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No products found
because this supplier's products are not listed.
Andrea M. Chambers, et al.,
bioRxiv - Immunology 2021
Quote:
... Avi-His-Tag biotin labeled antibody (BPS Bioscience) for 30 minutes at 37⁰C ...
-
No products found
because this supplier's products are not listed.
Huan Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... anti-His tag (Solarbio Life Sciences, China, K200060M), anti-toll-like receptor-4 (TLR-4 ...
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No products found
because this supplier's products are not listed.
Luendreo P. Barboza, et al.,
bioRxiv - Neuroscience 2022
Quote:
The pAAV-Mbp-CreERT2 virus (titer: 10×13 GC/μl) was packaged into AAV-PHP.B capsid and purchased from Vector Biolabs (Malvern, PA, cat# VB1545). The AAV-hSyn-hM4D(Gi)-mCherry (cat# VB1545 ...
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No products found
because this supplier's products are not listed.
Yongping Ma, et al.,
bioRxiv - Immunology 2021
Quote:
... 100 µl of rabbit anti-His-tag antibody (1:1000, BioVision, USA, cat#3998) was incubated at 37℃ for 2 h and washed as described above ...
-
No products found
because this supplier's products are not listed.
Patrick J. Flynn, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Centromere Protein E Inhibitor (GSK923295; Cayman Chemicals), Actin Depolymerizer (Cytocholasin-D ...
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No products found
because this supplier's products are not listed.
J. Sebastian Jara, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The amount of p24 capsid protein was determined by the HIV-1 p24 ELISA antigen assay (Beckman Coulter, Fullerton, CA). Virus from different productions averaged 175 ng/µl of p24 antigen.
-
No products found
because this supplier's products are not listed.
Luyao Huang, et al.,
bioRxiv - Plant Biology 2023
Quote:
... the purified recombinant mCherry or Tip6ΔSP-mCherry proteins were separately mixed with purified His-ZmTPL2N-His proteins and incubated with pre-washed magnetic mCherry-Trap beads (Chromotek, Cat. No. rtmak) in wash buffer (0.2 M NaH2PO4/0.2 M Na2HPO4 ...
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No products found
because this supplier's products are not listed.
Robert W. P. Glowacki, et al.,
bioRxiv - Microbiology 2019
Quote:
... Protein purification was accomplished using the pETite N-His vector (Lucigen). PCR primers were designed to amplify products for BT2807 and BT2808 containing all amino acids downstream of the predicted signal peptide sequences ...
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No products found
because this supplier's products are not listed.
James B. Winans, et al.,
bioRxiv - Microbiology 2022
Quote:
... was stained with Anti-6X His Epitope Tag (Rabbit) antibody conjugated to Dylight 405 (Rockland Immunochemicals, cat. # 600-446-382) added to the media at a concentration of .1μg/mL for the entirety of the experiment ...
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No products found
because this supplier's products are not listed.
Jia Chen, et al.,
bioRxiv - Microbiology 2019
Quote:
... The blots were washed with PBS and incubated with primary antibodies (anti-EBV gH/gL (a rabbit polyclonal antiserum;1:200) (45) and anti-His tag antibody (OB05, Calbiochem, 1:1000) for His-tagged KSHV gL ...
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No products found
because this supplier's products are not listed.
Déborah Lécuyer, et al.,
bioRxiv - Microbiology 2023
Quote:
... Envelope defective virus was pseudotyped with HU-1 SARS-CoV-2 Spike protein (pLV-Spike, Invivogen) and used for single round infection assays ...
-
No products found
because this supplier's products are not listed.
Donia Hidaoui, et al.,
bioRxiv - Cancer Biology 2024
Quote:
CUT&Tag assays were performed using CUT&Tag-IT assay kit (Active Motif) on 50,000 CD34+ cells according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Alexandre B. Leitão, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... Sensifast Hi-Rox SyBr kit (Bioline) was used to perform the RT-qPCR on a StepOnePlus system (ThermoFisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Anil Verma, et al.,
bioRxiv - Immunology 2023
Quote:
... were labeled with biotinylated anti-His tag monoclonal antibody (ThermoScientific) and used to capture His-tagged clade C gp120 Du151 protein (Immune Technologies). The gp120-expressing beads were then incubated with triplicate 5-fold dilutions of heat-inactivated serum samples in V-bottom plates for 1h at 37°C ...
-
No products found
because this supplier's products are not listed.
