-
No products found
because this supplier's products are not listed.
Catarina J. Gaspar, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Cells were transfected with fluorescent reporter constructs (1 μg total DNA/well, 6 well plate) using GenJet (SignaGen Laboratories) according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Jianwen Zhou, et al.,
bioRxiv - Cell Biology 2022
Quote:
GST and GST-fusion proteins were expressed in Escherichia coli strain SoluBL21 (DE3) (Genlantis, #C700200). Protein expression was induced by adding 50 g/ml isopropyl β-D-1- thiogalactopyranoside (IPTG) ...
-
No products found
because this supplier's products are not listed.
Vibeke D. Valderhaug, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 1mg alpha-synuclein monomers (S-1001-1, rPeptide) was resuspended in 1mL MilliQ water ...
-
No products found
because this supplier's products are not listed.
Craig T. Jacobs, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... for fluorescent detection of antibody staining and Draq5 (1:10,000, Biostatus) was used for nuclear staining ...
-
No products found
because this supplier's products are not listed.
Patricia C. Lopes, Robert de Bruijn,
bioRxiv - Animal Behavior and Cognition 2021
Quote:
... Tissue homogenization was done by agitating the tubes for 20□s at a 7□m□s−□1 speed (Beadbug 6 homogenizer, Benchmark Scientific), followed by a 5□min rest period ...
-
No products found
because this supplier's products are not listed.
Jérémy Signoret-Genest, et al.,
bioRxiv - Neuroscience 2022
Quote:
... a 1-s footshock (0.9 mA; Model 2100 Isolated Pulse Stimulator, A-M System) immediately followed the last white noise burst ...
-
No products found
because this supplier's products are not listed.
Abigail J. D’Souza, et al.,
bioRxiv - Neuroscience 2021
Quote:
... samples were diluted to 0.1 μg/μL in provided 1× sample buffer and 1× fluorescent molecular weight marker/reducing agent (Protein Simple). Samples were then vortexed ...
-
No products found
because this supplier's products are not listed.
Kentarou Waki, et al.,
bioRxiv - Immunology 2023
Quote:
... M-IgA was expressed by transfecting a pair of alpha heavy and kappa light chain genes into FreeStyle CHO-S cells that were grown in a 24-well plate according to the manufacturer’s protocol (CHOgro High Yield Expression System, Takara Bio, Shiga, Japan). For large-scale antibody production ...
-
No products found
because this supplier's products are not listed.
Jiayi Liu, et al.,
bioRxiv - Biophysics 2024
Quote:
... or fluorescent PEG atto 633 (SuSoS AG), in the case of macrophages ...
-
No products found
because this supplier's products are not listed.
Cintya E. del Rio Hernandez, et al.,
bioRxiv - Systems Biology 2022
Quote:
... S-aminoethyl-L-cysteine hydrochloride (thyalisine) (Carbosynth), nourseothricin sulfate (Werner BioAgents) ...
-
No products found
because this supplier's products are not listed.
Adam Sychla, et al.,
bioRxiv - Bioengineering 2024
Quote:
... anti-SARS-CoV-S (Absolute Antibodies CR3022), and anti-MBL (SDIX 2445.00.02 ...
-
No products found
because this supplier's products are not listed.
Rahul Kumar, et al.,
bioRxiv - Cell Biology 2023
Quote:
GST-MICAL-L1-CC protein was expressed in Escherichia coli BL21 (500 μM isopropyl β-d-1-thiogalactopyranoside; Wisent Bioproducts; at room temperature for 16 h) and purified using standard procedure in Tris buffer (20 mM Tris ...
-
No products found
because this supplier's products are not listed.
David Chen, et al.,
bioRxiv - Bioengineering 2020
Quote:
... we used a plastic fluorescent slide (Chroma Technologies 92001) and a slide with 4 um fluorescent beads (FocalCheck #1 ...
-
No products found
because this supplier's products are not listed.
Carina C D Joe, et al.,
bioRxiv - Bioengineering 2021
Quote:
... provides shear rate (1516 s−1) and other fluid dynamic parameters similar to those of an 11 m2 filter (ATF10, Repligen) used for perfusion of a 2000 L bioreactor of circa 1500 L working volume under recommended conditions ...
-
No products found
because this supplier's products are not listed.
Ashley M. Fuller, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and were then switched to their respective differentiation medium (C2C12: sodium pyruvate-free DMEM + 2% horse serum + 1% P/S; CnSkMC: Canine Skeletal Muscle Differentiation Medium, Cell Applications, Inc. ...
-
No products found
because this supplier's products are not listed.
Hannah E. Wilson, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... plates at 10,000 cells.well-1 and differentiated in 2% horse serum (Atlanta Biologicals) in DMEM with antibiotics for 48 hours ...
-
No products found
because this supplier's products are not listed.
