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No products found
because this supplier's products are not listed.
Katarzyna Bogucka-Janczi, et al.,
bioRxiv - Cell Biology 2022
Quote:
shRNAs plasmids were purified from the MISSION® shRNA Human Library (Sigma). Non-targeting control shRNA (shCo ...
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No products found
because this supplier's products are not listed.
Sheikh Tahir Majeed, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... shRNA to human raptor (plasmid#1857) and rictor (plasmid#1853) were purchased from Addgene. The preparation of shRNA infected HEK293 cells have been described previously (28).
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No products found
because this supplier's products are not listed.
Anjali Balakrishnan, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... we used NSMase2 (SMPD3) Human shRNA Plasmid Kit (Origene, Locus ID 55512), which included what we termed shSMPD3 variants A-D and shScr ...
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No products found
because this supplier's products are not listed.
Jun Yang, et al.,
bioRxiv - Cell Biology 2023
Quote:
COL11A1 shRNA plasmid was obtained from NIEHS Genomic core (Thermo Scientific GIPZ Lentiviral shRNA). Lentiviral packaging was produced at the NIEHS Viral Vector Core ...
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No products found
because this supplier's products are not listed.
Anupriya Aggarwal, et al.,
bioRxiv - Microbiology 2021
Quote:
... shRNA plasmids (pool of three shRNA plasmids per target) were obtained directly from Santa Cruz.
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No products found
because this supplier's products are not listed.
Clare Rogerson, et al.,
bioRxiv - Cell Biology 2019
Quote:
... shRNA constructs were obtained from QIAGEN (SureSilencing Akt1 shRNA plasmids) or Origene (HuSH Myh9 shRNA constructs).
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No products found
because this supplier's products are not listed.
Shreya Budhiraja, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... shRNA plasmids were procured from Genecopoeia (Rockville, MD, USA), and overexpression plasmids were obtained from AddGene (Watertown ...
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No products found
because this supplier's products are not listed.
Rohtem Aviram, et al.,
bioRxiv - Cell Biology 2024
Quote:
... MISSION® shRNA lentiviral plasmids (Merck) were used for knockdown of LOX (shLOX1 – TRCN0000045991 ...
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No products found
because this supplier's products are not listed.
Adam G Grieve, et al.,
bioRxiv - Biochemistry 2020
Quote:
... in 35mm plates with 0.5 μg of pLKO shRNA or pLVX expression plasmids (Takara), 0.35 μg pCMV-dR8.2 and 0.15 μg pCMV-VSVG ...
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No products found
because this supplier's products are not listed.
Natalia G. Sampaio, et al.,
bioRxiv - Immunology 2021
Quote:
... and 1 μg shRNA plasmid using Fugene 6 (Promega). The next day ...
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No products found
because this supplier's products are not listed.
Danielle L. Tomasello, et al.,
bioRxiv - Neuroscience 2021
Quote:
... DYKDDDDK (Flag)-tagged human FAM57B plasmid was purchased from Genscript. Hemagglutinin (HA)-tagged human CerS plasmids were generated as described76.
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No products found
because this supplier's products are not listed.
Lisa Baker, et al.,
bioRxiv - Neuroscience 2020
Quote:
... except cells were transduced with AAV5 containing a control scrambled shRNA plasmid or a FL2 shRNA plasmid (Vector Biolabs). shRNA sequences were cloned into a D2-U6 vector ...
-
No products found
because this supplier's products are not listed.
Changhe Ji,
bioRxiv - Neuroscience 2022
Quote:
shRNAs targeting human HNRNPR were cloned into a modified version of pSIH-H1 shRNA vector (System Biosciences) containing EGFP according to the manufacturer’s instructions ...
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No products found
Md Imamul Islam, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Scramble (LV-shRNA-scramble, #LVP015-G) and Trx1 shRNA plasmids (LV-shRNA-Trx1, #488660940395) were purchased from Applied Biological Materials Inc. ...
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No products found
because this supplier's products are not listed.
Ratnadeep Mukherjee, et al.,
bioRxiv - Immunology 2020
Quote:
... 5 µg of NOSIP shRNA plasmids or control TRC2 plasmid was mixed with 100 µl Cell Line Nuceleofector Solution V (Lonza) and added to one million cells ...
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No products found
because this supplier's products are not listed.