Laurens H. Lindenburg, et al.,
bioRxiv - Biochemistry 2020
Quote:
... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Nikol Dibus, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Purified proteins were then eluted by desthiobiotin using Buffer E (IBA Lifesciences) and subsequently prepared for immunoblotting as described above ...
-
No products found
because this supplier's products are not listed.
Carolin Fritz, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His (MP Biomedicals) plates ...
-
No products found
because this supplier's products are not listed.
Hongzhuang Peng, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... The recombinant human BAP1-FL-WT protein was purchased from Boston Biochem (E-345-050). The Duet-His-ULD/AB protein complex was purified under native purification conditions using Cobalt beads (Talon) ...
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No products found
because this supplier's products are not listed.
Yu-Feng Chien, et al.,
bioRxiv - Bioengineering 2020
Quote:
... a TAG lens (TAG lens 2.5β, TAG Optics) was placed directly on top of an objective (UPLFLN 10XP, Olympus) to enable high-speed axial scan of the focus ...
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No products found
because this supplier's products are not listed.
Alexis S. Gorin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was injected iontophoretically (Nanoject II, Drummond Scientific) via a glass micropipette (tip diameter ∼15-20 μm ...
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No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... his-tagged YFV E protein (Meridian Life Science) was associated to the tips at 5 μg/mL for 60 sec ...
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WB,IF,IP,IHC,FC,ELISA
Cat# A5712, SKU# A5712-20ul,
20ul, $47.00
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Xuxiao He, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and Nickel Magnetic Beads for His tag protein purification (Bimake, B23602) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Jing Zhao, et al.,
bioRxiv - Plant Biology 2023
Quote:
... His-tag antibody (Tiangen, Beijing, China) and GST antibody (Tiangen).
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No products found
because this supplier's products are not listed.
Motokazu Uchigashima, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Tag-blue fluorescent protein (Tag-BFP, Evrogen), Nlgn3Δ ...
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No products found
Fang Wu, et al.,
bioRxiv - Biochemistry 2023
Quote:
The following primary antibodies were used for Western blotting: anti-His Tag (Applied Biological Materials, #G020; 1:4000), anti-HA Tag (Applied Biological Materials ...
-
No products found
because this supplier's products are not listed.
Valery F. Thompson, et al.,
bioRxiv - Biochemistry 2023
Quote:
... to identify FUS protein and anti-His (HIS.H8, Novus Biologicals, NBP2-31055) to identify His tags for both FUS and T7 Pol proteins.
-
No products found
because this supplier's products are not listed.
Vilde Leipart, et al.,
bioRxiv - Biochemistry 2022
Quote:
... We made three sets of blank samples: buffer blank and two sets with SUMO-1 (human, His-tag, Enzo Life Sciences). One collection of SUMO-1 samples were incubated with 25uM ZnCl2 at 4 °C for 1 hour ...
-
No products found
because this supplier's products are not listed.
Vijayendra Dasari, et al.,
bioRxiv - Immunology 2022
Quote:
... protein was concentrated and filtered through Mustang E membrane (PALL Corporation, NY, USA) to further remove endotoxin contaminants ...
-
No products found
because this supplier's products are not listed.
Wanlu Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
... TAG (triacylglycerol, D5-TAG-Mix, LM-6000/D5-TAG 17:0,17:1,17:1, Avanti Polar Lipids), PA (phosphatidic acid ...
-
No products found
because this supplier's products are not listed.
Pieter Nibbering, et al.,
bioRxiv - Plant Biology 2020
Quote:
... 18µl α-Fucosidase (E-FUCTM) and 20µl α-Arabinofuranosidase (E-ABFAN) from Megazyme in a 50 mM sodium acetate buffer pH5 at 37 °C for 4 hours (again ...
-
No products found
because this supplier's products are not listed.
Seungyoon B. Yu, et al.,
bioRxiv - Neuroscience 2023
Quote:
... coverslips were transferred to Hibernate E (BrainBits) medium containing 5nM TMRM as described previously 60 ...
-
No products found
because this supplier's products are not listed.
S.N. Freda, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected using a microsyringe pump (World Precision Instruments); >5 minutes elapsed before the pipette was moved from the ventral injection site to the more dorsal injection site ...
-
No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... The soluble recombinant E protein was recovered by 6-HIS affinity chromatography on Ni-NTA agarose (G-Biosciences) and purified by size exclusion chromatography on a Superdex S200 Increase column (Cytiva).
