-
No products found
because this supplier's products are not listed.
Aleksei Kuznetsov, et al.,
bioRxiv - Biochemistry 2020
Quote:
Human recombinant ACE2-His protein (Icosagen OÜ, Estonia, cat# P-302-100) and SARS-CoV-2 Spike protein S1 (Icosagen OÜ ...
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No products found
because this supplier's products are not listed.
Aman Y. Husbands, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Membranes were washed three times with PBS-T and incubated with 1:2000 anti-His-tag antibody (Abiocode M0335-1) for 1h with gentle shaking at RT ...
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No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
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No products found
because this supplier's products are not listed.
Chase P. Kelley, Maja C. Haerle, Eric T. Wang,
bioRxiv - Molecular Biology 2021
Quote:
Recombinant AAV constructs were packaged in AAV6 capsids and purified by SignaGen Laboratories (#SL100865, >1013 VG/mL). Production of AAV-CMV-unreg-CUG1 was attempted on three occasions but titers >109 VG/mL could not be obtained ...
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No products found
because this supplier's products are not listed.
Christopher T. Schafer, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Methoxy e-Coelenterazine (Prolume Purple) was purchased from Nanolight Technologies (Prolume LTD ...
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No products found
because this supplier's products are not listed.
Mu-Sen Liu, et al.,
bioRxiv - Biochemistry 2019
Quote:
... The His6-MBP-Cas9 fusion protein was cleaved to remove the His6-MBP tag by adding the TEV protease to the dialysis tubing (Spectrum labs) during dialysis against buffer containing 20 mM HEPES pH 7.5 ...
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No products found
because this supplier's products are not listed.
Moonjung Jung, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... or αFlag-tag (OAEA00002, Aviva Systems Biology) antibodies.
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No products found
because this supplier's products are not listed.
Sandra D. Chanez-Paredes, et al.,
bioRxiv - Cell Biology 2023
Quote:
... mouse anti-6-His (Bio X Cell, AB_2687790) was used for western blot (3 µg/ml) ...
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No products found
Prathima Radhakrishnan, et al.,
bioRxiv - Microbiology 2022
Quote:
WT E-cad and Null E-cad A431D cells seeded onto fibronectin-coated glass bottom plates (Cellvis; P12-1.5H-N) were infected with L ...
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No products found
because this supplier's products are not listed.
Chunhua Shi, et al.,
bioRxiv - Pathology 2020
Quote:
... Lipopolysaccharide (LPS; from E. coli O111:B4, Cedarlane;) the principal component of Gram-negative bacteria that activates the innate immune system via TLR4 ...
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No products found
because this supplier's products are not listed.
Justin J. Botterill, et al.,
bioRxiv - Neuroscience 2021
Quote:
Virus was delivered using a 500nL Neuros Syringe (#65457-02, Hamilton Company) attached to the stereotaxic apparatus with a probe holder (#751873 ...
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No products found
because this supplier's products are not listed.
Matthew D. Lauver, et al.,
bioRxiv - Immunology 2022
Quote:
... Virus dilutions were combined 1:1 with 0.45% sheep erythrocytes (Innovative Research) and incubated overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Jieqiong Qu, et al.,
bioRxiv - Microbiology 2023
Quote:
... CHIKV virus stock (5 × 108 TCID50/mL) and full human blood (Sanquin) was 1:2 mixed to make the infectious blood meal with a final virus titer of 1.7× 108 TCID50/ml ...
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No products found
because this supplier's products are not listed.
Advika Kamatar, et al.,
bioRxiv - Biophysics 2023
Quote:
... His-Clathrin was labeled with Atto594 NHS-ester (ATTO-TEC, Sigma-Aldrich) according to a previously published protocol (41 ...
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No products found
because this supplier's products are not listed.
Susan A. Leonhardt, et al.,
bioRxiv - Biophysics 2022
Quote:
... virus-containing supernatants were filtered and placed in poly-lysine-coated glass-bottom dishes (MatTek). Particles were then washed and stained with Alexa594-annexin V (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
C Bosch, et al.,
bioRxiv - Neuroscience 2023
Quote:
... resulting in a surface dose of 15.6 e-/nm2) using an OnPoint (Gatan) back-scattered electron detector ...
-
No products found
because this supplier's products are not listed.
Jake W. Anderson, et al.,
bioRxiv - Biochemistry 2023
Quote:
... X-ray diffraction data were collected on a Rigaku FR-E generator (Rigaku Americas Corporation ...
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No products found
because this supplier's products are not listed.
