-
No products found
because this supplier's products are not listed.
Michelle E. Gray, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and incubated overnight at 4°C with goat anti-human IgG (1:200; Jackson ImmunoResearch Laboratories ...
-
No products found
because this supplier's products are not listed.
David W. Hammers, et al.,
bioRxiv - Cell Biology 2021
Quote:
... plasmid was generated by adding the C-terminal 20 amino acids of human H-Ras (NCBI Accession No. NP_005334) to a tagRFP-C vector (Evrogen) modified with a S158T point mutation to enhance photostability (Shaner et al. ...
-
No products found
because this supplier's products are not listed.
Calvin Yeager, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius, 10 kDa MWCO) to 2 – 5 mL for preparative gel filtration ...
-
No products found
because this supplier's products are not listed.
Sophie Lanciano, et al.,
bioRxiv - Genomics 2023
Quote:
Two micrograms of genomic DNA were sonicated for 6 cycles (6 s on, 90 s off) at 4 °C with a Bioruptor NGS (Diagenode), generating average fragments of 1 kb ...
-
No products found
because this supplier's products are not listed.
Laura Sen-Martín, et al.,
bioRxiv - Physiology 2024
Quote:
... 2 x 106 cells were seeded on p35 culture plates (Mattek, P35G-0-10-C) covered with matrigel solution (Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Jun Tominaga, et al.,
bioRxiv - Plant Biology 2020
Quote:
... a large 6 x 6 cm chamber (6800-13; LI-COR) was used to maximize measurement precision ...
-
No products found
because this supplier's products are not listed.
Swati Gupta, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and Western Blotting samples were denatured at 94°C for 5 minutes in 3 x SDS sample buffer (150mM Tris pH 8, 6% SDS, 0.3M DTT ...
-
No products found
because this supplier's products are not listed.
O Bogen, et al.,
bioRxiv - Neuroscience 2024
Quote:
... psi ε receptor for activated C kinase (ψεRACK) (27) was purchased from Biomatik (Wilmington, DE, USA), and the proinflammatory cytokine prostaglandin-E2 (PGE2 ...
-
No products found
because this supplier's products are not listed.
Ryan P. McNamara, et al.,
bioRxiv - Immunology 2022
Quote:
... and 1:750 for Fcγ-receptor binding) overnight at 4°C in 384 well plates (Greiner Bio-One, Germany). Secondary antibodies were PE-conjugated and incubated with samples at room temperature for 1 hour at a 1:100 dilution in sterile-filtered Assay Buffer (1X PBS ...
-
No products found
because this supplier's products are not listed.
Yakun Wu, et al.,
bioRxiv - Immunology 2021
Quote:
... the samples were diluted 10 x with the Immunoassay Buffer C (Cayman chemical, # 401703). The cGAMP amount was determined by ELISA assays according to the manufacture’s protocols (Cayman chemical ...
-
No products found
because this supplier's products are not listed.
Robert Düster, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and the thermal stability was monitored from 20°C to 90°C at a heating rate of 1.5°C/min using the PR.ThermControl software (NanoTemper).
-
No products found
because this supplier's products are not listed.
Florian Wiede, et al.,
bioRxiv - Immunology 2019
Quote:
... Sections were then incubated overnight in (4°C) with anti-c-ErbB2/c-Neu (Ab-3) mouse mAb (3B5) (1:500, Calbiochem). After washing with PBS ...
-
No products found
because this supplier's products are not listed.
S. Gulberk Ozcebe, et al.,
bioRxiv - Bioengineering 2020
Quote:
... cytochrome c (Novus Biologicals, 7H8.2C12), cleaved caspase-3 (R&D System ...
-
No products found
because this supplier's products are not listed.
Olena Kim, et al.,
bioRxiv - Neuroscience 2023
Quote:
... samples were washed 3–4 times 10 min each with fresh acetone chilled to −90°C and left at the same temperature for 6 hours in acetone containing 2% osmium (Science services (EMS)) and 0.2% uranyl acetate (Serva) ...
-
No products found
because this supplier's products are not listed.
Shiyou Zhu, et al.,
bioRxiv - Microbiology 2021
Quote:
Potential receptor proteins with C-terminal Flag tag were transiently expressed in HEK293F cells using polyethylenimine (PEI, Polysciences). 36 h following transfection ...
-
LC Laboratories' Product Number D-3197 - Dorsomorphin, Free Base (AMPK Inhibitor I, BML-275, BMP...
Cat# D-3197, SKU# D-3197_10mg,
10 mg, $57.00
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Jacqueline M. Bliley, et al.,
bioRxiv - Bioengineering 2020
Quote:
... plus 6 µm CHIR99201 (C-6556, LC laboratories) for 48 hours ...
-
No products found
because this supplier's products are not listed.
Margrethe A. Boyd, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... Protein expression was monitored at 37°C in a SpectraMax i3x plate reader (Molecular Devices). GFP was monitored at ex ...
