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No products found
because this supplier's products are not listed.
Marlou L. Dirks, et al.,
bioRxiv - Physiology 2023
Quote:
... Arterialized serum samples were used to determine insulin concentrations (Human insulin ELISA kit, DX-EIA-2935; Oxford Biosystems Ltd, Milton Park, UK). Serum NEFA concentrations were measured spectrophotometrically in arterialized venous and deep-venous serum samples (FA115 kit ...
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No products found
because this supplier's products are not listed.
Marion Thauvin, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and luciferase activity was measured every 1 h over 4 h with a 96-well plate luminometer (Tristar, Berthold) as described in the HiBit assay kit (Promega).
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No products found
because this supplier's products are not listed.
Aleksandr Ianevski, et al.,
bioRxiv - Microbiology 2020
Quote:
We measured the IgG and IgM in human serum using Epitope Diagnostics enzyme linked immunosorbent assays (ELISA) according to manufacturer specifications (Epitope Diagnostics, San Diego, CA). Background-corrected OD values were divided by the cutoff to generate signal-to-cutoff (s/co ...
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Glory Nasseri, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Human PRG-1 obtained from DNASU Plasmid Repository (The Biodesign Institute/Arizona State University ...
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No products found
because this supplier's products are not listed.
Yunxia Tang, et al.,
bioRxiv - Immunology 2019
Quote:
Human IFN-γ ELISPOT assays were performed by ELISPOT kit (Mab Tech). The plates were washed three times with PBS ...
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No products found
because this supplier's products are not listed.
Jeroen M. Bugter, et al.,
bioRxiv - Cell Biology 2024
Quote:
... followed by 1 h incubation with 25 µL Protein A-agarose beads (RepliGen). Beads were washed five times with 0,1 x PBS after which beads were eluted in SDS sample buffer and incubated for 30 min at 37°C.
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No products found
because this supplier's products are not listed.
Jesse Garcia Castillo, et al.,
bioRxiv - Immunology 2024
Quote:
... Quantification of IgG for samples was done by diluting serum samples and using the Mouse IgG ELISA commercial kit (Molecular Innovations). For treatment ...
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No products found
because this supplier's products are not listed.
Jinqiang Zhang, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 1:200) for 24 h and the secondary antibodies (anti-rabbit IgG-conjugated Alexa Fluorochrome or anti-mouse IgG conjugated Alexa Fluorochrome ...
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No products found
because this supplier's products are not listed.
Jonah C. Rosch, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Fluorescently labeled human serum albumin (HS1-S5-1) was purchased from NANOCS (New York, NY). The starting DNA library ...
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No products found
because this supplier's products are not listed.
Sandor Spisak, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... Cell culture media was changed the following day and lentiviral supernatant was harvested 24 h and 48 h later and filtered through a 0.22 μm filter (Celltreat). Lentiviruses were aliquoted and stored at – 80 °C until use.
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No products found
because this supplier's products are not listed.
Shogo Yoshimoto, et al.,
bioRxiv - Microbiology 2023
Quote:
... Peak fractions containing the protein complex were dialyzed using a 12-14 kD MWCO dialysis membrane (Spectrum Laboratories, Rancho Dominguez, CA) against Elugent buffer B (25 mM Tris-HCl ...
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No products found
because this supplier's products are not listed.
Heejoo Kim, et al.,
bioRxiv - Immunology 2020
Quote:
... H-Rink-Amide-ChemMatrix (RAM) was purchased from Biotage. N,N-diisopropylethylamine (DIEA) ...
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No products found
because this supplier's products are not listed.
Huu Tuan Nguyen, et al.,
bioRxiv - Bioengineering 2023
Quote:
Human umbilical vein endothelial cells (ECs, Angio-Proteomie, MA, USA) were cultured in Vasculife (LifeLine Cell Technology ...
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No products found
because this supplier's products are not listed.
Stephanie E. Vargas Abonce, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Cell-permeable recombinant human c-MYC was purchased from Abcam (ab169901) and human GBX2 (hGBX2) and LHX9 (hLHX9) were purchased from Proteogenix. Endotoxins were removed by phase separation according to Aida and Pabst ...
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No products found
because this supplier's products are not listed.
Jitendra S. Kanshana, et al.,
bioRxiv - Genetics 2021
Quote:
... 14:10 h light:dark cycle) with ad libitum access to a control low-fat diet (LFD: Research Diets D12450Hi ...
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No products found
because this supplier's products are not listed.
Pehuén Pereyra Gerber, et al.,
bioRxiv - Microbiology 2021
Quote:
... incubated for 30 min with a PE-labeled anti–human IgG-Fc antibody (Leinco/Biotrend), washed again ...
