-
No products found
because this supplier's products are not listed.
Kazuhide Yahata, et al.,
bioRxiv - Microbiology 2021
Quote:
... were cultured at 37°C with 5% CO2 on fibronectin-coated 6-channel μ-Slides (ibidi, Germany) and grown to confluence in DME/F12 medium (Thermo Fisher ...
-
No products found
because this supplier's products are not listed.
Florian J. Bock, et al.,
bioRxiv - Cell Biology 2020
Quote:
... S63845 (Chemgood, C-1370), Sytox Green (Thermo ...
-
No products found
because this supplier's products are not listed.
Harriet E. Allan, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Aggregation was stimulated with TRAP-6 (25μM) in a 96-well plate (15 minutes, 750rpm, 37°C; BioShake IQ, Quantifoil Instruments) and fixed with 1% formalin (Sigma) ...
-
No products found
because this supplier's products are not listed.
Dominic Esposito, et al.,
bioRxiv - Biochemistry 2020
Quote:
Harvested culture supernatants were clarified by centrifugation (4000 x g, 20 min, 4°C) followed by filtration (catalog# 12993, Pall Corporation, Port Washington, NY). When used in column chromatography ...
-
No products found
because this supplier's products are not listed.
Hannah L. Bernstein, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and perfused at 6-7 mL/min by a peristaltic pump (#Masterflex C/L; Cole-Parmer) on an infrared differential interference microscope (#BX51WI ...
-
No products found
because this supplier's products are not listed.
V D’Arienzo, et al.,
bioRxiv - Microbiology 2020
Quote:
... cDNA synthesis was performed with 0.25-1 µg of RNA in a 20 µL total reaction volume using a random hexamer/oligo dT strand synthesis kit as per the manufacturer’s instructions (10 min at 25°C; 15 min at 42°C; 15 min at 48°C; SensiFast, Bioline). All oligonucleotide sequences are listed in Table 1.
-
No products found
because this supplier's products are not listed.
Gyu Rie Lee, et al.,
bioRxiv - Biochemistry 2023
Quote:
... anti-c-Myc fluorescein isothiocyanate (anti-c-Myc-FITC, Immunology Consultants Laboratory) and streptavidin-phycoerythrin (SAPE ...
-
No products found
because this supplier's products are not listed.
Matthew E Sherman, et al.,
bioRxiv - Microbiology 2023
Quote:
... Bacteria were grown at 30°C or 37°C in Lysogenic Broth (Teknova) and Tryptic Soy broth (TSB ...
-
No products found
because this supplier's products are not listed.
Jelena Kolic, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Samples were stored at −20 °C and insulin secretion was quantified using human insulin radioimmunoassay kits (Cedarlane).
-
No products found
because this supplier's products are not listed.
Anna Pepe, et al.,
bioRxiv - Cell Biology 2021
Quote:
Human neuroblastoma (SH-SY5Y) cells were cultured at 37 °C in 5% CO2 in RPMI-1640 (Euroclone), plus 10% fetal bovine serum and 1% penicillin/streptomycin ...
-
No products found
because this supplier's products are not listed.
Tomoyuki Hatano, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The plates were incubated for two days at 30 □C before the X-Gal (#RC-212, G-Biosciences, USA) overlay ...
-
No products found
because this supplier's products are not listed.
Seong Beom Ahn, et al.,
bioRxiv - Biochemistry 2020
Quote:
... the peptide trap was switched in-line with a cHiPLC C18 column (15 cm x 200μm, 3μm, ChromXP C18-CL, 120 Å, 25°C, SCIEX) and peptides were eluted using a linear 120min gradient from 5 % acetonitrile to 35 % mobile phase B (B ...
-
No products found
because this supplier's products are not listed.
Chloe R. Koulouris, et al.,
bioRxiv - Biophysics 2021
Quote:
Human malonate-bound SR was crystallised at 20 °C in 48-well MRC Maxi sittingdrop plates (Molecular Dimensions) in 25% PEG 3350 ...
-
No products found
because this supplier's products are not listed.
Minghao Chen, et al.,
bioRxiv - Biochemistry 2023
Quote:
... lysates were clarified by centrifugation (17,000 x rpm for 40 min at 4 °C) and incubated with Strep-Tactin Sepharose (IBA Lifesciences) at 4 °C overnight ...
-
No products found
because this supplier's products are not listed.
Jing Yang (John) Wang, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 100 mM glycine to glow-discharged C-flat CF-2/2 C-T-grids (TED PELLA).
-
No products found
because this supplier's products are not listed.
Min Yao, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and human IgG ELISA kit (Mabtech, #3850-1H-6).
-
No products found
because this supplier's products are not listed.
Gurur Garip, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... anti-c-Caspase3 (Bioss BS-0081R), anti-Beclin1 (S-1353R ...