-
No products found
because this supplier's products are not listed.
Laura Kuschmierz, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... and 76°C (New Brunswick Innova 44 incubator ...
-
No products found
because this supplier's products are not listed.
Selene García-Hernández, et al.,
bioRxiv - Plant Biology 2024
Quote:
... Protein extracts were there incubated at 4 C for 1 h with GFP-Trap beads (Chromotek) and unspecific binding was minimized by several washes with wash buffer (the same as extraction buffer but with only 0.2 % NP-40) ...
-
No products found
because this supplier's products are not listed.
Hayato Hirai, Yuki Shogaki, Masamitsu Sato,
bioRxiv - Cell Biology 2021
Quote:
... Mounted cells were observed at 25°C or 36°C using a microscope (IX71, Olympus) with the DeltaVision-SoftWoRx system (Applied Precision) ...
-
No products found
because this supplier's products are not listed.
Quentin S. Fischer, et al.,
bioRxiv - Neuroscience 2023
Quote:
... then incubated overnight in an avidin-biotin-horseradish peroxidase complex at 4°C with 0.1% Triton X-100 (Vector Elite; Vector Laboratories). The tissue was counterstained with cresyl violet and cover slipped with dibutyl phithalate (Electron Microscopy Sciences) ...
-
No products found
because this supplier's products are not listed.
Gabriel A. Suárez, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... was recorded every 10 minutes following a brief shaking step (15 seconds with a 6 mm amplitude) as these cultures were incubated at 30°C in an Infinite M200 Pro microplate reader (Tecan).
-
No products found
because this supplier's products are not listed.
Shivneet K. Gill, et al.,
bioRxiv - Biochemistry 2024
Quote:
Protein toxins were incubated with either human sera (Human Serum age 4-6, Innovative Research) or PBS in a 1:1 ratio ...
-
No products found
because this supplier's products are not listed.
Isabel Mulet, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... DNA was hydrolysed for 6 h at 37°C using DNA degradase plus (Zymo Research). Mass spectrometry quantification was performed as previously described36 in the Babraham Institute mass spectrometry facility using three individual E18.5 wild-type and three individual E18.5 Tet3 knockout embryos.
-
No products found
because this supplier's products are not listed.
Alessandra Gallinaro, et al.,
bioRxiv - Immunology 2021
Quote:
... were coated with 2.5 µg/ml of mouse anti-human c-Myc 9E10 mAb (produced in house) or goat anti-human IgG (1:2000, Southern Biotech) for the standard ...
-
No products found
because this supplier's products are not listed.
Victoria L. Castro, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... C-terminal HCFC1 (Bethyl labs Cat # 50- 156-0286 purchased through Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Tomoki Togashi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... anti-mouse protein C polyclonal sheep IgG conjugated with horse-radish peroxidase (GeneTex, Irvine, CA) was incubated for 1 h ...
-
No products found
because this supplier's products are not listed.
Kim T Melville, et al.,
bioRxiv - Microbiology 2023
Quote:
... Protein fluorescence was measured at 25 °C on a CLARIOstar multimode plate reader (BMG Labtech), with excitation at 295 ± 10 nm ...
-
Chromatographically purified. A dialyzed, lyophilized pre-activated powder.
Cat# LS001643,
5x1 mg, $166.00
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Laura Alonso-Herranz, et al.,
bioRxiv - Immunology 2020
Quote:
... the aortas were then cut into small pieces (1-2 mm) and incubated for 45 minutes at 37 °C in a type I collagenase (6 mg/ml, Worthington) and elastase (2.5 mg/ml ...
-
No products found
because this supplier's products are not listed.
Gareth R Hardisty, et al.,
bioRxiv - Immunology 2020
Quote:
... NET formation was stimulated with 100nM PMA and detected with SYTOX™ Green nucleic acid stain (Thermo) at 528nm during 6 hr incubation at 37°c in a Synergy HT (BioTek) plate reader (as previously described).[31] NET formation was confirmed by fluorescence microscopy.
-
No products found
because this supplier's products are not listed.
Yun Huang, et al.,
bioRxiv - Biophysics 2023
Quote:
... The temperature was then set to 30 °C and protein expression was induced by adding 0.2 % arabinose (Goldbio). Cells were grown for additional 16 hours ...
-
No products found
because this supplier's products are not listed.
Hazal Haytural, et al.,
bioRxiv - Neuroscience 2020
Quote:
... pH 6) at 110 °C for 30 min inside a pressure cooker (Biocare Medical). Slides were then washed in PBS with 0.05% Tween-20 (PBS-T ...
-
No products found
because this supplier's products are not listed.
Franziska Jäger, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins were purified at 4 °C on Ni-NTA-Sepharose (Macherey-Nagel) packed in a gravity flow column ...
-
No products found
because this supplier's products are not listed.