Agustín Mangiarotti, et al.,
bioRxiv - Biophysics 2023
Quote:
... The fluorescent lipid dye ATTO 647N-DOPE was from ATTO-TEC GmbH (Siegen ...
-
No products found
because this supplier's products are not listed.
Xiaoxuan Su, et al.,
bioRxiv - Microbiology 2021
Quote:
... Full length RNase L gene was synthesized and subcloned into pGEX-4T-3 vector (pGEX-4T-RNaseL-GST) by GENEWIZ as previously described (27).
-
Fluorescent Method
Cat# FPRO200,
FluoProdige fluorescent Dye (10ml) + Dilution Buffer (100ml) + BSA(500µg) lyoph, USD $229.00/KIT
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Olumayokun A Olajide, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and the plate placed on a magnetic plate (OZ Biosciences) and incubated at 37°C for 30 min ...
-
No products found
because this supplier's products are not listed.
Sophia K. Tushak, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Muscle sections were stained with Masson”s trichrome (American MasterTech, Lodi, CA) to identify collagen I deposition ...
-
No products found
because this supplier's products are not listed.
Samuel Okurut, et al.,
bioRxiv - Immunology 2019
Quote:
... and CD21 (Pacific Blue; clone LT21; EXBIO Praha a. s., Czech Republic). CD45 expression was used to discriminate white blood cells in CSF and in blood from Cryptococcus yeast cells ...
-
No products found
because this supplier's products are not listed.
Shaun A. Engelmann, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Backscattered fluorescent signal was directed to a photomultiplier tube (PMT, Hamamatsu, H10770PB-50) with a 775 nm cutoff dichroic (Semrock ...
-
No products found
because this supplier's products are not listed.
Alice Cho, et al.,
bioRxiv - Immunology 2021
Quote:
... coli BL21 (DE3) pLys S cells with pOpen Taw plasmid (Gene and Cell Technologies) and induing the culture overnight with 1 mM IPTG ...
-
No products found
because this supplier's products are not listed.
You Wu, Tam L. Ngyuen, Carrie E. Perlman,
bioRxiv - Physiology 2020
Quote:
... apply a vascular clamp (S&T B1-V, Fine Science Tools, Foster City, CA) between the right middle and caudal lobes and separate the caudal lobe distal to the clamp ...
-
No products found
because this supplier's products are not listed.
Anita K. Nivedha, et al.,
bioRxiv - Biophysics 2021
Quote:
... 96-well white plates (BrandTech). 48 hours later ...
-
No products found
because this supplier's products are not listed.
Mosale Seetharam Sumanth, et al.,
bioRxiv - Biochemistry 2020
Quote:
... were incubated with sAGP-1 or nAGP-1 (25, 50 and 100 μg/ml) in triplicate wells in twelve well cell culture plates (Nest Biotechnology Co. Ltd., China) pre-coated with 0.2% gelatin ...
-
No products found
because this supplier's products are not listed.
Bader M. Jarai, Catherine A. Fromen,
bioRxiv - Bioengineering 2021
Quote:
BMMs in 6-well plates (1×106 cells/well) were detached using Accutase® (Innovative Cell Technologies, Inc.) and washed twice with PBS supplemented with 2% FBS ...
-
No products found
because this supplier's products are not listed.
Claudia Carlantoni, et al.,
bioRxiv - Developmental Biology 2023
Quote:
24-well Plate Softwell Easy Coat hydrogels of different stiffness (0.1, 1, 2, 4 and 25 kPa, Cat #SS24-EC, Matrigen) were coated with 10µg/ml collagen I rat tail diluted in PBS (Cat #A10483-01 ...
-
No products found
because this supplier's products are not listed.
Sana Charaoui-Boukerzaza, et al.,
bioRxiv - Microbiology 2019
Quote:
... using a plate-reader (Scan1200, Interscience).
-
No products found
because this supplier's products are not listed.
Guoli Shi, et al.,
bioRxiv - Microbiology 2022
Quote:
... Titers were measured using an HIV-1 p24 ELISA kit (XpressBio). 50 ng p25 equivalent of virus was added to HeLa-ACE2 cells for 2 hours ...
-
No products found
because this supplier's products are not listed.
Brad A. Palanski, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and the supernatants were transferred to low-binding ultra-performance liquid chromatography vials (Wheaton #11-0000-100-S) and analyzed on a Fusion Lumos mass spectrometer (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Sarah Colijn, Miku Nambara, Amber N. Stratman,
bioRxiv - Developmental Biology 2023
Quote:
Embryos and larvae were anesthetized in buffered 0.2 mg/mL Tricaine-S (Western Chemical, Inc; NC0872873) and mounted in 0.8% low-melting point agarose (IBI Scientific; #IB70056) on a 35-mm glass-bottom petri dish (MatTek ...
-
No products found
because this supplier's products are not listed.
Diana Cortes-Selva, et al.,
bioRxiv - Immunology 2020
Quote:
... plates were coated with 2μg/mL tetanus (List Labs), diphtheria (Sigma) ...