Xizi Chen, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Lentivirus expressing short-hairpin RNAs was prepared by transfecting PLKO.1 shRNA plasmids and packaging plasmids containing psPAX2 and pMD2.G into 293T cells using PEI (Polysciences). Collected conditional media containing virus particles were used to transduce cells in the growth media supplemented with Polybrene for 24 hours ...
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No products found
because this supplier's products are not listed.
Shiyi Wang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... a DNA fragment containing hU6 and shRNA was amplified from pLKO.1-shRNA using Phusion High-Fidelity DNA Polymerase (NEB) with primers that introduced SpeI restriction sites (Forward Primer ...
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No products found
because this supplier's products are not listed.
Valerio Taverniti, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... 38HK were transiently transfected with lentiviral shRNA plasmids using TransIT-Keratinocytes Transfection Reagent (Mirus) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Duc T. Tran, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Plasmids expressing human PRDX4-FLAG and Human QSOX1-HA were purchased from Sino Biological Inc ...
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No products found
because this supplier's products are not listed.
Ulli Heydasch, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Transfection of plasmids for knockdown purposes (shRNA-vectors) was performed using FuGene®HD (Roche Diagnostics) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Sanghee Yun, et al.,
bioRxiv - Animal Behavior and Cognition 2023
Quote:
... Each annealed oligonucleotide was ligated into the adeno-associated virus (AAV2) plasmid (pAAV-EGFP-shRNA; Stratagene, La Jolla, CA)(Hommel et al. ...
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No products found
because this supplier's products are not listed.
Ning Yang, et al.,
bioRxiv - Microbiology 2021
Quote:
... Human cGAS (hcGAS) and murine cGAS (mcGAS) plasmids were purchased from Invivogen. Flag-cGAS and V5-cGAS were amplified by PCR and were cloned into pcDNA3.2-DEST plasmids ...
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No products found
because this supplier's products are not listed.
Janet F. Staab, et al.,
bioRxiv - Cell Biology 2020
Quote:
Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...
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No products found
because this supplier's products are not listed.
Cornelius M. Funk, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 1,500–4,000 cells containing either a Dox-inducible non-targeting control shRNA or EIF4EBP1-targeting specific shRNAs were seeded in Costar Ultra-low attachment plates (Corning) for 8–14 d in 150 µl growth medium ...
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No products found
because this supplier's products are not listed.
Meriem Belabed, et al.,
bioRxiv - Immunology 2023
Quote:
Human: Anti-human CD141 (clone M80, BioLegend), anti-human CD45RA (clone HI100 ...
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No products found
because this supplier's products are not listed.
Francesca Puca, et al.,
bioRxiv - Cancer Biology 2020
Quote:
The inducible shRNA was obtained from Cellecta by cloning into a pRSIT16-U6Tet-sh-CMV-TetRep-2A-TagRFP-2A-Puro vector the following hairpin sequence:
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No products found
because this supplier's products are not listed.
Dagan Segal, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and shRNA knockdown was verified by western blotting using rabbit anti-Fli1 (AbCam ab15289) (Figure S3).
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No products found
because this supplier's products are not listed.
Gonzalo Quiroga-Artigas, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Newly synthesized shRNAs were then purified using the Direct-zol™ RNA MiniPrep Kit (Zymo Research), eluted in 35μl of nuclease-free H2O and quantified with a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific) ...
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No products found
because this supplier's products are not listed.
Nataly P. Podolnikova, et al.,
bioRxiv - Cell Biology 2022
Quote:
The cDNA encoding the extracellular N-terminal IgV domain of human CD47 (residues 19-141) was amplified from the plasmid hCD47 VersaClone cDNA (RDC1523, R&D systems) and was cloned in the expression vector pGEX-4T-1 (GE Healthcare) ...
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No products found
because this supplier's products are not listed.
Florent Peglion, et al.,
bioRxiv - Cell Biology 2021
Quote:
... human FGF-basic and human EGF (animal free, Peprotech) at 20 ng/mL ...
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No products found
because this supplier's products are not listed.
Zhiming Chen, et al.,
bioRxiv - Cell Biology 2020
Quote:
... anti-human CD44 or anti-human CD59 (BD Pharmingen) or 5μg/ml Biotin-Tfn (Sigma) ...
-
No products found
because this supplier's products are not listed.
Valeriya Malysheva, et al.,
bioRxiv - Genetics 2022
Quote:
... anti-human-CD19 and anti-human-CD14 (Miltenyi Biotec) and transferred through LD columns (Miltenyi Biotec ...
-
No products found
because this supplier's products are not listed.