Marcin Poreba, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Fluorescent tags (Cyanine-5 NHS and Cyanine-7 NHS) were purchased from Lumiprobe (Hannover, Germany). Diazomethane was generated according to the Aldrich Technical Bulletin (AL-180 ...
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No products found
because this supplier's products are not listed.
Yanhua Du, et al.,
bioRxiv - Epidemiology 2019
Quote:
The genomes of SFTS virus isolates were compiled using the SeqMan program in the LaserGene software package (DNAStar). The percentage similarities of nucleotide identity or amino acid identity were calculated using the ClustalX software[16] ...
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No products found
because this supplier's products are not listed.
Anna K. Hundsdoerfer, et al.,
bioRxiv - Genomics 2023
Quote:
... euphorbiae (from one head each; the same individuals used for the PacBio genome and Hi-C sequencing) and neural tissue from the internal reference standard Acheta domesticus (female ...
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No products found
because this supplier's products are not listed.
Austin Clyde, et al.,
bioRxiv - Biophysics 2021
Quote:
... The FRET substrate DABCYL-KTSAVLQ↓SGFRKM-E(EDANS) trifluoroacetate salt was purchased from Bachem (PN 4045664), dissolved to 10 mM in DMSO and stored in aliquots at −20 °C ...
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No products found
because this supplier's products are not listed.
Melissa H. Bergeman, et al.,
bioRxiv - Microbiology 2023
Quote:
... Image segmentation and 3D rendering of HSV-1 virus particle undergoing exocytosis from Rab6a vesicle was done in Imaris (Oxford Instruments) by constructing spots and surfaces for the objects at respective image slices ...
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No products found
because this supplier's products are not listed.
Yunye Zhu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Transformants scraped from densely plated transformation plates were inoculated into fresh SC-His medium with 2% raffinose (Amresco, J392) at 0.25 x 107 cells/ml and grown until 0.5-0.8 x 107 cells/ml ...
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No products found
because this supplier's products are not listed.
Brunno R. Levone, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Proteins were quantified using a validated BCA (Bicinchoninic Protein Assay Kit, EuroClone) protocol ...
-
No products found
because this supplier's products are not listed.
April H. Nguyen, et al.,
bioRxiv - Microbiology 2023
Quote:
Protein-protein interactions were evaluated using the Bacterial Two Hybrid System (Euromedex) according to the manufacturer instructions ...
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No products found
because this supplier's products are not listed.
Danya Abazari, et al.,
bioRxiv - Neuroscience 2022
Quote:
Protein palmitoylation assay was performed using CAPTUREome S-palmitoylated protein kit (Badrilla, Leeds, UK), as described by manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... or C1s proteins (Complement Technologies), or BSA (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Sherif Salah, et al.,
bioRxiv - Immunology 2022
Quote:
... and membrane protein peptide (by AMSBIO) were procured ...
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No products found
because this supplier's products are not listed.
Mingyu Fang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... gels were either stained with Coomassie (Protein Ark) or transferred to PVDF membranes for Western blot analysis.
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No products found
because this supplier's products are not listed.
Kevin W. Bollinger, et al.,
bioRxiv - Microbiology 2024
Quote:
... and Ldt3 was raised against purified protein (ProSci). To analyze protein levels by Western immunoblotting ...
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No products found
because this supplier's products are not listed.
Patrik Risteski, et al.,
bioRxiv - Cell Biology 2024
Quote:
... and human anti-centromere protein antibody (Antibodies Incorporated). The secondary antibodies used were donkey anti-mouse IgG-Alexa Fluor 488 (Abcam) ...
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No products found
because this supplier's products are not listed.
Joël S. Bloch, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Protein Complex Suite (NeXtal) and PEG/Ion Suite (Hampton Research). Precipitant solution from crystallization screen was dispensed at 1:1 macromolecular complex/precipitant solution ratio in a total drop volume of 400 nL at room temperature ...
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No products found
because this supplier's products are not listed.
Michael Korenkov, et al.,
bioRxiv - Immunology 2023
Quote:
For protein complexes crystallization we used a mosquito crystallization robot (TTP Labtech) to set vapor diffusion sitting drops with 96-well iQ plates (TTP Labtech) ...
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No products found
because this supplier's products are not listed.
Frederik Fleissner, et al.,
bioRxiv - Biophysics 2020
Quote:
A plasmid coding for human vimentin containing a C-terminal His-tag (EX-D0114-B31, Tebu-bio, Germany) was transformed into E ...
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No products found
because this supplier's products are not listed.
Mukesh Kumar, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and M3 is M1 food supplemented with equal amounts of three saturated TAGs (TAG 42:0; TAG 48:0; TAG 54:0; Larodan).