-
Recombinant Antigen
Cat# REC31653-100,
100µg USD $385.0
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Maya Imbrechts, et al.,
bioRxiv - Immunology 2021
Quote:
... His-tag labeled SARS-CoV-2 RBD (The Native Antigen Company) was biotinylated with the EZ-Link Sulfo-NHS-LC-Biotin kit (Thermofisher Scientific ...
-
No products found
because this supplier's products are not listed.
Hoa Quynh Do, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and lipid-protein nanodiscs (MSP1D1-His-POPC, MSP1D1-His-DMPG, MSP1D1-His-DMPC, Cube Biotech) were used to solubilize in-situ synthesized PCFT ...
-
No products found
because this supplier's products are not listed.
Kaihuai Li, et al.,
bioRxiv - Microbiology 2020
Quote:
... Protein transfer from the Mn2+ phos-tag acrylamide (APExBIO, Houston, USA) gel to the PVDF membrane (Millipore ...
-
No products found
because this supplier's products are not listed.
Oh Sung Kwon, et al.,
bioRxiv - Cell Biology 2020
Quote:
... To repress protein synthesis 100 μg/ml of cycloheximide (TOKU-E) and 300 μM of puromycin (InVivoGen ...
-
No products found
because this supplier's products are not listed.
Ronald L. Shanderson, et al.,
bioRxiv - Cancer Biology 2024
Quote:
Recombinant proteins fused with either Flag or Flag-HA-His tags were transfected at 20 µg per 15 cm plate using Polyethyleneimine 25000 (23966-100, Polysciences) solution at 2.5 µg per 1 µg of DNA ...
-
No products found
because this supplier's products are not listed.
Michael H. C. Buch, et al.,
bioRxiv - Biophysics 2020
Quote:
Purified capsids were mixed with Aurion Anionic 10 nm BSA gold tracers (EMS) at a 1:1 ratio ...
-
No products found
because this supplier's products are not listed.
FWN Chow, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Quantification was carried by western blot analysis in comparison to known concentrations of recombinant full length exWAGO containing an N-terminal 3XFlag-His tag using Image Studio Lite (LI-COR) software ...
-
No products found
because this supplier's products are not listed.
Lydia M. Castelli, et al.,
bioRxiv - Systems Biology 2021
Quote:
... compound E 0.1 µM (Cpd E; Tocris), BDNF 10ng/mL (ThermoFisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Jamie C. Moffa, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was removed from the upper chamber of the concentrator and stored in low protein binding tubes (Eppendorf #022431064) at -80°C.
-
No products found
because this supplier's products are not listed.
Ranganath Maringanti, et al.,
bioRxiv - Cell Biology 2023
Quote:
... with a blue fluorescent protein (BFP)-tag were cultured in smooth muscle cell growth medium-2 (SMGM2) (Lonza). Human umbilical cord vein endothelial cells (HUVEC ...
-
No products found
because this supplier's products are not listed.
Violaine Tribollet, et al.,
bioRxiv - Cell Biology 2020
Quote:
E-plates 16 PET (ACEA Biosciences) were coated with 1.5 µg/cm2 collagen I (Corning ...
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No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a microplate reader (BioTek Synergy HI) to record the absorbance at 650 nm ...
-
No products found
because this supplier's products are not listed.
Jürgen G. Okun, et al.,
bioRxiv - Physiology 2020
Quote:
... or protein-enriched (80%E protein, 10% carbohydrate, 10% fat; D16080303, Research Diets, USA) diets for a period of 3 weeks with various metabolic phenotyping tests ...
-
No products found
because this supplier's products are not listed.
Julian A. Harris, et al.,
bioRxiv - Biochemistry 2021
Quote:
... A single bicistronic baculovirus encoding the human Gβ1 subunit with a N-terminal 6x His-tag and rhinovirus 3C protease site and untagged human Gγ2 subunit was generated using the BestBac method (Expression systems) in Spodoptera frugiperda (Sf9 ...
-
No products found
because this supplier's products are not listed.
Ling He, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
-
No products found
because this supplier's products are not listed.
Juan Jauregui-Lozano, et al.,
bioRxiv - Genomics 2021
Quote:
CUT&Tag was performed using CUTANA™ CUT&Tag reagents (Epicypher, Durham NC ...
-
No products found
because this supplier's products are not listed.
Anthony N. Cutrupi, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 0.1 µM Compound-E (StemCell Technologies), 2ng/mL BDNF ...