Julia Prigann, et al.,
bioRxiv - Microbiology 2023
Quote:
... Human NK cell or Human Monocyte CD14+ Isolation kits (STEMCELL Technologies), respectively ...
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No products found
because this supplier's products are not listed.
Joachim M. Surm, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
... shRNAs were synthesized with AmpliScribe T7-Flash Transcription Kit (Epicentre, Charlotte, NC) for 15 h followed by 15 min treatment with 1 µl of DNase I ...
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No products found
because this supplier's products are not listed.
Ali Akbar Karkhaneh Yousefi, et al.,
bioRxiv - Biophysics 2023
Quote:
... using 10% Human Fn (Human Fn, Promocell) in PBS ...
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No products found
because this supplier's products are not listed.
Gemma E. Hartley, et al.,
bioRxiv - Immunology 2023
Quote:
... Serially diluted recombinant human IgG1 or human IgG4 (BioRad, Hercules, CA) with unrelated specificities were used for quantification in separate wells on the same plate.
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No products found
because this supplier's products are not listed.
D. Czernecki, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... plasmids were isolated with NucleoSpin Plasmid QuickPure kit (Macherey-Nagel) and suspended in water ...
-
No products found
because this supplier's products are not listed.
Chun-Chun Cheng, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Cells were stained by using Senescence Beta-Galactosidase Staining Kit following 11 days of shRNA (9860, Cell Signaling Technology) according to the manufacture’s protocol ...
-
No products found
because this supplier's products are not listed.
Qing Zhao, et al.,
bioRxiv - Immunology 2023
Quote:
Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01; anti-human IgA, SouthernBiotech, Cat # 2052-01 ...
-
No products found
because this supplier's products are not listed.
Shu Liu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Human primary astrocytes (ScienCell) were cultivated as recommended by ScienCell ...
-
No products found
because this supplier's products are not listed.
Andrew J Kinloch, et al.,
bioRxiv - Immunology 2020
Quote:
... Alexa Fluor 488 goat anti-human IgG and anti-human IgG HRP (Jackson ImmunoResearch).
-
No products found
because this supplier's products are not listed.
Huanhuan Joyce Chen, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The lentiviral vector expressing tetracycline (TET)-inducible shRNA against human P53 was purchased from Gentarget. Inc ...
-
No products found
because this supplier's products are not listed.
Asano Watanabe, et al.,
bioRxiv - Cell Biology 2021
Quote:
... GFP-tagged human vWF plasmid (Romani de Wit et al., 2003) was kindly provided by Jan Voorberg (Stichting Sanquin Bloedvoorziening, Netherland) under Materials Transfer Agreement ...
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No products found
because this supplier's products are not listed.
Denis D. Shi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... human albumin (Bethyl Laboratories ...
-
No products found
because this supplier's products are not listed.
Min Hao, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... the plasmid was extracted using plasmid minipreparation Kit (TIANGEN, #DP103), respectively ...
-
No products found
because this supplier's products are not listed.
Allen T. Yu, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Recombinant human TIAR (Proteintech) was added at indicated concentrations to the probe (∼2 fmol ...
-
No products found
because this supplier's products are not listed.
Anthony A. Ruberto, et al.,
bioRxiv - Genomics 2021
Quote:
... primary human hepatocytes (BioIVT) were seeded 2-3 days prior to infection with 15,000-20,000 sporozoites per well ...
-
No products found
because this supplier's products are not listed.
C López-Haber, et al.,
bioRxiv - Cell Biology 2020
Quote:
Recombinant lentiviruses encoding shRNAs were produced by co-transfection of 293T cells (obtained from American Type Culture Collection, Mannassas, VA) with packaging vectors pDM2.G and pSPAx2 using calcium phosphate precipitation (Marks et al ...
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No products found
because this supplier's products are not listed.
Veena Padmanaban, et al.,
bioRxiv - Cancer Biology 2024
Quote:
Libraries were constructed from 500 ng of total RNAs isolated from 4T1 (scrambled vs Tlr7 shRNA) using the TruSeq RNA Library Prep Kit (Illumina). Constructed libraries were sequenced using Illumina NextSeq (High Output ...
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No products found
because this supplier's products are not listed.
S. M. Nayeemul Bari, et al.,
bioRxiv - Microbiology 2021
Quote:
... Confirmed plasmids were purified using EZNA Plasmid Mini Kit (Omega Bio-tek), and where indicated ...