-
No products found
because this supplier's products are not listed.
Michal Nemergut, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Data were collected at 293 K with a focused (Confocal Max-Flux, Rigaku) Cu Kα X-ray (1.54 Å) ...
-
No products found
because this supplier's products are not listed.
Evangelos Stefanidis, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Binding of T cell-secreted A4-Fc on OT-I CD47 was assessed by staining with labeled goat anti-mouse IgG Fc (polyclonal, antibodies-online) at 4°C for 30min ...
-
No products found
because this supplier's products are not listed.
Jinye Liang, et al.,
bioRxiv - Microbiology 2023
Quote:
... and imaged on a Li-COR Odyssey Fc scanner (Carver Center for Genomics ...
-
No products found
because this supplier's products are not listed.
Emma T Crooks, et al.,
bioRxiv - Immunology 2021
Quote:
... followed by alkaline phosphatase labeled anti-human Fc conjugate (Accurate Chemicals) and were developed using SigmaFast BCIP/NBT (Sigma).
-
No products found
because this supplier's products are not listed.
Julius Brandenburg, et al.,
bioRxiv - Immunology 2020
Quote:
... 10% of heat-inactivated fetal calf serum (FCS; Pan-Biotek, Aidenbach, Germany), supplemented with 50 ng/ml macrophage colony stimulating factor (M-CSF ...
-
No products found
because this supplier's products are not listed.
Tanadet Pipatpolkai, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Inside-out patches were excised from transfected HEK cells using borosilicate glass pipettes (GC150F-15, Harvard Apparatus; Holliston, MA) pulled to a resistance of 1-3 MΩ ...
-
No products found
because this supplier's products are not listed.
Madeleine F. Jennewein, et al.,
bioRxiv - Immunology 2021
Quote:
... and H3 HK proteins (H3ΔTM H3N2 A/Hong Kong/4801/2014) expressed in 293 cells were purchased from Immune Technology Corp ...
-
No products found
because this supplier's products are not listed.
Valeria Yartseva, et al.,
bioRxiv - Cell Biology 2019
Quote:
... 500 μg BioMag® Goat anti-Human IgG (Fc Specific, Bangs Laboratories #BM562) were washed twice with DPBS without Calcium or Magnesium (Corning #21-031-CV ...
-
No products found
because this supplier's products are not listed.
Yueyuan Shi, et al.,
bioRxiv - Microbiology 2021
Quote:
... All cells were supplemented with 10% (v/v) fetal calf serum (FCS) (ABI) at 37 °C in 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Robert M. Schilke, et al.,
bioRxiv - Immunology 2020
Quote:
... Data analysis was performed using FCS express (Denovo Software) and NovoExpress (Acea Biosciences).
-
No products found
because this supplier's products are not listed.
James P Farmer, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... cell culture medium was removed from T-175cm2 flasks containing confluent adherent Hek-ssβ2-AR and replaced with 10ml Tag-lite medium (LABMED, Cisbio Bioassays) containing 100nM SNAP-Lumi4-Tb (Cisbio Bioassays ...
-
No products found
because this supplier's products are not listed.
Julia E.J. Holm, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Forty-eight hours post transfection cells were removed and incubated in 250 μL/well of HEK Lysis Buffer containing protease Inhibitor Cocktail Set V (Calbiochem) before being centrifuged at 13 000 rpm for 10 min ...
-
No products found
because this supplier's products are not listed.
Souren Paul, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Cycloheximide (CHX) chase assay was performed in transfected HEK 293T cells using treatment of 50 µg/mL CHX (Cayman Chemical) at different time points ...
-
No products found
because this supplier's products are not listed.
Salam Dabsan, et al.,
bioRxiv - Cell Biology 2023
Quote:
RNA was isolated from T-REx-293 and A549 cells using the NucleoSpin RNA Mini kit for RNA purification (Macherey-Nagel). 500 ng of total RNA was used for cDNA synthesis using Maxima™ Reverse Transcriptase (ThermoFisher) ...
-
No products found
because this supplier's products are not listed.
Peeali Mukherjee, et al.,
bioRxiv - Biophysics 2023
Quote:
... Crystals of FlrB-PAS7-108 were obtained by hanging drop vapour diffusion method at 293 K using 24 well crystallization tray with Ammonium Sulfate Grid Screen from Hampton Research with protein of concentration 10 mg ml-1 ...
-
No products found
because this supplier's products are not listed.
Tamina Lebek, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... and resuspended in 10% FCS in PBS with either DAPI (1 μM; Biotium, #40043) or DRAQ7 (300 nM ...
-
No products found
Tamara Miljuš, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
For all experiments human embryonic kidney (HEK) 293SL cells84 were used and regularly tested for mycoplasma contamination (PCR Mycoplasma Detection kit, ABM, Canada). HEK293SL cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) ...
-
No products found
because this supplier's products are not listed.
Lei Gu, et al.,
bioRxiv - Systems Biology 2022
Quote:
... Then the single HEK-293T or HeLa cell were sorted to a 384-well plate containing lysis buffer using the cellenONE® (SCIENION) equipped with the large size Piezo Dispense Capillary (PDC) ...
