-
No products found
because this supplier's products are not listed.
D. Azimova, et al.,
bioRxiv - Microbiology 2021
Quote:
... We verified the identity of the proteins in the complex by western blot using native antibody for Cbp1 and a mouse anti-his tag antibody (ABclonal AE003) for Yps3-6xhis.
-
No products found
because this supplier's products are not listed.
Anthony Chifor, Jeongyoon Choi, Joongkyu Park,
bioRxiv - Neuroscience 2021
Quote:
... The protein complex pulled down by GFP-Trap beads (Chromotek) was washed three times with RIPA buffer and eluted in 30 μl of 1x Laemmli sample buffers by heating at 75°C for 10 min.
-
No products found
because this supplier's products are not listed.
Yuki Takamatsu, et al.,
bioRxiv - Microbiology 2019
Quote:
... HEK293 cells (150 cm2 dish) were transfected with 6 μg of each protein-coding plasmid by using TransIT (Mirus). Cell lysis and elution were performed as described above ...
-
No products found
because this supplier's products are not listed.
Harold Marcotte, et al.,
bioRxiv - Immunology 2021
Quote:
... The SARS-CoV-2 S2 N defined peptide pool contains 41 synthetic peptides binding to human HLA derived from the S and N proteins of the SARS-CoV-2 virus (#3620-1, Mabtech AB)58.
-
No products found
because this supplier's products are not listed.
Sonali Jindal, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... human COX2 protein (Cayman Chemical, 60122, 4ng), and 25μg cell line lysates in RIPA buffer were separated by WES automated gel electrophoresis System (Protein Simple ...
-
No products found
because this supplier's products are not listed.
Norie Sugitani, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... supplemented with 1 µM human gp100 (25-33) (Eurogentec) and 50 U/mL IL-2 (PeproTech ...
-
No products found
because this supplier's products are not listed.
Brandon Carter, et al.,
bioRxiv - Immunology 2021
Quote:
A total of nine HLA-A*02:01 human transgenic mice (Jackson Laboratories Number 003475) were used ...
-
No products found
because this supplier's products are not listed.
Xuanwen Li, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Recombinant mouse HEXB protein (His Tag) was obtained from MyBioSource (San Diego, CA). Recombinant proteins alcohol dehydrogenase (ADH ...
-
No products found
because this supplier's products are not listed.
Katarzyna Bogucka-Janczi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... human recombinant GST-fusion ERK3 protein (SignalChem) and E.coli purified ARP3 ...
-
No products found
because this supplier's products are not listed.
Srishti Mandal, et al.,
bioRxiv - Immunology 2023
Quote:
... pulsed with 10µM GP100 peptide (25-33; EGSRNQDWL; Anaspec AS-64752) for 1h ...
-
No products found
because this supplier's products are not listed.
Anna Ilaria Przytula-Mally, et al.,
bioRxiv - Biophysics 2023
Quote:
... for the human CPEB3 complex and PEG/Ion screen (Hampton Research) for the chimpanzee complex ...
-
No products found
because this supplier's products are not listed.
Robert W. P. Glowacki, et al.,
bioRxiv - Microbiology 2019
Quote:
... Protein purification was accomplished using the pETite N-His vector (Lucigen). PCR primers were designed to amplify products for BT2807 and BT2808 containing all amino acids downstream of the predicted signal peptide sequences ...
-
No products found
because this supplier's products are not listed.
Ben S. Ou, et al.,
bioRxiv - Bioengineering 2023
Quote:
... HIS Lite Cy3 Bis NTA-Ni Complex was purchased from AAT Bioquest. Unless otherwise stated ...
-
No products found
because this supplier's products are not listed.
Jerome Xavier, et al.,
bioRxiv - Plant Biology 2022
Quote:
... and PSI complex proteins were Lhcb5purchased from Agrisera. The primary antibody dilutions are as follows ...
-
No products found
because this supplier's products are not listed.
María Belén Palma, et al.,
bioRxiv - Cancer Biology 2021
Quote:
WB analysis was performed to assess the expression of the HLA-G protein in RCC7/HLA-G1 after transfection of the CRISPR/Cas9 system by using the 4H84 mAb (Exbio) at a 1:1000 dilution ...
-
No products found
because this supplier's products are not listed.
Zihao Wang, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... pH 7.0 (The Protein Complex Suite F1, Molecular Dimensions, protein: precipitant ratio 2:1). The crystal was harvested and flash-cooled without adding cryo-protectant ...