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No products found
because this supplier's products are not listed.
Elahe Ganji, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Tissues were sectioned at 6μm in the sagittal plane and stained with Hematoxylin and Eosin (H&E) or Toluidine Blue and cover-slipped with an acrylic mounting media (Acrymount, Statlab, McKinnery, TX, USA) or used for immunohistochemistry ...
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No products found
because this supplier's products are not listed.
Taiyi Kuo, Domenico Accili,
bioRxiv - Physiology 2020
Quote:
... and NEFA kit (Wako Diagnostics).
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No products found
because this supplier's products are not listed.
Donald Iain MacDonald, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Tacr1 was subcloned along with a synthesized human G-protein α-subunit gene Gα15 and GCaMP6s it into the lentiviral plasmid backbone pLV-CMV-PGK-Hyg (Cellomics Technology, LVR-1046) to create the final lentiviral plasmid pLV-CMV-GCaMP6s-P2A-TACR1-T2A-hG15-PGK-Hyg ...
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No products found
because this supplier's products are not listed.
Eun Jeong Lee, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Plasma ACTH concentrations were measured using the ACTH ELISA kit (MD Biosciences), according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Min-Young Noh, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and CSF NfLs were measured with an ELISA kit (UmanDiagnostics AB, Umeå, Sweden). HC samples were collected from ALS patient spouses after obtaining consent.
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No products found
because this supplier's products are not listed.
Per Nilsson, et al.,
bioRxiv - Neuroscience 2020
Quote:
... After a 24-h treatment with 1 μM SRIF (Peptide Institute #4023), 1 μM cyclo-SST (Tocris #3493) ...
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No products found
because this supplier's products are not listed.
Madelon M.E. de Jong, et al.,
bioRxiv - Immunology 2023
Quote:
... slides were incubated with anti-human CD15 (1:10, C3D-1, Diagnostic BioSystems) for 1 hour at 37°C ...
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No products found
because this supplier's products are not listed.
Hye Sung Kim, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and hydrocodone (HDC, Cerilliant, H-003) at a concentration of 4 nM ...
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No products found
because this supplier's products are not listed.
Min Pan, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Cas9 activity was tested using the CRISPRtest™ Functional Cas9 Activity Kit for Human Cells (Cellecta, #CRTEST). The clone with normal viability and high Cas9 activity (> 95% ...
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No products found
because this supplier's products are not listed.
Lindsey S. Marmont, et al.,
bioRxiv - Microbiology 2020
Quote:
... bovine serum albumin (BSA) in TBS-T for 1 h at room temperature and probed with 10 μg/mL of WFL-HRP (EY Laboratories) in 2% (w/v ...
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No products found
because this supplier's products are not listed.
Dylan J. Drakes, et al.,
bioRxiv - Immunology 2022
Quote:
... Human Panc-1 (HLA A*11:01+, mKRAS-G12D+) GFP/LUC cells (GenTarget Inc., catalog no. SC068-LG) were maintained in DMEM as described above ...
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No products found
because this supplier's products are not listed.
Dora Pinto, et al.,
bioRxiv - Immunology 2020
Quote:
... isolate BetaCoV/Wuhan-Hu-1/2019 (accession number MN908947) was codon optimized for human cell expression and cloned into the phCMV1 expression vector (Genlantis). Expi-CHO cells were transiently transfected with phCMV1-SARS-CoV-2-S ...
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No products found
because this supplier's products are not listed.
Kaushik Inamdar, et al.,
bioRxiv - Biophysics 2021
Quote:
... rabbit polyclonal anti human ALIX (Covalab – pab 0204).
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No products found
because this supplier's products are not listed.
Yuichi Mitsui, et al.,
bioRxiv - Immunology 2022
Quote:
Human PBMCs were purchased from Precision for Medicine, Inc ...
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No products found
because this supplier's products are not listed.
Dennis E.M. de Bakker, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Human tissue was used for immunofluorescent staining as described (Kruithof 2020, CVR) using the following antibodies: anti-cTnI (Hytest, #4T21, 1:1000), anti-Prrx1 (Gift from Tenaka lab ...
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No products found
because this supplier's products are not listed.
Takahiro Sanada, et al.,
bioRxiv - Microbiology 2022
Quote:
... AB human serum (Pel-Freez Biologicals, Rogers, AR, USA) was used as control serum.
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No products found
because this supplier's products are not listed.
Amy L. Kimble, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Frozen mouse and human brain tissues were mechanically homogenized (Next Advance Bullet Blender BB724M with 3.2 mm stainless steel beads using setting 4 for 4min at 4C) ...
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No products found
because this supplier's products are not listed.