Pablo Rougerie, Dianne Cox,
bioRxiv - Cell Biology 2020
Quote:
... The gel was then immediately rinsed with distilled water and incubated overnight at 4°C with a 1mg/mL solution of human whole IgG (CellSciences) or human Fab fragment (Rockland) in 50mM HEPES pH 8 ...
-
No products found
because this supplier's products are not listed.
Qingtao Sun, et al.,
bioRxiv - Neuroscience 2023
Quote:
Biotinylated human IL-6 solution (Acrobiosystems, IL-6-H8218 ...
-
No products found
because this supplier's products are not listed.
Stacia M. Nicholson, Francis A.X. Schanne,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... Fc receptors were blocked for 30 min with Protein A (MP Biomedicals, OH) in Stain Buffer (554656 ...
-
No products found
because this supplier's products are not listed.
Sangwoo Kim, et al.,
bioRxiv - Biophysics 2020
Quote:
... for a dorsal view of the tailbud and imaged at 25°C using a 40x water immersion objective (LD C-Apochromat 1.1 W, Carl Zeiss) on an inverted Zeiss Laser Scanning Confocal (LSM 710 ...
-
No products found
because this supplier's products are not listed.
Christiana Bjorkli, et al.,
bioRxiv - Neuroscience 2022
Quote:
... was set to 6 °C for the storage of collected CSF in 300 μl low-retention polypropylene plastic vials (Harvard Apparatus, Cambridge, MA, USA). Fluorinated ethylene propylene (FEP ...
-
No products found
because this supplier's products are not listed.
Mayumi K. Holly, et al.,
bioRxiv - Microbiology 2020
Quote:
... C-reactive protein (MyBioSource). The optical density was read at 450 nm on an EnVision plate reader (PerkinElmer) ...
-
No products found
because this supplier's products are not listed.
Ya-Juan Wang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... or GST-tagged human GABAA receptor β2 subunit protein (GST-β2) (Abnova, catalog #: H00002561-P01) was mixed with 4 μg of recombinant His-tagged human Hsp47 protein (Novus ...
-
Cat# HY-111947,
inquire
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Miriam Pagin, et al.,
bioRxiv - Genetics 2021
Quote:
... c-Fos/activator protein (AP-1) inhibitor (MedChemExpress), were dissolved in DMSO according to manufacturer’s instructions to obtain the 5 mM ...
-
No products found
because this supplier's products are not listed.
Tyler N. Starr, et al.,
bioRxiv - Microbiology 2022
Quote:
... Proteins were biotinylated overnight at 4°C using a BirA biotinprotein ligase reaction kit (Avidity) and re-purified using the same HisTrap HP affinity method described above before being flash frozen.
-
No products found
because this supplier's products are not listed.
Matarr Khan, et al.,
bioRxiv - Immunology 2023
Quote:
... 6 x 106 lung cells were plated in a 6-well plate (Sarstedt, 83.3920) and restimulated with 50 ng/ml Phorbol 12-myristate 13-acetate (PMA ...
-
No products found
because this supplier's products are not listed.
Aliza A. Le, et al.,
bioRxiv - Neuroscience 2024
Quote:
... (C) (+)-Citronellal (∼96% Alfa Aesar), (D ...
-
No products found
because this supplier's products are not listed.
Johanna Hol Fosse, et al.,
bioRxiv - Microbiology 2023
Quote:
... Erythrocyte suspensions were maintained in 6-well cell culture plates on a microplate shaker (IKA-Werke, 150-200 rpm, 15 °C) for up to 10 days ...
-
No products found
because this supplier's products are not listed.
Fei Liang, et al.,
bioRxiv - Genetics 2020
Quote:
... Antibodies: c-ABL (1:1000; A0282, Abclonal), NOTCH1 (1:1000 ...
-
No products found
because this supplier's products are not listed.
Xiaoyu Sun, et al.,
bioRxiv - Biophysics 2020
Quote:
Plasmids encoding C-terminal eGFP-labeled full-length human LIM proteins were purchased from GeneCopoeia in the m98 vector ...
-
No products found
because this supplier's products are not listed.
Jun-yi Zhu, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... raised against human polyubiquitin-B and polyubiquitin-C (1:100) (BML-PW8810, Enzo Life Sciences); Rabbit polyclonal antibody against protein disulfide isomerase (1:100 ...
-
No products found
because this supplier's products are not listed.
A. Teissier, et al.,
bioRxiv - Animal Behavior and Cognition 2019
Quote:
... AMPA receptors with 10μM 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, Hello Bio Inc), and NMDA receptors with 50 μM d-APV (HelloBio) ...
-
No products found
because this supplier's products are not listed.
Breane G. Budaitis, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and 6% fluorescent (X-Rhodamine or HiLyte647) tubulin (Cytoskeleton Inc) in the presence of the nonhydrolyzable GTP analogue GMPCPP (Jena Bioscience ...