-
No products found
because this supplier's products are not listed.
Charneal L. Dixon, et al.,
bioRxiv - Immunology 2023
Quote:
... cDNA was synthesized from 1 µg RNA using SensiFAST cDNA Synthesis Kit (FroggaBio; Concord, Canada). qPCR was performed using a QuantStudio™ 7 Flex Real-Time PCR System in conjunction with a SybrGreen System (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
Hannah W. Miller, et al.,
bioRxiv - Microbiology 2021
Quote:
... Amoebae were maintained in glass tissue culture tubes at 35°C in TYI-S-33 medium supplemented with 15% heat-inactivated adult bovine serum (Gemini Bio-Products), 2.3% Diamond vitamin Tween 80 solution (40× ...
-
No products found
because this supplier's products are not listed.
Sandra Schwarz, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... A 96-well plate was coated with anti-human CD28 (Immunotools) and 100,000 IFNγ-treated tumor cells were seeded together with 200,000 pTc or TiTc per well in Tc medium containing anti-human CD107a-FITC (Biolegend) ...
-
No products found
because this supplier's products are not listed.
Alia Hassan, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Serum PTH levels were measured using a mouse Intact 1–84 PTH ELISA kit (Quidel, Athens, OH) 12.
-
No products found
because this supplier's products are not listed.
Joseph E Kaserman, et al.,
bioRxiv - Cell Biology 2022
Quote:
Secreted total AAT was quantified from iHep supernatants using the human alpha-1-antitrypsin ELISA quantification kit (GenWay Biotech) per manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Brandon W. Lewis, et al.,
bioRxiv - Immunology 2021
Quote:
... diluted sample MPO was bound to anti-MPO coated plates (Hycult Biotech HK210-01) followed by washes ...
-
No products found
because this supplier's products are not listed.
Chao Yang, et al.,
bioRxiv - Immunology 2020
Quote:
... single cell colonies were picked into 96 well plates by colony picker (Hamilton Company) and cultured for another 2 days for antibody isotyping ...
-
No products found
because this supplier's products are not listed.
Lais L. da Cunha, et al.,
bioRxiv - Microbiology 2022
Quote:
... Primers and small DNA fragments were removed using a 1% low melting agarose gel extraction kit (National Diagnostics, Atlanta, GA, USA). Purification and normalization of amplicons were performed using a SequalPrep plate kit (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Hanyuan Shen, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
A431 cells were treated with different injections for 48 hours in 96-well plates and the cell culture supernatant was collected and tested for the level of IL-1β by ELISA using human interleukin-1 beta ELISA kit (Biosensis, CA, USA) according to the kit protocol ...
-
No products found
because this supplier's products are not listed.
Julie Firmin, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Vit Kit (Irvine Scientific) was used for vitrified embryos ...
-
No products found
because this supplier's products are not listed.
Mark A. Arick II, et al.,
bioRxiv - Genomics 2022
Quote:
A Hi-C library also was prepared using 100 μL of Rohu-1 blood with the Proximo Hi-C Animal Kit (Phase Genomics, Seattle, WA, USA). The final Hi-C DNA-Seq library was submitted to Novogene (www.en.novogene.com ...
-
No products found
because this supplier's products are not listed.
Jeremy F Brooks, et al.,
bioRxiv - Immunology 2020
Quote:
Immunogens were admixed 1:1 with Alhydrogel 1% adjuvant (Accurate Chemical and Scientific Corp.) and injected i.p ...
-
No products found
because this supplier's products are not listed.
Roberta Tesch, et al.,
bioRxiv - Biochemistry 2021
Quote:
A cell suspension of 1,500 cells/50 µl was prepared and pipetted from the topside into a 96 well Perfect 3D Hanging Drop plate (BioTrend). Plates were incubated at 37 °C for several days until hanging drops had developed ...
-
No products found
because this supplier's products are not listed.
Chengyan Wu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The CCK8 kit was purchased from Abbkine. The reactive oxygen detection kit for tissue sections was purchased from Shanghai Pebble Biotechnology Company ...
-
No products found
because this supplier's products are not listed.
Rémi Ronzano, et al.,
bioRxiv - Neuroscience 2024
Quote:
... chicken anti-mCherry (1:1000 to 1:2000, EnCor Biotechnology), mouse IgG2a anti-GAD67 (clone 1G10.2 ...
-
No products found
because this supplier's products are not listed.
Theresa Froehlich, et al.,
bioRxiv - Cell Biology 2023
Quote:
... the mycoplasma kit Venor GeM Classic (Minerva Biolabs) and Taq polymerase (Minerva Biolabs ...
-
No products found
because this supplier's products are not listed.
Keiji Nakamura, et al.,
bioRxiv - Microbiology 2024
Quote:
... As the ELISA kit (RIDASCREEN Verotoxin; R-Biopharm AG) became unavailable in Japan during this study ...