-
No products found
because this supplier's products are not listed.
Yanzhou Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged or His-sumo-tagged protein was purified using NiNTA beads (Anatrace, Cat. No. SUPER-NINTA25). Briefly ...
-
No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... C1 complex (Complement Technologies) was injected over flow cells in a two-fold concentration series ...
-
No products found
because this supplier's products are not listed.
Alexander W. Justin, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Green fluorescent protein (GFP) and Red Fluorescent Protein (RFP) human umbilical vein endothelial cells (HUVECs, Promocell), normal human lung fibroblasts (NHLFs ...
-
No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were seeded in 384-well plates (Greiner, #781946) at 30 000 cells/well ...
-
No products found
because this supplier's products are not listed.
Carolyn Rydyznski Moderbacher, et al.,
bioRxiv - Immunology 2022
Quote:
... the hACE2 receptor bound to the S protein was then detected using a mouse anti-His-Tag horseradish peroxidase conjugate (Southern Biotech) and a colorimetric signal generated by the addition of 3,3′,5,5′-TMB substrate ...
-
No products found
because this supplier's products are not listed.
Sarah A. Shelby, Thomas R. Shaw, Sarah L. Veatch,
bioRxiv - Biophysics 2022
Quote:
... VCAM-1/Fc chimeric proteins and anti-human Fc antibodies (recombinant human VCAM-1/Fc chimera protein, Fisher 862-VC-100; Fcγ-specific goat anti-human IgG, Jackson Immunoresearch 109-005-008) or poly-L-lysine (Sigma P4707).
-
No products found
because this supplier's products are not listed.
Jeongsoo Kim, et al.,
bioRxiv - Immunology 2022
Quote:
... H-2Db gp100 Tetramer-EGSRNQDWL-PE (MBL International, Woburn, Massachusetts, USA) staining was performed for 30 min at 4 °C in the dark ...
-
No products found
because this supplier's products are not listed.
Miao-Hsi Hsieh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 S1 protein containing a C-terminal His-tag (Acro; S1N-C52H3) (5 μg/ml) was tagged with anti-His antibody (Genetex; GT395) (1:100 ...
-
No products found
because this supplier's products are not listed.
Yongxing Wang, et al.,
bioRxiv - Immunology 2023
Quote:
... Complex III activity was measured using a Mitochondrial Complex III activity assay kit (BioVision). Complex V activity was measured using a Quantichrom ATPase assay kit (Bioassay Systems).
-
No products found
because this supplier's products are not listed.
Xi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or plastic-bottom (Ibidi: DLD1, HEK293) 96-well plates at densities targeting 60-70% confluence at the time of transfection ...
-
No products found
because this supplier's products are not listed.
Shivneet K. Gill, et al.,
bioRxiv - Biochemistry 2024
Quote:
Protein toxins were incubated with either human sera (Human Serum age 4-6, Innovative Research) or PBS in a 1:1 ratio ...
-
No products found
because this supplier's products are not listed.
Carole Seguin-Devaux, et al.,
bioRxiv - Immunology 2024
Quote:
... or rabbit anti-His (Bethyl, ImTec Diagnostic NV ...
-
No products found
because this supplier's products are not listed.
Steven J. Kunnen, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... in INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT ...
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No products found
because this supplier's products are not listed.
Hongzhuang Peng, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... The recombinant human BAP1-FL-WT protein was purchased from Boston Biochem (E-345-050). The Duet-His-ULD/AB protein complex was purified under native purification conditions using Cobalt beads (Talon) ...
-
No products found
because this supplier's products are not listed.
Kaihuai Li, et al.,
bioRxiv - Microbiology 2020
Quote:
... HtsH2C-HA-His or HtsH3C-Myc-His proteins (100 ng) were incubated with CIAP (Solarbio, Beijing, China) at 28°C for 60 min ...
-
No products found
because this supplier's products are not listed.
Laurens H. Lindenburg, et al.,
bioRxiv - Biochemistry 2020
Quote:
... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Young Bong Choi, et al.,
bioRxiv - Immunology 2021
Quote:
Purified recombinant human TAX1BP1 protein (catalog # P01; Abnova) was incubated with purified GST-tagged IKKi (catalog # PV4875 ...
-
No products found
because this supplier's products are not listed.
Patrik Risteski, et al.,
bioRxiv - Cell Biology 2024
Quote:
... and human anti-centromere protein antibody (Antibodies Incorporated). The secondary antibodies used were donkey anti-mouse IgG-Alexa Fluor 488 (Abcam) ...