-
No products found
because this supplier's products are not listed.
Dalia Rosano, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... HEK-293T cells were transfected with cellecta CloneTracker XP library and ready-to-use lentiviral packaging plasmid mix (Cellecta, CPCP-K2A) using Lipofectamine (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Hertzog Maud, et al.,
bioRxiv - Microbiology 2022
Quote:
... The short fluorescent ssDNA substrate used in FCS experiments was prepared with synthetic oligonucleotides (Eurogentec) labeled either with Biotin or Alexa-488 in 5’ in order to generate a Biotin-labeled DNA strand and a fluorescently-labeled DNA strand (Sequence ...
-
No products found
because this supplier's products are not listed.
Cameron O. Zadeh, et al.,
bioRxiv - Biochemistry 2021
Quote:
Horizontal electrophoresis was carried out using the Flatbed Professional (Gel Company Store, FC-EDCProf-2836). The apparatus was maintained at 10°C during electrophoresis ...
-
No products found
because this supplier's products are not listed.
Sylvie Roy, et al.,
bioRxiv - Microbiology 2020
Quote:
... Serial dilutions of known amounts of C-terminally Fc-tagged S2 (BioVendor, Brno, Czech Republic) were used for quantification.
-
No products found
because this supplier's products are not listed.
Lisha Zha, et al.,
bioRxiv - Immunology 2021
Quote:
... RBD was cleaved from the Fc part using thrombin(Beijing Solarbio Science & Technology Co., Ltd.) as described in the manufacturer’s manual.
-
No products found
because this supplier's products are not listed.
Hazel Stewart, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Pacific Blue-conjugated anti-CD71 (Exbio, MEM-75, FC 1 μg / 100 μl / 106 cells), StrepMAB-Classic (IBA LifeSciences ...
-
No products found
because this supplier's products are not listed.
Galen J. Correy, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The cryojet temperature was set to 293 K and crystals were mounted remotely from an SSRL crystallization plate (Mitegen, M-CP-111-095). To assess the susceptibility of crystals to radiation damage ...
-
No products found
because this supplier's products are not listed.
Colin Kremitzki, et al.,
bioRxiv - Genetics 2023
Quote:
Lentiviruses carrying the gRNA libraries were packaged into 8×106 HEK 293T cells/well in a 6-well plate (TPP92006, MIDSCI, Fenton, MO, USA), using the TransIT Lentivirus transfection reagent (MIR 6600 ...
-
No products found
because this supplier's products are not listed.
Stefania Zuppone, et al.,
bioRxiv - Cancer Biology 2023
Quote:
The lipid content of HEK nsEVs after incubation with PBS or carbonate buffer was quantified using a Cholesterol assay (Hansabiomed Life Sciences, Estonia). For this purpose ...
-
No products found
because this supplier's products are not listed.
Darya V. Voronina, et al.,
bioRxiv - Immunology 2022
Quote:
... [48] fused with human IgG1 Fc-fragment and Llama IgG2b hinge were purchased from Evrogen (Russia). CHO-S cells were transfected with VHH-Fc containing construct using the CHO Gro System (Mirus Bio ...
-
No products found
because this supplier's products are not listed.
Louise M. Flanagan, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
... at 180 rpm and 27°C in a spectrophotometric plate reader (TECAN or Multiskan SkanIt FC), with readings every 10 minutes or 1 h respectively ...
-
No products found
because this supplier's products are not listed.
Thomas Clark, et al.,
bioRxiv - Bioengineering 2023
Quote:
... the luciferase activity of SARS-CoV-2 pseudovirus infected HEK 293T cells were determined by luciferase reporter assay kit (eEnzyme, Catalog # CA-L165-10). e ...
-
No products found
because this supplier's products are not listed.
M. Dolores Martin-de-Saavedra, et al.,
bioRxiv - Neuroscience 2019
Quote:
... CNTNAP2-Fc secreted in the medium was affinity purified using ProteinA-CaptivA TM PriMAB (RepliGen, Waltham, MA) and subsequently cleaved with recombinant 3C protease to remove the Fc fragment and leave the mature (i.e. ...
-
No products found
because this supplier's products are not listed.
Lianghui Zhang, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... and levels of the IgG1 Fc moiety were measured by Human IgG ELISA Kit (Immunology Consultants Laboratory). To characterize different routes side-by-side ...
-
No products found
because this supplier's products are not listed.
Xiangyang Liu, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... Cell sorting was conducted using a Sony SH800 cell sorter (Sony Biotech) (condition: FCS threshold=2500, 50% PMT on GFP ...
-
No products found
because this supplier's products are not listed.
Evangelos Stefanidis, et al.,
bioRxiv - Bioengineering 2023
Quote:
... soluble SiRPα-Fc at saturating concentrations were immobilized on 0.4-0.6um Protein G Yellow Fluorescent Particles (PGFP-0552-5, Spherotech) at 4°C for 1h ...
-
No products found
because this supplier's products are not listed.