James M. Pemberton, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and allowed to grow for 24 h before staining with the nuclear dye DRAQ5 (5nM; Biostatus). Cells were imaged (>15 field of views ...
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No products found
because this supplier's products are not listed.
Dillon C. Malloy, Marie-Pascale Côté,
bioRxiv - Neuroscience 2023
Quote:
... H-reflexes were evoked using an isolated pulse stimulator (Model 2100, A-M Systems, Sequim, WA, USA) delivering single ...
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No products found
because this supplier's products are not listed.
Maria Czarnek, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... After 48 h the transduced cells were selected for 7 days with puromycin (Bioshop Canada Inc, Burlington, Canada) at a concentration of 1 μg/ml for human cell lines ...
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No products found
because this supplier's products are not listed.
Jayan Rammohan, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... The cells were grown overnight for 16 h at 37 °C in an incubator shaking at 300 rpm (Benchmark Scientific). Then ...
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No products found
because this supplier's products are not listed.
Laasya Samhita, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... and captured bright field and fluorescence images at intervals of 2 min for 10 h at 40X magnification using an EMCCD camera (Photometrics Prime). We imaged ∼160 channels at constant flow and temperature and carried out preliminary image editing using ImageJ and used a custom MATLAB code (MathWorks ...
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No products found
because this supplier's products are not listed.
MU Wagenhäuser, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... two-color analysis of murine or human platelet activation was performed using fluorophore-labeled antibodies for P-selectin expression (Emfret Analytics, Wug.E9-FITC ...
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No products found
because this supplier's products are not listed.
K Pilarzyk, et al.,
bioRxiv - Neuroscience 2022
Quote:
... PDE11A (FabGennix PD11-112 at 1:100; Aves custom PDE11A4 #1 at 1:10,000; Fabgennix PPD11A-140AP at 1:1000; Fabgennix PPD11A-150AP at 1:500), NF-L (Cell Signaling #2837 at 1:10,000) ...
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No products found
because this supplier's products are not listed.
Wadie D. Mahauad-Fernandez, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... A 1:1 Luminol and peroxide (Protein Simple) mixture was then added to generate chemiluminescent light ...
-
No products found
because this supplier's products are not listed.
Laurent Jacob, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Rabbit anti–mouse LYVE-1 (11-034, AngioBio, 1:800), Goat anti– human PROX1 (AF2727 ...
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No products found
because this supplier's products are not listed.
Wensheng Chen, Darrell Pilling, Richard H. Gomer,
bioRxiv - Immunology 2022
Quote:
... or 1 or 10 μM IN-1 (AdooQ, Irvine, CA) from a 10 mM stock in DMSO (VWR ...
-
No products found
because this supplier's products are not listed.
Hua He, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... or NKX2-1 antibody (Seven Hills Bioreagents, WRAB-1231, 1:1000) followed by incubation with TrueBlot HRP-Conjugated secondary antibody (Rockland Immunochemicals Inc ...
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No products found
because this supplier's products are not listed.
Ankita B. Jaykumar, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and mycoplasma-free (e-Myco Kit, Boca Scientific or Universal Mycoplasma Detection Kit 30-1012K ...
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No products found
because this supplier's products are not listed.
Akash D. Chakraborty, et al.,
bioRxiv - Physiology 2023
Quote:
... SERCA2 (1:5000, Badrilla), CSQ2 (1:3000 ...
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No products found
because this supplier's products are not listed.
Michael S. Breen, et al.,
bioRxiv - Neuroscience 2019
Quote:
... The e-Myco Mycoplasma PCR Detection Kit (Bulldog Bio, 25233) and the MycoAlert Mycoplasma Detection Kit (Lonza ...
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No products found
because this supplier's products are not listed.
Maria Florencia Ercoli, et al.,
bioRxiv - Plant Biology 2022
Quote:
... 1% sucrose (pH 5.8) in 0.3% Gellex (Gellan Gum CAS#71010-52-1 Caisson Laboratories) supplemented with or without 1µM of the selected kinase inhibitor (see Supplemental Table S5 for a description of the compounds tested in this study) ...
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No products found
because this supplier's products are not listed.
Eric A. Kirk, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Anesthesia was induced with isoflurane (1-5%, Kent Scientific), eye lubricant was applied ...
-
No products found
because this supplier's products are not listed.
James T. McKenna, et al.,
bioRxiv - Neuroscience 2020
Quote:
Mice were deeply anesthetized with isoflurane (1-3%) and viral injections were performed using a 1 µl Hamilton syringe (Cat#7001KH, Point Style 3, Hamilton Company, Reno, NV, USA), targeting BF (AP +0.14 mm ...