Life Sciences). Each protein was coated at 2 μg/mL in 15 μl in sodium bicarbonate ...
-
No products found
because this supplier's products are not listed.
Madhubanti Basu, et al.,
bioRxiv - Immunology 2021
Quote:
... were coated overnight at 4°C with 50 μl of 5 μg/ml HIV-1 Env gp140 SF162.B (NIH AIDS Reagent Program) or 1 μg/ml anti-human IgG or IgM (Jackson Immunoresearch, West Grove, PA) in PBS ...
-
No products found
because this supplier's products are not listed.
Taeyong Kwon, et al.,
bioRxiv - Microbiology 2023
Quote:
The whole genome sequence of the mink-derived H5N1 clade 2.3.4.4b virus was determined using the Illumina MiSeq sequencing platform (Illumina, San Diego, CA, USA). Briefly ...
-
No products found
because this supplier's products are not listed.
Alexandra Maslennikova, et al.,
bioRxiv - Microbiology 2021
Quote:
The levels of HIV-1 core protein Gag in cell supernatants were quantified using HIV-1 p24 ELISA Kit (Zeptometrix, Bufalo NY, USA) in accordance to the manufacturer’s instruction ...
-
No products found
because this supplier's products are not listed.
Rachael C. Kretsch, et al.,
bioRxiv - Biophysics 2023
Quote:
... The DMS modified RNA was reverse transcribed by MarathonRT Reverse Transcriptase (Kerafast #EYU007), and the 2A3 modified RNA was reverse transcribed by SuperScript II Reverse Transcriptase (Thermo Fisher #18064022 ...
-
No products found
because this supplier's products are not listed.
Tsukasa Oikawa, et al.,
bioRxiv - Cell Biology 2023
Quote:
... HMLE cells were generated by introducing SV40 large T-antigen and human telomerase reverse transcriptase into a primary culture of normal mammary epithelial cells (Lonza). HMLE cells were cultured in MEGM ...
-
No products found
because this supplier's products are not listed.
J.K. Bruce, et al.,
bioRxiv - Cell Biology 2024
Quote:
... 800ng-1μg of RNA was reverse transcribed in a mix containing M-MLV reverse transcriptase (Invivogen), dNTP mix (Biobasic) ...
-
No products found
because this supplier's products are not listed.
Catherine M. Porter, et al.,
bioRxiv - Bioengineering 2023
Quote:
Primary antibodies and dilutions included biotin anti-human LTL (1:300, B-1325, Vector Laboratories, RRID:AB_2336558), goat anti-human GATA3 (1:20 ...
-
Chromatographically purified dimeric form with M.W. of 66 kDa and 51 kDa. A solution in 10 mM...
Cat# LS05003,
200 un, $95.00
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Sumit Handa, et al.,
bioRxiv - Biochemistry 2020
Quote:
Reverse transcription reactions with Moloney Murine Leukemia Virus (MMLV) RT (BioBharati Life Sciences Pvt. Ltd) and HIV RT (Worthington Biochemical) were carried out according to the manufacturer’s directions using primer P5 (Table S1) ...
-
No products found
Lucie Lanikova, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human HC cells immortalized by human telomerase reverse transcriptase (hTERT) expression were purchased from Applied Biological Materials (cat. no. T0570). The cell lines used were not further authenticated ...
-
No products found
because this supplier's products are not listed.
Aparajitha Vaidyanathan, et al.,
bioRxiv - Microbiology 2022
Quote:
HIV virus was produced by transient transfection of HEK293T cells using Polyethyleneimine (PEI, 1 mg/ml, linear, MW 25000, Polysciences Inc.). Pseudotyping of GFP-HIV (HIV-1 NL4-3 ΔEnv EGFP Reporter Vector ...
-
No products found
because this supplier's products are not listed.
Harold Marcotte, et al.,
bioRxiv - Immunology 2021
Quote:
... The SARS-CoV-2 S2 N defined peptide pool contains 41 synthetic peptides binding to human HLA derived from the S and N proteins of the SARS-CoV-2 virus (#3620-1, Mabtech AB)58.
-
No products found
because this supplier's products are not listed.
William F. Richter, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 1 μg RNA was used for reverse transcription with 0.5 μl MMLV HP reverse transcriptase (Lucigen cat # RT80125K) per 20 μl rxn ...
-
No products found
because this supplier's products are not listed.
Christoph Kreer, et al.,
bioRxiv - Immunology 2022
Quote:
Serum samples from HIV-1-infected individuals were heat-inactivated at 56 °C for 40 min and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Laura E. Doepker, et al.,
bioRxiv - Immunology 2020
Quote:
... The double-labeled cells were coated with clade A gp120 (BL035.W6M.Env.C1, (Wu et al., 2006)) or clade C gp41 ectodomain (C.ZA.1197MB, Immune Technology Corp, New York, NY) (Rousseau et al. ...
-
No products found
because this supplier's products are not listed.
Ann-Kathrin Mattes, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Media containing virus particles was mixed with PEG-it virus precipitation solution (System Biosciences, #LV810A-1) and incubated at 4°C overnight ...