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No products found
because this supplier's products are not listed.
Nikhil J. Parekh, et al.,
bioRxiv - Immunology 2019
Quote:
IFN-β protein was detected using the mouse Hi-Sensitivity IFN-β ELISA kit (PBL Assay Science). CCL4 protein was detected using the mouse CCL4/MIP-1 beta Quantikine ELISA kit (R&D Systems) ...
-
No products found
because this supplier's products are not listed.
Luis F. Garcia-Alles, et al.,
bioRxiv - Biochemistry 2019
Quote:
... protein tags were removed by incubation overnight at 25°C with turbo TEV protease (GenWay, 5 μg/mL final) in 50 mM Tris pH 8.0 / 300 mM NaCl /2 mM DTT/ 1 mM EDTA ...
-
No products found
because this supplier's products are not listed.
Leonardo M. Molina, et al.,
bioRxiv - Biochemistry 2023
Quote:
... or E-cadherin (1:100) (Biorbyt) antibodies ...
-
No products found
because this supplier's products are not listed.
Ashot Sargsyan, et al.,
bioRxiv - Physiology 2021
Quote:
... Crosslinking was stopped by Quenching Buffer E (Covaris). After washing ...
-
No products found
because this supplier's products are not listed.
Shu Liu, et al.,
bioRxiv - Neuroscience 2022
Quote:
... goat anti- xenotropic MLV virus antibody ABIN457298 (1:1000, antibodies-online); mouse anti- MLV gag ab100970 (1:1000 ...
-
No products found
because this supplier's products are not listed.
Andrew J. Stout, et al.,
bioRxiv - Bioengineering 2022
Quote:
... or Beefy-R (Hi-Def B8 supplemented with 0.4 mg/mL RPI). Beefy-9 and Beefy-R were prepared immediately before use ...
-
No products found
because this supplier's products are not listed.
Aditi Deshpande, et al.,
bioRxiv - Microbiology 2022
Quote:
... library preparation and Hi-Seq 2 x150 paired-end sequencing were performed by GENEWIZ (New Jersey, USA).
-
No products found
because this supplier's products are not listed.
Alasdair G. Kay, et al.,
bioRxiv - Immunology 2021
Quote:
... Sections (5μm) were stained with haematoxylin and eosin (H&E) and mounted in Hydromount (National Diagnostics) as described previously2,3 ...
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No products found
because this supplier's products are not listed.
Andrew M. Dylag, et al.,
bioRxiv - Physiology 2020
Quote:
... Lung slices were stained with Hematoxylin and Rubens Eosin-Phloxine (H&E; Biocare Medical, Concord, CA) and Gomori’s Trichrome (Richard-Allan Scientific ...
-
No products found
because this supplier's products are not listed.
Brenton T. Laing, et al.,
bioRxiv - Neuroscience 2022
Quote:
Immunostaining was conducted for axonal projections and verification of virus expression using a chicken anti-GFP polyclonal antibody (1:1000, Cat # GFP-1020, RRID:AB_10000240; Aves Labs, CA, U.S.A.) or a rabbit anti-DsRed polyclonal antibody (1:1000 ...
-
No products found
because this supplier's products are not listed.
Asha A. Philip, John T. Patton,
bioRxiv - Microbiology 2022
Quote:
... A puc19 plasmid containing a RIX/NSP3-2A-P-His insert under the control of a T7 transcription promoter (puc19/T7/ RIX/NSP3-2A-P-His) was purchased from Bio Basic Canada Inc ...
-
No products found
because this supplier's products are not listed.
Yunlong Li, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... the full-length codon optimized gene with N-terminal 6x-His-sequnce and NdeI-XhoI sites was synthesized (IDT DNA Inc.) (Table S2) ...
-
No products found
because this supplier's products are not listed.
Gabriele Gentile, et al.,
bioRxiv - Zoology 2021
Quote:
... An EBRO® Compact J/K/T/E thermocouple thermometer (model EW-91219-40; Cole-Parmer, Vernon Hills, Illinois, USA 60061) with a T-PVC epoxy-tipped 24 GA probe was used to determine core body temperature ...
-
No products found
because this supplier's products are not listed.
Petra Kangas, et al.,
bioRxiv - Cell Biology 2022
Quote:
... For depletion of the most abundant proteins from CSF ProteoSpin Abundant Serum Protein depletion kit (Norgen Biotek) was used according to manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Deborah L. Gater, et al.,
bioRxiv - Biophysics 2022
Quote:
... Vitamin D binding protein (DBP) was purchased from Athens Research and Vitamin D Binding protein (VDR ...