-
No products found
because this supplier's products are not listed.
Daniel Flores-Mireles, et al.,
bioRxiv - Biochemistry 2022
Quote:
... decylubiquinol:cytochrome c reductase (complex III) and cytochrome c oxidase (complex IV) by spectrophotometric measurements using a SpectraMax ABS Plus microplate reader (Molecular Devices). Unsealed mitochondrial membranes were thawed on ice and diluted to 100 μg/ml in 25 mM potassium phosphate buffer (pH 7.5 ...
-
No products found
because this supplier's products are not listed.
Travis H. Hand, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... but all reagents (DNA, RNP complex, and Eppendorf tubes) were kept ice cold before reactions were initiated ...
-
No products found
because this supplier's products are not listed.
Shu Liu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Human primary astrocytes (ScienCell) were cultivated as recommended by ScienCell ...
-
No products found
because this supplier's products are not listed.
Hoa Quynh Do, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and lipid-protein nanodiscs (MSP1D1-His-POPC, MSP1D1-His-DMPG, MSP1D1-His-DMPC, Cube Biotech) were used to solubilize in-situ synthesized PCFT ...
-
No products found
because this supplier's products are not listed.
Huang Zhu, Dan S. Kaufman,
bioRxiv - Immunology 2019
Quote:
... 15 % heat-inactivated human AB serum (Valley Biomedical, Catalog # HP1022 HI),1 % P/S ...
-
No products found
because this supplier's products are not listed.
Chongyang Li, et al.,
bioRxiv - Cancer Biology 2019
Quote:
The bacterial expression vectors pReceiver-B11 for His-NSMCE2 and His-PFDN2 recombinant protein expression were purchased from Genecopoeia, Inc (Rockville ...
-
No products found
because this supplier's products are not listed.
Namrata Madan, et al.,
bioRxiv - Genomics 2019
Quote:
... The DNA-protein complex was transferred on positively charged Biodyne nylon membrane (Pall Industries, Fort Washington, NY) using 0.5% TBE for 45 minutes ...
-
No products found
because this supplier's products are not listed.
Carolin Fritz, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His (MP Biomedicals) plates ...
-
No products found
Kirandeep K. Deol, et al.,
bioRxiv - Biochemistry 2020
Quote:
HEK293 Expressing Rpn11-HTBH (Applied Biological Materials, T6007)
-
No products found
because this supplier's products are not listed.
Vilde Leipart, et al.,
bioRxiv - Biochemistry 2022
Quote:
... We made three sets of blank samples: buffer blank and two sets with SUMO-1 (human, His-tag, Enzo Life Sciences). One collection of SUMO-1 samples were incubated with 25uM ZnCl2 at 4 °C for 1 hour ...
-
No products found
because this supplier's products are not listed.
Eleanor M Denham, et al.,
bioRxiv - Immunology 2019
Quote:
... Purified peptide-HLA-A*02 was biotinylated in vitro by BirA enzyme (Avidity) and mixed with Streptavidin:RPE (Bio-Rad #STAR4A ...
-
No products found
because this supplier's products are not listed.
Camille M. Le Gall, et al.,
bioRxiv - Immunology 2021
Quote:
Apheresis material from HLA-A*02:01+ donors were obtained from Sanquin (Nijmegen, NL). PBMC suspension was diluted to 300 mL with RT dilution buffer [PBS + 2 mM EDTA] and split over 10×50 mL conical tubes ...
-
No products found
because this supplier's products are not listed.
Loyda M. Morales Rodríguez, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
HEK293 cells were plated onto 25mm coverslips (Electron Microscopy Sciences, #50949050) coated with poly-D-lysine (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
James D. Hampton, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... For HLA staining heat-induced epitope retrieval buffer 2 (Leica, EDTA pH 8.0) was used ...
-
No products found
because this supplier's products are not listed.
Patrick Blatt, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Rabbit anti-His (1:000, Rockland Inc., 600-401-382), Mouse anti-Fibrillarin (1:25 ...
-
No products found
because this supplier's products are not listed.
Samuel A. Adeleye, Srujana S. Yadavalli,
bioRxiv - Microbiology 2023
Quote:
... The His6-QueE is detected with an anti-His primary antibody and IRDye-conjugated secondary antibody (LI-COR). The protein bands were visualized with the Odyssey CLx imaging system (LI-COR).