-
8 Well Chambered Cover Glass with #1 cover glass (0.13-0.16mm), with lid, sterilized. Designed...
Cat# C8-1-N,
48/case, $219.00
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Sandrine B. Lavenus, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 2 mL was cured overnight at 37 °C in each well of a 6-well glass bottom plate (Cellvis, Mountain View, CA). Using a biopsy punch (cat no ...
-
No products found
because this supplier's products are not listed.
Antara Das, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Electrophysiological recordings were performed at room temperature (25 °C) with glass pipettes with open tip resistance of 3-6 MΩ (TW150F-3, World Precision Instruments, & 100µL calibrated pipettes #534332-921,VWR International) ...
-
No products found
because this supplier's products are not listed.
N.I. Márquez, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Body temperature was kept between 36 °C and 37 °C throughout the experiment by using a thermo-regulated blanket (FHC, Inc.). To prevent asphyxia due to accumulation of bronchial secretions ...
-
No products found
because this supplier's products are not listed.
Jun Dai, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 1 ml of 37 °C Recovery Medium (Lucigen, 80026-1) was added and bacteria were grown in round-bottom tubes for 1 h at 37 °C while shaking at 180 r.p.m ...
-
No products found
because this supplier's products are not listed.
Luana dos Santos Ortolan, et al.,
bioRxiv - Pathology 2019
Quote:
Soluble endothelial protein C receptor (sEPCR) was measured with an ELISA kit (Elabscience®, E-EL-M1073) according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Ryan J. Garrigues, et al.,
bioRxiv - Microbiology 2021
Quote:
Purified BBK32-C and a purified autolytic fragment of human C1r (Complement Technologies) were mixed at 5% molar excess BBK32-C and concentrated to 3.5 mg ml-1 in 10 mM HEPES (pH 7.3 ...
-
No products found
because this supplier's products are not listed.
Corinne Benakis, et al.,
bioRxiv - Immunology 2021
Quote:
... slides were incubated in blocking at RT for 1 h before overnight incubation at 4°C with the primary rabbit anti-P2Y12 receptor antibody (1:200, AnaSpec #AS-55043A) and labelling for 1 hour with the secondary antibody AF488 goat anti-rabbit ...
-
No products found
because this supplier's products are not listed.
Selma Belhimeur, et al.,
bioRxiv - Immunology 2022
Quote:
... 95°C for 10 min, 40 cycles of 15 s at 95°C, and 60°C for 1 min; ABI PRISM 7000 Sequence Detection System ...
-
No products found
because this supplier's products are not listed.
Huimin Liu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... and 0.5% Triton X-100 were used to permeabilized at 4°C with (Solarbio Life Science, Beijing, China). Following a wash with PBS ...
-
No products found
because this supplier's products are not listed.
Wren E. Michaels, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Quantitative analysis of the CFTR B and C-bands was performed using Compass software (Protein Simple).
-
No products found
because this supplier's products are not listed.
Zachory M. Park, et al.,
bioRxiv - Genetics 2023
Quote:
... Cells were lysed using a FastPrep (MP) bead beater (5 x 90 second runs at 4°C) and acid-washed glass beads (BioSpec), centrifuged ...
-
No products found
because this supplier's products are not listed.
Nasim Saadat, et al.,
bioRxiv - Genetics 2024
Quote:
Initial small crystals were formed at 4 °C in 3.5 M sodium formate (Condition X of Index Screen; Hampton Research) in a sitting drop format as part of a robot-controlled screen in 96 well plates (200 nL drop size ...
-
No products found
because this supplier's products are not listed.
Theresa K Leslie, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... then were incubated for 10 minutes at 21 °C in 1 μM 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM, Biotium) in PSS ...
-
STING inhibitor C-176 is a potent, small-molecule inhibitor of STING, a central signaling...
Cat# S6575, SKU# S6575-25mg,
25mg, $270.00
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Georgia Balsevich, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and Compound C (Selleck Chemicals, S7306), were used in all in vitro and in vivo experiments ...
-
No products found
because this supplier's products are not listed.
Matthew L. Baum, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Slides were blocked for 1hr in blocking solution (antibody buffer with 5% BSA and 0.8% Triton X-100) followed by incubation overnight at 4°C with 1:1000 rabbit anti-Csmd1 antibody (Lund, produced by Agrisera, Sweden) in 4:1 antibody buffer (150 mM NaCl ...
-
No products found
because this supplier's products are not listed.
B. Weiss, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 500 μg of protein was incubated overnight at 4°C with m7GTP agarose beads (Jena biosciences, #AC-155S). Beads were washed with cap binding buffer and suspended in 20 μL Laemmli buffer ...
-
No products found
because this supplier's products are not listed.