Swathi Jayaram, Merrit Romeike, Christa Buecker,
bioRxiv - Developmental Biology 2023
Quote:
... Cell pellets were resuspended in PBS to remove residual FCS and counted with a CASY cell counter (Biovendis). Labeling of cells with MULTI-Seq barcodes was performed as described34 ...
-
No products found
because this supplier's products are not listed.
Ruhang Tang, et al.,
bioRxiv - Physiology 2019
Quote:
... Tissue slides were then acetone-fixed followed by incubation with Fc-receptor blocking in 2.5% goat serum (Vector Laboratories) and incubation with primary antibodies cocktail containing anti-CD11b:AlexaFluor488 and CD11c:PE (Biolegend) ...
-
No products found
because this supplier's products are not listed.
Shahanshah Khan, et al.,
bioRxiv - Immunology 2021
Quote:
... Cells were then incubated with Fc block CD16/32 monoclonal antibody (14-0161-82, eBioscience) and stained with primary antibody SARS-CoV-2 (GTX632604, GeneTex) for 30 min on ice ...
-
No products found
because this supplier's products are not listed.
Shikha Yadav, et al.,
bioRxiv - Cell Biology 2023
Quote:
Day 7 BMDMs were washed twice to remove traces of FCS and then incubated in starvation medium from Cell applications Inc ...
-
No products found
because this supplier's products are not listed.
Wezley C. Griffin, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Adherent cells were grown to 40% confluency in 10 cm2 dishes with DMEM/10% FCS supplemented with 10 mM thymidine (TCI America) and cultured at 37 °C with 5% CO2 ...
-
No products found
because this supplier's products are not listed.
M. A. Rossotti, et al.,
bioRxiv - Bioengineering 2021
Quote:
... three-fold dilutions of VHH-Fcs were prepared in V- Bottom 96-well microtiter plates (Globe Scientific, Mahwah, NJ, Cat# 120130) and mixed with 50 µL of CHO-SPK cells ...
-
No products found
because this supplier's products are not listed.
Rebecca O’Cleirigh, Roslyn Gibbs,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... containing 5% (v/v) FCS and incubated overnight in a humidified atmosphere at 37°C and 5% CO2 (Nuaire, DH Autoflow). After 24 hours 1.5mL of the media was removed and media with herb extract or media only control was added ...
-
No products found
because this supplier's products are not listed.
Lindsey M. Brier, Joseph P. Culver,
bioRxiv - Neuroscience 2021
Quote:
... The N=16 mice used for FC matrix computation had stainless steel EEG self-tapping screws (BASI Inc., West Lafayette, IN, USA) fixed at approximately -1mm posterior to bregma ...
-
No products found
because this supplier's products are not listed.
Satoshi Watanabe, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The established stable cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Nacalai Tesque) supplemented with 4∼5% fetal calf serum (FCS; Thermo Fisher Scientific, or Nichirei Biosciences Inc) and 1% penicillin-streptomycin mixed solution (Nacalai Tesque ...
-
No products found
because this supplier's products are not listed.
Vanessa K.A. Silva, et al.,
bioRxiv - Microbiology 2022
Quote:
... fungal cells were blocked with 1% bovine serum albumin (BSA) for 1h at 37°C and incubated respectively with the primary antibody Fc-Dectin 1 (AdipoGen AG-40B-0138) (5 μg/ml ...
-
No products found
because this supplier's products are not listed.
Udoka C. Nwangwu, et al.,
bioRxiv - Zoology 2024
Quote:
... 15 ml Fetal Calf Serum (FCS) 3% and 5ml Amphotericin B) and 500ml of Zirconia beads (Firma Biospec: 2.0 mm, Cat. No 1107912). The contents were macerated using the Qiagen Tissuelyser LT for 10 min and then centrifuged at 4,500 x g for 15 min ...
-
No products found
because this supplier's products are not listed.
Jeremy Krohn, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Primary antibodies were incubated overnight at 4°C in 2% BSA + 10% Fetal Calf Serum (FCS) + 0.3% Triton-X-100: mouse anti GFAP (GFAP; 1:4000; Synaptic Systems Cat# 173 011, RRID:AB_2232308), rabbit anti Mover (Mover ...
-
No products found
because this supplier's products are not listed.
MT Heemskerk, et al.,
bioRxiv - Immunology 2021
Quote:
... diluted in PBS for 10 min at RT and Fc-receptors were subsequently blocked using 5% human serum (HS; Sanquin Blood bank, Amsterdam, The Netherlands) for 45 min at RT ...
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Recombinant Antigen
Cat# REC31697-100,
100µg USD $519.0
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Fred D. Mast, et al.,
bioRxiv - Biochemistry 2021
Quote:
Recombinant Fc-tagged SARS-CoV-2 Spike S1 and S2 proteins purified from HEK293 cells were used for llama immunization (The Native Antigen Company REC31806 and REC31807). For affinity isolation ...
-
Cat# IAR42413749,
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No citation found on bioRxiv
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HVEM-Fc Protein is a recombinant cytokine.
Cat# abx263144-20UG,
20 µg USD $261.0
No citation found on bioRxiv