Thibault Rosazza, et al.,
bioRxiv - Immunology 2020
Quote:
... and cultured for 6 days at 37°C in a 7.5% CO2 air atmosphere in complete DMEM medium (Pan Biotech – P04-03500) containing 4.5 g/L Glucose ...
-
No products found
because this supplier's products are not listed.
David Cluet, et al.,
bioRxiv - Biochemistry 2019
Quote:
... HA tagged proteins were labeled overnight at 4°C with the mouse HA.11 Clone 16B12 Monoclonal Antibody (Eurogentec) 1/2000 in PBS + tween 0.2% (v/v ...
-
No products found
because this supplier's products are not listed.
Sharon Tran, et al.,
bioRxiv - Cell Biology 2023
Quote:
... by their overnight incubation at 56°C followed by 1 hour at 85°C in DirectPCR (Tail) Lysis Reagent (Viagen) supplemented with 0.2 % (v/v ...
-
No products found
because this supplier's products are not listed.
Krzysztof Kucharz, et al.,
bioRxiv - Neuroscience 2020
Quote:
... at 70°C using a mini-extruder (Avanti Polar Lipids) to form nanoparticles.
-
No products found
because this supplier's products are not listed.
Ahmed E.I. Hamouda, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 37°C and a Cascade 512B EMCCD camera (Photometrics, USA) operated with IPlab Spectrum software (Scanalytix ...
-
No products found
because this supplier's products are not listed.
Olubu A. Adiji, et al.,
bioRxiv - Biochemistry 2023
Quote:
... maintained at 27°C in ESF 921 medium (Expression Systems). For transfection ...
-
No products found
because this supplier's products are not listed.
Jian Huang, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... the GLUT3 protein was purified in 0.06% (w/v) 6-cyclohexyl-1-hexyl-ß-D-maltoside (Cymal-6, Anatrace) and concentrated to ~ 40 mg/ml ...
-
No products found
because this supplier's products are not listed.
M. Winant, et al.,
bioRxiv - Genetics 2022
Quote:
Drosophila stocks were reared at 25°C on Nutrifly (Genesee Scientific) for the initial DGRP validation screen ...
-
No products found
because this supplier's products are not listed.
Rosa Machado, et al.,
bioRxiv - Biophysics 2019
Quote:
... we warmed the objective to 37 °C using an objective heater (Bioptechs). In some experiments we used a custom-made microscope incubation chamber ...
-
No products found
because this supplier's products are not listed.
Emily Doucette, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Layers of adhesive cement (C&B Metabond) followed by dental cement (Stoelting) were spread over the surgical site to secure the optical fiber implant to the skull ...
-
No products found
because this supplier's products are not listed.
Paul D. Simonson, Itzel Valencia, Sanjay S. Patel,
bioRxiv - Immunology 2022
Quote:
... The standard Akoya CODEX protocols were followed (Akoya CODEX manual, revision C). For tyramide-barcode staining ...
-
No products found
because this supplier's products are not listed.
Deike J. Omnus, et al.,
bioRxiv - Microbiology 2021
Quote:
... Samples were boiled at 65 °C for 10 minutes and separated by SDS-Page (Bio-Rad 4–20 % Mini-PROTEAN® TGX Stain-Free™ protein gel), visualized by InstantBlue protein stain (Expedeon ...
-
No products found
because this supplier's products are not listed.
Qing Tang, et al.,
bioRxiv - Cell Biology 2022
Quote:
BSC-1 or U2OS cells (BS-C-1; CCL-26; American Type Culture Collection) were routinely maintained in MEM or DMEM media (ThermoFisher ...
-
No products found
because this supplier's products are not listed.
Ida Søgaard Larsen, et al.,
bioRxiv - Microbiology 2023
Quote:
... and C-peptide by Mouse C-peptide ELISA kit (Crystal Chem) following the manufacturers’ protocols.
-
No products found
because this supplier's products are not listed.
Simone Franziska Glaser, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Human brain vascular pericytes (passages 2-6; #1200, ScienCell) were cultured in Dulbecco’s Modified Eagle Medium Glutamax (#31966-021 ...
-
No products found
because this supplier's products are not listed.
Camila Marques-da-Silva, et al.,
bioRxiv - Immunology 2023
Quote:
... 6-8×105 primary mouse or human (obtained from BioIVT) hepatocyte cultures were infected with 2-4×104 sporozoites in each well of a 6-well plate ...
-
No products found
because this supplier's products are not listed.
Erin A. Nekritz, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... we co-transfected the cells with 50 ng of pMIR-REPORT constructs containing the 3’UTR sequences together with 50 ng of a Renilla housekeeping control plasmid and 100 nM of miR-424 or miR-503 mimics or a miRNA negative control (Dharmacon #C-300717-05, #C-300841-05, or #CN-001000-01-05, respectively) using the TransIT-X2 reagent (Mirus 6003). 24 hours after transfection ...